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Wachid Putranto
Efek kombinasi lisinopril dan diltiazem sebagai antifibrosis pada peritoneum Tikus = Effect of combination lisinopril and diltiazem as antifibrosis in peritoneum of Rats
"[ABSTRAK
Latar Belakang : Continous ambulatory peritoneal dialysis (CAPD) telah menjadi
alternatif selain hemodialisis untuk pengobatan penyakit ginjal tahap akhir. Fibrosis
peritoneum merupakan penyebab utama terjadinya kerusakan membran peritoneum.
Mekanisme fibrosis peritoneum belum diketahui secara pasti, namun ditengarai
transforming growth factor ? β (TGF ?β) berhubungan erat terhadap terjadinya fibrosis
peritoneum.
Tujuan : Tujuan penelitian ini adalah untuk mengetahui pengaruh kombinasi ACE
inhibitor (ACEI) dan calcium channel Blocker (CCB) terhadap penurunan ekspresi TGF
? β dan fibrosis peritoneum tikus jantan yang telah dilakukan CAPD.
Metode Penelitian : Penelitian eksperimental, post test only control group design. Tiga
puluh tikus Dawley spraque dibagi menjadi lima kelompok yaitu kelompok kontrol
(kelompok 1) dan kelompok perlakuan dengan pemberian masing-masing cairan CAPD
4,25% (kelompok2) lisinopril 1,44 mg oral dan CAPD (kelompok 3) diltiazem CD 6,48
mg oral dan CAPD (kelompok 4) lisinopril 1,44 mg dan diltiazem CD 6,48 mg oral dan
CAPD (kelompok 5). Setelah 4 minggu tikus dikorbankan dengan cara dislokasi cervical
kemudian diperiksa ekspresi TGF ? β dan terjadinya fibrosis pada peritoneum tikus,
selanjutnya dibuat sediaan histopatologi dan diwarnai dengan hematoksilin eosin serta
imunohistokimia menggunakan antihuman TGF-ß.
Hasil : Dua puluh peritoneum tikus berhasil diperiksa. Rerata skor TGF-β kelompok
kontrol 1,8, kelompok CAPD 2, kelompok lisinopril dan CAPD 1,8, kelompok diltiazem
CD dan CAPD 1,8, kelompok lisinopril dan diltiazem CD dan CAPD 1,7 (p=0,959).
Rerata skor fibrosis peritoneum kelompok kontrol 1,1, kelompok CAPD 2,6, kelompok
lisinopril dan CAPD 1,2, kelompok diltiazem CD dan CAPD 1,3, kelompok lisinopril dan
diltiazem CD dan CAPD1,5 (p=0,268)
Simpulan : Kombinasi lisinopril dan diltiazem mempunyai kecenderungan menurunkan
ekspresi TGF ? β lebih baik dibandingkan lisinopril maupun diltiazem yang diberikan
secara terpisah tetapi tidak bermakna secara statistik. Kombinasi lisinopril dan diltiazem
mempunyai kecenderungan mengurangi fibrosis peritoneum tetapi tidak bermakna secara
statistik dan tidak lebih baik dibandingkan lisinopril maupun diltiazem bila diberikan
secara terpisah.
ABSTRACT
Background : Continuous ambulatory peritoneal dialysis (CAPD) has been an
alternative other than hemodialysis for end stage kidney disease treatment.
Peritoneal fibrosis is the most serious cause of the damage in membrane
peritoneum. Mechanism of fibrosis peritoneum is not exactly known yet,
transforming growth factor ? β(TGF ? β) is closely related with the existence of
fibrosis peritoneum.
Purposes : The purpose of this study is to evaluate the effect of combination
between ACE inhibitor (ACEI) dan Calcium channel blocker (CCB) in reducing
expression of TGF ? β and fibrosis peritoneum in a male rat treated with CAPD.
Research Method : Experimental study, post test only control group design.
Thirsty Dawley spraque rats are divided into five groups control group ( Group
1), CAPD liquid 4,25% (group 2), lisinopril 1,44 mg oral and CAPD (group 3)
diltiazem CD 6,48 mg oral and CAPD (group 4) lisinopril 1,44mg + diltiazem CD
6,48 mg oral and CAPD (group 5). After 4 weeks, rats sacrificed. Expression of
TGF ? β and peritoneal fibrosis are conducted by histopatology with hematoxillineosin
staining and immunology with anti human-TGF-β.
Result : Twenty peritoneal of rats can be examined. Mean score TGF-β control
group is 1,8, CAPD group is 2, lisinopril and CAPD group is 1,8,diltiazem CD
and CAPD group is 1,8, lisinopril and diltiazem CD and CAPD group is 1,7
(p=0,959) .Mean score peritoneal fibrosis control group is 1,1, CAPD group is
2,6, lisinopril and CAPD group is 1,2, diltiazem CD and CAPD group is 1,3,
lisinopril and diltiazem CD and CAPD group is 1,5 (p=0,268)
Summary : Combination of lisinopril and diltiazem lower the expression of TGF
? β and fibrosis peritoneum better than lisinopril or diltiazem but statistically not
significant. Combination of lisinopril and diltiazem lower the peritoneal fibrosis
but statistically not significant and it doesn?t better than lisinopril or diltiazem.
Key words: ACE inhibitor, calcium channel blocker, TGF-β, peritoneal fibrosis.;Background : Continuous ambulatory peritoneal dialysis (CAPD) has been an
alternative other than hemodialysis for end stage kidney disease treatment.
Peritoneal fibrosis is the most serious cause of the damage in membrane
peritoneum. Mechanism of fibrosis peritoneum is not exactly known yet,
transforming growth factor ? β(TGF ? β) is closely related with the existence of
fibrosis peritoneum.
