Hasil Pencarian  ::  Simpan CSV :: Kembali

"Latar belakang: Early Childhood Caries disebabkan oleh adanya aktivitas dari bakteri kariogenik, terutama Streptococcus mutans, yang dapat menurunkan pH lingkungan mulut. Veillonella spp., bakteri koagregrat Streptococcus mutans, dapat menaikkan pH lingkungan mulut. Tujuan: Mengetahui perbandingan kuantitas Streptococcus mutans dan Veillonella spp. saliva pada anak kategori risiko karies rendah dan tinggi. Metode: DNA Streptococcus mutans. dan Veillonella spp. dari ekstraksi saliva subjek dikuantifikasi menggunakan qPCR. Hasil: Terdapat perbedaan bermakna antara jumlah bakteri pada anak dengan kategori karies rendah dan tinggi. Kesimpulan: Jumlah Streptococcus mutans maupun Veillonella spp. pada saliva anak dengan kategori risiko karies tinggi lebih banyak daripada risiko karies rendah.

---

Background: Early Childhood Caries is caused by cariogenic bacteria's activity mainly Streptococcus mutans which decrease the oral environment's pH. Otherwise Veillonella spp coaggregration of Streptococcus mutans can raise the oral environment's pH.

Aim: To examine the quantity comparison of Streptococcus mutans and Veillonella spp in children's saliva with high risk and low risk caries.

Methods Quantification of DNA Streptococcus mutans and Veillonella spp extracted from subject's saliva using qPCR.

Results: There were significant differences between the number of bacteria in children with high risk and low risk caries.

Conclusion: There is a higherquantity of Streptococcus mutans and Veillonella spp in children's saliva with high risk caries than low risk caries."

"Latar Belakang: Early Childhood Caries (ECC) disebabkan oleh aktivitas Streptococcus mutans dengan cara memetabolisme karbohidrat menjadi asam laktat. Salah satu bakteri yang memfermentasikan asam laktat adalah Veillonella spp. Tujuan: Mengetahui perbandingan kuantitas Streptococcus mutans dan Veillonella spp. plak lidah anak kategori risiko karies rendah dan tinggi. Metode: Kuantitas Streptococcus mutans dan Veillonella spp. dari sampel plak lidah dikuantifikasi menggunakan qPCR. Hasil: Kuantitas Streptococcus mutans dan Veillonella spp. lebih banyak pada kategori risiko karies tinggi dibandingkan risiko karies rendah. Kesimpulan: Kuantitas Streptococcus mutans dan Veillonella spp. pada plak lidah anak kategori risiko karies rendah dan tinggi tidak berbeda bermakna secara statistik.

---

Background: Early Childhood Caries (ECC) is caused by the activity of Streptococcus mutans by metabolize carbohydrates into lactic acid. One of the bacteria that fermenting lactic acid is Veillonella spp.

Objectives: To determine the comparison of Streptococcus mutans and Veillonella spp. quantity in tongue plaque of children with low-risk and high-risk caries.

Methods: Quantity of Streptococcus mutans and Veillonella spp. from tongue plaque samples were quantified using qPCR.

Results: Quantity of Streptococcus mutans and Veillonella spp. in high-risk caries is higher than low-risk caries.

Conclusion: There were no significant differences between Streptococcus mutans and Veillonella spp. quantity in tongue plaque with children with low-risk and high-risk caries."

"Latar Belakang : Aktivitas fisik pada saat melakukan olahraga lari menstimulasi saraf simpatik yang dapat mempengaruhi sekresi dan komposisi saliva, termasuk salah satunya Streptococcus mutans-Binding Salivary Protein Protein saliva berperan dalam menghambat atau memfasilitasi pembentukan biofilm. Tujuan : Menganalisis pengaruh Streptococcus mutans-Binding Salivary Protein terhadap pembentukan biofilm P. gingivalis. Metode : Pemilihan subjek pelari dan nonpelari ditetapkan berdasarkan pengukuran VO2max dan riwayat lari rutin. Profil protein Streptococcus mutans-Binding Salivary Protein ATCC 25175 pada pelari dan nonpelari diidentifikasi dengan uji SDS PAGE. Uji pembentukan biofilm P. gingivalis ATCC 33277 dilakukan dengan pewarnaan crystal violet. Data yang didapat kemudian diolah menggunakan uji korelasi. Hasil : Streptococcus mutans-Binding Salivary Protein memfasilitasi pembentukan biofilm P. gingivalis ATCC 33277, baik pada waktu inkubasi 3 jam maupun 24 jam. Kesimpulan : Streptococcus mutans-Binding Salivary Protein yang diisolasi dari subjek pelari dan nonpelari memfasilitasi pembentukan biofilm P.gingivalis terhadap pembentukan biofilm P. gingivalis ATCC 33277.

