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Abstrak :
Masalah: Pemakaian gigi tiruan sebagian lepasan sangat erat hubungannya dengan terjadinya akumulasi plak dan depositnya, yang menjadi tempat menguntungkan untuk pertumbuhan bakteri. Pada gigi tiruan sebagian, penumpukan plak paling banyak terdapat di daerah servikal yang berhadapan dengan gigi penyangga, sehingga bakteri dapat pula berkoloni pada gigi penyangga dan menyebabkan karies gigi.Oleh karena itu sangat diperlukan pembersihan gigi tiruan yang dapat mengurangi pertumbuhan bakteri khususnya bakteri Streptococcus mutans yang berhubungan dengan etiologi karies. Telah terbukti bahwa pembersihan gigi tiruan secara kimiawi yaitu dengan cara perendaman dalam larutan pembersih seperti alkalin peroksida, sodium bikarbonat dan sodium hipoklorid 0,5% lebih efektif menjangkau seluruh permukaan basis gigi tiruan dibandinngkan pembersihan secara mekanik Tujuan: Untuk mengetahui lama perendaman larutan pembersih gigi tiruan yaitu alkallin peroksida, sodium bikarbonat dam sodium hipoklorid 0,5% yang dapat mengurangi jumlah koloni S.mutans pada basis resin akrilik permukaan halus dan kasar. Metode: Penelitian dilakukan secara eksperimental laboratoris menggunakan 48 spesimen, 24 spesimen dengan permukaan halus dan 24 spesimen dengan permukaan kasar. Setelah dikontaminasi dengan bakteri S.mutans direndam dalam 3 larutan pembersih dan aquades sebagai kontrol selama 5 dan 10 menit. Selanjutnya spesimen dibiakkan pada agar darah, dimasukkan inkubator dan jumlah koloni dihitung dan dianalisa. Hasil: Dari hasil uji statistik disimpulkan bahwa larutan sodium hipoklorid 0,5% dengan lama perendaman selama 5 menit tidak berbeda bermakna dengan perendaman selama 10 menit pada spesimen resin akrilik heat-cured permukaan halus dan permukaan kasar. Sodium hipoklorid 0,5% paling efektif mengurangi bakteri S.mutans dibandingkan dengan larutan alkalin peroksida dan sodium bikarbonat Kesimpulan: Larutan sodium hipoklorid 0.5% dengan lama perendaman 5 dan 10 menit paling banyak mengurangi jumlah koloni S mutans. ...... Background:The usage of partial removable denture is strongly associated with accumulation of plaque and its deposits, which is an ideal place for bacterial growth. Plaque deposits in partial removable denture commonly found in cervical area adjacent to abutment tooth and caused bacterial colonization on abutment tooth which led to the occurrence of dental caries. That is why application of denture cleaning solution that will reduce bacterial growth, especially Streptococcus mutans which related to caries formation etiology, is crucial. It has been proven that chemical cleansing of denture by soaking the removable denture in chemical cleaning solution such as sodium hypochlorite 0,5% and sodium bicarbonate is more effective the area inaccessible by mechanical cleansing. Objective:To determine the effect of rinsing duration of cleaning solution, such as sodium bicarbonate and sodium hypochlorite 0,5%, to S.mutans bacterial colonies on smooth-surfaced and rough-surfaced acrylic resin plate. Method:This laboratory experiment was conducted using 48 specimens, with 24 smooth-surfaced and 24 rough-surfaced acrylic resin plates. After S.mutans contamination, the specimens were rinsed in 3 different cleaning solution and aquadest which served as control, for the duration of 5 and 10 minutes. Afterwards, the specimens were cultured in blood agar mediums and kept inside incubator for a period of time, and then colonies of S. mutans formed in the medium were counted. Results:Statistical analysis showed that the rinsing of acrylic plate in sodium hypochlorite 0,5% for 5 and 10 minutes significantly reduced S.mutans colonies compared to rinsing in alkaline peroxide and sodium bicarbonate for both the smooth and rough-surfaced specimens. Conclusion:Soaking of acrylic plate in sodium hypochlorite 0,5% for 5 and 10 minutes is the most effective way to reduce S.mutans colonies in both the smooth and rough-surfaced specimens.

