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"[ABSTRAKCuka apel telah lama digunakan untuk menekan nafsu makan dengan tujuan untuk menurunkan berat badan. Namun, belum banyak bukti penelitian ilmiah yang membuktikan pengaruh penekanan nafsu makan tersebut. Dengan demikian peneliti melakukan sebuah studi eksperimental dengan hipotesis yang akan diuji adalah efektivitas cuka apel dalam menurunkan nafsu makan tikus tidak sebaik yang dimiliki mazindol. Jenis tikus yang digunakan sebagai sampel penelitian adalah tikus Sprague Dawley dengan berat 190- 250 gram. Sampel berjumlah 24 ekor dan dibagi dalam 3 kelompok, yaitu kelompok kontrol (sebagai kontrol negatif), kelompok mazindol (sebagai kontrol positif), dan kelompok cuka apel. Semua sampel mendapat perlakuan yang sama. Nafsu makan, yang ditunjukkan oleh berat sisa makanan yang diamati selama 2 minggu. Data berat sisa makanan dianalisis menggunakan uji hipotesis One Way Anova dan uji Post Hoc. Peneliti mendapati ada perbedaan bermakna secara statistik berat sisa makanan antara kelompok kontrol dan kelompok cuka apel (p = 0,000) dengan uji One Way Anova dan (p = 0,000) dengan uji Post Hoc. Sementara itu, tidak adanya perbedaan bermakna terlihat pada kelompok kontrol dan mazindol (p = 0,623)dengan uji Post Hoc. Dengan demikian disimpulkan efektivitas cuka apel dalam menurunkan nafsu makan lebih baik daripada mazindol.

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ABSTRACTApple cider vinegar has beend used for ages in suppressing appetite in order to lose weight. Meanwhile, there are only few valid studies regarding this matter. Thereby, researcher conducted an experimental study and define the effectiveness of apple cider vinegar is not as good as mazindol for suppressing appetite as the hypothesis. Researcher used Sprague Dawley strain rats which weight are 190-250 grams as research sample. Twenty four rats devided into 3 groups, that is control group (as negative control), mazindol group (as positive control), and apple cider vinegar group. All samples given same treatment. Appetite assessed by the weight of leftovers which has been monitored for 2 weeks. The data of leftovers analyzed by One Way Anova and Post Hoc hyphoteses tests. Researcher found that there is significance difference of leftovers between control and apple cider vinegar group (p = 0,000) using One Way Anova test and (p = 0,000) using Post Hoc test. Meanwhile there is no significance difference between control and mazindol group (p = 0,623) using Post Hoc hypothesis test. In conclusion, the effectiveness of apple cider vinegar in suppressing appetite is better than mazindol.;Apple cider vinegar has beend used for ages in suppressing appetite in order to lose weight. Meanwhile, there are only few valid studies regarding this matter.Thereby, researcher conducted an experimental study and define the effectiveness of apple cider vinegar is not as good as mazindol for suppressing appetite as the hypothesis. Researcher used Sprague Dawley strain rats which weight are 190-250 grams as research sample. Twenty four rats devided into 3 groups, that is controlgroup (as negative control), mazindol group (as positive control), and apple cider vinegar group. All samples given same treatment. Appetite assessed by the weight of leftovers which has been monitored for 2 weeks. The data of leftovers analyzed by One Way Anova and Post Hoc hyphoteses tests. Researcher found that there is significance difference of leftovers between control and apple cider vinegar group (p = 0,000) using One Way Anova test and (p = 0,000) using Post Hoc test.Meanwhile there is no significance difference between control and mazindol group (p = 0,623) using Post Hoc hypothesis test. In conclusion, the effectiveness of apple cider vinegar in suppressing appetite is better than mazindol., Apple cider vinegar has beend used for ages in suppressing appetite in order to lose weight. Meanwhile, there are only few valid studies regarding this matter.Thereby, researcher conducted an experimental study and define the effectiveness of apple cider vinegar is not as good as mazindol for suppressing appetite as the hypothesis. Researcher used Sprague Dawley strain rats which weight are 190-250 grams as research sample. Twenty four rats devided into 3 groups, that is controlgroup (as negative control), mazindol group (as positive control), and apple cider vinegar group. All samples given same treatment. Appetite assessed by the weight of leftovers which has been monitored for 2 weeks. The data of leftovers analyzed by One Way Anova and Post Hoc hyphoteses tests. Researcher found that there is significance difference of leftovers between control and apple cider vinegar group (p = 0,000) using One Way Anova test and (p = 0,000) using Post Hoc test.Meanwhile there is no significance difference between control and mazindol group (p = 0,623) using Post Hoc hypothesis test. In conclusion, the effectiveness of apple cider vinegar in suppressing appetite is better than mazindol.]"

