ABSTRACTThe study investigated the soxhlet extraction of Leum Pua rice bran oil (Oryza sativa L.) using 12 hours of extraction time, with comparison of product with hexane and ethanol as solvent. The chemical properties and gamma-oryzanol content in the rice bran oil were investigated. The results showed that the highest yield of rice bran oil was obtained from extraction using 70:30 %v/v ethanol:water solvent with percent yield of 21.89±0.35 g (mean+SD)/100g of dry rice bran) and maximum amount of gamma-oryzanol of 0.4647 mg/L of rice bran oil. The chemical properties and gamma-oryzanol content of Leum Pua rice bran oil indicates acceptable quality of oil when stored for a long time causing foul odour due to lipid peroxidation.