Hasil Pencarian  ::  Simpan CSV :: Kembali

"ABSTRAKThe growth of C. albicans is influenced by glucose intake. Xylitol is commonly used as sugar substitute. Reported effective concentrations of xylitol in reducing C. albicans growth in vitro were varied, 1%, 5%, and 10%. Objectives: Investigate the effect of different concentration and duration of xylitol exposure in inhibiting C. albicans growth in vitro. Method: Identification of C. albicans from oral swab of a male candidiasis patient was conducted using CHROMagar, confirmed by germ tube test. C. albicans suspension (108 cells/µl) were inoculated in SDB contained 1%, 5%, 10% xylitol, and without xylitol (as control), for 3 and 7 days, then incubated in 37oC on SDA and counted for their CFU after 48 hours. The C. albicans ATCC 10231 strain was used as acomparison. Results: After 3 days, increased concentration of xylitol (1%, 5%, 10%) lead to decrease growth of C. albicans, both the ATCC 10231 (125%; 51%; 14% respectively) and the clinical isolate (103%; 81%; 42%), p = 0.044. Significant lower growth of C. albicans compared to control were only seen in those exposed to 10% xylitol (p = 0.024). After 7 days, exposure of 1%, 5%, 10% xylitol did not significantly affect the growth of C. albicans (p = 0.396). Conclusion: The growth of C. albicans could be inhibited by 10% xylitol for 3 days."

"Latar belakang: Pembentukan biofilm dan sekresi enzim hidrolase merupakan faktor virulensi Candida albicans. Salah satu enzim hidrolase yang disekresikan adalah enzim proteinase yang banyak diekspresikan pada biofilm matur Candida albicans. Temulawak yang merupakan tanaman berkhasiat obat khas Indonesia yang diketahui mampu menghambat pertumbuhan biofilm dan aktivitas enzim fosfolipase pada Candida albicans planktonik.Tujuan: Melihat aktivitas enzim proteinase pada biofilm Candida albicans yang telah terinhibisi ekstrak etanol temulawak.Metode: Pemaparan ekstrak etanol temulawak pada biofilm Candida albicans yang telah diinkubasi 90 menit, lalu diinkubasi lebih lanjut hingga mencapai fase awal (6 jam), menengah (24 jam), dan maturasi (48 jam). Biofilm yang telah terinhibisi dipindahkan pada media uji berupa bovine serum albumin agar (BSAA). Aktivitas enzim proteinase dianalisis dengan mengukur zona proteolysis yang terbentuk di luar zona koloni Candida albicans pada BSAA.Hasil: Fase awal biofilm Candida albicans yang telah terinhibisi ekstrak etanol temulawak tidak terjadi aktivitas enzim proteinase, sementara pada fase menengah dan maturasi terlihat ada aktivitas enzim proteinase tetapi tidak setinggi aktivitas enzim proteinase pada kontrol negatif.Kesimpulan: Terjadi penurunan aktivitas enzim proteinase pada berbagai fase pertumbuhan biofilm Candida albicans Candida albicans yang telah terinhibisi oleh ekstrak etanol temulawak.

---

Background: Biofilm formation and hydrolase enzyme secretion are the virulence factor of Candida albicans. One of the hydrolase enzyme is secreted aspartyl proteinase or proteinase enzyme. This enzyme expressed more on mature biofilm of Candida albicans rather than on Candida albicans planktonic. Java turmeric is a native Indonesian plant which is known to have inhibition effect toward Candida albicans biofilm and could decrease the phospholipase enzyme activity of planktonic Candida albicans.Objective: To observe the activity of proteinase enzyme in Candida albicans biofilm that had been inhibited by Java turmeric ethanol extract.Method: exposure of Java turmeric ethanol extract to Candida albicans biofilm that had been incubated for 90 minutes, was followed by further incubation to reach early phase (6 hours), intermediate phase (24 hours), and maturation phase (48 hours) of biofilm formation. Inhibited biofilm then moved to the solid medium containing bovine serum albumin agar (BSAA). The activity of proteinase enzyme was analyzed by measuring the proteolytic zone seen outside the zone of Candida albicans colony on the BSAA.Result: No activity of proteinase enzyme showed on early phase of biofilm formation that had been inhibited by Java turmeric ethanol extract. On intermediate and maturation phase of biofilm that had been inhibited by Java turmeric ethanol extract showed high activity on proteinase enzyme although not as high as the activity of biofilm that had not been inhibited.Conclusion: The activity of proteinase enzyme is decreased on Candida albicans biofilm that had been inhibited by Java turmeric ethanol extract."

