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"Pendahuluan: Rosela memiliki khasiat antiinflamasi. Ulser merupakan keadaan inflamasi dalam rongga mulut.Tujuan: Menganalisis efektivitas ekstrak etanol terstandar kelopak bunga rosela terhadap penyembuhan ulser.Metode: Penelitian in vivo pada model ulser 12 tikus, dibagi menjadi kelompok kontrol dan perlakuan. Rosela diteteskan 2 kali sehari pada ulser, kemudian hari ke 3 dan 7 dikorbankan. Parameter yang diobservasi adalah kemerahan dan bengkak di sekitar jaringan ulser, diameter ulser, serta skor radang.Hasil: Rosela 7.5% 7 hari dapat menghilangkan kemerahan, sedangkan 3.75% dan 7.5% menurunkan diameter ulser dan skor radang.Kesimpulan: Aplikasi tetes ekstrak terstandar rosela efektif dalam mempercepat penyembuhan ulser.

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Background: Roselle has antiinflammatory effect. Ulcer is an inflammatory condition in oral.Objective: Analyze effectiveness of roselle in healing an ulcer.Methods: This research in vivo on ulcer model 12 rats, divided into control and treatment group. Roselle drops were applied twice a day on ulcer, then day 3 to 7 was sacrificed. Healing process was observed by looking redness and swelling, ulcer diameter, and inflammation score.Results: Roselle 7.5% 7 days can eliminate redness, while 3.75 % and 7.5% reduced ulcer diameter and inflammation score.Conclusion: Application drops of standardized ethanol extract of roselle are effective in ulcer healing."

"Pendahuluan : Rosela dilaporkan memiliki efek antiinflamasi.Tujuan: Menganalisis efektivitas ekstrak etanol terstandar kelopak bunga rosela oles terhadap penyembuhan ulser mukosa mulut.Metode: Penelitian ini in vivo pada 12 tikus S-D dengan ulser mukosa mulut. Kelompok penelitian adalah kontrol dan perlakuan. Ekstrak 7.5% dan 15% dioleskan dua kali sehari, pada hari ke-4 dan ke-8, tikus dikorbankan. Parameter yang diobservasi adalah diameter, kemerahan dan pembengkakan di jaringan sekitar ulser, serta skor radang.Hasil: Pada kelompok perlakuan, diameter ulser dan skor radang menurun, tidak terdapat bengkak dan merah pada jaringan di sekitar ulser.Kesimpulan: Ekstrak 7.5% efektif menyembuhkan ulser mukosa mulut.

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Background: Roselle has anti-inflammatory effect.Objective: Analyze effectiveness of standardized ethanol extract of Roselle topical towards oral mucous ulcer.Method: In vivo study was created with ulcer on 12 rats. Research group divided into control and test group. Standardized roselle 7.5% and 15% were applied topically twice a day. On the 4th and 8th days, rat were sacrificed. Parameters are ulcer diameter, reddish color and swollen membrane, and inflammation score of ulcer.Result: Test group indicated ulcer diameter and inflammation score were reduce, no swelling and redness observed.Conclusion: Standardized roselle 7.5% is effective for ulcer healing of oral mucous."

"Latar Belakang: Rosela dilaporkan sebagai agen antiradang.Tujuan: menganalisis efektivitas ekstrak etanol terstandar kelopak rosela terhadap penyembuhan ulser mukosa mulut.Metode: Dua belas tikus dibagi dalam kelompok kontrol dan perlakuan. Ekstrak 3.75% dan 7.5% dioleskan 2 kali sehari pada mukosa mulut. Pengamatan dilakukan selama 3 dan 7 hari. Kemerahan dan pembengkakan jaringan sekitar diamati secara visual. Diameter ulser diukur satu hari setelah dibuat dan sebelum dikorbankan. Spesimen dibuat, diamati secara mikroskopis, dan dilakukan skoring.Hasil: Diameter ulser dan skor radang menurun. Kemerahan dan pembengkakan jaringan sekitar tidak terlihat lagi.Simpulan: Ekstrak 7.5% efektif terhadap penyembuhan ulser mukosa mulut tikus.

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Background: Roselle is reported as anti-inflammatory agent.Objectives: To analyze standardized roselle calyces ethanol extract efficacy`s towards oral mucous ulcer.Methods: Twelve rats were divided into 2 groups; control and experimental group. The 3.75% and 7.5% extract were applied topically twice a day in oral mucous. Animals were observed for 3 and 7 days. Redness and swelling were observed visually. Ulcer diameter was measured a day after creation and before sacrifice. Specimens were observed microscopically and scored.Results: Ulcer diameter and inflammation score were decreased. No redness and swelling observed.Conclusions: The 7.5% extract is effective for the ulcer."