Purposes : The purpose of this study is to evaluate the effect of combination
between ACE inhibitor (ACEI) dan Calcium channel blocker (CCB) in reducing
expression of TGF ? β and fibrosis peritoneum in a male rat treated with CAPD.
Research Method : Experimental study, post test only control group design.
Thirsty Dawley spraque rats are divided into five groups control group ( Group
1), CAPD liquid 4,25% (group 2), lisinopril 1,44 mg oral and CAPD (group 3)
diltiazem CD 6,48 mg oral and CAPD (group 4) lisinopril 1,44mg + diltiazem CD
6,48 mg oral and CAPD (group 5). After 4 weeks, rats sacrificed. Expression of
TGF ? β and peritoneal fibrosis are conducted by histopatology with hematoxillineosin
staining and immunology with anti human-TGF-β.
Result : Twenty peritoneal of rats can be examined. Mean score TGF-β control
group is 1,8, CAPD group is 2, lisinopril and CAPD group is 1,8,diltiazem CD
and CAPD group is 1,8, lisinopril and diltiazem CD and CAPD group is 1,7
(p=0,959) .Mean score peritoneal fibrosis control group is 1,1, CAPD group is
2,6, lisinopril and CAPD group is 1,2, diltiazem CD and CAPD group is 1,3,
lisinopril and diltiazem CD and CAPD group is 1,5 (p=0,268)
Summary : Combination of lisinopril and diltiazem lower the expression of TGF
? β and fibrosis peritoneum better than lisinopril or diltiazem but statistically not
significant. Combination of lisinopril and diltiazem lower the peritoneal fibrosis
but statistically not significant and it doesn?t better than lisinopril or diltiazem.
Key words: ACE inhibitor, calcium channel blocker, TGF-β, peritoneal fibrosis.;Background : Continuous ambulatory peritoneal dialysis (CAPD) has been an
alternative other than hemodialysis for end stage kidney disease treatment.
Peritoneal fibrosis is the most serious cause of the damage in membrane
peritoneum. Mechanism of fibrosis peritoneum is not exactly known yet,
transforming growth factor ? β(TGF ? β) is closely related with the existence of
fibrosis peritoneum.
Purposes : The purpose of this study is to evaluate the effect of combination
between ACE inhibitor (ACEI) dan Calcium channel blocker (CCB) in reducing
expression of TGF ? β and fibrosis peritoneum in a male rat treated with CAPD.
Research Method : Experimental study, post test only control group design.
Thirsty Dawley spraque rats are divided into five groups control group ( Group
1), CAPD liquid 4,25% (group 2), lisinopril 1,44 mg oral and CAPD (group 3)
diltiazem CD 6,48 mg oral and CAPD (group 4) lisinopril 1,44mg + diltiazem CD
6,48 mg oral and CAPD (group 5). After 4 weeks, rats sacrificed. Expression of
TGF ? β and peritoneal fibrosis are conducted by histopatology with hematoxillineosin
staining and immunology with anti human-TGF-β.
Result : Twenty peritoneal of rats can be examined. Mean score TGF-β control
group is 1,8, CAPD group is 2, lisinopril and CAPD group is 1,8,diltiazem CD
and CAPD group is 1,8, lisinopril and diltiazem CD and CAPD group is 1,7
(p=0,959) .Mean score peritoneal fibrosis control group is 1,1, CAPD group is
2,6, lisinopril and CAPD group is 1,2, diltiazem CD and CAPD group is 1,3,
lisinopril and diltiazem CD and CAPD group is 1,5 (p=0,268)
Summary : Combination of lisinopril and diltiazem lower the expression of TGF
? β and fibrosis peritoneum better than lisinopril or diltiazem but statistically not
significant. Combination of lisinopril and diltiazem lower the peritoneal fibrosis
but statistically not significant and it doesn?t better than lisinopril or diltiazem.
Key words: ACE inhibitor, calcium channel blocker, TGF-β, peritoneal fibrosis.;Background : Continuous ambulatory peritoneal dialysis (CAPD) has been an
alternative other than hemodialysis for end stage kidney disease treatment.
Peritoneal fibrosis is the most serious cause of the damage in membrane
peritoneum. Mechanism of fibrosis peritoneum is not exactly known yet,
transforming growth factor ? β(TGF ? β) is closely related with the existence of
fibrosis peritoneum.
Purposes : The purpose of this study is to evaluate the effect of combination
between ACE inhibitor (ACEI) dan Calcium channel blocker (CCB) in reducing
expression of TGF ? β and fibrosis peritoneum in a male rat treated with CAPD.
Research Method : Experimental study, post test only control group design.
Thirsty Dawley spraque rats are divided into five groups control group ( Group
1), CAPD liquid 4,25% (group 2), lisinopril 1,44 mg oral and CAPD (group 3)
diltiazem CD 6,48 mg oral and CAPD (group 4) lisinopril 1,44mg + diltiazem CD
6,48 mg oral and CAPD (group 5). After 4 weeks, rats sacrificed. Expression of
TGF ? β and peritoneal fibrosis are conducted by histopatology with hematoxillineosin
staining and immunology with anti human-TGF-β.
Result : Twenty peritoneal of rats can be examined. Mean score TGF-β control
group is 1,8, CAPD group is 2, lisinopril and CAPD group is 1,8,diltiazem CD
and CAPD group is 1,8, lisinopril and diltiazem CD and CAPD group is 1,7
(p=0,959) .Mean score peritoneal fibrosis control group is 1,1, CAPD group is
2,6, lisinopril and CAPD group is 1,2, diltiazem CD and CAPD group is 1,3,
lisinopril and diltiazem CD and CAPD group is 1,5 (p=0,268)
Summary : Combination of lisinopril and diltiazem lower the expression of TGF
? β and fibrosis peritoneum better than lisinopril or diltiazem but statistically not
significant. Combination of lisinopril and diltiazem lower the peritoneal fibrosis
but statistically not significant and it doesn?t better than lisinopril or diltiazem.