"Belum banyak bukti ilmiah yang mendukung efektivitas cuka apel. Namun, penggunaannya sebagai agen penurun berat badan telah semakin meluas. Bahkan, di berbagai iklan penjualan produk, suplemen makanan ditampilkan seolah-olah memiliki manfaat seperti obat. Untuk itu, peneliti mengadakan penelitian eksperimental dan hipotesis yang akan diuji adalah efektivitas cuka apel tak sebaik yang dimiliki obat standar dalam menurunkan berat badan tikus strain Sprague Dawley. Tikus Sprague Dawley dengan berat 190- 250 gram digunakan sebagai sampel dalam penelitian ini. Ada 24 ekor tikus yang terbagi ke dalam 3 kelompok, yaitu kelompok kontrol (sebagai kontrol negatif), kelompok mazindol (sebagai kontrol positif), dan kelompok cuka apel. Ketiganya mendapat jenis makanan dan minuman yang sama. Berat badan dan tingkah laku tikus diamati selama dua minggu. Data berat badan sebelum dan sesudah terapi diambil dan dianalisis menggunakan uji hipotesis One Way Anova. Peneliti mendapati tidak adanya penurunan berat badan pada ketiga kelompok perlakuan. Akan tetapi, berat badan tikus terus mengalami peningkatan dari hari ke hari. Data peningkatan berat badan tersebut menunjukkan adanya perbedaan bermakna secara statistik antara kelompok kontrol dan kelompok cuka apel (p = 0,012). Sementara itu, tidak adanya perbedaan bermakna terlihat baik antara kelompok kontrol dan mazindol maupun kelompok mazindol dan cuka apel.

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There is not much scientific evidence supporting the effectiveness of apple cider vinegar. However, its use as a weight loss agent has been increasingly widespread. In fact, in various advertising sales of products, food supplements appear as if it has benefits such as drugs. For that reason, researchers conducted experimental research and hypotheses to be tested is not as good as the effectiveness of apple cider that has a standard drug in losing weight strain of Sprague Dawley rats. Sprague Dawley rats weighing 190-250 grams are used as samples in this study. There were 24 rats divided into 3 groups, namely the control group (as a negative control), mazindol group (as positive control), and apple vinegar. All of those got kind of the same foods and beverages. Weight and attitude of rats were observed for two weeks. Weight data before and after therapy was taken and analyzed using One Way Anova test hypotheses. Researchers found no change in body weight in all three treatment groups. However, the weight of rats continued to increase from day to day. Weight gain data showed a statistically significant difference between control group and apple cider vinegar group (p = 0.012). Meanwhile, there is no significant differences either between control group and mazindol group or between mazindol group and apple cider vinegar group."

"Latar Belakang: American Association of Endodontist (AAE) (2021) menganjurkan penggunaan irigasi NaOCl 1,5% - 3% dan EDTA 17% pada prosedur regeneratif endodontik. Namun, kedua larutan irigasi tersebut memiliki efek terhadap sel punca dan jaringan dentin. Oleh karena itu bahan pengganti bersumber dari bahan alami mulai diteliti, salah satunya adalah cuka apel. Tujuan: Menganalis efek larutan cuka apel berbagai konsentrasi dan larutan irigasi sintetik terhadap topografi dan kekasaran permukaan dentin saluran akar. Metode: 28 spesimen dentin setebal 1 mm dibagi random pada 7 kelompok, lalu tiap kelompok terdiri dari 4 spesimen. Spesimen dentin direndam dengan aquades, NaOCl 1,5%, NaOCl 2,5%, EDTA 17%, larutan cuka apel 2,5%, 5% dan 10% dengan volume 1,8 ml selama 1 menit. Pengamatan topografi dan analisis kekasaran permukaan dentin saluran akar dilakukan dengan mikroskop digital. Hasil: Terdapat efek larutan cuka apel berbagai konsentrasi dan larutan irigasi sintetik terhadap topografi dan kekasaran permukaan dentin saluran akar yang dievaluasi dengan mikroskop digital. Namun tidak terdapat perbedaan efek larutan cuka apel konsentrasi 2,5%, 5% dan 10% dibandingkan dengan NaOCl 1,5%, 2,5% dan EDTA 17% terhadap kekasaran permukaan dentin saluran akar. Kesimpulan: Larutan cuka apel memiliki efek terhadap kekasaran permukaan dentin saluran akar. Nilai rerata kekasaran permukaan terendah adalah cuka apel 2,5% walaupun nilainya tidak berbeda bermakna dengan larutan uji lainnya.