Abstrak :
S.mutans dikatakan sebagai salah satu penyebab utama karies. Bakteri ini dinyatakan sebagai bakteri pertama yang dapat melekat dan berkoloni pada permukaan gigi dan menyebabkan plak terbentuk secara terus menerus, dan terjadinya penurunan pH plak. Probiotik adalah suatu mikroorganisme hidup yang apabila dipergunakan dalam jumlah yang cukup, memberikan manfaat kesehatan bagi host. Berdasarkan berbagai penelitian, berbagai produk probiotik dapat mempengaruhi bakteri-bakteri penyebab karies gigi, terutama S.mutans. Penelitian ini dilakukan untuk mengetahui perbedaan jumlah koloni S.mutans dalam plak anak sebelum dan sesudah kumur minuman probiotik. Pengambilan sampel plak dilakukan terhadap 13 subyek dan dilakukan pertama kali yaitu sebelum memulai kumur minuman probiotik. Setelah itu subyek diinstruksikan untuk kumur minuman probiotik selama 7 hari dan pada saat hari ke 3 dan ke 7 kumur minuman probiotik sampel plak diambil kembali. Hasil penelitian memperlihatkan penurunan jumlah koloni S.mutans dari sebelum kumur minuman probiotik, kemudian pada hari ke 3 kumur, hingga setelah kumur minuman probiotik selama 7 hari. Hasil perhitungan statistik menunjukkan bahwa kumur minuman probiotik selama 3 dan 7 hari dapat menurunkan jumlah koloni S.mutans dalam plak gigi anak secara bermakna dibanding dengan sebelum kumur (p = 0,001). ......S.mutans is said as one of the major etiology of caries. This bactery is said to be the first bactery that sticked and colonized on the tooth surface and caused the continuity of plaque formation, also the decrease of plaque?s pH. Probiotic is living microorganisms that, if used in adequate amount, will give health benefits to the host. Based on previous researches, various products of probiotic can influence caries etiology bacterias, especially S.mutans. The aim of this study is to know the differences of S.mutans colonization total amount before and after rinsing with probiotic drink. The plaque samples were first taken from 13 subjects before starting the probiotic oral rinse. After that subjects were instructed to rinse with probiotic drink for 7 days, and then in the 3rd and 7th days of rinsing, the plaque samples were taken again. The study showed that after 7 days rinsing with probiotic drink, the total amount of S.mutans colonization was found decreasing on the 3rd day and continued to the 7th day. Statistic count showed that rinsing with probiotic drinks for 3 and 7 days can make a significant difference on the amount of S.mutans colonization than before rinsing (p = 0,001).

Abstrak :
Probiotik adalah mikroorganisme hidup yang ketika diberikan dalam jumlah yang tepat dapat memberikan manfaat bagi kesehatan host. Lactobacillus Casei merupakan salah satu contoh bakteri asam laktat yang digunakan dalam probiotik. Bakteri ini dapat mencegah adhesi dan invasi bakteri patogen, memodifikasi lingkungan usus dan memodulasi respon imun. Penelitian ini dilakukan untuk mengetahui perbedaan jumlah koloni S.mutans pada plak gigi anak sebelum dan setelah minum minuman probiotik di Jakarta. Subyek penelitian berusia 9-12 tahun, sebanyak 13 orang anak. Sampel penelitian berupa koloni S.mutans yang terdapat dalam plak gigi anak. Jumlah koloni diukur dengan colony forming unit. Hasil penelitian memperlihatkan adanya perbedaan rerata jumlah koloni S.mutans pada hari ketiga dan ketujuh, sebelum dan setelah minum probiotik. Pada perhitungan statistik ditemukan perbedaan bermakna antara jumlah koloni S.mutans pada plak gigi anak sebelum dan setelah minum minuman probiotik. ......Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Lactobacillus Casei is one example of lactic acid bacteria used in probiotics. These bacteria may prevent bacterial adhesion and invasion of pathogens, modify the intestinal environment and modulate the immune response. This research was conducted to determine the differences of total S.mutans colony on children dental plaque before and after probiotics consumption in Jakarta. Subjects aged 9-12 years, 13 children. Research sample are S.mutans on children dental plaque. Total S.mutans colony were measured using colony forming unit. The results showed a mean difference between total S.mutans colony on children dental plaque, on the third day and the seventh day, before and after probiotics consumption. From the results of statistical analysis showed significant differences between total S.mutans colony on children dental plaque before and after probiotics consumption.