Key words: ACE inhibitor, calcium channel blocker, TGF-β, peritoneal fibrosis.;Background : Continuous ambulatory peritoneal dialysis (CAPD) has been an
alternative other than hemodialysis for end stage kidney disease treatment.
Peritoneal fibrosis is the most serious cause of the damage in membrane
peritoneum. Mechanism of fibrosis peritoneum is not exactly known yet,
transforming growth factor ? β(TGF ? β) is closely related with the existence of
fibrosis peritoneum.
Purposes : The purpose of this study is to evaluate the effect of combination
between ACE inhibitor (ACEI) dan Calcium channel blocker (CCB) in reducing
expression of TGF ? β and fibrosis peritoneum in a male rat treated with CAPD.
Research Method : Experimental study, post test only control group design.
Thirsty Dawley spraque rats are divided into five groups control group ( Group
1), CAPD liquid 4,25% (group 2), lisinopril 1,44 mg oral and CAPD (group 3)
diltiazem CD 6,48 mg oral and CAPD (group 4) lisinopril 1,44mg + diltiazem CD
6,48 mg oral and CAPD (group 5). After 4 weeks, rats sacrificed. Expression of
TGF ? β and peritoneal fibrosis are conducted by histopatology with hematoxillineosin
staining and immunology with anti human-TGF-β.
Result : Twenty peritoneal of rats can be examined. Mean score TGF-β control
group is 1,8, CAPD group is 2, lisinopril and CAPD group is 1,8,diltiazem CD
and CAPD group is 1,8, lisinopril and diltiazem CD and CAPD group is 1,7
(p=0,959) .Mean score peritoneal fibrosis control group is 1,1, CAPD group is
2,6, lisinopril and CAPD group is 1,2, diltiazem CD and CAPD group is 1,3,
lisinopril and diltiazem CD and CAPD group is 1,5 (p=0,268)
Summary : Combination of lisinopril and diltiazem lower the expression of TGF
? β and fibrosis peritoneum better than lisinopril or diltiazem but statistically not
significant. Combination of lisinopril and diltiazem lower the peritoneal fibrosis
but statistically not significant and it doesn?t better than lisinopril or diltiazem.
Key words: ACE inhibitor, calcium channel blocker, TGF-β, peritoneal fibrosis., Background : Continuous ambulatory peritoneal dialysis (CAPD) has been an
alternative other than hemodialysis for end stage kidney disease treatment.
Peritoneal fibrosis is the most serious cause of the damage in membrane
peritoneum. Mechanism of fibrosis peritoneum is not exactly known yet,
transforming growth factor – β(TGF – β) is closely related with the existence of
fibrosis peritoneum.
Purposes : The purpose of this study is to evaluate the effect of combination
between ACE inhibitor (ACEI) dan Calcium channel blocker (CCB) in reducing
expression of TGF – β and fibrosis peritoneum in a male rat treated with CAPD.
Research Method : Experimental study, post test only control group design.
Thirsty Dawley spraque rats are divided into five groups control group ( Group
1), CAPD liquid 4,25% (group 2), lisinopril 1,44 mg oral and CAPD (group 3)
diltiazem CD 6,48 mg oral and CAPD (group 4) lisinopril 1,44mg + diltiazem CD
6,48 mg oral and CAPD (group 5). After 4 weeks, rats sacrificed. Expression of
TGF – β and peritoneal fibrosis are conducted by histopatology with hematoxillineosin
staining and immunology with anti human-TGF-β.
Result : Twenty peritoneal of rats can be examined. Mean score TGF-β control
group is 1,8, CAPD group is 2, lisinopril and CAPD group is 1,8,diltiazem CD
and CAPD group is 1,8, lisinopril and diltiazem CD and CAPD group is 1,7
(p=0,959) .Mean score peritoneal fibrosis control group is 1,1, CAPD group is
2,6, lisinopril and CAPD group is 1,2, diltiazem CD and CAPD group is 1,3,
lisinopril and diltiazem CD and CAPD group is 1,5 (p=0,268)
Summary : Combination of lisinopril and diltiazem lower the expression of TGF
– β and fibrosis peritoneum better than lisinopril or diltiazem but statistically not
significant. Combination of lisinopril and diltiazem lower the peritoneal fibrosis
but statistically not significant and it doesn’t better than lisinopril or diltiazem.
Key words: ACE inhibitor, calcium channel blocker, TGF-β, peritoneal fibrosis.]"
2016
T-Pdf
UI - Tesis Membership  Universitas Indonesia Library
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Yenny
Analisis peran karakteristik peritoneum dan konsentrasi glukosa cairan capd terhadap pengeluaran cairan pada pasien yang menjalani capd di RS PGI Cikini Jakarta
"ABSTRAK
Karakteristik peritoneum dan konsentrasi glukosa mempengaruhi perbedaan tekanan osmotik sepanjang dwell time pada CAPD. Penelitian ini bertujuan menganalisis peran karakteristik peritoneum dan konsentrasi glukosa terhadap pengeluaran cairan. Desain penelitian cross-sectional dengan pendekatan retrospektif. Berdasarkan consecutive sampling didapat 53 responden. Data berasal dari tahun 2005-2010. Hasil uji t menunjukkan nilai p<0,05 pada rerata pengeluaran cairan berdasarkan konsentrai glukosa pada siang dan malam. Hasil uji ANOVA menunjukkan nilai p<0,05 pada rerata pengeluaran cairan berdasarkan karakteristik peritoneum pada pagi dan sore. Peneliti menyimpulkan karakteristik peritoneum dan konsentrasi glukosa berperan terhadap pengeluaran cairan pada CSPD."
