The effect of Dimethyl Formamide (DMF) as a cryoprotectant on spermatozoa quality of carp fish Cyprinus carpio race Majalaya 24 hours post-cryopreservation was studied. This study was conducted in the Environmental Engineering Laboratory. Puspiptek. Serpong. Sperm was collected by the hand stripping method and were immediately diluted with extender [6J and cryoprotectant (DMF). The final concentrations of DMF were 0. 3.75. 7.5. and ! 1.25%. respectively. Sperm were frozen in liquid nitrogen for 24 hours. All sperm were re-examined after thawing for motility. viability, and abnormality. The highest percentage of the molility and the viability of preserved sperm was showed or the DMF 7.5%. On the oiher hand the lowest percentage of the abnormality of preserved sperm was demonstrated by the DMF 7.5%. also. Accordingly. DMF 7.5% is the optimum levels that could preserved spermatozoa still in a good quality 24 hours posl-cryopreservation.