Potensi Asam Amino L-Arginine Berbagai Konsentrasi terhadap Proliferasi Human Dental Pulp Stem Cells (hDPSCs) = Potential Use of Amino Acid L-Arginine Towards Human Dental Pulp Stem Cells (hDPSCs) Proliferation
Rizka Andini Pratiwi;
Anggraini Margono Rompas, supervisor; Aryo Megantoro, supervisor; Aditya Wisnu Putranto, examiner; Endang Suprastiwi, examiner; Sylva Dinie Alinda, examiner
(Fakultas Kedokteran Gigi Universitas Indonesia, 2022)
|
|
Latar Belakang: Pada kondisi inflamasi pulpa, terdapat penurunan dari jumlah protein dan asam amino. L-Arginine adalah asam amino semi-esensial karena berperan penting pada kondisi tertentu, gangguan imun berat dan luka bakar, yang membutuhkan asupan tambahan L-Arginine eksternal. Asam amino L-Arginine menjadi satu-satunya substrat sintesis nitric oxide (NO) dan poliamina berasal dari konversi L-Arginine menjadi ornithinen melalui arginase. NO dan poliamina merangsang proliferasi sel dan memiliki efek positif pada perkembangan melalui siklus sel. Tujuan: Mengetahui potensi asam amino L-Arginine terhadap proliferasi hDPSCs. Metode: Evaluasi asam amino L- Arginine konsentrasi 300, 400, 500 μmol/L, serta DMEM sebagai kontrol terhadap proliferasi hDPSCs menggunakan uji cell count setelah 24 jam. Analisis statistic menggunakan Oneway ANOVA dengan post hoc Bonferroni. Hasil: Terdapat perbedaan bermakna potensi L-Arginine 300,400 dan 500 μmol/L dibandingkan kontrol, dan L- Arginine 500 μmol/L memiliki rerata proliferasi hDPSCs paling tinggi sebesar 436.666 sel/ml. Kesimpulan: Asam amino L-Argininee memiliki potensi terhadap proliferasi hDPSCs dan proliferasi tertinggi pada asam amino L-Arginine konsentrasi 500 μmol/L. Background: In the inflammatory condition of the pulp, there is a decrease in the amount of protein and amino acids. L-Arginine is a semi-essential amino acid because it plays an important role in certain conditions, severe immune disorders and burns, which require additional intake of external L-Arginine. The amino acid L-Arginine is the sole substrate for the synthesis of nitric oxide (NO) and polyamines derived from the conversion of L- Arginine to ornithine via arginase. NO and polyamines stimulate cell proliferation and have a positive effect on progression through the cell cycle. Objective: To determine the potential of L-Arginine amino acid on the proliferation of hDPSCs. Methods: Evaluation of L-Arginine amino acid with concentrations of 300, 400, 500 μmol/L, and DMEM as a control for hDPSCs proliferation using cell count test after 24 hours. Statistical analysis using Oneway ANOVA with Bonferroni post hoc. Results: There was a significant difference in the potency of L-Arginine 300,400 and 500 μmol/L compared to control, and L-Arginine 500 mol/L had the highest average proliferation of hDPSCs of 436.666 cells/ml. Conclusion: The amino acid L-arginine has the potential to proliferate hDPSCs and the highest concentration at 500 μmol/L. |
 T-Rizka Andini Pratiwi.pdf :: Unduh
|
| No. Panggil : | SP-pdf |
| Entri utama-Nama orang : | |
| Entri tambahan-Nama orang : | |
| Entri tambahan-Nama badan : | |
| Subjek : | |
| Penerbitan : | Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2022 |
| Program Studi : |
| Bahasa : | ind |
| Sumber Pengatalogan : | LibUI ind rda |
| Tipe Konten : | text |
| Tipe Media : | computer |
| Tipe Carrier : | online resorce |
| Deskripsi Fisik : | xv, 54 pages : illustration + appendix |
| Naskah Ringkas : | |
| Lembaga Pemilik : | Universitas Indonesia |
| Lokasi : | Perpustakaan UI |
| No. Panggil | No. Barkod | Ketersediaan |
|---|---|---|
| SP-pdf | 16-23-07405843 | TERSEDIA |
| Ulasan: |
| Tidak ada ulasan pada koleksi ini: 20522098 |