"Latar Belakang: Rosela dilaporkan memiliki khasiat sebagai antiinflamasi.Tujuan: mengetahui efektivitas rosela terhadap ulser mukosa mulut.Metode: 12 tikus sprague-dawley dibagi dua; kelompok kontrol dan perlakuan. Triamcinolone diaplikasikan pada kelompok kontrol positif, kelompok perlakuan diberikan aplikasi tetes rosela 7.5% dan 15%, selama 3 atau 7 hari.Hasil; Hilangnya kemerahan dan pengurangan diameter ulser pada rosela 7 hari (7.5% dan 15%), hilangnya bengkak pada rosela 7 hari (15%) dan penurunan skor radang pada rosela 3 hari (15%) dan 7 hari (7.5% dan 15%).Kesimpulan: Efektivitas rosela setara Triamcinolone 0.1% pada kemerahan dan penurunan diameter ulser 7 hari.

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Backgrounds: Roselle have effect as anti-inflammatory.Objectives: To determine the effectiveness of roselle towards oral mucosa ulcer.Methods: 12 sprague-dawley rats were divided into 2 groups; control and treatment. triamnicolone as positive control, treatment group was dropped by 7.5% and 15% roselle. Wound healing was observed in the third or seventh day.Results; Redness disappearance and ulcer diameter decrease in seventh days (7.5% and 15%). Swelling disappearance in seventh days (15%). Inflammation score decrease in both third days (15%) and seventh days (7.5% and 15%).Conclusions: roselle is effective for ulcer healing. the Effectiveness is equivalent to Triamcinolone 0.1% in redness disappearance and decreasing of ulcer diameter in seventh days."

"Ulser sering ditemukan di mukosa mulut. Rosella dilaporkan dapat mempercepat penyembuhan ulser. Tujuan: Menganalisis efektivitas ekstrak etanol kelopak bunga rosella teridentifikasi 3.75%. Metode: sebanyak 36 ekor tikus dibagi 3 kelompok paparan saline, triamcinolone acetonide, ekstrak rosella 3.75%. Tikus dikorbankan pada harike 2, 4, 8, dan 15. Spesimen difiksasi dan dilakukan skoring pada sediaan mikroskopik. Hasil: Pemaparan saline menunjukkan skor radang 3; pemaparan triamcinolone acetonidepada dua ekor tikus menunjukkan skor radang 1, satu ekor tikus menunjukkan skor radang 2; pemaparan ekstrak rosella 3.75% menunjukkan skor radang 2. Kesimpulan: Pemaparan ekstrak rosella 3.75% kurang efektif menyembuhkan ulser mukosa mulut.

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Ulcers are often found in oral mucosa. Roselle has been reported to accelerate wound healing.

Objectives: To analyze the effectiveness of identified ethanol extract of roselle calyx 3.75%.

Methods: A total of 36 rats were divided into 3 groups of application, saline, triamcinolone acetonide, and roselle extract 3.75%. Rats were sacrificed on 2nd, 4th, 8th, and 15th days. Specimens were made and microscopic slide were scored.

Results: Saline application showed inflammation score 3. Triamcinolone acetonide application showed inflammation score 1. Roselle extract application showed inflammation score 2.

Conclusion: Application of roselle extract 3.75% less effective to accelerate wound healing."

"ABSTRAKLatar Belakang: Rosela mempunyai efek anti-inflamasi yang mempercepat proses penyembuhan. Fibroblas berperan penting dalam proses penyembuhan. Tujuan: Mengevaluasi hasil proses penyembuhan ulser mukosa mulut tikus berdasarkan ekspresi fibroblas secara imunohistokimia pascapaparan ekstrak etanol rosela 15%. Metode: Sampel ulser mukosa mulut tikus pascapaparan kelompok kontrol dan kelompok perlakuan diwarnai dengan antibodi TE-7(immunohistokimia). Parameter yang digunakan sel positif dan intensitas warna (pixel). Hasil: Terdapat peningkatan sel positif pada kelompok rosela 15% dihari ke-3 (skor 2) dengan intensitas warna menunjukan skor kuat (<125 pixel) pada hari ke-1, -3, -7, -14. Kesimpulan: Terdapat peningkatan ekspresi fibroblas pada proses penyembuhan ulser mukosa mulut tikus pascapaparan ekstrak etanol rosella 15%.