2010
T28422
UI - Tesis Open  Universitas Indonesia Library
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Ernawati Munir
Aktivitas antifibrosis ekstrak turbinaria decuren terkarakterisasi melalui penghambatan TGF1, smad3 dan mmp13 serta pengaruh terhadap aktivitas decorin pada hati tikus yang diinduksi ccl4 = Antifibrotic activities of turbinaria decuren extract characterized by inhibiting tgf 1 smad3 mmp13 and the effect on the activity of decorin in rat liver induced ccl
"ABSTRAK
Pendahuluan: Fibrosis hati yang diakibatkan oleh stres oksidatif ROS dan transformasifaktor pertumbuhan beta 1 TGF?-1 menjadi salah satu target dalam pencegahan danpengobatan fibrosis hati. Rumput laut coklat Turbinaria decuren diketahui mengandungfukoidan yang bersifat ionik memiliki bioaktivitas berdasarkan spesies dan berat molekulnamun belum diteliti secara intensif. Penelitian ini dilakukan dengan tujuan membuktikanefek antifibrosis ekstrak T. decuren terkarakterisasi eTkar melalui penghambatan aktivitasTGF-?1, MMP 13 dan Smad3 serta pengaruhnya terhadap decorin.
Metode: Penelitian dilakukan di Labkesda Provinsi DKI Jakarta, Laboratorium HistologiFKUI, Laboratorium Farmakologi FKUI dan Laboratorium Terpadu FKUI. EkstrakT.decuren terkarakterisasi eTkar hasil penelitian tahap I akan diuji efeknya sebagaiantifibrosis melalui mekanisme preventif dan kuratif pada tikus jantan galur SpragueDawley SD yang diinduksi dengan CCl4. 50 dosis 1 ml/100 gram BB dua kali seminggselama 8 minggu. Enam puluh duatikus jantan galur SD dibagi 2 kelompok uji yaitu A: ujipreventif dan B: uji kuratif, masing-masing dibagi menjadi 7 kelompok perlakuan secaraacak. eTkar diberikan pada dosis 27,5, 55, 110 mg/kg BB p.o. Pada uji preventif eTkardiberikan 1 minggu sebelum dan 7 minggu bersamaan pemberian CCl4.. Pada pengujian kuratif diberikan CCl4 selama 2 minggu dan 6 minggu secara bersamaan dengan sampel uji.Pemeriksaan yang dilakukan: ALT, AST, ALP, GGT, MDA, GSH, protein total, TGF?-1,Smad3, MMP 13, decorin dan pemeriksaan histopatologi jaringan hati. Sebagaipembanding positif digunakan fukoidan 50 mg/Kg BB.
Hasil: Pada uji preventif dosis eTkar yang mampu menekan luas fibrosis hati tertinggiadalah 27,5 mg/kg BB sebesar 69,89 lebih tinggi dari fukoidan dan berbeda bermaknadengan kontrol - . Pada dosis ini juga mampu menekan secara bermakna aktivitas TGF?1,MMP 13, ekspresi Smad3, decorin dan meningkatkan kadar GSH secara bermaknadibanding kontrol - , tetapi tidak bermakna untuk MDA. Pada uji kuratif, eTkar dosis 55mg/Kg BB mampu menekan persentase luas fibrosis hati sebesar 62,05 yang berbedabermakna dibanding kontrol - p
ABSTRACT
Introduction. Liver fibrosis due to oxidative stress ROS and transforming growth factor beta 1 TGF 1 became one of the targets in the prevention and treatment of liver fibrosis. The brown seaweed T.decurens fucoidan containing ionic compounds have a potent bioactive potentials, which depends on species and molecular weight. but still unknown mechanism of action in inhibiting liver fibrosis. This study was conducted in order to prove the effect of the extract antifibrosis characterized by constraints on the expression of protein TGF 1, Smad3 and its effect on protein MMP 13 and decorin.
Method: The study was conducted in Labkesda DKI Jakarta province, Histology Laboratory, Pharmacology Laboratory and Integrated Laboratory School of Medicine FKUI. Characterized extracts brown seaweed T.decuren eTkar of the results of the phase I study was studied to investigate their effect as antifibrosis in animal model of fibrosis through preventive and curative mechanism on male rats Sprague Dawley SD induced by CCl by 50 CCl ndash 1 mL kg BW twice a week for 8 weeks p.o. Sixty two male rats SD divided into 2 groups, namely A test test preventive and B test curative. Each divided into 7 treatment groups at random. eTkar was given a three level doses, i.e 27,5, 55, 110 mg kg BW p.o. At preventive mechanism, eTkar was delivered for 1 week before and 7 weeks of concurrent CCl 4 administration. On curative mechanism, eTkar was delivered the last 6 weeks for 8 weeks CCl 4 adminstration simulaneously. The test parameters were biomarkers of liver injury ALT, AST, MDA and GSH liver , liver function ALP, GGT and albumin , 4 the degree of fibrosis fibrosis area, TGF 1 and MMP 13 activities, Smad and decorin expression, fatty area hispatologically . Fucoidan was used as positive control at the dose of 50 mg Kg BW.
Results: In the preventive method, the optimum dose of eTkar capable to suppress liver fibrosis was 27,5 mg kg BW by 69,89 of CCl group which was better than comercial fucoidan and significanly different. At this dose eTkar was able to significanly suppress the 4 activity TGF 1, MMP 13, expression Smad3 decorin and increase GSH level significanly different to group p."
