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To test the effect of one point mutation on the first initiation codon of Chicken Anemia Virus (CAV) open reading frame-3 (ORF-3), an apoptin knocked out expressor plasmid pCLS-VP3(-) and a CAV apoptin knocket out plasmid pCAV/Ap(-) were constructed. In both plasmids, the first ATG in ORF-3 was mutated into...
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