---

Background : Physical activity during exercise induced sympathetic stimuli which may affect the secretion and composition of saliva, including anti S.mutans salivary protein. Salivary protein may act as inhibitor or facilitator on biofilm formation.

Objective : To analyze the effect of Streptococcus mutans Binding Salivary Protein towards the formation of Porphyromonas gingivalis biofilm.

Method : The runners and non runners subjects were selected using VO2max measurement and running routine history. Streptococcus mutans Binding Salivary Protein profile from the runners and non runners group were identified using SDS PAGE method. Biofilm assay with crystal violet was used to test the formation of P. gingivalis ATCC 33277. Data was done using correlation test.

Result : Streptococcus mutans Binding Salivary Protein facilitate P. gingivalis biofilm formation on 3 and 24 hours incubation time.

Conclusion : Streptococcus mutans Binding Salivary Protein from runners and non runners facilitate P. gingivalis ATCC 33277 biofilm formation."

"The presence of fixed orthodontic appliances leads to an increase in the absolute number of salivary Streptococcus mutans, as well as an increase in the percentage of S. mutans. These changes may be responsible for decalcification or white spot formation during orthodontic therapy. Decalcification or white spot formation during orthodontic treatment has been a problem since the introduction of fixed appliances. The white spot lesion is considered to be the precurser of enamel caries. In orthodontics it has been attributed to prolonged accumulation and retention of bacterial plaque on the enamel surface adjacent to the appliances. Plaque retention surrounding orthodontic appliances leads to enamel demineralization caused by organic acids produced by bacteria in the dental plaque. Plaque develops after the placement of a fixed appliance because the appliance often impedes the maintenance of good oral hygiene for the orthodontic patient. The components of the appliance create many new retention areas for microorganisms and impede proper access to the tooth surfaces for optimal cleaning. Decalcification may then occur. White spot formation represents a poor esthetic side effect of orthodontic treatment that may counteract the beneficial results of such treatment. Thus, prevention of white spot formation is important for the orthodontist to consider before, during and after orthodontic treatment for quality treatment result as professional."

"This research was carried out to study the difference in the antibacterial capacity of two kinds of filling materials, namely amalgam and composite resin, on S. mutans KPSK2 bacteria with different times of treatment. In total, 48 amalgam and composite resin samples each were prepared and then divided into four groups of treatment. Of each group, 6 samples were used to count the number of bacterial colonies and 6 samples to count the right obstacle zone. The results show that the best antibacterial capacity of composite resin occured within one week, while for amalgam the best performance appears within one day. The antibacterial capacity of flourine containing composites is stronger than that of amalgam for a time of 1 to 2 weeks."

"Pengurangan koloni Streptococcus mutans saliva pada anak setelah kumur larutan teh hijau 2,5%. Karies merupakan masalah dalam kedokteran gigi anak, dengan Streptococcus mutans sebagai bakteri dominan penyebab karies. Larutan teh hijau dan klorheksidin efektif mengurangi jumlah koloni dari Streptococcus .mutans. Namun belum diteliti lebih lanjut mengenai perbedaan efektivitas kedua bahan tersebut. Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan efektivitas berkumur dengan klorheksidin 0,2% dan larutan teh hijau 2,5% dalam menurunkan jumlah koloni Streptococcus mutans. Metode: Penelitian ini bersifat eksperimental semu dengan desain cross over. Jumlah sampel sebanyak 30 orang anak-anak berusia 6-12 tahun dengan indeks OHI-S kurang. Sampel saliva diambil masing-masing sebelum berkumur selama 3 detik dengan larutan klorheksidin 0,2% dan larutan teh hijau 2,5% dan 15 menit dilanjutkan 30 menit setelah berkumur. Sampel saliva dibawa ke laboratorium kemudian dilakukan perhitungan jumlah koloni bakteri Streptococcus mutans. Data dianalisis dengan menggunakan program SPSS versi 18.0 dengan uji ANOVA t-berpasangan, dan uji beda lanjut (LSD). Hasil: Uji ANOVA menunjukkan bahwa penurunan yang dihasilkan oleh masing-masing obat kumur merupakan penurunan yang signifikan. Hasil uji t-berpasangan menunjukkan tidak terdapat perbedaan jumlah koloni Streptococcus mutans yang signifikan antara klorheksidin dan larutan teh hijau pada interval waktu 15 menit dan 30 menit setelah berkumur. Simpulan: Tidak ada perbedaan yang bermakna antara klorheksidin dan larutan teh hijau terhadap jumlah koloni Streptococcus mutans.

