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"ABSTRAKStreptococcus mutans (S. mutans) diketahui merupakan bakteri patogen utamadalam proses karies. Koloni S. mutans pada anak dapat terbentuk melaluitransmisi S. mutans yang terutama bersumber dari ibu. S. mutans serotipe c, e, danf diklasifikasikan berdasarkan pada komposisi kimia polisakarida spesifik serotipedan sering ditemukan pada sampel plak. Sampel plak didapatkan dari 66 pasanganak usia 3-5 tahun dan ibunya. Metode Polymerase Chain Reaction (PCR) yangdipakai dengan menggunakan primer gtfB dalam penelitian ini telahmengkonfirmasi keberadaan S. mutans pada 46 sampel plak pasang anak danibunya. Terdapat hubungan yang bermakna antara karies anak dan karies ibunya(p<0,05). Skor karies anak akan meningkat seiring dengan peningkatan skorkaries ibu. Distribusi S. mutans serotipe c ditemukan dalam proporsi yang banyak,sedangkan S. mutans serotipe e ditemukan paling sedikit pada sampel plak anakusia 3 – 5 tahun dan ibunya.Terdapat hubungan tidak bermakna antara S. mutansserotipe c dan e dengan status karies anak dan ibunya (p>0,05). Terdapathubungan sangat lemah, tidak bermakna antara S. mutans serotipe c dan e anakdengan ibunya (0,000 < r < 0,199; p>0,05).

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ABSTRACTStreptococcus mutans (S. mutans) are considered to be an important bacterialpathogen of dental caries. The major reservoir from which children acquire theseorganisms is their mothers. S. mutans is classified into three serotypes, c, e and f,based on the chemical composition of its cell surface serotype-specificpolysacharide. S. mutans serotypes c,e and f were reported to be frequentlyisolated from human dental plaque. Plaque samples were collected from 66 3- to5-years-old and mothers with caries. Polymerase chain reaction (PCR) methodusing gtfB primer in this research has confirmed S. mutans from 46 dental plaquesamples child-mother pairs. There is significant relationship between childrencaries score and mother caries score (p<0.05). Child caries score increases asmother caries score rise. Distribution of serotype c S. mutans has more prevalentdetected than serotype e S. mutans. There is no significant relationship (p>0.05)between serotype c/e S. mutans and child-mother caries score. There is also nosignificant relationship (0,000 < r < 0,199 ;p>0,05) between serotype c/e S.mutans in children and their mothers."

"ABSTRACTLatar Belakang: Karies gigi merupakan penyakit gigi dan mulut yang terbanyak di Indonesia dan dapat dicegah dengan cara menjaga kebersihan mulut salah satunya menyikat gigi yang dapat menurunkan bakteri Streptococcus mutan. Bakteri ini akan membentuk plak dan menghasilkan asam yang dapat menyebabkan demineralisasi jaringan keras gigi. Tujuan: Mengetahui perbedaan kuantitas bakteri Streptococcus mutan pada plak gigi antara menyikat gigi sebelum dan sesudah makan terhadap subjek yang berumur 19-22 tahun. Metode: Desain pada penelitian ini dengan menggunakan metode crossover. Pengambilan data dilakukan terhadap 20 orang subjek, yang mana dibagi secara random alokasi menjadi dua kelompok yang masing-masing akan dilakukan perlakuan menyikat gigi sebelum dan setelah makan dengan waktu washout selama seminggu. Hasil: Analisis statistik mengunakan metode uji mann-whitney diperoleh p-value 0,598 yang menunjukkan bahwa tidak terdapat perbedaan kuantitas bakteri Streptococcus mutan pada plak gigi yang signifikan antara menyikat gigi sebelum dan sesudah makan. Akan tetapi kuantitas bakteri Streptococcus mutan pada plak gigi dengan menyikat gigi sebelum makan yaitu 193.333 CFU/ml lebih besar di bandingkan bakteri Streptococcus mutan pada plak gigi dengan menyikat gigi setelah makan sebanyak 180.000 CFU/ml. Kesimpulan: Kuantitas bakteri Streptococcus mutan pada plak gigi dengan perlakuan menyikat gigi setelah makan lebih sedikit dibandingkan dengan menyikat gigi sebelum makan. Akan tetapi dari analisis statistik menunjukkan bahwa tidak terdapat perbedaan kuantitas bakteri Streptococcus mutan pada plak yang signifikan antara menyikat gigi sebelum dan sesudah makan.