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ABSTRACTBackgrounds:Roselle have an anti-inflammatory effect that can accelerate wound healing. Fibroblast play a critical role in wound healing process. Objectives: To evaluate the result of wound healing process towards rat oral mucous ulcer based on fibroblast expression in immunohistochemistry after exposure 15% ethanol roselle extract. Methods: rat oral mucous ulcer samples after exposure the control group and the treatment group that stained by TE-7 antibody (immunohistochemistry). Parameters that used are positive cells and color intensity (pixels). Results: There was an increase of positive cells in group 15% roselle on the 3rd day (score 2) with color intensity indicates high score (<125 pixels) from the 1st, 3rd, 7th and 14th days. Conclusions: Fibroblast expression increased in wound healing process of rat oral mucous ulcer after exposure 15% ethanol roselle extract"

"ABSTRAKLatar Belakang : Kelopak bunga Rosela dilaporkan memiliki efek antiinflamasi.TE-7 adalah salah satu penanda fibroblas. Tujuan: Menganalisis ekspresi fibroblaspada penyembuhan ulser mukosa mulut tikus pascapaparan ekstrak etanol kelopakbunga Rosela 7,5 % secara imunohistokimia. Metode: Blok parafin dari ulsermukosa mulut tikus yang terdiri dari kelompok perlakuan dan kelompok kontroldipulas secara imunohistokimia tidak langsung menggunakan TE-7 sebagai antibodiprimer. Interpretasi ekspresi fibroblas berdasarkan skoring. Hasil : Pada hari ke-3,kelompok perlakuan menunjukkan skor 2 dan kedua kelompok kontrol menunjukkanskor 1. Kesimpulan: Setelah 3 hari paparan ekstrak etanol kelopak bunga Roselateridentifikasi 7,5% fibroblas terekspresi lebih baik daripada kelompok kontrol.

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ABSTRACTBackground: Roselle’s calyces reported have anti inflammation. TE-7 is one offibroblasts marker. Objective: Analyzing fibroblasts expression on rat oral mucosaulcer healing after exposure 7.5% ethanol extract of Roselle calyces byimmunohistochemistry. Method: Paraffin blocks of rat oral mucosa ulcer consistingof the treatment group and the control group stained indirect immunohistochemistrymethod using TE-7 as the primary antibodi Result: On day 3, the treatment groupshowed a score of 2 and the two control groups showed a score of 1. Conclusion:After 3 days exposure to 7,5% ethanol extract of Roselle calyces, fibroblastsexpressed better than the control group."

"Rosella berkhasiat anti-inflamasi dan anti-bakteri. Tujuan : Mengetahui kondisi makroskopis, mikroskopis ulser mukosa yang diaplikasikanekstrak rosella. Metode : Tiga puluh enam tikus sprague-dawley dibagi, Triamcinolone Acetonide 0.1% sebagai kontrol positif, saline 0.9% sebagai kontrol negatif, ekstrak rosella 7.5% sebagai kelompok perlakuan, selama 1, 3, 7, 14 hari. Hasil : pada perlakuan 7 hari, selisih diameter awal dan akhir kelompok ekstrak rosella 7.5% lebih besar dibandingkan TA dan saline, skor radang ulser kelompok ektrak rosella 7.5% lebih rendah dibandingkan TA dan saline. Kesimpulan : Aplikasi ekstrak rosella 7.5% efektif mempercepat penyembuhan ulser pada perlakuan 7 hari.

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Roselle have anti-inflammatory, anti-bacteria effect.

Objectives : To determine macroscopic, microscopic condition of ulcers after application of roselle extract.

Methods : thirty six sprague-dawley rats were divided, TA 0.1% as positive control, saline 0.9% as negative control, roselle extract 7.5% as treatment group. healing was observed in the first, third, seventh, and fourteenth day.

Result : In 7 days, the diameter differences of ulcers applied with roselle extract 7.5% is bigger and inflammation score of ulcers is lower than TA and saline.

Conclusion : application of roselle extract 7.5% is effective in accelerating healing in 7 days."

"ABSTRAKLatar Belakang: Rosela dilaporkan memiliki efek antiinflamasi dan efektif dalampenyembuhan ulser. Fibroblas memiliki peranan penting dalam penyembuhanluka. Namun, mekanisme yang mengontrol pertumbuhan dan proliferasi fibroblasmasih kurang dipahami. Imunohistokimia TE-7 merupakan salah satu metodeyang dapat digunakan untuk mewarnai fibroblas pada jaringan blok parafin.Tujuan: Untuk menganalisa peranan pemeriksaan imunohistokimia TE-7 padapenyembuhan model ulser pascapaparan ekstrak etanol kelopak bunga roselateridentifikasi. Metode: 12 blok parafin dari model ulser dibagi menjadi 2kelompok: kelompok kontrol dan perlakuan. Blok parafin selanjutnya di prosesdan dipulas dengan imunohistokimia TE-7. Pengamatan pada slide dan skoring.Hasil: pada hari ke 1 dan 3 saline, TA dan rosela menunjukkan skor 1. Pada harike 7 saline menunjukkan skor 2, TA menunjukkan skor 3 dan rosela menunjukkanskor 1. Pada hari ke 14, salin dan rosela menunjukkan skor 1, TA menunjukkanskor 2. Kesimpulan: Pemeriksaan imunohistokimia TE-7 dapat digunakan untukmengidentifikasi fibroblas.