"Cases of oral candidiasis are commonly found, both in healthy individuals and immunecompromise patients, however publications of Candida carrier in the oral cavity of healthy population and risk factors for colonization in Indonesia are hardly available. Objective : This study was aimed to analyze the type and number of Candida colonies and identify risk factors in the oral cavity of apparenthly health FKG UI students. Material and methods : the specimens were taken from 195 subjects with oral rinse technique for identification using culture medium CHROMagar® and Sabaraoud dextrose agar. Results and discussion : Candida species were found in the 107 subjects oral cavity (54.87%), being Candida albicans was is the predominant species (52.33%). Some 88 subjects (82.24%) was dominant in the number of colonies <400 CFU/ml, while the rest had colony of >400 CFU/ml (17.76%). Candida colony grew dominantly in single colony (90.65%), and the others showed multi-species colonies (9.34%). Risk factors identified included age; gender; hormonal; blood type O; denture; orthodontic appliances; unstimulated salivary flow; pH of saliva; smoking, alcohol and oral cleaning habit; and oral health status. By using a statistical Pearson chi-square test, no significant relationship was found between risk factors and number of Candida colonies in the oral cavity p<0.05. Conclusion : there was no one single risk factor for Candida colonization, but combination of various risk factors for demographis, local and systemic was observed."

"Latar Belakang:.Pembedahan kepala dan leher merupakan tindakan yang kompleks dan penuh tantangan karena berhubungan dengan pencernaan dan pernapasan. Dengan angka kejadian komplikasi yang cukup tinggi yaitu 17%, dibutuhkan tolok ukur yang dapat memprediksi komplikasi pascabedah terutama di bidang bedah mulut dan maksilofasial. Sistem skoring APACHE II pada penelitian terdahulu terbukti efektif dalam memprediksi kejadian komplikasi pascabedah reseksi dan rekonstruksi mandibula. Tujuan Penelitian: Mengetahui efektivitas sistem skoring APACHE II sebagai prediktor komplikasi pascabedah reseksi dan rekosntruksi tumor jinak mandibula. Metode Penelitian: Studi retrospektif tahun 2015 – 2020 pada subjek yang memenuhi kriteria inklusi. Data diambil dari rekam medis pasien baik tertulis maupun digital. Analisis variabel kategorik dengan Uji Chi Square. Uji Mann-Whitney U untuk perbandingan rerata skor dua kelompok. Efektivitas skor APACHE II dinilai berdasarkan kurva ROC dan luas area dibawah kurva. Hasil: Dari 62 subjek penelitian, sebanyak 6 responden (9.7%) mengalami komplikasi pascabedah. Tidak terdapat perbedaan bermakna antara semua variabel independen yang duji dengan peningkatan skor APACHE II (nilai p > 0.05). Rerata skor pada kelompok komplikasi lebih tinggi (4.83) namun pada Uji Mann-Whitney U nilai p > 0.05. Analisis ROC pada studi ini memiliki sensitivitas 50% dan spesifisitas 78.6% dengan nilai cut off point 5.5 dan luas area dibawah kurva ROC sebesar 0.558. Kesimpulan: Sistem skoring APACHE II terbukti efektif dalam memprediksi kejadian komplikasi pascabdedah reseksi dan rekonstruksi tumor jinak mandibula.