Abstrak :
Salah satu penyakit infeksi mulut dengan prevalensi tinggi di Indonesia adalah karies gigi. Hasil SKRT tahun 2009 mencatat bahwa 73% penduduk Indonesia menderita karies gigi. Streptococcus mutans merupakan agen utama penyebab karies gigi. Telah diketahui bahwa umbi binahong mempunyai sifat antibakteri. Oleh karena itu, penelitian ini bertujuan untuk membuktikan efek antibakteri infusum umbi binahong terhadap Streptococcus mutans secara in vitro. Infusum dibuat dalam 4 konsentrasi berbeda: 100%, 50%, 25% dan 10%. Uji dilusi dilakukan dengan media BHI cairdan TYS20B untuk mengetahui KHM dan KBM. Uji difusi menggunakan media BHA dan BHA darah untuk mengetahui besar zona hambatan. Dari uji dilusi, didapatkan KHM 25% dan KBM diatas 25%. Dari uji difusi pada media BHA, didapatkan besar zona hambatan: 1,375 mm (10%), 1,125 mm (25%), 0,75 mm (50%) dan 1,25 mm (100%). Dari uji difusi pada media BHA darah, didapatkan besar zona hambatan: 1,5 mm (10%), 1,625 mm (25%), 1,5 mm (50%) dan 0,75 mm (100%). Bakteri S. mutans sensitif terhadap infusum umbi binahong. ...... As one of the most common infectious oral diseases in Indonesia, dental caries has relatively high prevalence. Streptococcus mutans appears to be a prominent causative agent of caries. Evidence shows that binahong rhizomes have antibacterial properties. Thus, the aim of the research is to prove whether infusion of binahong rhizomes is effective as an antibacterial agent against Streptococcus mutans, in vitro. The infusion was made into four different concentrations: 100%, 50%, 25, and 10%. To determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), dilution test was performed on BHI broth and TYS20B mediums. As to measure the zone of inhibition, diffusion test was performed on Brain-Heart Agar and Brain-Heart-Blood Agar mediums. MIC is achieved on 25% concentration and the MBC is above 25%. Inhibitory zone results on BHA diffusion test are: 1.375 mm (10%), 1.125 mm (25%), 0.75 mm (50%) and 1.25 mm (100%). While on BHB Agar medium: 1.5 mm (10%), 1.625 mm (25%), 1.5 mm (50%) and 0.75 mm (100%). It is concluded that S. mutans is sensitive to binahong rhizomes infusion.

Abstrak :
ABSTRAK
Latar belakang: Early Childhood Caries disebabkan oleh adanya aktivitas dari bakteri kariogenik, terutama Streptococcus mutans, yang dapat menurunkan pH lingkungan mulut. Veillonella spp., bakteri koagregrat Streptococcus mutans, dapat menaikkan pH lingkungan mulut. Tujuan: Mengetahui perbandingan kuantitas Streptococcus mutans dan Veillonella spp. saliva pada anak kategori risiko karies rendah dan tinggi. Metode: DNA Streptococcus mutans. dan Veillonella spp. dari ekstraksi saliva subjek dikuantifikasi menggunakan qPCR. Hasil: Terdapat perbedaan bermakna antara jumlah bakteri pada anak dengan kategori karies rendah dan tinggi. Kesimpulan: Jumlah Streptococcus mutans maupun Veillonella spp. pada saliva anak dengan kategori risiko karies tinggi lebih banyak daripada risiko karies rendah.