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ABSTRACTDental caries is the most dental and oral disease in Indonesia and can be prevented by maintaining oral hygiene, one way is by toothbrushing which can reduce the bacteria Streptococcus mutan. These bacteria will become dental plaque and produce acid which can causes demineralization of hard tissue. Objective: To determine the different in the numbers of bacteria Streptococcus mutan in dental plaques between toothbrushing before and after eating in 19-22 years. Method: The design of this study using the crossover. Data retrieval was carried out on 20 subjects, which were randomized allocation in two groups with washout time for a week. Results: Analysis statistic using the mann-whitney test obtained p-value 0.598 that there was no significant difference between brushing teeth before and after eating. However, the number of bacteria Streptococcus mutan on dental by toothbushing before eating is 193,333 CFU/ml bigger than the number of bacteria Streptococcus mutan on dental plaque by toothbushing after eating is 180,000 CFU/ml. Conclusion: The number of bacteria Streptococcus mutan on dental plaque by toothbrushing after eating was less than the group brushing before eating. However, the results from analysis statistic showed that there is no statistically significant difference between the numbers of bacteria Streptococcus mutan brushing teeth before and after eating."

"Latar Belakang : Resesi gingiva penyebab dentin hipersensitif (DH). Streptococcus mutans dan Streptococcus sobrinus pada plak menghasilkan asam. Produk asam menyebabkan demineralisasi akar gigi. Tujuan: Menganalisis jumlah serta distribusi S. mutans dan S. sobrinus dari plak dan saliva penderita resesi gingiva dengan DH dan non sensitif. Metode: Dari sampel saliva dan plak subjek DH dan non sensitif diperiksa jumlah S. mutans dan S. sobrinus menggunakan real-time PCR dengan SYBR Green. Hasil: Jumlah S. mutans lebih banyak pada plak DH daripada non sensitif, S. sobrinus lebih banyak pada saliva non sensitif. Kesimpulan: Jumlah S. mutans lebih banyak pada plak penderita DH.

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Background : Gingival recession cause of dentine hypersensitivity (DH). Streptococcus mutans and Streptococcus sobrinus in dental plaque will produce of acid. Acid can cause demineralization that involved in hypersensitivity.

Objectives : To analyze the amount and distribution of S. mutans and S. sobrinus from plaque and saliva in patients with DH and non sensitive.

Methods :, S. mutans and S. sobrinus from saliva and plaque samples was quantify by real-time PCR using SYBR Green.

Results : The number of S. mutans is higher in plaque of DH and S. sobrinus is higher in saliva of non sensitive.

Conclusion : Patients with DH had higher level of S. mutans in plaque."

"Silver Diamine Fluoride SDF sudah terbukti sebagai cairan yang dijadikan sebagai agen antikaries. Namun Silver Diamine Fluoride dapat meninggalkan noda hitam pada permukaan gigi dimana Silver Diamine Fluoride tersebut diaplikasikan serta menghasilkan rasa metal. Propolis Fluoride adalah salah satu temuan untuk menggantikan Silver Diamine Fluoride sebagai agen antikaries tanpa memiliki efek samping yang sama. Tujuan: Tujuan dari penelitian ini adalah untuk mengevaluasi aktivitas antimikrobial dari Propolis Fluoride dalam menghamabat pembentukan biofilm S.mutans dan dibandingkan dengan Silver Diamine Fluoride. Terdapat 3 variasi konsentrasi dari Propolis Fluoride yang diuji yaitu 3,3 Ekstrak Propolis 2,139 Fluoride , 6,3 Ekstrak Propolis 2,139 Fluoride , dan 10 Ekstrak Propolis 2,139 Fluoride. Metode: Suspensi S.mutans dikultur di dalam Brain Heart Infusion cair dan dan agar. Lalu suspensi diteteskan ke dalam 96-well plate dan dipaparkan dengan Propolis Fluoride lalu diinkubasi di dalam suasana anaerob selama 20 jam. Dilanjutkan dengan pemaparan kristal violet 0,5 pada 96-well plate dan pembacaan untuk mengukur besarnya Optical Density menggunakan microplate reader dengan gelombang 570 nm. Hasil: Potensi Propolis Fluoride 10 EP 2,139 Fluoride setara dengan potensi Silver Diamine Fluoride 38 . dalam menghambat pembentukan biofilm S.mutans. Hasil uji ANOVA dari Propolis Fluoride 10 EP 2,139 Fluoride p = 0,08. Kesimpulan: Potensi Propolis Fluoride dalam menghambat pembentukan biofilm S.mutans dapat disetarakan dengan potensi inhibisi Silver Diamine Fluoride 38.

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Background: Silver Diamine Fluoride is a liquid that has been proven to be an anti caries agent. But it leaves a black stain on the surface of the teeth where it was aplicated and it also have a metalic taste. Propolis Fluoride is one of the finding to replace Silver Diamine Fluoride as an anti caries agent without having the same side effect.

