Full Description
| Cataloguing Source : | LibUI eng rda |
| ISSN : | 25869000 |
| Magazine/Journal : | Science and Technology Asia |
| Volume : | vol. 22, No. 3, July-September 2017 p 20-31 |
| Content Type : | text (rdacontent) |
| Media Type : | unmediated (rdamedia) |
| Carrier Type : | volume (rdacarrier) |
| Electronic Access : | |
| Holding Company : | Universitas Indonesia |
| Location : | Perpustakaan UI, Lantai 4 R. Koleksi Jurnal |
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| Call Number | Barcode Number | Availability |
|---|---|---|
| 607 STA 22:3 (2017) | 03-19-019621892 | TERSEDIA |
| No review available for this collection: 20487620 |
Abstract
ABSTRAK
This study aims to investigate the feasibility of using a minimal glycerol medium with inorganic salt supplementation for a low-cost dihydroxyacetone (DHA) production by bacteria. Acetic acid bacterial isolates of Gluconobacter and Asaia, which are found in the Northern, North-Eastern, Middle, Western, and Southern regions of Thailand, were evaluated for their ability to produce DHA using glycerol as a carbon source. During the qualitative screening, 66 isolates, including Gluconobacter (61 isolates) and Asaia (5 isolates), from a total of 486 isolates showed highly positive results by the Fehling test. The 5 isolates of Asaia gave low DHA production in the quantitative screening, whereas Gluconobacter isolates showed DHA production at low (0-5.70 g/L), medium (5.71-11.40 g/L), and high (11.41-16.89 g/L) levels. Preliminary culture medium optimizations for G. frateurii BCC 36199, a most promising microorganism for DHA production, were also carried out using a low-cost minimal glycerol medium supplemented with an inorganic salt. G. frateurii BCC 36199 produced 18.67 g/L of DHA with ysp of 95.44% (DHA moles/glycerol moles) at 30°C, 20 g/L of glycerol, and pH 4.5. The cultivation of G. frateurii BCC 36199 in the developed minimal glycerol medium is practical and can be further optimized in order to apply for industry.
This study aims to investigate the feasibility of using a minimal glycerol medium with inorganic salt supplementation for a low-cost dihydroxyacetone (DHA) production by bacteria. Acetic acid bacterial isolates of Gluconobacter and Asaia, which are found in the Northern, North-Eastern, Middle, Western, and Southern regions of Thailand, were evaluated for their ability to produce DHA using glycerol as a carbon source. During the qualitative screening, 66 isolates, including Gluconobacter (61 isolates) and Asaia (5 isolates), from a total of 486 isolates showed highly positive results by the Fehling test. The 5 isolates of Asaia gave low DHA production in the quantitative screening, whereas Gluconobacter isolates showed DHA production at low (0-5.70 g/L), medium (5.71-11.40 g/L), and high (11.41-16.89 g/L) levels. Preliminary culture medium optimizations for G. frateurii BCC 36199, a most promising microorganism for DHA production, were also carried out using a low-cost minimal glycerol medium supplemented with an inorganic salt. G. frateurii BCC 36199 produced 18.67 g/L of DHA with ysp of 95.44% (DHA moles/glycerol moles) at 30°C, 20 g/L of glycerol, and pH 4.5. The cultivation of G. frateurii BCC 36199 in the developed minimal glycerol medium is practical and can be further optimized in order to apply for industry.