"Latar belakang: ECC menjadi masalah serius di Indonesia dan Dunia. Terdapat 3
komponen ECC, yaitu gigi, mikroba, serta lingkungan rongga mulut yang dalam hal ini
yaitu protein saliva. Penyebab dari ECC sendiri yaitu bakteri Streptococcus mutans.
Tidak hanya itu, Candida albicans sering dihubungkan dengan Streptococcus mutans
pada plak ECC. Namun, adanya riset di mana Candida albicans cenderung mengurangi
sifat kariogenik Streptococcus mutans menarik untuk diteliti. Tujuan: menganalisis
peran protein saliva ECC terhadap pertumbuhan biofilm Streptococcus mutans dan
Streptococcus mutans dan Candida albicans (atau dual-spesies) di rongga mulut.
Metode: Setiap sampel dilakukan uji SDS-Page untuk melihat apakah terdapat
perbedaan profil protein antar setiap sampel. Lalu, sampel dilakukan pengenceran
menjadi 3 konsentrasi, kemudian diinkubasi bersama dengan Streptococcus mutans
serta dual-spesies di dalam 96-well plate selama 24 jam dan 48 jam secara anaerob.
Lalu, masing-masing biofilm dilakukan uji Crystal Violet Staining (untuk mendapatkan
nilai Optical density) serta Total Plate Count. Hasil: Tidak terdapat perbedaan profil
protein antara saliva ECC dengan laju alir saliva <30 detik, 30-60 detik, 30-60 detik
bebas ECC. Pada variabel konsentrasi protein, terdapat perbedaan dan kenaikan nilai
rerata pada nilai Optical density biofilm pada Streptococcus mutans dan dual-spesies.
Tidak terdapat perbedaan secara statistik antara konsentrasi protein saliva dengan
viabilitas mikroba pada biofilm Streptococcus mutans dan dual-spesies meski nilai
rerata menunjukkan penurunan viabilitas mikroba. Pada biofilm Streptococcus mutans
dan dual-spesies, tidak terdapat perbedaan bermakna pada hasil uji Optical density dan
viabilitas mikroba berdasarkan variabel waktu inkubasi biofilm. Meski nilai rerata
menunjukkan adanya penurunan pada Optical density Streptococcus mutans, kenaikan
pada viabilitas mikroba Streptococcus mutans, dan kenaikan pada Optical density
sekaligus viabilitas mikroba dual-spesies, namun tidak memengaruhi nilai
komparasinya. Kesimpulan: Protein saliva dapat memengaruhi pembentukan biofilm
baik Streptococcus mutans maupun kombinasi dual-spesies Streptococcus mutans
dengan Candida albicans. Waktu inkubasi biofilm tidak dapat memengaruhi
pembentukan biofilm Streptococcus mutans maupun kombinasi dual-spesies
Streptococcus mutans dengan Candida albicans
Background: ECC is a serious problem in Indonesia and the world. There are 3
components of ECC, namely teeth, microbes, and the oral environment, in this case
salivary protein. The cause of ECC itself is Streptococcus mutans. Not only that,
Candida albicans is often associated with Streptococcus mutans in ECC plaques.
However, the research in which Candida albicans tends to reduce the cariogenic
properties of Streptococcus mutans is interesting. Purpose: to analyze the role of the
ECC salivary protein on the growth of Streptococcus mutans and combination of
Streptococcus mutans and Candida albicans (or dual-species) biofilms in the oral cavity.
Methods: Each sample was subjected to an SDS-Page test to see if there were
differences in protein profiles between each sample. Then, the sample was diluted into 3
concentrations, then incubated together with Streptococcus mutans and dual-species in
96-well plates for 24 hours and 48 hours anaerobically. Then, each biofilm was
subjected to a Crystal Violet Staining test (to obtain Optical density value) and Total
Plate Count. Results: There was no difference in protein profile between salivary ECC
with salivary flow rates <30 seconds, 30-60 seconds, ECC-free 30-60 seconds. In the
protein concentration variable, there were differences and an increase in trend lines in
the Optical density value of biofilms in Streptococcus mutans and dual-species. There
was no statistical difference between salivary protein concentrations and microbial
viability in Streptococcus mutans and dual-species biofilms, although the trend line
showed a decrease in microbial viability. In Streptococcus mutans and dual-species
biofilms, there were no significant differences in the Optical density test results and
microbial viability based on the biofilm incubation time variables. Although the trend
line showed a decrease in Optical density Streptococcus mutans, an increase in
microbial viability of Streptococcus mutans, and an increase in Optical density as well
as dual-species microbial viability, it did not affect the comparative value. Conclusion:
Salivary protein can influence biofilm formation for both Streptococcus mutans and the
dual-species combination of Streptococcus mutans and Candida albicans. Biofilm
incubation time could not affect the biofilm formation of both Streptococcus mutans
and the dual-species combination of Streptococcus mutans and Candida albicans"
Depok: Fakultas Kedokteran Gigi Universitas Indonesia, 2021