Objective: The aim of this study is to evaluate the antimicrobial activity of Propolis Fluoride in inhibiting S.mutans biofilm formation in comparison to Silver Diamine Fluoride. There are three different variant of Propolis Fluoride concentration which is 3,3 Propolis Extract 2,139 Fluoride , 6,3 Propolis Extract 2,139 Fluoride , and 10 Propolis Extract 2,139 Fluoride.

Methods: The suspension of S.mutans were cultured in Brain Heart Infusion broth and agar. Then the suspension was shed into 96 well plate and combined with Propolis Fluoride. Later, 96 well plate were incubated in anaerobic enviroment for 20 hour. Crystal violet 0,5 then shed into the 96 well plate. The amount of biofilm inhibition were evaluated using microplate reader in 570nm wavelength to find the amount of the Optical Density OD. Then the concentration were measure in OD then converted into percentage.

Result: Propolis Fluoride 10 EP 2,139 Fluoride have the same potention in inhibiting biofilm formation of S.mutans as Silver Diamine Fluoride 38. The ANOVA result for the Propolis Fluoride 10 EP 2,139 Fluoride is p 0.08.

Conclusion: The potention of Propolis Fluoride in inhibiting biofilm formation of S.mutans can be compared with Silver Diamine Fluoride."

"Latar belakang: ECC menjadi masalah serius di Indonesia dan Dunia. Terdapat 3komponen ECC, yaitu gigi, mikroba, serta lingkungan rongga mulut yang dalam hal iniyaitu protein saliva. Penyebab dari ECC sendiri yaitu bakteri Streptococcus mutans.Tidak hanya itu, Candida albicans sering dihubungkan dengan Streptococcus mutanspada plak ECC. Namun, adanya riset di mana Candida albicans cenderung mengurangisifat kariogenik Streptococcus mutans menarik untuk diteliti. Tujuan: menganalisisperan protein saliva ECC terhadap pertumbuhan biofilm Streptococcus mutans danStreptococcus mutans dan Candida albicans (atau dual-spesies) di rongga mulut.Metode: Setiap sampel dilakukan uji SDS-Page untuk melihat apakah terdapatperbedaan profil protein antar setiap sampel. Lalu, sampel dilakukan pengenceranmenjadi 3 konsentrasi, kemudian diinkubasi bersama dengan Streptococcus mutansserta dual-spesies di dalam 96-well plate selama 24 jam dan 48 jam secara anaerob.Lalu, masing-masing biofilm dilakukan uji Crystal Violet Staining (untuk mendapatkannilai Optical density) serta Total Plate Count. Hasil: Tidak terdapat perbedaan profilprotein antara saliva ECC dengan laju alir saliva <30 detik, 30-60 detik, 30-60 detikbebas ECC. Pada variabel konsentrasi protein, terdapat perbedaan dan kenaikan nilairerata pada nilai Optical density biofilm pada Streptococcus mutans dan dual-spesies.Tidak terdapat perbedaan secara statistik antara konsentrasi protein saliva denganviabilitas mikroba pada biofilm Streptococcus mutans dan dual-spesies meski nilairerata menunjukkan penurunan viabilitas mikroba. Pada biofilm Streptococcus mutansdan dual-spesies, tidak terdapat perbedaan bermakna pada hasil uji Optical density danviabilitas mikroba berdasarkan variabel waktu inkubasi biofilm. Meski nilai reratamenunjukkan adanya penurunan pada Optical density Streptococcus mutans, kenaikanpada viabilitas mikroba Streptococcus mutans, dan kenaikan pada Optical densitysekaligus viabilitas mikroba dual-spesies, namun tidak memengaruhi nilaikomparasinya. Kesimpulan: Protein saliva dapat memengaruhi pembentukan biofilmbaik Streptococcus mutans maupun kombinasi dual-spesies Streptococcus mutansdengan Candida albicans. Waktu inkubasi biofilm tidak dapat memengaruhipembentukan biofilm Streptococcus mutans maupun kombinasi dual-spesiesStreptococcus mutans dengan Candida albicans