2017
D-Pdf
UI - Disertasi Membership  Universitas Indonesia Library
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Yunita Sari
Analisis Asuhan Keperawatan Pada Pasien Peritonitis TB Disertai Efusi Pleura Dengan Penerapan Pursed Lip Breathing Dan Graded Exercise Therapy di RSUPN Dr.Cipto Mangunkusumo = Analysis of Nursing Care in Peritoneal Tuberculosis Patients Accompanied by Pleural Effusion With the Application of Pursed Lip Breathing and Graded Exercise Therapy at Cipto Mangunkusumo Hospital
"Peritonitis tuberkulosis adalah peradangan peritoneum parietal atau visceral yang disebabkan oleh mycobacterium tuberculosis. Efusi pleura terjadi karena komplikasi dari penyakit yang menyertai maupun proses infeksi yang mengenai rongga pleura. Masalah keperawatan yang dapat ditegakkan yaitu gangguan pertukaran gas dan intoleransi aktivitas. Karya Ilmiah Akhir Ners ini bertujuan untuk menganalisis asuhan keperawatan dengan pemberian intervensi pursed lip breathing dan graded exercise therapy. Metode yang digunakan berupa laporan kasus yang dikelola selama 4 hari berdasarkan tinjauan literatur. Hasil analisis kombinasi intervensi mengenai efektifitas pemberian intervensi pursed lip breathing dan graded exercise therapy terbukti efektif dalam mengatasi masalah gangguan pertukaran gas dan intoleransi aktivitas. Dibuktikan dengan perubahan tanda-tanda vital menjadi lebih stabil, frekuensi pernapasan dan frekuensi nadi membaik, serta keluhan sesak pasien berkurang. Toleransi aktivitas pasien menjadi lebih meningkat dengan kemampuan self care yang meningkat. Pemberian intervensi pursed lip breathing dan graded exercise therapy direkomendasikan dalam mengatasi gangguan pertukaran gas dan intoleransi aktivitas.
Peritoneal tuberculosis is an inflammation of the parietal or visceral peritoneum caused by mycobacterium tuberculosis. Pleural effusion occurs due to complications from the accompanying disease as well as infectious processes that affect the pleural cavity. Enforceable nursing problems are gas exchange disorders and activity intolerance. This study aims to analyze nursing care by providing pursed lip breathing and graded exercise therapy   interventions. The method that used is a case report that is managed for four days based on a literature review. The results of the combination analysis of interventions regarding the effectiveness of giving pursed lip breathing and graded exercise therapy interventions have been shown to be effective in overcoming the problems of gas exchange disorders and activity intolerance. Evidenced by the change of vital signs to become more stable, the frequency of breathing and the frequency of the pulse improve, as well as dyspnea of the patient are reduced. The tolerance of patient activity improved with the increaseasing self-care ability. Intervention of pursed lip breathing and graded exercise therapy is recommended in overcoming gas exchange disorders and activity intolerance."
Depok: Fakultas Ilmu Keperawatan Universitas Indonesia, 2022
PR-pdf
UI - Tugas Akhir  Universitas Indonesia Library
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Endoglin concentration in peritoneal fluid of patients with endometriosis
"Latar belakang: Endoglin adalah faktor angiogenesis spesifik yang diduga terkait dengan patogenesis endometriosis. Tujuan penelitian ini adalah mengevaluasi hubungan kadar endoglin pada cairan peritoneum pasien dengan endometriosis.
Metode: Penelitian ini merupakan penelitian observasional potong lintang yang dilaksanakan pada bulan Maret 2011 sampai Juli 2012 di RS Dr. Wahidin Sudirohusodo dan rumah sakit swasta di Makassar. Semua pasien yang akan menjalani laparoskopi untuk masalah infertilitas dan masalah lainnya serta memenuhi kriteria inklusi diminta untuk mengisi
kuesioner dan diambil cairan peritoneum sebanyak 5 cc untuk mengukur kadar endoglin dengan metode ELISA. Stadium endometriosis ditegakkan dengan memakai kriteria ASRM dan dipisahkan menjadi endometriosis ringan dan berat. Data dianalisis dengan Excel dan analisis korelasi Spearman.
Hasil: Pada kelompok endometriosis didapatkan konsentrasi endoglin antara 14,43-15,65 ng/mL (median 15,04 ng/mL) sedangkan pada kelompok kontrol antara 7,42-10,26 ng/mL (median 8,84 ng/mL). Kadar endoglin peritoneum sama atau lebih tinggi dari 11 ng/mL mungkin dipakai sebagai titik potong prediktor kasus endometriosis.
Kesimpulan: Kadar endoglin peritoneum meningkat secara proporsional sesuai dengan meningkatnya stadium endometriosis, sehingga kadar endoglin dapat dipakai sebagai prediktor endometriosis.
Abstract
Background: Endoglin is a specific angiogenic factor suspected to involve in the pathogenesis of endometriosis. The aim of this study was to evaluate the significance of endoglin concentration in the peritoneal fluid of patients with endometriosis.
Methods: This pilot study was performed between March 2011 and July 2012 at Dr. Wahidin Sudirohusodo Hospital and another private hospital in Makassar, Indonesia. This was an observational, cross-sectional study. All patients undergoing laparoscopy for infertility and other cases with suitable inclusion criteria were asked to answer a questionnaire and had a 5 cc peritoneal fluid sample taken for measurement of peritoneal endoglin concentration using ELISA. Endometriosis stage was classified using ASRM criteria and divided into two groups, mild and severe. All data were analyed using Excel and Spearman correlation analysis.
Results: In the endometriosis group peritoneal endoglin concentration ranged between 14.43- 15.65 ng/mL (median 15.04 ng/mL) which is higher than control group 7.42-10.26 ng/mL (median 8.84 ng/mL). A peritoneal endoglin concentration equal to or higher than 11 ng/mL could be used to predict endometriosis.
