Detection of three pathogenic vibrio species using multiplex polymerase chain reaction (multiplex-PCR) / Umaporn Khimmakthong, Supaporn Noochu
Umaporn Khimmakthong;
Supaporn Noochu
(Thammasat University, 2017 )
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ABSTRACT Vibrio cholera, Vibrio parahaemolyticus and Vibrio vulnificus are opportunistic pathogens causing disease in weak shrimp and possible food poisoning in shrimp consumers. In this study, three pairs of primers were designed to amplify the target DNA fragments of three Vibrio spp. and together with one pair for internal amplification control. The PCR condition was optimized to detect three Vibrio spp. in one reaction tube. The specificity and sensitivity of the reaction were evaluated. The results showed that this technique can detect V. cholera, V. parahaemolyticus and V. vulnificus in the same reaction tube with high specificity. Sensitivity is moderate, 0.5 ng-10 pg. In the future, this technique can be used to detect these bacteria in shrimp. It is potentially useful for shrimp farmers and shrimp consumers. |
| No. Panggil : | 607 STA 22:4 (2017) |
| Entri utama-Nama orang : | |
| Entri tambahan-Nama orang : | |
| Subjek : | |
| Penerbitan : | Pathum Thani: Thammasat University, 2017 |
| Sumber Pengatalogan : | LibUI eng rda |
| ISSN : | 25869000 |
| Majalah/Jurnal : | Science & Technology ASIA |
| Volume : | Vol. 22 No. 4, Oct-Dec 2017: Hal. 11 - 18 |
| Tipe Konten : | text |
| Tipe Media : | unmediated |
| Tipe Carrier : | volume |
| Akses Elektronik : | |
| Institusi Pemilik : | Universitas Indonesia |
| Lokasi : | Perpustakaan UI, Lantai 4, R. Koleksi Jurnal |
| No. Panggil | No. Barkod | Ketersediaan |
|---|---|---|
| 607 STA 22:4 (2017) | 03-18-127058418 | TERSEDIA |
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| Tidak ada ulasan pada koleksi ini: 20470943 |