---

Background: Head and neck surgery is a complex and challenging procedure because it affect the digestion and respiration organ system. With a fairly high incidence of complications, namely 17%, an indicator is needed to predict postoperative

complications, especially in the field of oral and maxillofacial surgery. The APACHE II scoring system in a previous study proved to be effective in predicting the incidence of postoperative complications after mandibular resection and reconstruction. Objective: To determine the effectiveness of the APACHE II scoring system as a predictor of postoperative complications of mandibular resection and reconstruction of benign tumors. Methods: Retrospective study on subjects who met the inclusion criteria in the period of 2015 – 2020. The data is collected from the patient's medical record, both written and digital. Categorical variable is being analyze with Chi Square Test. While Mann-Whitney U test analyzing the comparison of the mean scores of the two groups. The effectiveness of the APACHE II score was assessed based on the ROC curve and the area under the curve. Results: Of the 62 research subjects, 6 respondents (9.7%) experienced postoperative complications. There was no significant difference between all tested independent variables with an increase in the APACHE II score (p value > 0.05). The mean score in the complication group was higher (4.83) but in the Mann-Whitney U test the p value was > 0.05. The ROC analysis in this study has a sensitivity of 50% and a specificity of 78.6% with a cut off point value of 5.5 and an area under the ROC curve of 0.558. Conclusion: The APACHE II scoring system proved to be effective in predicting the incidence of postoperative complications after surgical resection and reconstruction of benign mandibular tumors."

"Terlepasnya mahkota tiruan saat mengunyah merupakan masalah yang sering dijumpai pada pemakai mahkota tiruan atau gigi tiruan jembatan. Terutama pada mahkota gigi yang kecil, pendek dan konus. Penyebab utamanya adalah faktor retensi dan resistensi yang kurang pada gigi penyangga. Salah satu metode untuk meningkatkan retensi dan resistensi pada praparasi mahkota adalah pembuatan groove. Tujuan penelitian ini adalah untuk mengetahui perbedaan resistensi mahkota tiruan penuh logam dengan berbagai bentuk preparasi groove. Penelitian dilakukan secara eksperimental laboratoris menggunakan 24 spesimen, yang terdiri dari 6 spesimen preparasi mahkota tanpa groove, 6 spesimen dengan groove bentuk box, 6 spesimen dengan groove bentuk V dan 6 spesimen dengan groove bentuk half round pada masing-masing spesimen tesebut dilakukan uji kompresi. Nilai rerata gaya melepaskan mahkota tiruan logam pada groove berbentuk box (27,97 kgF+SD 1,08), bentuk V (6,15 kgF+SD 0,22), half round (1,77 kgF+SD 0,22) dan tanpa groove (0,95 kgF+SD 0,13). Preparasi groove bentuk box adalah terbaik resistensinya, diikuti bentuk V, half round dan tanpa groove. Penelitian ini dapat dijadikan rekomendasi untuk pemilihan bentuk groove bila para klinisi menjumpai kondisi gigi molar yang pendek dan konus.

---

Dental crown or bridges can occasionally come loose or come off from the tooth while chewing. Especially on small, short and conus teeth.The main cause is lack of retention and resistance to the teeth. There are several methods to increase retention and resistance on crown, inlay and onlay preparation, which is parallelism, groove preparation, crown buildup and surface roughness. The aim of this study wasto know the resistance differences of a full metal crown with various forms of groove preparation. This was experimentall laboratories, study using compressive strength test in 24 specimens in which 6 specimens without grooves preparation, 6 specimens with box-shaped groove, 6 specimens with V-shaped groove and 6 specimens with half round grooves. The mean value of metal crown that come off during test on box-shaped Groove (27,97 kgF+SD1,08), V-shaped (6,15 kgF+SD 0,22), half round (1,77 kgF+SD 0,12) and without groove (0,95 kgF+SD 0,13). It is concluded that resistance is best in box-shaped, followed by V-shaped, half round and without groove. When clinicians find short and conus molar teeth, it is recommended the use of groove to increase the resistance of the crown."