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ABSTRACT
Background Early Childhood Caries is caused by cariogenic bacteria rsquo s activity mainly Streptococcus mutans which decrease the oral environment rsquo s pH Otherwise Veillonella spp coaggregration of Streptococcus mutans can raise the oral environment rsquo s pH Aim To examine the quantity comparison of Streptococcus mutans and Veillonella spp in children rsquo s saliva with high risk and low risk caries Methods Quantification ofDNA Streptococcus mutans and Veillonella spp extracted from subject rsquo s saliva using qPCR Results There were significant differences between the number of bacteria in children with high risk and low risk caries Conclusion There is a higherquantity of Streptococcus mutans and Veillonella spp in children rsquo s saliva with high risk caries than low risk caries

Abstrak :
ABSTRAK
Latar Belakang: Early Childhood Caries (ECC) disebabkan oleh aktivitas Streptococcus mutans dengan cara memetabolisme karbohidrat menjadi asam laktat. Salah satu bakteri yang memfermentasikan asam laktat adalah Veillonella spp. Tujuan: Mengetahui perbandingan kuantitas Streptococcus mutans dan Veillonella spp. plak lidah anak kategori risiko karies rendah dan tinggi. Metode: Kuantitas Streptococcus mutans dan Veillonella spp. dari sampel plak lidah dikuantifikasi menggunakan qPCR. Hasil: Kuantitas Streptococcus mutans dan Veillonella spp. lebih banyak pada kategori risiko karies tinggi dibandingkan risiko karies rendah. Kesimpulan: Kuantitas Streptococcus mutans dan Veillonella spp. pada plak lidah anak kategori risiko karies rendah dan tinggi tidak berbeda bermakna secara statistik.

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ABSTRACT
Background: Early Childhood Caries (ECC) is caused by the activity of Streptococcus mutans by metabolize carbohydrates into lactic acid. One of the bacteria that fermenting lactic acid is Veillonella spp. Objectives: To determine the comparison of Streptococcus mutans and Veillonella spp. quantity in tongue plaque of children with low-risk and high-risk caries. Methods: Quantity of Streptococcus mutans and Veillonella spp. from tongue plaque samples were quantified using qPCR. Results: Quantity of Streptococcus mutans and Veillonella spp. in high-risk caries is higher than low-risk caries. Conclusion: There were no significant differences between Streptococcus mutans and Veillonella spp. quantity in tongue plaque with children with low-risk and high-risk caries.

Abstrak :
Latar belakang : S. mutans merupakan patogen utama penyebab karies. NSF diketahui memiliki sifat antibakterial. Tujuan : Menganalisis pengaruh NSF dalam menghambat virulensi dan pembentukkan biofilm S. mutans. Metode : Suspensi bakteri S. mutans dalam media BHI yang diperkaya sukrosa 0.2 dipaparkan NSF diinkubasi selama 20 jam. Persen inhibisi biofilm dinilai menggunakan uji crystal violet. Hasil : Nilai KHM NSF adalah 2.66 dan nilai KBM 4.16 . NSF mampu menghambat pembentukkan biofilm S. mutans. Kesimpulan : NSF mampu menghambat virulensi dan pembentukkan biofilm S. mutans. ......Background: S. mutans are the primary pathogens that cause caries. NSF known to have antimicrobial properties. Aim: To analyze the effect of NSF in inhibiting virulence and biofilm formation of S. mutans. Methods: Bacterial suspension of S. mutans in BHI medium enriched 0.2 sucrose exposed with NSF incubated for 20 hours. Percent inhibition of biofilm was assessed using crystal violet test. Result: NSF MIC value is 2.66 and MBC value is 4.16 . NSF is able to inhibit biofilm formation of S. mutans. Conclusion: NSF is able to inhibit virulence and biofilm formation of S.mutans.