Conclusion: Endoglin concentrations increased proportionally with the severity of endometriosis, and many be used as predictive factor of endometriosis cases."
[Fakultas Kedokteran Universitas Indonesia, Universitas Hasanuddin. Fakultas Kedokteran], 2013
pdf
Artikel Jurnal  Universitas Indonesia Library
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Sianturi, Dahlan
Pengaruh kombinasi recombinant humun Hepatocyte Growtth Factor dan vesikel ekstraseluler terhadap fibrosis hati (skor Laennec), penanda cedera hati (AST dan ALT) serta Hepatocyte Growth Factor pada model tikus ligasi duktus bilier = The effect of combination of recombinant human hepatocyte growth factor and extracellular vesicles in liver fibrosis (score Laennec), liver injury markers (AST and ALT), and hepatocyte growth factor un the rat bile duct ligation model
"Latar Belakang: Fibrosis hati disebabkan cedera hati kronis akan menjadi sirosis. Vesikel ekstraseluler (VE) dan Hepatocyte Growth Factor (HGF) banyak dipelajari sebagai terapi fibrosis dan regenerasi hati. Penelitian ini memanfaatkan kombinasi VE dan recombinant human-HGF ( rh-HGF) sebagai terapi alternatif fibrosis hati pada model tikus ligasi duktus bilier (LDB).
Metode: Sebelas tikus Sprague Dawley (SD) menjalani LDB, 5 tikus kontrol dan 6 tikus mendapat perlakuan injeksi VE 150 uL dan rh-HGF 0,1 mg intravena sejak 3 minggu pasca LDB, selama 7 hari. Satu tikus kontrol diterminasi 3 minggu pasca LDB sebagai data dasar. Tikus-tikus kelompok kontrol dan perlakuan diterminasi pada 1 hari dan 2 minggu setelah injeksi terakhir. Dilakukan penilaian skor Laennec hati serta kadar HGF, alanine aminotransferase (ALT) dan aspartate aminotransferase (AST).
Hasil: Histopatologi 3 minggu pasca LDB menunjukkan skor Laennec 2. Skor fibrosis kelompok kontrol (KK) dan kelompok perlakuan (KP) 1 minggu dan 2 minggu pasca injeksi VE dan rh-HGF seluruhnya dengan skor 2. Kadar HGF pada KP lebih rendah secara signifikan dibandingkan dengan KK 1 hari pasca injeksi terakhir (1,35+0,06 vs 1,53+0,06; p<0,001), semakin menurun setelah 2 minggu pasca injeksi terakhir namun tidak bermakna secara statistik 0,83 (0,73-0,84) vs 0,76 (0,73-0,80) p=0,248). Kadar AST 1 hari pasca injeksi terakhir; KK 1,66+0,05 KP 0,91+0,12 debgan p = 0,001. Setelah 2 minggu pasca injeksi; kadar AST pada kedua kelompok menurun 1,12 (1,11-1,22) vs (0,96+0,03); p=0,021). Kadar ALT pada kelompok perlakuan lebih rendah secara signifikan pada 1 hari pasca injeksi dan 2 minggu pasca injeksi dengan nilai p<0,001 dan 0,033a
Background: Fibrosis of the liver due to a chronic liver injury will become cirrhosis at the end-stage. The Extracellular Vesicles (EV) and hepatocyte growth factor (HGF) are recently learned as much as fibrosis and liver regeneration therapy. This study aims to know the result of using a combination of EV and recombinant human HGF (rh-HGF) as an alternative therapy due to liver fibrosis on the rat bile duct ligation (BDL) model.
Methods: Eleven BDL Sprague Dawley (SD) were divided into two groups, five mice as the untreated control group and six mice as the treatment group was getting an EV 150 ul and rh-hgf 0.1 mg intravenous injection three weeks after BDL, for seven days. One control rat is expanded three weeks after BDL as baseline data. The control and treatment group mice were projected at day one and two weeks after the final injection. This study was assessed from liver fibrosis by using Laennec score, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and Hepatocyte Growth Factor (HGF) level.
Results: Scoring of histopathology by using the Laennec score at 3 weeks after BDL was 2. Meanwhile scoring of fibrosis of the control group and treatment group at 1 week and 2 weeks after ve and rh-hgf injection were 2. HGF level of the treatment group was lower significantly than control group at day 1 after last injection (1,35+0,06 vs 1,53+0,06; p<0,001), and within 2 weeks, the number of HGF levels decreased but statistically insignificant; 0,83 (0,73-0,84) vs 0,76 (0,73-0,80) p=0,248). AST level at day 1 after last injection; control group vs treatment group ;1,66+0,05 vs 0,91+0,12, p value = 0,001. 2 weeks after injection; AST level for both group were become lower 1,12 (1,11-1,22) vs (0,96+0,03); p=0,021), and ALT level on treatment group was significantly lower at day 1 and 2 weeks after injection with p value <0,001 and 0, 033a"
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2021
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Perbedaan ekspresi TGF-β1 dan fibrosis intertisial pada kejadian nefrotoksis doxorubicin dan nefroprotektif pentoxifylin
"Nephrotoxic effects of Doxorubicin (DXR) is still a problem in clinical practice. On the other hand Pentoxyfilline (PTX) as an electron-donor material can be nephroprotective. Therefore, combination of DXR and PTX would be expected to reduce nephrotoxic effects of DXR. In this study we examined the effects of PTX on TGF-B1 expression and interstitial fibrosis in an experimental model of DXR nephropathy in mice. Mice were divided into three groups of eight each i.e. untreated Swiss mice (controls), DXR treatment alone to induce nephropathy, and DXR treatment followed by PTX. Following 4 week treatment, each group was sacrificed. Examination of TGF-B1 expression was carried out by immunohistochemistry employing monoclonal antibody. Interstitial fibrosis examination was performed by a histopathologist using Verheoff van Giesen staining and the one way Anova was used for statistical analysis. It was observed that DXR treatment followed by PTX treatment prevented the increase of TGF-B1 expression and interstitial fibrosis in mice with DXRnephropathy (p<0.05). These findings suggested the beneficial nephroprotective effect of PTX."
