Pendahuluan: Diabetes melitus (DM) merupakan gangguan metabolik kronik yangditandai dengan hiperglikemia. Salah satu komplikasi DM adalah disfungsi ginjal. Daunsirsak (Annona muricata) memiliki potensi sebagai antidiabetes. Namun, belum adapenelitian mengenai ekstrak etanol daun Annona muricata terhadap morfologi ginjalterutama terkait proliferasi dan pertumbuhan dari sel mesangial glomerulus, ruangBowman, dan tubulus kontortus proksimal. Penelitian ini bertujuan untuk mengetahuipengaruh ekstrak etanol daun Annona muricata terhadap perubahan histopatologi ginjaldari mencit Swiss Webster yang diinduksi Aloksan. Metode: Penelitian ini menggunakanstudi eksperimental in vivo. Sampel penelitian berasal dari organ ginjal 30 ekor mencitjantan galur Swiss Webster yang diinduksi aloksan. Sampel dibagi menjadi kelompokkontrol negatif, kontrol positif, dan 3 kelompok perlakuan yang diberikan ekstrak etanoldaun sirsak (EEDS) dengan masing-masing dosis 150, 300, dan 600 mg/kgBB selama 14hari. Semua sampel preparat jaringan ginjal diwarnai dengan pewarnaan PAS dan diamatipada perbesaran 400x dengan melihat pada 10 LP. Pengukuran histopatologi dilakukandengan software MIimageView. Hasil: EEDS dalam semua dosis perlakuan tidakberpengaruh secara signifikan pada diameter glomerulus dan tebal tubulus kontortusginjal serta lebar ruang bowman ginjal kiri dan tubularisasi glomerulus ginjal kanan.EEDS dosis 600 mg/kgBB berpengaruh secara signifikan (p<0,05) pada lebar ruangbowman ginjal kanan. EEDS dosis 600 mg/kgBB dan kontrol positif secara signifikan(p<0,05) dapat mencegah tubularisasi glomerulus ginjal kiri. Kesimpulan: PemberianEEDS selama 14 hari tidak berpengaruh pada ukuran diameter glomerulus dan tebaltubulus kontortus proksimal ginjal kiri dan kanan serta lebar ruang bowman ginjal kiridan tubularisasi glomerulus ginjal kanan. Namun, pemberian EEDS dosis 600 mg/kgBBdapat mencegah penyempitan ruang bowman ginjal kanan dan tubularisasi glomerulusginjal kiri.
Introduction: Diabetes mellitus (DM) is a chronic metabolic disorder characterized byhyperglycemia, one complication of which includes kidney disfunction. Soursop leaves(Annona muricata) is known to have antidiabetic potential; however, no research has beenconducted regarding the effect of ethanol extract of Annona muricata leaves on themorphology of the kidney, particularly the proliferation and growth of the mesangial cellsof glomerulus, Bowman’s space, and the proximal convoluted tubule. This study aimedto observe the effect of Annona muricata leaves ethanol extract on the morphologicalchanes of Alloxan-Induced Swiss Webster Mice’s Kidney. Method: This study wasconducted as an in vivo experimental study. The samples were obtained from the kidneysof 30 alloxan-induced male Swiss Webster mice, which were then grouped into negativecontrol, positive control, and 3 experimental groups given ethanol extracts of soursopleaves (EEDS) with a dose of 150, 300, and 600 mg/kgBW for 14 days. The kidney tissuesamples were stained using the PAS staining and observed with a 400x magnification on10 LP. The histopathological measurement was conducted using the MIimageViewsoftware. Results: EEDS in all dose of treatment showed no significance on theglomerular diameter and the thickness of the proximal convoluted tubules of the kidney,as well as the width of the Bowman’s space of the left kidney and glomerulartubularization of the right kidney. EEDS with a dose of 600 mg/kgBW showed asignificant effect (p<0.05) on the height of the right kidney’s Bowman’s space. EEDSwith a dose of 600 mg/kgBW and the positive control significantly (p<0.05) preventedthe tubularization of the left kidney’s glomerulus. Conclusion: The 14 days treatmentwith EEDS didn’t have a significant difference on the glomerular diameter and thethickness of the proximal convoluted tubules of the left and right kidney, as well as theheight of the Bowman’s space of the left kidney and the right kidney’s glomerulartubularization. However, EEDS with a dose of 600 mg/kgBW could prevent thenarrowing of the Bowman’s space of the right kidney and the glomerular tubularizationof the left kidney. |
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