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Background: ECC is a serious problem in Indonesia and the world. There are 3components of ECC, namely teeth, microbes, and the oral environment, in this casesalivary protein. The cause of ECC itself is Streptococcus mutans. Not only that,Candida albicans is often associated with Streptococcus mutans in ECC plaques.However, the research in which Candida albicans tends to reduce the cariogenicproperties of Streptococcus mutans is interesting. Purpose: to analyze the role of theECC salivary protein on the growth of Streptococcus mutans and combination ofStreptococcus mutans and Candida albicans (or dual-species) biofilms in the oral cavity.Methods: Each sample was subjected to an SDS-Page test to see if there weredifferences in protein profiles between each sample. Then, the sample was diluted into 3concentrations, then incubated together with Streptococcus mutans and dual-species in96-well plates for 24 hours and 48 hours anaerobically. Then, each biofilm wassubjected to a Crystal Violet Staining test (to obtain Optical density value) and TotalPlate Count. Results: There was no difference in protein profile between salivary ECCwith salivary flow rates <30 seconds, 30-60 seconds, ECC-free 30-60 seconds. In theprotein concentration variable, there were differences and an increase in trend lines inthe Optical density value of biofilms in Streptococcus mutans and dual-species. Therewas no statistical difference between salivary protein concentrations and microbialviability in Streptococcus mutans and dual-species biofilms, although the trend lineshowed a decrease in microbial viability. In Streptococcus mutans and dual-speciesbiofilms, there were no significant differences in the Optical density test results andmicrobial viability based on the biofilm incubation time variables. Although the trendline showed a decrease in Optical density Streptococcus mutans, an increase inmicrobial viability of Streptococcus mutans, and an increase in Optical density as wellas dual-species microbial viability, it did not affect the comparative value. Conclusion:Salivary protein can influence biofilm formation for both Streptococcus mutans and thedual-species combination of Streptococcus mutans and Candida albicans. Biofilmincubation time could not affect the biofilm formation of both Streptococcus mutansand the dual-species combination of Streptococcus mutans and Candida albicans"

"Streptococcus mutans serotype C is a major causative agent to caries and is found predominantly in dental plaque and saliva. Dentifrice containing xylitol has been shown to inhibit the growth of mutans streptococci. The aim of the study was to identify in vitro the influence of dentifrice containing xylitol on S. mutans serotype C. The solution of dentifrice containing xylitol was first diluted with sterile aquadest at 1:1, and then to concentrations of 100%, 10%, 1%, 0.1%, 0.01%, and 0.001%, also with positive and negative controls. These solutions were exposed to S. mutans Serotype C by diffusion and dilution method. The results of the study show that the inhibition zone formed at concentration of 10% and 100%. There was a significant positive correlation between the concentration of dentifrice and the growth of mutans streptococci (p<0.05), with MBC point at 10%. In conclusion, dentifrice containing xylitol has an antibacterial effect and can inhibit the growth of S. mutans serotype C. Increasing concentration of dentifrice containing xylitol increases the size of the inhibition zone."

"ABSTRAKLatar Belakang: Streptococcus mutans adalah bakteri penyebab karies dan memiliki faktor virulensi yang tinggi sehingga dapat memicu pembentukan biofilm pada gigi. Propolis merupakan bahan alami yang memiliki sifat antibakteri. Permen propolis hisap UI diduga dapat menghambat pembentukan biofilm bakteri Streptococcus mutans yang diisolasi dari plak gigi. Tujuan: Mengetahui efek permen propolis hisap UI terhadap pembentukan biofilm Streptococcus mutans yang diisolasi dari plak gigi. Metode: Streptococcus mutans pada sampel plak subjek diambil sebelum dan setelah perlakuan. Sampel dikultur dalam medium TYS20B kemudian dilakukan kultur pada medium cair TYS Broth. Setelah itu dilakukan uji biofilm dengan crystal violet assay. Hasil: Hasil uji Wilcoxon menyimpulkan tidak terdapat perbedaan pembentukan biofilm Streptococcus mutans yang bermakna sebelum dan setelah konsumsi permen propolis (p=0,697) Kesimpulan: Setelah konsumsi selama tujuh hari, permen propolis tidak mempengaruhi pembentukan biofilm Streptococcus mutans yang diisolasi dari plak gigi.

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ABSTRACTBackground: Streptococcus mutans is one of the etiology of caries with high virulence factors which can improve its ability to form biofilm on dental surface. Propolis is a natural product which has antibacterial properties. Propolis candy made by University of Indonesia was expected to decrease the ability of Streptococcus mutans to form biofilm. Aim: To know the effect of propolis candy on biofilm formation ability of Streptococcus mutans isolated from dental plaque. Method: Streptococcus mutans specimen from dental plaque of 120 subjects were collected pre and post treatment. Plaque sample then cultured on agar medium TYS20B and then cultured on TYS Broth medium. Then, biofilm formation of Streptococcus mutans from sample evaluated by crystal violet assay. Result: The results of Wilcoxon test conclude that there is no statistically significant difference between the biofilm formation of Streptococcus mutans before and after consumption of propolis candy (p=0,697) Conclusion: After seven days of consumption, propolis candy has no effect on biofilm formation of Streptococcus mutans isolated from dental plaque."