Optimization and validation study of HPLC with fluorescence detection method for analysis of benzo[c]fluorine
Piyada Songsermsaku, Kazuichi Hayakawa (Thammasat University, 2018)
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ABSTRACT Optimization and method validation of HPLC with fluorescence detector for the determination of benzo[c]fluorene were studied. The results showed that benzo[c]fluorene had a maximum excitation wavelength at 309 nm and a maximum emission wavelength at 354 nm. The mixture of acetonitrile-water was used as a mobile phase at a ratio of 85:15. The standard curve was linear over the concentration range of 0.5-50 ng/ml. The limit of detection and the limit of quantification were 0.27 ng/ml and 0.90 ng/ml, respectively. The relative standard deviation of precision for intra-day and inter- days were less than 5% for both low (5 ng/ml) and high (50 ng/ml) concentration of benzo[c]fluorene. The method was validated and showed satisfactory results of linearity, precision, limit of detection, and limit of quantification. Accuracy in terms of percent recovery strongly depends on sample matrix. Therefore, further studies are needed to investigate the accuracy when the method is applied in the real sample such as environmental and food samples. |
No. Panggil : | 670 STA 23:2 (2018) |
Entri utama-Nama orang : | |
Entri tambahan-Nama orang : | |
Penerbitan : | Pathum Thani: Thammasat University, 2018 |
Sumber Pengatalogan : | LibUI eng rda |
ISSN : | 25869000 |
Majalah/Jurnal : | Science & Technology ASIA |
Volume : | Vol. 23, No.2 April-June 2018: Hal. 46-53 |
Tipe Konten : | teks |
Tipe Media : | unmediated |
Tipe Carrier : | volume (rdacarier) |
Akses Elektronik : | https://www.tci-thaijo.org/index.php/SciTechAsia/article/view/131202 |
Institusi Pemilik : | Universitas Indonesia |
Lokasi : | Perpustakaan UI, Lantai 4, R. Koleksi Jurnal |
No. Panggil | No. Barkod | Ketersediaan |
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670 STA 23:2 (2018) | 03-19-259037854 | TERSEDIA |
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