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ABSTRACTBackground: Roselle had been reported as anti-inflammation agent and effectivein ulcer healing. Fibroblasts played an important role in wound healing. But, thereis a lack of understanding about mechanism that control the growth andproliferation of fibroblast. Immunohistochemistry TE-7 is one of method couldbe used to stain fibroblast in paraffin-embedded tissue sample. Objectives: Toanalyzed the role of immunohistochemistry in wound healing of models ulcerpost-exposure of identified roselle calyx. Method: Twelve paraffin embeddedtissue of models ulcer were processing and staining with immunohistochemistryTE-7. Microscopic slide were observed and scored. Result: On day 1st and 3rd,saline, TA and roselle group showed scored 1. On day 7th, saline showed score 2;TA showed score 3 and roselle showed score 1. On day 14th, saline and roselleshowed score 1; TA showed score 2. Conclusion: Immunohistochemicalexamination antibody TE-7 could be used to identify fibroblast in wound healingof model ulcers."

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Latar Belakang: Periodontitis disebabkan oleh infeksi mikroba sepertiStreptococcus sanguinisyang mengganggu respon imun dan integritas jaringan pendukung gigi.Ekstrak etanol kelopak bunga rosela (Hibiscus sabdariffa Linn.) memiliki kandungan antimikrobial terhadap bakteri Gram-positif seperti S. sanguinis. Untuk mengembangkan bentuk sediaan, dibuat gel ekstrak etanol kelopak bunga rosela.Tujuan: Mengetahui efektivitas antibakteri gel ekstrak etanol kelopak bunga rosela terhadap S. sanguinis. Metode: Studi in vitro metode difusi agar yang mengukur zona hambat (mm) gel rosela 10%, 15%, dan 25% yang dipaparkan pada agar Mueller Hinton terinokulasi bakteri S. sanguinis, diinkubasi 6 jam, serta metode total plate count (CFU/mL) untuk menghitung jumlah koloni hidup bakteri S. sanguinis setelah terpapar gel rosela 10%, 15%, dan 25%. Kontrol positif yaitu gel klorheksidin 0,2% dan kontrol negatif yaitu gel basis tanpa zat aktif. Hasil: Zona hambat gel ekstrak etanol kelopak bunga rosela konsentrasi 15% dan 25% berbeda bermakna dibandingkan kontrol (p<0,05), gel konsentrasi 10% tidak menghasilkan zona hambat. Ketiga konsentrasi gel secara signifikanmenurunkan jumlah koloni bakteri dibandingkan kontrol (p<0,05). Efek penghambatan terbesar terdapat pada gel konsentrasi 25%. Kesimpulan: Gel ekstrak etanol kelopak bunga rosela memiliki efek hambat terhadap bakteri S. sanguinis.

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Background: Periodontitis is caused by microbial infection, such as Streptococcus sanguinisthat disturbs immune response and the integrity of tooth-supporting tissue. Roselle (Hibiscus sabdariffa Linn.) calyx ethanol extract has antibacterial properties against Gram-positive bacteria, including S. sanguinis. In order to develop the dosage form, roselle calyx ethanol extract gel was made. Objective:To observe the antibacterial effectiveness of roselle calyx ethanol extract gel against S. sanguinis. Method: in vitrostudy using disk diffusion method which measures clear zone of inhibition (mm)of 10%, 15%, and 25% roselle gel applied on Mueller Hinton agar inoculated with S. sanguinis, incubated for 6 hours, and total plate count method which counts the number of living S. sanguiniscolonies (CFU/mL)after being exposed to 10%, 15%, and 25% roselle gel. Positive control is 0.2% chlorhexidine gel and negative control is gel without active substances.Result: Inhibitory zones of 15% and 25% roselle gel have significant differences compared controls (p<0.05), 10% roselle gel did not show inhibitory zones.All three concentrations of gel significantly reduced the number of colonies compared to controls (p<0.05). Highest inhibitory effect was observed in 25% roselle gel.Conclusion: roselle calyx ethanol extract gel showed inhibitory effect against S. sanguinis.

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