"Fibroblast growth factors (FGFs) regulate the proliferation and differentiation of various cells via their respective receptors (FGFRs). During the early stages of tooth development in fetal mice, FGFs and FGFRs have been shown to be expressed in dental epithelia and mesenchymal cells at the initial stages of odontogenesis and to regulate cell proliferation and differentiation. However, little is known about the expression patterns of FGFs in the advanced stages of tooth development. In the present study, we focused on FGF18 expression in the rat mandibular first molar (M1) during the postnatal crown and root formation stages. FGF18 signals by RT-PCR using cDNAs from M1 were very weak at postnatal day 5 and were significantly up-regulated at days 7, 9 and 15. Transcripts were undetectable by in situ hybridization (ISH) but could be detected by in situ RT-PCR in the differentiated odontoblasts and cells of the sub-odontoblastic layer in both crown and root portions of M1 at day 15. The transcripts of FGFR2c and FGFR3, possible candidate receptors of FGF18, were detected by RT-PCR and ISH in differentiated odontoblasts throughout postnatal development. These results suggest the continual involvement of FGF18 signaling in the regulation of odontoblasts during root formation where it may contribute to dentin matrix formation and/or mineralization."

"Based on scarce evidence, fatty acids have been described as anticariogenic. The aim was to evaluate the effect of different types of fatty acids on enamel demin- eralization and on the cariogenic properties of Strepto- coccus mutans biofilms on a biofilm/caries model. Mature biofilms of S. mutans UA159 growing on bovine enamel slabs were exposed to 10 % sucrose for 5 min, 3 times per day followed by exposure to a panel of free fatty acids, including monounsaturated (oleic), polyunsaturated (lino- leic) and saturated (stearic) fatty acids, in concentrations of 0.1, 1 and 10 mM for five additional minutes. Enamel demineralization was determined before and after the experiments by microhardness. Slabs were retrieved to analyze biofilm biomass, viable bacterial counts and polysaccharide production. Biofilms exposed to sucrose, followed by oleic and linoleic acids, showed less demin- eralization than sucrose alone (p \ 0.05). Biomass, S. mutans colonies and insoluble extracellular polysaccharide production were reduced from the biofilms treated with oleic and linoleic fatty acids (p \ 0.05). No differences with the positive control were observed with the saturated stearic acid. Poly and monounsaturated fatty acids presented to S. mutans biofilms after a cariogenic challenge appear to reduce demineralization on enamel and to interfere with cariogenicity of S. mutans biofilms"

"Latar Belakang: magnesium menjadi bahan yang menarik untuk implan karena magnesium dapat menyelesaikan dalam tubuh kita. Tetapi bahan magnesium standar memiliki kecepatan tinggi dari korosi dalam tubuh kita dan menghasilkan beberapa gas dalam proses, yang tubuh kita tidak bisa mentolerir. ECAP (Equal Chanel sudut Menekan) prosedur untuk magnesium dapat menghasilkan magnesium dengan partikel yang jauh lebih kecil. Partikel kecil berarti lagi korosi, berarti produksi gas jumlah yang lebih kecil. Biokompatibilitas bahan masih menjadi pertanyaan. Dalam penelitian ini kami menyelidiki ECAP magnesium partikel tenggelam dengan fibroblast dan garis sel osteoblas dan menggunakan real time PCR untuk mendeteksi produksi interleukin 6 sebagai indikator peradangan tingkat dan interleukin 8 sebagai penanda angiogenesis kemotaksis. kami juga melakukan uji MTT untuk melihat korelasi antara sitokin produk dan proliferasi dari garis sel. Tujuan: untuk mengamati tingkat peradangan dan sifat angiogenesis chemotactic dari ECAP magnesium dalam garis sel osteoblas dan fibroblast. Metode: assay MTT dan RT-PCR untuk mengevaluasi interleukin 6 dan interleukin 8 produksi fibroblast dan osteoblast garis sel. Evaluasi di 1,3,7days setelah perawatan.