---

Dental caries is a problematic area in pediatric dentistry, with Streptococcus mutans as the dominant bacterial cause. Green tea solution and chlorhexidine were effective in reducing the number of Streptococcus mutans. But study about the differences of these two materials has not been done. Objective: The aim of this study was to determine differences in the effectiveness of rinsing with 0.2% chlorhexidine and 2.5% green tea solution in reducing the number of Streptococcus mutans. Methods: This study is quase experimental study with cross-over design. Thirty children aged 6-12 years with poor OHI-S index were participated. Samples of saliva were taken respectively before rinsing for 3 seconds with 0.2% chlorhexidine solution and a solution of 2.5% green tea and 15 minutes followed 30 minutes after rinsing intervention. Saliva samples were taken to the laboratory then the number of Streptococcus mutans count were evaluated. The statistical analysis were performed by SPSS version 18.0 with ANOVA, t-paired and LSD test. Results: ANOVA test result showed that the decrease generated by each mouthwash is a significant reduction. T-paired test result showed that there is no difference in the number of Streptococcus mutans colonies significantly between chlorhexidine and green tea solution at 15 minutes dan 30 minutes after rinsing. Conclusion: There is no significant difference between chlorhexidine and green tea solution in reducing the number of Streptococcus mutans.;Pengurangan koloni Streptococcus mutans saliva pada anak setelah kumur larutan teh hijau 2,5%. Karies merupakan masalah dalam kedokteran gigi anak, dengan Streptococcus mutans sebagai bakteri dominan penyebab karies. Larutan teh hijau dan klorheksidin efektif mengurangi jumlah koloni dari Streptococcus .mutans. Namun belum diteliti lebih lanjut mengenai perbedaan efektivitas kedua bahan tersebut. Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan efektivitas berkumur dengan klorheksidin 0,2% dan larutan teh hijau 2,5% dalam menurunkan jumlah koloni Streptococcus mutans. Metode: Penelitian ini bersifat eksperimental semu dengan desain cross over. Jumlah sampel sebanyak 30 orang anak-anak berusia 6-12 tahun dengan indeks OHI-S kurang. Sampel saliva diambil masing-masing sebelum berkumur selama 3 detik dengan larutan klorheksidin 0,2% dan larutan teh hijau 2,5% dan 15 menit dilanjutkan 30 menit setelah berkumur. Sampel saliva dibawa ke laboratorium kemudian dilakukan perhitungan jumlah koloni bakteri Streptococcus mutans. Data dianalisis dengan menggunakan program SPSS versi 18.0 dengan uji ANOVA t-berpasangan, dan uji beda lanjut (LSD). Hasil: Uji ANOVA menunjukkan bahwa penurunan yang dihasilkan oleh masing-masing obat kumur merupakan penurunan yang signifikan. Hasil uji t-berpasangan menunjukkan tidak terdapat perbedaan jumlah koloni Streptococcus mutans yang signifikan antara klorheksidin dan larutan teh hijau pada interval waktu 15 menit dan 30 menit setelah berkumur. Simpulan: Tidak ada perbedaan yang bermakna antara klorheksidin dan larutan teh hijau terhadap jumlah koloni Streptococcus mutans."

