N-asetilglukosamin merupakan monosakarida derivat glukosa yangmemiliki banyak fungsi dan terdapat secara luas dalam sistem tubuh manusia. Nasetilglukosamin dimanfaatkan secara luas baik dibidang kesehatan maupunkosmetik. Produksi N-asetilglukosamin secara enzimatis menggunakan kitinaseyang salah satunya dapat diisolasi dari bakteri relatif lebih ramah lingkungan danmenghasilkan rendemen yang tinggi. Penelitian ini bertujuan untuk mendapatkankondisi optimal produksi N-asetilglukosamin secara hidrolisis enzimatikmenggunakan kitinase yang diisolasi dari bakteri. Seleksi dilakukan terhadapsembilan kultur koleksi BPPT untuk mendapatkan isolat potensial yang dapatmenghasilkan kitinase dengan aktivitas terbaik dan dapat menghidrolisis kitinmenjadi N-asetilglukosamin dengan rendemen tertinggi. Diantara isolat tersebut,BPPT CC 2 menunjukkan aktivitas kitinase terbaik serta dapat menghidrolisis substrat koloidal kitin dan menghasilkan N-asetilglukosamin dengan rendemen tertinggi. Produksi N-asetilglukosamin menggunakan kitinase BPPT CC 2dioptimasi pH, suhu, konsentrasi substrat dan enzim serta lamanya inkubasi.Rendemen N-setilglukosamin tertinggi sebanyak 99,41% didapatkan darihidrolisis 3% substrat koloidal kitin dengan 0,2 U enzim pada kondisi pH 6,0 dansuhu 500C selama 5 hari. Hasil ini mengindikasikan bahwa kitinase dari BPPT CC2 dapat digunakan untuk biokonversi kitin menjadi N-asetilglukosamin denganrendemen yang tinggi untuk kepentingan industri.

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Abstract
N-acetylglucosamine is a monosaccharide derivative of glucose thatserve a number of functions and are widely distributed throughout the humanbody system. N-acetylglucosamine posses benefit as a nutritional supplement fortherapeutic usage and also in cosmetics. Enzymatic hydrolysis using chitinaseisolated from bacterial, as one of the enzyme source, produce high yield N- acetylglucosamine and environmental friendly. This research is aimed to achieveoptimum condition for N-acetylglucosamine production by enzymatic hydrolysisusing bacterial isolated chitinase. Nine isolates from BPPT culture collection wasselected to get the most potential isolate, which produced chitinase with bestactivity and able to hydrolyze colloidal chitin resulting high yield of N-acetylglucosamine. Among those isolates, isolate BPPT CC 2 showed the bestchitinase activity and able to hydrolyze colloidal chitin substrate resulting highyield of N-acetylglucosamine. Production of N-acetylglucosamine with BPPT CC2 chitinase was optimized by adjusting its pH, temperature, substrate and enzyme concentration, and also time of hydrolysis. The yield of 99,41% as maximum production of N-acetylglucosamine was obtained by hydrolysis of 3% substrate colloidal chitin with 0,2 U chitinase after 5 days of incubation on pH 6,0 and temperature of 500C. This result suggest that crude chitinase produced by BPPTCC 2 could be useful for bioconversion of chitin into high yield N-acetylglucosamine for industrial application.