Abstrak :
Latar Belakang: Propolis fluoride salah satu sediaan yang dapat menghambat perkembangan bakteri penyebab karies. Tujuan: Menganalisis pengaruh propolis fluoride terhadap viabilitas biofilm S. mutans dalam berbagai fase. Metode: Model biofilm S.mutans di inkubasi selama 4 jam fase adesi, 12 jam fase akumulasi aktif, dan 24 jam fase maturasi, kemudian dipaparkan dengan propolis fluoride 3,3 ; 6,6, 10 kelompok perlakuan, dan SDF 38 kelompok kontrol. Analisis Viabilitas biofilm S.mutans dilakukan dengan uji MTT untuk dibaca pada microplate reader. Hasil: Pada pemaparan Propolis 3,3, persentase viabilitas biofilm S.mutans pada fase adesi 14,89 3,19; fase akumulasi aktif 24,37 7,43; dan fase maturasi 21,35 3,06. Pada pemaparan Propolis 6,6, persentase viabilitas biofilm S.mutans pada fase adesi 10,10 2,43; fase akumulasi aktif 20,88 13,17; dan fase maturasi 18,82 4,51. Pada pemaparan Propolis 10, persentase viabilitas biofilm S.mutans pada fase adesi8,04 1,59; fase akumulasi aktif 11,17 8,90; dan fase maturasi 16,75 1,83. Kesimpulan: Propolis fluoride 10 dapat menurunkan viabilitas biofilm S.mutans pada fase adesi. ......Background: Propolis fluoride in one of dosage could inhibit the growth of bacteria that cause caries. Objective: To analyze the effect of propolis fluoride on the viability of S. mutans biofilm in various phases. Method: S. mutans biofilm models were incubated for 4 hours adhesion phase, 12 hours active accumulation phase, and 24 hours maturation phase, then presented with propolis fluoride 3.3 6.6, 10 treatment group, and SDF 38 control group. Analysis of S. mutans biofilm viability is tested by MTT in the microplate reader. Results: Exposure of Propolis Flouride 3.3, the percentage of S. mutans biofilm viability in the adhesion phase is 14.89 3.19 active accumulation phase is 24.37 7.43 and the maturation phase is 21.35 3.06. On exposure of Propolis Flouride 6.6, the percentage of S. mutans biofilm viability in adhesion phase is 10,10 2,43 active accumulation phase is 20.88 13.17 and the maturation phase is 18.82 4.51. On exposure of Propolis Fluoride 10, the percentage of S. mutans biofilm viability in the phase of adhesion is 8.04 1.59 active accumulation phase is 11.17 8.90 and the phase of maturation is 16.75 1.83. Conclusion: Propolis fluoride 10 could reduced the viability of S. mutans biofilm in adhesion phase.

Abstrak :
Latar Belakang : Ekstrak kismis telah dikenal sejak dahulu dalam menghambat pertumbuhan bakteri patogen, karena mengandung oleanolic acid yang telah terbukti dapat menghambat pertumbuhan bakteri rongga mulut. Tujuan: Penelitian ini bertujuan untuk membuktikan efek antimikroba infusum Kismis terhadap Streptococcus mutans. Metode: Infusum Kismis dibuat dengan proses pemanasan 100oCselama 15 menit pada 50 gr kismis dalam 500ml air (konsentyrasi 10%), kemudian diopanaskan lagi sehingga larutan tersisa 50ml (konsentrasi 100%). Untuk penelitian ini dibuat infusum 80%, 60%, 40%, 30%, dan 15% sesuai dengan prosedur yang benar. Efek antimikroba masing2 infusum kismis diperiksa dengan metode dilusi sehingga diperoleh nilai KHM dan KBM serta metode difusi sehingga diperoleh nilai Zona Hambatan terhadap 6 koloni streptococcus mutans. Hasil: Efek infusum Kismis terhadap Streptococcus mutans adalah sebagai berikut : Pada koloni 1 : zona hambatan 1,00 mm; KHM 30% /ml ,KBM 60% /ml ; koloni 2 : zona hambatan 1,50 mm; KHM 30% /ml ,KBM 60% /ml; koloni 3 : zona hambatan 1,00 mm; KHM 30% /ml ,KBM 60% /ml; koloni 4 : zona hambatan 0,50 mm; KHM 30% /ml ,KBM 60% /ml; koloni 5 : zona hambatan 1,00 mm; KHM 30% /ml ,KBM 60% /ml; koloni 6 : zona hambatan 1,00 mm; KHM 30% /ml ,KBM 60% /ml; Kesimpulan: Secara in vitro, infusum kismis dengan konsentrasi 30% bersifat bakteriostatik, sedangkan pada konsentrasi 60% bersifat bakterisid dengan rata-rata Zona hambatan 1,0625 mm.