"ABSTRAKSaliva merupakan zat eksokrin yang mengandung berbagai komponen, salah satunya adalah protein. Total protein dalam saliva dapat meningkat karena peningkatan aktifitas fisik, salah satu contohnya adalah aktifitas berlari. Di dalam rongga mulut, Streptococcus mutans merupakan mikroorganisme kariogenik yang memiliki peran penting dalam proses terjadinya karies. Tujuan: menganalisis perbedaan profil protein saliva yang diisolasi dari subjek pelari dan nonpelari sebelum dan setelah protein tersebut diinteraksikan dengan Streptococcus mutans ATCC 25175. Metode: sampel saliva unstimulated diambil dari 3 subjek pelari dan 3 subjek nonpelari. Identifikasi berat molekul protein saliva ditetapkan dengan menggunakan teknik SDS-PAGE dan pewarnaan commasie blue, sedangkan identifikasi interaksi protein saliva dengan Streptococcus mutans ditetapkan dengan menggunakan teknik SDS-PAGE, pewarnaan commasie blue dan Qubit Protein assay. Hasil: pada subjek pelari teridentifikasi protein dengan berat molekul sebesar 140 kDa, 100 kDa, 70 kDa, 50 kDa, 25 kDa, dan 15 kDa sedangkan pada subjek nonpelari teridentifikasi protein dengan berat molekul 70 kDa, 50 kDa, 25 kDa, dan 10 kDa. Interaksi protein saliva pelari dengan metode pewarnaan comassie blue tidak memvisualisasikan pita pada agar poliakrilamid sedangkan protein saliva subjek nonpelari terlihat pita sebesar 70 kDa. Interaksi protein saliva pelari dengan Streptococcus mutans dengan menggunakan Qubit Protein assay menunjukan konsentrasi sebesar 74,2 g/mL dan sebesar 93,2 g/mL pada saliva nonpelari. Kesimpulan: terdapat perbedaan profil dan berat molekul protein saliva pada subjek pelari dan nonpelari dan interaksi protein dengan Streptococcus mutans hanya tervisualisasikan pada protein saliva yang berasal dari subjek nonpelari.

---

ABSTRAKBackground Saliva is an exocrine substance containing various components, one of which is protein. The amount of total proteins in saliva may increase due to physical activity, namely running. In the oral cavity, Streptococcus mutans is a cariogenic microorganism that is vital in the forming process of caries. Objective Analyze the difference in salivary proteins profiles on subjects who are runners compared to non runners before and after the proteins are interacted with Streptococcus mutans ATCC 25175. Method Samples of unstimulated saliva were taken from 3 subjects who were runners and 3 subjects who were non runners. Identification of total molecular weight in salivary proteins was done using comassie brilliant blue color staining with the SDS PAGE technique. Identification of interaction in salivary proteins with Streptococcus mutans was also done using comassie brilliant blue color staining using SDS PAGE technique and Qubit Protein assay. Results In subjects who were runners, identification of molecular weight in the salivary protein results were 140 kDa, 100 kDa, 70 kDa, 50 kDa, 25 kDa, and 15 kDa while in non runners the identification of molecular weight in the salivary protein results were 70 kDa, 50 kDa, 25 kDa, and 10 kDa. Interaction of salivary proteins in runners using comassie blue coloring did not result in visualization of band on polyacrilamide agar while in non runners a band of 70 kDa was observed on polyacrilamide agar. Interaction of salivary proteins with Streptococcus mutans using Qubit Protein assay showed a concentration of 74.2 g mL in runners and 93.2 g mL in non runners. Conclusion There is a difference of proteins profile and molecular weight in subjects who were runners and non runners and proteins interaction with Streptococcus mutans is only visualized in the salivary proteins derived from non runners subject."

"Pendahuluan: protein saliva merupakan komposisi yang terkandung dalam salivadan berperan penting bagi keseimbangan ekosistem rongga mulut manusia. Total konsentrasiprotein saliva pada setiap individu bervariasi tergantung pada usia individu tersebut. Banyakdari protein saliva berfungsi untuk memproteksi rongga mulut dengan aktivitas antimikrobayang dimilikinya. Di sisi lain, saliva juga dapat mendukung pertumbuhan mikroorganismerongga mulut dengan membentuk pelikel. Streptococcus mutans bersama dengan pelikelsaliva berpartisipasi dalam adhesi bakteri di permukaan gigi. Selanjutnya mereka akanberkoordinasi sehingga membentuk dental plaque. Tujuan: menganalisis perbedaan massabakteri dan viabilitas Streptococcus mutans setelah pajanan protein saliva subjek anak dansubjek dewasa. Metode: sampel saliva subjek anak dan subjek dewasa dilakukan ujiBradford untuk mengetahui total protein saliva. Kemudian dilakukan perhitungan massabiofilm dengan uji crystal violet staining dan viabilitas bakteri dengan TPC. Setelah itudilakukan uji One-way Anova Hasil: Nilai signifikansi uji statistic menunjukan > 0,05sehingga tidak terdapat perbedaan bermakna massa bakteri maupun viabilitas bakteriStreptococcus mutans setelah pajanan protein saliva yang berasal dari subjek anak dan subjekdewasa secara statistik. Total konsentrasi protein saliva anak dan dewasa condong berbeda.Kesimpulan: Tidak terdapat perbedaan dampak pemajanan protein saliva asal subjek anakdan subjek dewasa terhadap pembentukan biofilm bakteri Streptococcus mutans ditinjau darimassa biofilm dan viabilitas bakteri.