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Background: The American Association of Endodontist (AAE) (2021) recommends the use of 1.5% - 3% NaOCl irrigation and 17% EDTA in regenerative endodontic procedures. However, these two irrigant solutions had an effect on stem cells and dentine tissue. Therefore, substitutes derived from natural ingredients have begun to be researched, one of which is apple vinegar. Objective: To analyze the effect of various concentrations of apple vinegar and synthetic irrigation solutions on topography and surface roughness of root canal dentin Method: 28 specimens of 1 mm thick dentin were randomly devided into 7 groups, then each group consisted of 4 specimens. Dentin specimens were immersed in distilled water, 1.5% NaOCl, 2.5% NaOCl, 17% EDTA, 2.5%, 5% and 10% apple vinegar solution with 1.8 ml volume of solution for 1 minute. The topography and surface roughness observation was carried out using digital microscope. Results: There was effect of apple vinegar solutions with various concentrations and synthetic irrigant solutions on topography and surface roughness of root canal dentin evaluated by digital microscopy. However, there was no difference in the effect of apple vinegar solution concentrations of 2.5%, 5% and 10% compared to 1.5%, 2.5% NaOCl and 17% EDTA on surface roughness of root canal dentin. Conclusion: Apple vinegar solution has an effect on surface roughness of root canal dentin. The lowest average surface roughness value was 2.5% apple vinegar, although the value was not significantly different from the other test solutions."

"Cuka apel merupakan salah satu suplemen makanan yang diklaim dapat digunakan untuk menurunkan berat badan oleh masyarakat Indonesia. Namun, jumlah bukti ilmiah yang mendukung pendapat tersebut masih sedikit. Oleh karena itu, peneliti mengadakan sebuah penelitian dengan metode eksperimental dan berhipotesis bahwa cuka apel dapat menurunkan berat badan tikus strain Sprague Dawley. Dalam penelitian ini digunakan 24 ekor Sprague Dawley dengan berat 190-250 gram sebagai hewan coba. Tikus-tikus ini dibagi menjadi 3 kelompok, yaitu kelompok kontrol (sebagai kontrol negatif), kelompok dietilpropion (sebagai kontrol positif), dan kelompok cuka apel. Ketiga kelompok perlakuan diberi makanan dan minuman dengan jumlah dan jenis yang sama. Selama dua minggu penelitian, dari hari ke hari berat badan dan tingkah laku tikus diamati. Data berat badan sebelum dan sesudah terapi dianalisis secara statistik. Pada akhir penelitian, peneliti tidak menemukan adanya penurunan berat badan pada ketiga kelompok perlakuan, akan tetapi efek sebaliknya terjadi peningkatan berat badan tikus. Hasil analisis statistik menunjukkan adanya perbedaan bermakna antara kelompok kontrol dan kelompok cuka apel (p = 0.012 ). Namun, antara kelompok dietilpropion dan cuka apel tidak ditemukan perbedaan yang bermakna. Hal ini juga ditemukan antara kelompok dietilpropion dan cuka apel.

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Apple cider vinegar is one of food supplements that commonly used to reduce body-weight by Indonesian citizens. But, there is only few scientific proof that supports this opinion. Therefore, researcher held an experimental study and hipotized that apple cider vinegar could reduce the body weight of Sprague Dawley rats. In this study, 24 rats with body weight 190-250 gram are used as experimental animal. These mice were divided into 3 groups, control group (as negative control), diethylpropion group (as positive control group), and apple cider vinegar group. These 3 groups were given the same amount and type of food and drink. For 2 weeks, the body weight and the attitude of mice were observed day by day. The data of mice?s body weight before-and-after treatment were analyzed statistically using SPSS program. In the end of the study, researcher didn?t find any body-weight loss on the 3 groups, but the result of statistical analytic showed that there was significant difference between control group and apple cider vinegar group (p = 0.012). In the other hand, no significant difference found between diethylpropion group and apple cider vinegar group, as well as between dietyhlpropion group and apple cider vinegar group."