610 JKY 17: 2 (2009)
Artikel Jurnal  Universitas Indonesia Library
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Nur Azizah
Analisis Histologi dan Ekspresi Gen Matrix Metalloproteinase-2 (MMP-2) Satu Minggu Pascainjeksi Umbilical Cord-Mesenchymal Stem Cell (UC-MSC) pada Fibrosis Hati Kelinci Oryctolagus cuniculus (Linnaeus, 1758) = Histological Analysis and Gene Expression of Matrix Metalloproteinase-2 (MMP-2) One Week Postinjection Umbilical Cord-Mesenchymal Stem Cell (UC-MSC) in Rabbit Oryctolagus Cuniculus (Linnaeus, 1758) Liver Fibrosis
"Fibrosis hati merupakan penyakit dengan tingkat morbiditas dan mortalitas yang tinggi. Terapi yang efektif dalam mengatasi fibrosis hanyalah transplantasi hati, tetapi mahal dan sulit didapatkan sehingga diperlukan alternatif lain. Umbilical cord mesenchymal stem cell (UC-MSC) mampu mendegradasi matriks ekstraselular sehingga potensial mengatasi fibrosis. Tujuan penelitian adalah mengetahui pengaruh UC-MSC terhadap fibrosis pada hati kelinci (Oryctolagus cuniculus). Jumlah sampel yang digunakan sebanyak 16 hati yang terdiri dari 2 kelompok normal, 7 kelompok fibrosis model ligasi duktus bilier (LDB), dan 7 kelompok fibrosis yang diinjeksi UC-MSC secara intrahepatika (LDB + UC-MSC). Penelitian dilakukan dengan analisis histologi dan ekspresi gen Matrix Metalloproteinase-2 (­MMP-2). Sediaan histologi diwarnai dengan pewarnaan Hematoksilin-Eosin untuk analisis sistem penilaian Laennec dan Masson Trichrome untuk analisis area fraksi kolagen. Analisis ekspresi gen MMP-2 dilakukan dengan metode qRT-PCR. Hasil analisis histologi berdasarkan sistem penilaian Laennec antara kelompok LDB dan LDB + UC-MSC tidak terdapat perbedaan, sedangkan persentase area kolagen menunjukkan perbedaan yang signifikan (p < 0,0001). Hasil analisis ekspresi gen MMP-2 pada kedua kelompok menunjukkan perbedaan yang tidak signifikan (p = 0,2593). Dapat disimpulkan bahwa UC-MSC dapat mengurangi area fraksi kolagen dengan kecenderungan peningkatan ekspresi gen MMP-2 walaupun belum terdapat perubahan secara morfologi.
Liver fibrosis is a disease with high morbidity and mortality. The effective therapy is liver transplantation, but it is expensive and difficult, so needs alternative. Umbilical cord mesenchymal stem cell (UC-MSC) can degrade extracellular matrix which is potential to decrease fibrosis. The study aims to know the effect of UC-MSC on liver fibrosis in rabbits (Oryctolagus cuniculus). The sample is 16 livers consisting of 2 normal groups, 7 groups of biliary duct ligation fibrosis models (LDB), and 7 groups of fibrosis injected intrahepatic UC-MSC (LDB + UC-MSC). The study uses histological and matrix metalloproteinase-2 (MMP-2) gene expression analysis. Histological slides were stained by Hematoxylin-Eosin for Laennec scoring system and Masson Trichrome for analyze collagen fractions area. Analysis of MMP-2 gene expression was assessed using qRT-PCR. The results of histological analysis based on the Laennec scoring system showed no difference between the LDB and LDB + UC-MSC groups, while the percentage of collagen area showed a significant difference (p <0.0001). The results of the MMP-2 gene expression in the two groups showed no significant difference (p = 0.2593). The conclusion is UC-MSC can reduce the collagen fraction area with a tendency to increase MMP-2 gene expression although no change in morphology."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2023
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Sisilia Triani Sisilia Triani
Formulasi beads tetrandrine menggunakan metode gelasi ionik ca-pektinat tersalut hidroksipropilmetil selulosa ftalat atau selulosa asetat ftalat sebagai sediaan kolon tertarget = Formulation of tetrandrine beads using ionic gelation method ca pectinate coated hydroxypropylmetyl cellulose phthalate or cellulose acetate phthalate as colon targeted
"Prevalensi kejadian crohn?s disease dan ulcerative colitis di dunia masih terus meningkat. Kedua penyakit ini termasuk dalam inflammatory bowel disease (IBD). Meskipun telah terdapat sejumlah pilihan terapi untuk pasien dengan penyakit ini, namun tindakan pembedahan masih menjadi satu-satunya pilihan untuk mengatasi pembentukan jaringan parut (fibrosis). Tetrandrine dipilih sebagai zat aktif pada penelitian ini karena telah diketahui memiliki efek antifibrosis. Penelitian ini dilakukan untuk mengembangkan dan mengevaluasi sediaan beads kalsium pektinat tetrandrine menggunakan properti yang sensitif pH untuk menarget kolon. Beads dibuat menggunakan metode gelasi ionik dan dilanjutkan dengan proses penyalutan oleh HPMCP HP-55 atau CAP. Beads tidak tersalut dievaluasi ukuran partikel, bentuk, morfologi, kemampuan mengembang, efisiensi proses, dan efisiensi penjerapan. Dari hasil evaluasi ini diputuskan beads dengan konsentrasi larutan kalsium klorida 5% (formula 1) akan digunakan untuk penyalutan. Beads formula 1 memiliki bentuk lebih sferis, tidak terlalu lengket, dan ukuran lebih kecil bila dibandingkan dengan beads konsentrasi kalsium klorida 10% (formula 2) dan 15% (formula 3). Nilai efisiensi penjerapan dari ketiga beads secara berurutan yakni 65,67 ± 0,39%, 68,03 ± 0,12%, 56,28 ± 0,2%. Setelah disalut, beads kemudian digunakan untuk uji pelepasan secara in vitro dan uji pentargetan. Dari hasil pengujian diperoleh beads kalsium pektinat tersalut mampu menahan pelepasan tetrandrine dalam medium asam, namun belum berhasil menarget kolon.
