RINGKASAN EKSEKUTIF
Ruang Lingkup dan Cara Penelitian: Pengobatan kanker payudara sudahbanyak diupayakan, melalui tindakan bedah, radioterapi, kemoterapidan terapi hormonal, namun hasilnya kurang memuaskan. Yang sangatdidambakan dan ideal ialah cara pencegahan timbulnya kanker atausetidaknya upaya menekan perkembangan kanker. Beberapa jenisbahan makanan telah dilap orkan mempuny ai kh asiat mencegahtimbulnya keganasan . Vitamin A alami maupun sintetik dilaporkandapat mempengaruhi pertumbuhan sel, sehingga \litamin A dosis tinggidiperkirakan dapat mencegah atau menghambat pertumbunan tumor.Dilakukan penelitian eksperimental untuk menilai daya hambat retinilasetat terhadap pertumbuhan tumor transplantabel kele njar susumencit. Tiga kelompok mencit ja tan strain GR, umur ± 2 bulan danberat badan 18 - 23 g, masing-masing 12 ekor, diinokulasi secara subkutandengan 0,2 ml suspensi tumor kelenjar susu yang diperoleh darimencit GR donor. Tiga jam kemudian kelompok perlakuan RA dicekokdengan sonde lambung 0,2 ml larutan retinil asetat 1500 IU, dandila njutkan se tiap li ari selama 14 h a ri. Kelompok kontro l KPmempero leh 0,2 ml akuades sebagai ganti retinil asetat, sedangkankelompok K tidak dibeliikan apa-apa. Daerah inokulasi diraba setiaphari untuk mengetahui pertumbuhan tumor. Volume tumor dan beratbadan mencit diukur setiap 3 haui, dan pada hari ke-15 semua mencitdimatikan dengan cara dislokasi servikal. Tumor diangkat dan diukurvolumenya, lalu dibuat sediaan mikroskopik dengan pewarnaan HE.Hasil dan Kesimpulan: Volume tumor pada mencit kelomQok RA ternyataJebih kecil daripada elompok KP dan K (p < 0,01). Tumor padakedua kelompok kontrol maupun perlakuan RA menunjukkan gambaranadenokarsinoma, namun indeks mitosis pada kelompok RA lebih kecildaripada kedua kelompok kontrol. Dengan demikian dapat disimpulkanbahwa pemberian retinil asetat 1500 IU setiap hari selama 14 haridengan dicekokkan dapat menghambat pertumbuhan tumor transplantabelkelenjar susu mencit GR.Scope and Method of Study: Breast cancer has been treated by variousmeans of surgery, radiotherapy, chemotherapy and hormonal therapy,however, the outcome are still unsatisfactory. The ideal approachshould be through prevention, or at least the development and progressof cancer inhibited. Different kinds of foodstuffs have been reportedto be useful in the protection against malignancy. Vitamin A, eithernatural or synthetic, has been reported to affect cell growth, and ahigh dose was considered to be protective and inhibit tumor growth.An experiment was carried out on male GR mice, approximately 2months old and weighing 18 - 23 g, to evaluate the inhibitory actionof retinyl acetate on the growth of transplantable mammary tumor.Thirty six mice were divided into 3 groups of 12. They were all inoculatedsubcutaneously with a porridge of tumor cells (0.2 ml) preparedfrom a donor mouse. Three hours following inoculation, each of thetreatment group (RA) was given through a gastric tube 1500 IU ofretinyl acetate in 0.2 ml of distilled water, and the treatment continueddaily for 14 days. The control group KP received daily 0.2 ml of distilledwater, and group K was without any treatment. The mice wereobserved daily for tumor growth, and tumor volume and body weightwere measured every three days starting from day 3. At the end of theexperiment (day 15), the mice were sacrificed by cervical dislocation.The tumor was excised from each mouse and the volume measured,and further processed for microscopic examination by HE stain.Findings and Conclusions: The volume of the tumor of the mice receivingretinyl acetate was significantly smaller than those of the controlgroups K and KP (p < 0.01). Tumors from the treatment group as wellas both control groups showed the characteristics of adenocarcinoma,but the mitotic index was significantly smaller in the treatment group.It is concluded that treatment with retinyl acetate, 1500 IU daily for14 days, could inhibit the growth of transplantable mammary tumor inGR mice. |
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