Pengaruh Nanokurkumin terhadap kadar antioksidan endogen jaringan hati pada model kanker ovarium yang mendapat cisplatin pada tikus = Effects of Nanocurcumin on hepatic endogenous antioxidants in cisplatin-treated rat ovarian cancer model
Muhammad Farrasy Ammar;
Melva Louisa, supervisor; Ari Estuningtyas, examiner; Melva Louisa, examiner
(Fakultas Kedokteran Universitas Indonesia, 2020)
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Latar Belakang: Cisplatin, agen kemoterapi utama dalam terapi kanker ovarium,memiliki sifat hepatotoksik karena menginduksi stres oksidatif. Kurkumin dapatmeningkatkan kadar atau aktivitas antioksidan endogen seperti enzim superoksidadismutase dan glutation. Formulasi nanopartikel kurkumin dapat meningkatkanbioavailabilitas kurkumin dan distribusinya pada organ target. Penelitian inibertujuan untuk mengetahui pengaruh nanokurkumin terhadap hepatotoksisitasakibat cisplatin melalui regulasi antioksidan endogen SOD dan GSH. Metode: 25ekor tikus galur Wistar betina dibagi ke dalam 1 kelompok sham dan 4 kelompokmodel kanker ovarium yang diinduksi DMBA pada penelitian in-vivo ini. Empatkelompok tersebut adalah kelompok tanpa terapi, cisplatin 4 mg/KgBBintraperitoneal, cisplatin dengan kurkumin konvensional 100 mg/KgBB per oral,atau cisplatin dengan nanopartikel kurkumin dalam kitosan 100 mg/KgBB per oral.Setelah perlakuan selama 1 bulan, hepar tikus diambil dan disimpan beku.Pengukuran aktivitas SOD, kadar GSH, dan kadar GSSG dilakukan dengan metodespektrofotometri. Hasil: Uji statistik pada kadar GSH, GSSG, dan aktivitas SODmenunjukkan peningkatan yang signifikan pada kelompok ko-kemoterapikurkumin konvensional dibanding monoterapi cisplatin (p<0.05). Tidak adaperbedaan yang bermakna antarkelompok pada rasio GSH/GSSG (p>0.05) dantidak ditemukan perbedaan bermakna antara kedua kelompok ko-kemoterapi padasemua variabel (p>0.05). Kesimpulan: Kurkumin konvensional dan nanokurkuminsetara dalam meregulasi antioksidan endogen SOD dan GSH pada tikus modelkanker ovarium yang mendapat cisplatin. Introduction: As the primary chemotherapeutic agent of choice for ovarian cancer,cisplatin has hepatotoxic properties via oxidative stress induction. Curcumin canincrease the levels and activities of endogenous antioxidants like superoxidedismutase enzyme and glutathione. Formulation of curcumin nanoparticlesincreases its bioavailability and target organ distribution. This research aims toelucidate the effects of nanocurcumin on cisplatin-induced hepatotoxicity viaregulation of endogenous antioxidants, SOD and GSH. Method: 25 Wistar femalerats were grouped into 1 sham group and 4 DMBA-induced ovarian cancer modelgroups in this in-vivo study. Four cancer model groups were further divided intono-treatment, 100 mg/KgBW intraperitoneal cisplatin therapy, cisplatin with oral100 mg/KgBW conventional curcumin, and cisplatin with oral 100 mg/KgBWcurcumin nanoparticle in chitosan group. The liver of the rats were taken and frozenafter one month of treatment. Spectrophotometry was used to measure the activitiesof SOD, levels of GSH, and levels of GSSG. Results: Statistic tests on levels ofGSH, GSSG, and activity of SOD showed significant increase in the curcumin cochemotherapyagainst cisplatin monotherapy (p<0.05). There was no significantdifference within the groups of GSH/GSSG ratio (p>0.05) and no significantdifference was found between the curcumin co-chemotherapy and nanocurcuminco-chemotherapy groups in all the variables (p>0.05). Conclusion: Conventionalcurcumin and nanocurcumin administration are similar in regulating endogenousantioxidants SOD and GSH on rats with ovarian cancer model treated with cisplatin. |
 S-Muhammad Farrasy Ammar.pdf :: Unduh
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| No. Panggil : | S-pdf |
| Entri utama-Nama orang : | |
| Entri tambahan-Nama orang : | |
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| Penerbitan : | Jakarta: Fakultas Kedokteran Universitas Indonesia, 2020 |
| Program Studi : |
| Bahasa : | ind |
| Sumber Pengatalogan : | LibUI ind rda |
| Tipe Konten : | text |
| Tipe Media : | computer |
| Tipe Carrier : | online resource |
| Deskripsi Fisik : | xiv, 47 pages : illustration + appendix |
| Naskah Ringkas : | |
| Lembaga Pemilik : | Universitas Indonesia |
| Lokasi : | Perpustakaan UI |
| No. Panggil | No. Barkod | Ketersediaan |
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| S-pdf | 14-22-50242696 | TERSEDIA |
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| Tidak ada ulasan pada koleksi ini: 20514033 |
