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Pengaruh Ekstrak Etanol Daun Annona Muricata terhadap Perubahan Morfologi Ginjal dari Mencit Galur Swiss Webster yang diinduksi dengan Aloksan = The Effect of Annona Muricata Leaves Ethanol Extract on The Morphological Changes of Alloxan-Induced Swiss Webster Mice’s Kidney

Mutiara Intan Permata Sari; Supri Irianti Handayani, supervisor; Dewi Sukmawati, examiner (Fakultas Kedokteran Universitas Indonesia, 2021)

 Abstrak

Pendahuluan: Diabetes melitus (DM) merupakan gangguan metabolik kronik yang
ditandai dengan hiperglikemia. Salah satu komplikasi DM adalah disfungsi ginjal. Daun
sirsak (Annona muricata) memiliki potensi sebagai antidiabetes. Namun, belum ada
penelitian mengenai ekstrak etanol daun Annona muricata terhadap morfologi ginjal
terutama terkait proliferasi dan pertumbuhan dari sel mesangial glomerulus, ruang
Bowman, dan tubulus kontortus proksimal. Penelitian ini bertujuan untuk mengetahui
pengaruh ekstrak etanol daun Annona muricata terhadap perubahan histopatologi ginjal
dari mencit Swiss Webster yang diinduksi Aloksan. Metode: Penelitian ini menggunakan
studi eksperimental in vivo. Sampel penelitian berasal dari organ ginjal 30 ekor mencit
jantan galur Swiss Webster yang diinduksi aloksan. Sampel dibagi menjadi kelompok
kontrol negatif, kontrol positif, dan 3 kelompok perlakuan yang diberikan ekstrak etanol
daun sirsak (EEDS) dengan masing-masing dosis 150, 300, dan 600 mg/kgBB selama 14
hari. Semua sampel preparat jaringan ginjal diwarnai dengan pewarnaan PAS dan diamati
pada perbesaran 400x dengan melihat pada 10 LP. Pengukuran histopatologi dilakukan
dengan software MIimageView. Hasil: EEDS dalam semua dosis perlakuan tidak
berpengaruh secara signifikan pada diameter glomerulus dan tebal tubulus kontortus
ginjal serta lebar ruang bowman ginjal kiri dan tubularisasi glomerulus ginjal kanan.
EEDS dosis 600 mg/kgBB berpengaruh secara signifikan (p<0,05) pada lebar ruang
bowman ginjal kanan. EEDS dosis 600 mg/kgBB dan kontrol positif secara signifikan
(p<0,05) dapat mencegah tubularisasi glomerulus ginjal kiri. Kesimpulan: Pemberian
EEDS selama 14 hari tidak berpengaruh pada ukuran diameter glomerulus dan tebal
tubulus kontortus proksimal ginjal kiri dan kanan serta lebar ruang bowman ginjal kiri
dan tubularisasi glomerulus ginjal kanan. Namun, pemberian EEDS dosis 600 mg/kgBB
dapat mencegah penyempitan ruang bowman ginjal kanan dan tubularisasi glomerulus
ginjal kiri.

Introduction: Diabetes mellitus (DM) is a chronic metabolic disorder characterized by
hyperglycemia, one complication of which includes kidney disfunction. Soursop leaves
(Annona muricata) is known to have antidiabetic potential; however, no research has been
conducted regarding the effect of ethanol extract of Annona muricata leaves on the
morphology of the kidney, particularly the proliferation and growth of the mesangial cells
of glomerulus, Bowman’s space, and the proximal convoluted tubule. This study aimed
to observe the effect of Annona muricata leaves ethanol extract on the morphological
chanes of Alloxan-Induced Swiss Webster Mice’s Kidney. Method: This study was
conducted as an in vivo experimental study. The samples were obtained from the kidneys
of 30 alloxan-induced male Swiss Webster mice, which were then grouped into negative
control, positive control, and 3 experimental groups given ethanol extracts of soursop
leaves (EEDS) with a dose of 150, 300, and 600 mg/kgBW for 14 days. The kidney tissue
samples were stained using the PAS staining and observed with a 400x magnification on
10 LP. The histopathological measurement was conducted using the MIimageView
software. Results: EEDS in all dose of treatment showed no significance on the
glomerular diameter and the thickness of the proximal convoluted tubules of the kidney,
as well as the width of the Bowman’s space of the left kidney and glomerular
tubularization of the right kidney. EEDS with a dose of 600 mg/kgBW showed a
significant effect (p<0.05) on the height of the right kidney’s Bowman’s space. EEDS
with a dose of 600 mg/kgBW and the positive control significantly (p<0.05) prevented
the tubularization of the left kidney’s glomerulus. Conclusion: The 14 days treatment
with EEDS didn’t have a significant difference on the glomerular diameter and the
thickness of the proximal convoluted tubules of the left and right kidney, as well as the
height of the Bowman’s space of the left kidney and the right kidney’s glomerular
tubularization. However, EEDS with a dose of 600 mg/kgBW could prevent the
narrowing of the Bowman’s space of the right kidney and the glomerular tubularization
of the left kidney.

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 Metadata

No. Panggil : S-pdf
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Subjek :
Penerbitan : Jakarta: Fakultas Kedokteran Universitas Indonesia, 2021
Program Studi :
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : computer
Tipe Carrier : online resource
Deskripsi Fisik : 67 pages : illustration ; appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI
  • Ketersediaan
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No. Panggil No. Barkod Ketersediaan
S-pdf 14-22-84822737 TERSEDIA
Ulasan:
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