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Background : Seedless Raisins has been known that it can inhibit the growth of pathogen bactery, because it contains of oleanolic acid that can inhibit the growth of oral pathogen. Objectives: The aim of the study is to determine the sensitivity of Infusum Raisins on mutans streptococci. Methods: Infusum is the product of the process of steeping Raisins for extraction of its medicinal principle. The effect of infusum Raisins was examined in vitro on the inhibit the bacterial growth by determining the inhibition zone (agar diffusion method), minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). The microorganisms tested were composed 6 colony of Streptococcus mutans wild strain that taken from Oral Biology Laboratory of Faculty of Dentistry University of Indonesia, labeled as Streptococcus mutans1, Streptococcus mutans2, Streptococcus mutans3, Streptococcus mutans4,Streptococcus mutans5, Streptococcus mutans6. Data obtained was done in a descriptive method. Results: showed that Raisins?s Infusum had effect on all of mutans of Streptococcus mutans 1 (inhibition zone 1.00 mm; MIC 30% /ml ,MBC 60% /ml); Streptococcus mutans 2 (inhibition zone 1.50 mm; MIC 30%/ml ,MBC 60%/ml); Streptococcus mutans 3 (inhibition zone 1.00 mm; MIC 30%/ml ,MBC 60%/ml); Streptococcus mutans 4 (inhibition zone 0.50 mm; MIC 30%/ml ,MBC 60%/ml); Streptococcus mutans 5 (inhibition zone 1.00 mm; MIC 30%/ml ,MBC 60%/ml), Streptococcus mutans6 (inhibition zone 1.00 mm; MIC 30/ml ,MBC 60%/ml). Conclusion: We concluded that Raisins's Infusum has anti microbial activity against 6 colony of Streptococcus mutans in oral cavity, in vitro. Hence it may have potential anti-cariesproperty.

Abstrak :
Latar Belakang : Jambu air Semarang (Syzygium samarangenase) atau jambu cincalo telah terbukti dapat menghambat pertumbuhan bakteri patogen, karena mengandung senyawa Tannin dan Oleanolic acid. Tujuan: Penelitian ini untuk membuktikan daya antimikroba infusum Jambu air Semarang terhadap Streptococcus mutans. Metode: Infusum Jambu air Semarang dibuat dengan proses pemanasan 100o C selama 15 menit terhadap 50 gram jambu air semarang dalam 500 ml air, kemudian disaring untuk mendapatkan 500 ml larutan (konsentrasi 10%), dipanaskan lagi sehingga larutan tersisa 50 ml (konsentrasi 100%), untuk penelitian ini dibuat infusum 80%, 60%, 40%, 30%, 20%, dan 15% sesuai prosedur yang benar. Efek antimikroba masing-masing konsentrasi infusum diperiksa dengan metode difusi serial dilusi sehingga diperoleh nilai KHM dan KBM serta metode difusi sehingga diperoleh nilai zona hambatan terhadap 6 koloni S.mutans. Hasil: Terhadap ke-6 koloni S.mutans diperoleh hasil sebagai berikut: KHM : 80%/ml dan KBM tidak diketahui serta rata-rata zona hambatan 1,533 mm. Kesimpulan: Secara in vitro, Infusum Jambu air Semarang dengan konsentrasi 80% berkhasiat menghambat pertumbuhan bakteri S.mutans(efek bakteriostatik).

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Background : Wax apple (Syzygium samarangenase) has been known to prevent the growth of pathogen bacteria since anciety because it is contain fenol (tannin) and oleanolic acid which had been proved to prevent the growth of bacteria. Objectives: This research are for determine the antimicroba activity of Wax apple?s infusum on Streptococcus mutans. Methods: Wax apple?s infusum was made by the process of steeping seedless 50 gram Wax apple in 500 ml water, to see it?s medicinal properties after getting 100% concentration of solution. After that we made 80%, 60%, 40%, 30%, 20%, and 15% infusum. The antimicrobial activity of wax apple?s infusum was examined by dilution method to get the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC), and diffusion method to get the inhibition zone to 6 colony of S.mutans. Data obtained from this research in a descriptive method. Results: Effect of Wax apple?s infusum on Streptococcus mutans are : Streptococcus mutans type 1 inhibition zone 1,5 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutans type 2 inhibition zone 1,5 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutans type 3 inhibition zone 1,4 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutans type 4 inhibition zone 1,6 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutans type 5 inhibition zone 1,7 mm; MIC 80% /ml ,MBC unknown; Streptococcus mutan type 6 inhibition zone 1,5 mm; MIC 80% /ml ,MBC unknown; Conclusion: We conclude that Wax apple?s Infusum has anti microbial activity against Mutans Streptococci, in vitro. Hence it may have potential anticariesproperty.