---

Background: magnesium become interesting material for implant since magnesium can resolve in our body. But standard magnesium material had high speed of corrosion in our body and produce some gas in the process, which is our body can not tolerate. ECAP ( Equal Chanel Angular Pressing ) procedure to magnesium can produce magnesium with far smaller particle. Small particle means longer corrosion, means smaller amount gas production.

Biocompatibility of the material is still a question. In this study we investigated ECAP magnesium particle immersed with fibroblast and osteoblast cell line and using real time PCR to detect the production of interleukin 6 as an inflammation rate indicator and interleukin 8 as the chemotactic angiogenesis marker. we also perform MTT assay to see the correlation between cytokines products and proliferations of the cell lines.

Purpose: to observe the inflammation rate and chemotactic angiogenesis properties of the magnesium ECAP in the osteoblast and fibroblast cell line.

Method: MTT assay and RT-PCR to evaluate interleukin 6 and interleukin 8 production in fibroblast and osteoblast cell line. Evaluate at 1,3,7days after the treatments."

"Konsumsi jus jeruk terus meningkat dengan kehadiran jus jeruk dalam kemasan. Belum banyak penelitian yang dilakukan mengenai efek jus ini dibanding jus jeruk segar terhadap gigi. Tujuan: Mengetahui perbedaan penurunan pH plak setelah konsumsi jus jeruk kemasan dan jus jeruk segar. Metode: Subjek diberikan 3 perlakuan: mengonsumsi jus jeruk kemasan, jus jeruk segar, jus lemon segar. Nilai pH plak kemudian diukur setelah 1-30 menit. Hasil: Terdapat perbedaan bermakna penurunan nilai pH plak 1-10 menit setelah konsumsi jus jeruk kemasan, jus jeruk segar, jus lemon segar Kesimpulan: Penurunan pH plak setelah konsumsi jus jeruk kemasan lebih besar dibandingkan dengan jus jeruk segar.

---

Orange juice consumption has been increased with the presence of commercially orange juice. There had been little study about the effect of this juice compared to fresh fruit juice on human teeth.

Objective: To know the difference of pH dental plaque reduction, after consumption of commercially orange juice and fresh orange juice.

Methods: Subjects were given three treatments: consuming commercially orange juice, fresh orange juice, and fresh lemon juice. pH dental plaque was measured after 1-30 minutes

Results: The difference of pH dental plaque reduction is statistically significant after 1-10 minutes consuming commercially orange juice, fresh orange juice, fresh lemon juice.

Conclusion: The reduction of pH dental plaque was greater after consumption of commercially orange juice than after fresh orange juice."

"Tujuan penelitian ini untuk mengetahui pengaruh suhu terhadap gaya regang power chain ortodontik dalam larutan saliva buatan. Digunakan 56 power chain merek Ormco, tipe tertutup, yang diregangkan dengan jarak 40 mm pada plat akrilik yang direndam dalam larutan saliva buatan dan akuades. Gaya regang (gf) diukur menggunakan correx meter force gauge pada sebelum dan sesudah 210 menit perendaman. Terjadi penurunan gaya regang yang berbeda bermakna (p<0,05) pada berbagai suhu. Pengaruh media perendaman larutan saliva buatan dan akuades menghasilkan penurunan gaya regang pada perlakuan suhu 23℃ yang berbeda bermakna (p<0,05), tetapi pada 4℃, 23℃ dan 55℃ tidak berbeda bermakna.

---

The objective of this study was to determine the effect of temperature toward tensile force of orthodontic power chain in artificial saliva. Specimens of 56 power chain (ormco, closed type) were stretched 40 mm on acrylic plate and immersed in artificial saliva and aquadest. Tensile force (grf) were measured using correx meter force gauge, recorded the initial and final force after 210 minutes of immersion. A decline in tensile force showed significant difference (p<0,05) at various temperatures. The effect of different immersion medium at 23°C resulted significant difference (p<0,05), but at 4°C, 37 ℃ and 55 ℃ did not differ significantly."