Prevalances of crohn?s disease and ulcerative colitis in the world are still increasing. These two diseases are categorized as inflammatory bowel disease (IBD). Even there has been some theurapetic option for patient with these diseases, but surgery still the only option to treat fibrotic strictures. Tetrandrine was chosen as drug in this research because of its antifibrotic effect. This research was conducted to develop and evaluate calcium pectinate beads exploiting pH sensitive property for colon-targeted delivery of tetrandrine. Beads were prepared by ionotropic gelation method followed by enteric coating with HPMCP HP-55 or CAP. Uncoated beads were evaluated for particle size, shape, morphology, swellability, process efficiency and encapsulation efficiency. From evaluation, beads with concentration of calcium chloride 5% (formula 1) was chosen as formula for coating. First formula were more spherical in shape, not too sticky, and smaller in size when compared with beads using calcium chloride concentration 10% (formula 2) and 15% (formula 3). Encapsulation efficiency of the three formula, 65.67 ± 0.39%, 68.03 ± 0.12%, 56.28 ± 0.2% respectively. After coating process, beads were used in in vitro drug release and targeted test. The studies showed that coated calcium pectinate beads were sufficient to resist tetrandrine released in acidic medium, but was unsuccessfully in targeting colon."
Depok: Fakultas Farmasi Universitas Indonesia, 2016
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Dyah Suci Handayani
Pengaruh pemberian mangiferin terhadap ekspresi MRNA transforming growth factor beta pada hati tikus yang diinduksi tioasetamid = Effects of mangiferin on MRNA expression of transforming growth factor beta in rats with liver fibrosis induced by thioacetamide
"ABSTRAK
Latar Belakang: Mangiferin merupakan senyawa bioaktif yang diketahui dapat menghambat fibrosis, yaitu proses reversibel akibat jejas pada hati. Penelitian ini bertujuan untuk membuktikan efek mangiferin dalam menghambat fibrogenesis melalui hambatan terhadap ekspresi mRNA TGF-?, yaitu sitokin profibrogenik utama. Metode: Penelitian eksperimental ini menggunakan RNA yang diisolasi untuk mendapatkan cDNA, di mana kelompok perlakuan diberikan tioasetamid 200 mg/kg BB sebanyak 3 kali/minggu selama 5 minggu oleh Arozal W, dkk. Perlakuan dibagi menjadi kelompok kontrol, tioasetamid 200 mg/kg BB, tioasetamid 200 mg/kg BB mangiferin 50 mg/kg BB/hari, dan tioasetamid 200 mg/kg BB mangiferin 100 mg/kg BB/hari. Tingkat ekspresi mRNA TGF-? diukur dengan RT-PCR dan dihitung dengan metode Livak. Hasil: Terdapat peningkatan ekspresi mRNA TGF-? pada kelompok yang hanya diberikan tioasetamid terhadap kelompok kontrol. Pada kelompok yang diberikan mangiferin, terdapat penurunan ekspresi mRNA TGF-? terhadap kelompok yang hanya diberikan mangiferin, dengan penurunan ekspresi mRNA pada kelompok yang diberikan mangiferin 50 mg/kg BB/hari lebih besar. Kesimpulan: Mangiferin dapat menurunkan tingkat ekspresi mRNA TGF-? pada hati tikus yang diinduksi oleh tioasetamid, namun efeknya tidak linear antara dosis mangiferin dengan respon yang diberikan.
ABSTRACT
Background Mangiferin is a bioactive compound that is known to inhibit fibrosis, a reversible process that occurs as a result of liver injury. This study aims to prove the antifibrotic effect of mangiferin through inhibition of mRNA expression of TGF , which is the main profibrogenic cytokine. Method This experimental design uses isolated RNA to get cDNA, which treatment groups are given thioacetamid with dose of 200 mg kg BW, three times a week for five weeks consecutively, in study from Arozal W, et al. The treatment groups are control group, thioacetamid 200 mg kg BW, thioacetamid 200 mg kg BW mangiferin 50 mg kg BW day, and thioacetamid 200 mg kg BW mangiferin 100 mg kg BW day. The expression of TGF mRNA is measured with RT PCR and quantified with Livak method. Result There is an increase mRNA expression of TGF in group that was given thioacetamide only compare to the control group. In the groups that were on treatment of mangiferin, the mRNA expressions of TGF are lower than the thioacetamid only group. The mRNA expression of TGF of the group that was given mangiferin of dose 50 mg kg BW day is also lower compare to the group that was given higher dose of mangiferin. Conclusion Mangiferin can lower the expression of TGF in rats liver induced by thioacetamide. However, mangiferin does not give linear effect between dose and response."
2017
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