"Latar Belakang: Radikal bebas memiliki sifat destruktif pada perawatan kedokteran gigi, salah satunya pada prosedur perawatan saluran akar. Pada perawatan saluran akar, bahan irigasi yang selama ini digunakan dapat menyebabkan kerusakan pada saluran akar karena memiliki senyawa radikal bebas. Oleh karena itu, perlu ditemukan bahan alami herbal yang dapat menjadi sumber antioksidan sebagai penangkal radikal bebas yang dapat berperan positif dalam perawatan saluran akar. Penelitian sudah banyak dikembangkan oleh para peneliti untuk menjadikan larutan ekstrak daun jeruk purut dan larutan cuka apel Malang sebagai obat herbal terstandar dari Indonesia hingga fitofarmaka. Salah satu pengujian yang harus dilakukan adalah pengujian antioksidan karena pada kedua bahan alami herbal tersebut ditemukan senyawa antioksidan berupa fenol dan flavonoid pada uji fitokimia dan GCMS. Tujuan: Penelitian ini bertujuan untuk melihat perbedaan aktivitas dan kadar antioksidan pada larutan ekstrak daun jeruk purut dan larutan cuka apel Malang pada berbagai konsentrasi. Metode: Penelitian ini merupakan eksperimental laboratorik dengan metode DPPH. Sampel uji berupa larutan 10%, 5%, dan 2,5% ekstrak daun jeruk purut dan cuka apel Malang yang dibuat serial konsentrasi dengan pengenceran. Uji aktivitas antioksidan dilakukan dengan melihat perubahan warna pada larutan, dari warna ungu menjadi warna kuning apabila terdapat aktivitas antioksidan di dalam larutan uji. Selanjutnya dilakukan pengukuran kadar antioksidan dengan melihat tingkat absorbansi menggunakan Spektrofotometer UV/VIS dengan panjang gelombang 517 nm dan kemudian dilakukan pengulangan sebanyak 3 kali. Setelah itu, dilakukan perhitungan persentase inhibisi dan didapatkan kadar IC50 antioksidan. Kemudian, perbedaan kadar antioksidan keduanya dilakukan uji statistik Independent T-Test. Hasil: Terdapat aktivitas antioksidan pada larutan ekstrak daun jeruk purut dan larutan cuka apel Malang. Larutan ekstrak daun jeruk purut dapat dideteksi dengan larutan induk konsentrasi 0,1% dengan kadar antioksidan 347,691 µg/ml dan larutan cuka apel Malang dapat dideteksi dengan larutan induk 5% dan 2,5% dengan kadar antioksidan 8375,25 µg/ml dan 8021,162 µg/ml. Keduanya memiliki perbedaan kadar antioksidan secara statistik. Kesimpulan: Terdapat perbedaan kadar antioksidan pada larutan ekstrak daun jeruk purut dan larutan cuka apel Malang dengan berbagai konsentrasi. Larutan ekstrak daun jeruk purut efektif menangkal radikal bebas pada konsentrasi sekitar 0,03%, sedangkan larutan cuka apel Malang efektif pada konsentrasi sekitar 0,8%.

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Background: Free radicals have destructive properties in dental treatments, one of which is in root canal procedures. In root canal treatment, irrigation materials that have been used can cause damage to the root canal because they have free radical compounds. Therefore, it is necessary to find natural herbal ingredients that can be a source of antioxidants as an antidote to free radicals that can play a positive role in root canal treatment. Many studies have been developed by researchers to make kaffir lime leaf extract solution and Malang apple vinegar solution as standardized herbal medicines from Indonesia to phytopharmaceuticals. One of the tests that must be done is antioxidant testing because the two natural herbal ingredients are found antioxidant compounds in the form of phenols and flavonoids in phytochemical and GCMS tests. Objective: This study aims to see the differences in antioxidant activity and levels in kaffir lime leaf extract solution and Malang apple vinegar solution at various concentrations. Methods: This research is a laboratory experiment with DPPH method. Test samples in the form of 10%, 5%, and 2.5% kaffir lime leaf extract solution and Malang apple vinegar solution were made in serial concentrations by dilution. Antioxidant activity test was conducted by looking at the color change in the solution, from purple to yellow color if there is antioxidant activity in the test solution. Furthermore, the antioxidant content was measured by looking at the absorbance level using UV/VIS Spectrophotometer with a wavelength of 517 nm and then repeated 3 times. After that, the percentage of inhibition was calculated and the IC50 value of antioxidant was obtained. Then, the difference in antioxidant levels between the two was carried out Independent T-Test statistical test. Results: There is antioxidant activity in kaffir lime leaf extract solution and Malang apple vinegar solution. Kaffir lime leaf extract solution can be detected with 0.1% concentration mother liquor with antioxidant levels of 347.691 µg/ml and Malang apple vinegar solution can be detected with 5% and 2.5% mother liquor with antioxidant levels of 8375.25 µg/ml and 8021.162 µg/ml. Both have statistically different antioxidant levels. Conclusion: There are differences in antioxidant levels in kaffir lime leaf extract solution and Malang apple vinegar solution with various concentrations. Kaffir lime leaf extract solution is effective against free radicals at a concentration of about 0.03%, while Malang apple vinegar solution is effective at a concentration of about 0.8%."