---

Background: Salivary protein is the composition contained in saliva and plays an

important role in the balance of the human oral cavity ecosystem. The total salivary protein

concentration in each individual varies depending on the age of the individual. Many of

salivary proteins function to protect the oral cavity with their antimicrobial activity.

Therefore, saliva can also support the growth of oral microorganisms by forming pellicles

and as a source of nutrtion to bacteria. Streptococcus mutans together with the salivary

pellicle participate in the adhesion of bacteria on the tooth surface. Furthermore, they will

coordinate to form dental plaque. Objective: to analyze the differences in bacterial mass and

viability of Streptococcus mutans after the exposure of the salivary proteins from children

and adult subjects. Methods: Bradford test was used to determine the total of salivary protein

in saliva samples from children and adult subjects. The biofilm mass was calculated by using

crystal violet staining and bacterial viability by TPC. The distribution was analyzed using the

One-way Anova test Results: The p value of the statistical test shows > 0,05 so that there

were no significant difference in bacterial mass and viability of Streptococcus mutans after

exposure of salivary protein from children’s or adult’s saliva statisticaly. However, the total

salivary protein concentrations of children and adults tend to be different. Conclusion: There

was no differenece in the impact of salivary protein exposure from children’s and adult’s

saliva on the formation of Streptococcus mutans biofilm in terms of biofilm mass and

bacterial viability."

"ABSTRAKMutans streptococci are considered as major bacteria in human dental caries, and S. mutans and S. sobrinus are the ones most commonly found in humans. It has been shown from previous study that the numbers of S. sobrinus in oral samples are usually underestimated, and the S. sobrinus colonies are often misidentified as S. mutans. The aim of this study was to identify S. mutans and S. sobrinus from dental plaque of children. Dental plaque samples were collected using sterile cotton swabs from first and second upper deciduous molars from 3 children. Samples of dental plaque were inoculated onto MSB-0.5% yeast extract-20% sucrose. Identification of S. mutans and S. sobrinus was performed using examination of colony morphology and biochemical analysis with inulin and rafinose. Identification results were then documented as digital images with Olympus Digital BX 51. S. mutans form convex, translucent colonies with rough margins, while the S. sobrinus colonies are translucent, circular, with pinpoints are smooth margins. Aglisining bubble often accumulates on top of the colony when excessive glucan is synthesized from sucrose. Biochemical analysis had showed positive reaction on S. mutans, and negative on S. sobrinus. From this study it can be concluded that S. mutans and S. sobrinus could be identified clearly with examination of colony morphology and biochemical analysis."

"The study was a quasi experimental one, supported by microbilogical data. The respondents had been purposively chosen from three Bekasi elementary schools in 2002, with 72 children as treatment group and 72 children as control group. data collected included oral and laboratory examinations. Dental health status of permanent dentition was determined using DMF-T index. The data was collected at the start and 120 days after intervention. Data analysis was by paired t-test for before and after treatment, and by independent t-test for comparing treatment and control. For the average the means 2 samples t-test difference was used, and for the average difference between the same groups the mean one sample t-test was applied. For the nominal scale proportion difference the likelihood ratio test was applied, and for the ordinal scale the nonparametric one-way Anova was used. Simple regression test was used to determine the relation between dependent and independent variables. The results of the study show decrease of S. mutans colonies in saliva after treatment (p= 0,001) and decreasing DMF-T score for treatment group. In control group, there is no decrease in S. mutans colonies, but increasing DMF-T score was found. The conclusion was that glass ionomer cement fillings can inhibit S. mutans growth in saliva."