ABSTRAK
Methicillin resistant Staphylococcus aureus (MRSA) merupakan bakteri yang resistenterhadap antibiotik methicillin dan antibiotik golongan β-laktam lainnya. MRSA adalahpatogen umum di rumah sakit dan masyarakat. Isolasi MRSA tidak mudah dilakukankarena seringkali bercampur atau terkontaminasi dengan flora normal seperti coagulasenegative Staphylococci (CoNS) yaitu Staphylococcus epidermidis dan Staphylococcushaemolyticus. Studi ini menggunakan metode fenotipik berupa pengamatan morfologi,pengecatan Gram, Uji biokimia, serta kepekaan antibiotik sedangkan uji genotipik(metode molekular) berupa PCR gen nuc dan mec, SCCmec typing, MLST dansekuensing. Subyek penelitian sebanyak 48 isolat tersimpan di LaboratoriumBakteriologi Molekular, Lembaga Eijkman Jakarta. Diperoleh sebanyak 33 sampel(68.75) memiliki tipe 5 ccr, 9 sampel (18.75) tipe 2 ccr dan 6 sampel (12.5 )nontypeable. Sequence type (ST) yang dominan pada penelitian ini adalah ST239 (2-3-1-1-4-4-3) dan merupakan strain yang multidrug resistant dominan. Pada penelitian inisemua isolat MRSA yang berjumlah 48 isolat telah dikonfirmasi memiliki ciri-cirifenotipik yang sesuai, yaitu Gram positif coccus menyerupai buah anggur, hemolisis,oksidase negatif, katalase positif dan koagulase positif. Sifat bakteri MRSA secaragenotipik mempunyai gen nuc dan gen mecA positif. Hubungan antara sifat genotipedan sifat fenotipe MRSA yang terlihat dalam penelitian ini adalah semua isolat MRSAyang multidrug resistant (uji secara fenotipik) juga merupakan sequence type yangdominan di rumah sakit (uji genotipik).
ABSTRACT
Methicillin resistant Staphylococcus aureus (MRSA) is a bacterium that is resistant tothe methicillin antibiotics and other β-lactam group antibiotics. MRSA is a commonpathogen in hospitals and communities. Isolation of MRSA is not easy to do because itis often mixed or contaminated with normal flora such as coagulase negativeStaphylococci (CoNS), namely Staphylococcus epidermidis and Staphylococcushaemolyticus. This study used phenotypic methods in the form of morphologicalobservations, Gram staining, biochemical tests, and antibiotic sensitivity whilegenotypic tests (molecular methods) in the form of nuc and mec PCR, SCCmec typing,MLST and sequencing. The research subjects were 48 isolates stored in the MolecularBacteriology Laboratory, Eijkman Institute Jakarta. Thirty three samples (68.75) hadtype 5 ccr, 9 samples (18.75) type 2 ccr and 6 samples (12.5) nontypeable. Thedominant sequence type (ST) in this study is ST239 (2-3-1-1-4-4-3) and is a multidrugresistant dominant strain. In this study, all isolates of MRSA, total of 48 isolates, wereconfirmed to have appropriate phenotypic features, which are Gram positive cocciresembling grapes,-hemolysis, negative oxidase, positive catalase and positivecoagulase. Genotypically all isolates have positive nuc gene and mecA gene. Therelationship between genotype features and MRSA phenotype seen in this study is thatMRSA isolates that are multidrug resistant (phenotypic test) are also the dominantsequence types in the hospital (genotypic test). |
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