"Latar belakang: Fokus desinfeksi saluran akar saat ini telah mengalami perubahan dari desinfeksi agresif menjadi proteksi selektif, yang memiliki tujuan utama untuk menciptakan lingkungan yang sesuai, oleh karena itu, bahan irigasi alami sebagai pendamping mulai banyak diteliti potensialnya karena relatif aman. Larutan irigasi dengan kualitas optimal yang dapat membersihkan saluran akar secara menyeluruh sendiri masih belum tersedia dan meskipun ada perkembangan baru di bidang penelitian yang relevan, solusi yang lebih mendekati kualitas ideal belum dikembangkan. Cuka apel dapat dijadikan sebagai bahan alternatif alami yang aman untuk mengeliminasi biofilm sekaligus smear layer, sehingga berpotensi untuk dikembangkan lebih lanjut. Tujuan: Mengetahui perbedaan efek antibakteri bahan irigasi sintetik NaOCl 1,5% dan 2,5% dibandingkan dengan larutan cuka apel terhadap E. faecalis. Metode: Biofilm E. faecalis isolat klinis dibagi menjadi tujuh kelompok sampel untuk dipaparkan dengan bahan uji larutan cuka apel konsentrasi 2,5%, 5%, 10%, kontrol positif NaOCl 1,5%, 2,5% dan kontrol negatif tanpa perlakuan, efek antibakteri dilihat menggunakan metode MTT Assay dan hitung koloni. Hasil: Didapatkan hasil dari kedua uji yang dilakukan bahwa larutan cuka apel konsentrasi 2,5% memiliki efek antibakteri terhadap biofilm E. faecalis tertinggi dibandingkan dengan konsentrasi 5% dan 10%. Kesimpulan: Efek antibakteri larutan cuka apel 2,5%, 5%, dan 10% lebih rendah dibandingkan dengan larutan NaOCl 1,5% dan 2,5% terhadap biofilm E. faecalis.

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Introduction: The current focus of root canal disinfection has changed from aggressive disinfection to selective protection, which has the main goal of creating a suitable environment, therefore, natural irrigation materials as a companion have begun to be studied for their potential because they are relatively safe. Irrigation solutions of optimal quality that can thoroughly clean root canals on their own are not yet available and despite new developments in the relevant research area, solutions closer to the ideal quality have not yet been developed. Apple cider vinegar can be used as a safe natural alternative for eliminating biofilm as well as the smear layer, so it has the potential to be developed further. Objective: To determine the differences in the antibacterial effect of synthetic irrigants NaOCl 1,5% and 2,5% compared to apple cider vinegar solution against E. faecalis. Methods: Clinical isolates of E. faecalis biofilm were divided into seven sample groups to be exposed to test materials for apple cider vinegar concentrations of 2,5%, 5%, 10%, positive control NaOCl 1,5%, 2,5%, and negative control without treatment, the antibacterial effect was seen using the MTT Assay method and colony count. Results: The results of the two tests were obtained that a 2.5% concentration of apple cider vinegar had the highest antibacterial effect on E. faecalis biofilm compared to 5% and 10% concentrations. Conclusion: The antibacterial effect of 2,5%, 5%, and 10% apple cider vinegar solutions was lower than 1,5% and 2,5% NaOCl solutions on E. faecalis biofilm.

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"ABSTRAKMakanlah apel setiap hari dan tubuh Anda akan terhindar dari serangan penyakit. Demikian peribahasa Inggris "An Apple a Day Keeps The Doctor Away". Peribahasa ini tidaklah berlebihan, mengingat beragam manfaat kesehatan yang bisa diperoleh dari apel.Apel fuji dipilih untuk di jadikan sumber baru dalam pembuatan cuka apel karena apel fuji sudah dikenal oleh masyarakat Indonesia maka selebihnya masyarakat juga pasti ingin tahu apa itu apel fuji hasil dari fermentasi (cuka apel fuji). Sehingga penelitian ini mengacu pada bagaimana membuat cuka apel yang layak dikonsumsi masyarakat Indonesia. Alasan inilah yang melatarbelakangi pembuatan cuka apel sebab dijelaskan.Metode pembuatan cuka apel yang dilakukan adalah metode fermentasi alkohol dengan menggunakan saccharomyses cerevisiae dan metode pengasaman dengan menggunakan acetobacter aceti. Metode yang digunakan dalam menguji produk cuka apel adalah metode titrasi, metode penentuan tingkat keasaman, metode penentuan spesifik grafity, metode penentuan kadar alkohol dengan menggunakan GC (fasa produk cair) dan metode penentuan warna, bau dan rasa (Uji Organoleptik).Berbagai variasi yang akan dilakukan dalam penelitian ini adalah variasi penambahan gula; dan variasi penggunaan kultur saccaromyses cereviseae. Hasil yang akan dianalisa adalah kadar alkohol, tingkat keasaman (pH), spesifik grafity, dan kadar asam asetat. Hasil utama yang diinginkan penelitian ini adalah hasil produk cuka apel yang memiliki jumlah alkohol yang sangat sedikit hingga bisa di konsumsi umat muslim pada umumnya dan mempunya rasa sedikit asam, bau cuka yang khas dan warna agak bening. Dari penelitian ini, diharapkan dapat diketahui suatu kondisi optimum dari pembuatan cuka apel hingga menghasilkan cuka apel yang memiliki kadar alkohol rendah dan kadar asam asetat yang layak di konsumsi masyarakat sebagai minuman penyegar.

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ABSTRACTEats apple every day and your body will be protected from disease attack. Said British proverb " An Apple a Day Keeps The Doctor Away". This non abundant proverb, remembers having immeasurable health benefit which able to be obtained from apple.Apple fuji selected for in making new source in making of apple vinegar because apple fuji have been recognized by Indonesia public hence public rest also surely liked to know is that apple fuji result from fermentation ( apple vinegar fuji). So this research refers to how making apple vinegar that is it is good to is consumed Indonesia public. This reason surrounds making of apple vinegar because explained.Making method of apple vinegar done is alcoholic fermentation method by using saccharomyses cerevisiae and acidification method by using acetobacter aceti. Method applied in testing apple vinegar product is titration method, determination method of level of acidity, specific determination method of grafity, determination method of alcohol rate by using GC ( liquid product phase) and determination method of colour, aroma and taste ( Organoleptic Test).Various variation which will be done in this research is various addition of sugar; and various usage of culture saccaromyses cereviseae. Result which will be analysed is alcohol rate, level of acidity ( hydrogen ion exponent), specific of grafity, and acetate acid contents. Main result wanted by this research is result of apple vinegar product having number of a real alcohols is rather finite can in consuming moslem believer in general and its(the pu taste is rather acids, vinegar aroma that is typical and rather colour transparent. From this research, expected is knowable an optimum condition from making of apple vinegar so yielding apple vinegar having low alcohol rate and competent acetate acid contents in consuming public as pickmeup."

"ABSTRAK Pendahuluan. Kesulitan dalam tatalaksana defek tulang yang luas merupakan salah satu tantangan dewasa ini. Selain tatalaksananya yang kompleks juga dapat memberikan dampak jangka panjang negatif yang berat. Penggunaan BMP-2 dalam tatalaksana fraktur dengan defek tulang yang luas memegang peranan penting. BMP-2 berperan pada proses osteogenesis dan chondrogenesis dan menghambat osteoclastogenesis melalui RANKL signaling. Penelitian ini bertujuan untuk mengetahui efek dari perbedaan dosis BMP-2 terhadap penyembuhan fraktur dengan defek tulang yang luas. Metode. Penelitian dilakukan di Laboratorium Animal Gizi di FKUI dan Laboratorium Patologi Anatomi FKUI-RSCM, pada bulan Juli hingga September 2015. Desain penelitian adalah randomized post test control group. Sejumlah 25 ekor tikus putih Sprague Dawley dengan usia 3-4 bulan dan berat badan antara 250 ? 350 gram, dibagi secara acak menjadi kelompok kontrol hidroksiapatit (HA) saja dan kelompok kombinasi HA + BMP-2 1 μg/ml, HA + BMP-2 5 μg/ml, HA + BMP-2 10 μg/ml, HA + BMP-2 20 µg/ml. Tiap kelompok dilakukan tindakan berupa frakturisasi dengan defek tulang 10mm pada femur kanan dan dilakukan fiksasi interna dengan menggunakan intramedullary k-wire ukuran 1,4 mm secara retrograd. Setelah 6 minggu dilakukan penilaian secara histomorfometri, radiologis dan Scanning Electron Microscope (SEM). Hasil. Berdasarkan hasil penelitian secara histomorfometri ditemukan terdapat perbedaan rerata total area kalus yang bermakna diantara kelompok penelitian (p<0,001),terdapat perbedaan bermakna rerata area penulangan antara kelompok kontrol dengan kelompok 1 μg/ml, 5 μg/ml, 10 μg/ml, 20 μg/ml (masing-masing p=0,009, p=0,016, p=0,009 dan p=0,016), terdapat perbedaan bermakna rerata area kartilago antara kelompok kontrol dengan kelompok 1 μg/ml, 5 μg/ml, 10 μg/ml, 20 μg/ml (masing-masing p=0,009, p=0,009, p=0,009 dan p=0,028), terdapat perbedaan bermakna rerata area fibrosis antara kelompok kontrol dengan kelompok 1 μg/ml dengan kelompok kontrol dan 10 μg/ml(masing-masing p=0,047 dan p=0,009).Secara radiologis dengan RUST score didapatkan perbedaan bermakna antara kelompok kontrol dengan kelompok 1 μg/ml, 5 μg/ml, 10 μg/ml, 20 μg/ml (masing-masing p=0,005, p=0,006, p=0,005 dan p=0,006). Dengan SEM didapatkan gambaran kalus yang lebih homogen dan padat pada kelompok 10μg/ml dibandingkan dengan 5 μg/ml dan 20 μg/ml. Kesimpulan: Pemberian BMP-2 dapat menstimulasi proses penyembuhan fraktur pada defek tulang luas (critical bone defect) yang bermakna secara statistik, histomorfometri, radiologis maupun secara kualitatif dengan SEM. Terdapat dosis optimal dalam pemberian BMP-2.ABSTRACT Introduction: Difficulties in the management of extensive bone defects is one of today's challenges. It is not only complex treatment but also can provide long-term negative severe effects. The use of BMP-2 in the treatment of fractures with extensive bone defect plays an important role. BMP-2 plays a role in the process of osteogenesis and chondrogenesis and inhibits osteoclastogenesis via the RANKL signaling. This study aims to determine the effect of differences in doses of BMP-2 on the healing of the fracture with extensive bone defects. Methods: The study was conducted at the Laboratory of Animal Nutrition at the Faculty of Medicine University of Indonesia (FMUI) in July until September 2015. The study design was randomized posttest control group. A number of 25 Sprague Dawley rats aged 3-4 months and bodyweight between 250-350 grams, were randomly divided into a control group of hydroxyapatite (HA) alone and HA+BMP-2 1 µg / ml, HA+BMP -2 5 ug / ml, HA + BMP-2 10 µg / ml, HA + BMP-2 20 ug / ml. Each group carried out fracturization with 10mm bone defect in right femur and internal fixation by using intramedullary K-wire size of 1.4 mm retrograde. After 6 weeks we did histomorfometri assessment, radiological and Scanning Electron Microscope (SEM). Results: Based on the research results histomorfometrcally found there are differences in the mean total area of ​​callus significantly between the study group (p <0.001), there were significant differences in the mean area of ​​woven bone between the control group with group 1 ug / ml, 5 µg / ml, 10 µg / ml, 20 ug / ml (respectively p = 0.009, p = 0.016, p = 0.009 and p = 0.016), there were significant differences in the average area of ​​the cartilage between the control group with group 1 ug / ml, 5 µg / ml, 10 µg / ml, 20 ug / ml (respectively p = 0.009, p = 0.009, p = 0.009 and p = 0.028), there were significant differences in the average area of ​​fibrosis between the control group with group 1 ug / ml in the control group and 10 mg / ml (respectively -masing p = 0.047 and p = 0.009) .In radiologist assessment with RUST scores obtained significant differences between the control group and group 1 ug / ml, 5 µg / ml, 10 µg / ml, 20 µg / ml (respectively p = 0.005 , p = 0.006, p = 0.005 and p = 0.006). SEM features with callus more homogeneous and dense in the group of 10μg / mL compared with 5 ug / ml and 20 µg / ml. Conclusion: Administration of BMP-2 could stimulate the process of fracture healing in large bone defects (critical bone defect) which was statistically significant with histomorfometri assestment, radiological and qualitatively with the SEM. There is an optimal dose in the administration of BMP-2.;Introduction: Difficulties in the management of extensive bone defects is one of today's challenges. It is not only complex treatment but also can provide long-term negative severe effects. The use of BMP-2 in the treatment of fractures with extensive bone defect plays an important role. BMP-2 plays a role in the process of osteogenesis and chondrogenesis and inhibits osteoclastogenesis via the RANKL signaling. This study aims to determine the effect of differences in doses of BMP-2 on the healing of the fracture with extensive bone defects. Methods: The study was conducted at the Laboratory of Animal Nutrition at the Faculty of Medicine University of Indonesia (FMUI) in July until September 2015. The study design was randomized posttest control group. A number of 25 Sprague Dawley rats aged 3-4 months and bodyweight between 250-350 grams, were randomly divided into a control group of hydroxyapatite (HA) alone and HA+BMP-2 1 µg / ml, HA+BMP -2 5 ug / ml, HA + BMP-2 10 µg / ml, HA + BMP-2 20 ug / ml. Each group carried out fracturization with 10mm bone defect in right femur and internal fixation by using intramedullary K-wire size of 1.4 mm retrograde. After 6 weeks we did histomorfometri assessment, radiological and Scanning Electron Microscope (SEM). Results: Based on the research results histomorfometrcally found there are differences in the mean total area of ​​callus significantly between the study group (p <0.001), there were significant differences in the mean area of ​​woven bone between the control group with group 1 ug / ml, 5 µg / ml, 10 µg / ml, 20 ug / ml (respectively p = 0.009, p = 0.016, p = 0.009 and p = 0.016), there were significant differences in the average area of ​​the cartilage between the control group with group 1 ug / ml, 5 µg / ml, 10 µg / ml, 20 ug / ml (respectively p = 0.009, p = 0.009, p = 0.009 and p = 0.028), there were significant differences in the average area of ​​fibrosis between the control group with group 1 ug / ml in the control group and 10 mg / ml (respectively -masing p = 0.047 and p = 0.009) .In radiologist assessment with RUST scores obtained significant differences between the control group and group 1 ug / ml, 5 µg / ml, 10 µg / ml, 20 µg / ml (respectively p = 0.005 , p = 0.006, p = 0.005 and p = 0.006). SEM features with callus more homogeneous and dense in the group of 10μg / mL compared with 5 ug / ml and 20 µg / ml. Conclusion: Administration of BMP-2 could stimulate the process of fracture healing in large bone defects (critical bone defect) which was statistically significant with histomorfometri assestment, radiological and qualitatively with the SEM. There is an optimal dose in the administration of BMP-2."

"Latar Belakang: Fokus disinfeksi saluran akar telah berubah dari disinfeksi agresif menjadi seleksi protektif dalam prosedur regeneratif endodontik. Larutan irigasi sintetik yang digunakan hingga saat ini toksik terhadap sel punca pulpa, salah satunya yang memiliki kemampuan proliferasi dan transdiferensiasi tinggi adalah hDPSCs. Oleh sebab itu, penelitian terkait disinfeksi berbahan alami yang mampu mempertahankan viabilitas sel punca terus berkembang pesat. Salah satu larutan irigasi alami yang bersifat antimikrobial dan agen kelator adalah larutan cuka apel. Untuk menjadikannya obat herbal terstandar hingga fitofarmaka, perlu diidentifikasi kelompok senyawa kimia dan uji viabilitas hDPSCs.Tujuan: Menganalisis pengaruh larutan cuka apel berbagai konsentrasi terhadap viabilitas hDPSCsMetode: hDPSCs ditambahkan DMEM+FBS10% (kontrol negatif), EDTA 17% (kontrol positif), larutan cuka apel dengan konsentrasi 2,5%, 5%, dan 10% dengan enam kali pengulangan. Selanjutnya, persentase viabilitas hDPSCs didapat dari MTT assays melalui microplate reader dalam nilai absorbansi. Data kemudian diolah statistik melalui uji parametrik One-way ANOVA.Hasil: Nilai rerata viabilitas sel hDPSC pada semua kelompok perlakuan bernilai diatas 70% sehingga tidak toksik menurut standar ISO dengan rerata viabilitas tertinggi pada kelompok 2,5% dan terendah pada kelompok EDTA 17% diikuti kelompok 10%.Kesimpulan: Larutan cuka apel dapat diidentifikasi kelompok senyawa kimia dan nilai viabilitas sel paling tinggi pada konsentrasi 2,5%.

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Background: Focus on root canals disinfection have shift from aggressive to protective selection in regenerative endodontic procedures. Synthetic root canals irrigation that had been used until now are toxic toward pulp stem cells, one of them, hDPSCs which have higher proliferation and transdifferentiation ability. Therefore, research on natural disinfection which maintain stem cell viability keep developing rapidly. One of the natural disinfection that has antimicrobial effect and chelating agent is apple cider vinegar. To standardized it as modern medicine, need to identify group of chemical compounds and analyzing the viability percentage of hDPSCs.

Objective: Analyze the impact of apple cider vinegar solution in various concentrations on viability of hDPSCs.

Methods: hDPSCs were given DMEM+FBS10% (negative control), 17% EDTA (positive control), apple cider vinegar solution in 2.5%, 5% and 10% concentrations with six repetitions. Percentage viability of hDPSCs were analyze from MTT assays with microplate reader in absorbance value. Then, data were proccessed statictically with parametric One-way ANOVA.

Results: The average viability of hDPSCs were above 70% which considered non-toxic according to ISO, with the highest cells viability in 2.5% and the lowest cells viability in 17% EDTA followed by 10% groups.

Conclusion: Apple cider vinegar solution’s chemical compounds can be identified with the highest cells viability were at 2.5%."

"ABSTRAKKelapa termasuk produk perkebunan Indonesia yang cukup penting. Pada umumnya bagian kelapa yang dimanfaatkan hanyalah daging buahnya (endosperm), sedangkan bagian lainnya, termasuk air kelapa biasanya terbuang begitu saja. Air kelapa telah terbukti mengandung unsur kimia yang penting bagi pertumbuhan mikroorganisme. Air kelapa dapat dimanfaatkan untuk produksi protein sel tunggal (PST), sari kelapa (nata de coco), atau asam cuka (vinegar). Dalam penelitian ini dibandingkan produk asam cuka yang dihasilkan dari fermentasi air kelapa dengan menggunakan Acetobacter aceti dan A. rancens pada proses asetifikasi dan khamir Saccharomyces cerevisiae var. ellipsoideus pada proses alkoholisasi. Hasil penelitian ini membuktikan bahwa asam cuka yang dihasilkan A. aceti (3,25 %) dan A. rancens (3,48 %) tidak berbeda nyata pada db = 0,05 dengan uji anava l faktor. Hasil uji pengaruh suhu serta uji cita rasa yang dilakukan oleh 2 orang membuktikan bahwa A. rancens lebih tahan suhu tinggi (40 C) serta mempunyai aroma dan rasa asam cuka yang relatif lebih baik dibandingkan asam cuka yang dihasilkan A. aceti. Uji sifat biokimia menyimpulkan bahwa A. aceti dan A. rancens memang berbeda dan harus dipisahkan. Dari hasil penelitian ini, dapat disimpulkan bahwa pemanfaatan air kelapa untuk fermentsi asam cuka, sebaiknya menggunakan A. rancens sebagai inokulumnya."