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"ABSTRAKTelah dilakukan penelitian mengenai pemanfaatan teknik adaptasi danimobilisasi sel khamir Candida tropicalis InaCC Y799 pada fermentasi xilitol darihidrolisat hemiselulosa daun tebu (Saccharum officinarum L.). Praperlakuan dauntebu dilakukan menggunakan 1,8% asam maleat dan iradiasi gelombang mikropada suhu 180 C selama 5 menit. Penelitian bertujuan untuk mempelajarikemampuan khamir untuk tumbuh dan beradaptasi pada hidrolisat hemiselulosasebelum fermentasi, dan meneliti potensi penggunaan matriks kalsium alginatuntuk imobilisasi sel khamir, serta pengaruhnya dalam peningkatan produksixilitol dari hidrolisat hemiselulosa daun tebu. Hasil menunjukkan bahwa khamirC. tropicalis InaCC Y799 teradaptasi mampu tumbuh pada media hidrolisathemiselulosa daun tebu. Adaptasi khamir pada 75% hidrolisat menghasilkankonsentrasi dan rendemen xilitol maksimum masing-masing sebesar 11,27 1,65g/L dan 0,56 0,05 g/g (54,98% dari nilai rendemen teoritis) selama 24 jamfermentasi, lebih tinggi daripada khamir tidak teradaptasi (kontrol). Namundemikian, imobilisasi C. tropicalis pada kalsium alginat hanya menghasilkankonsentrasi dan rendemen xilitol maksimum masing-masing sebesar 5,51 0,63g/L dan 0,27 0,04 g xilitol/g xilosa awal (29,97% dari rendemen teroritis)selama 48 jam fermentasi. Konsentrasi dan rendemen xilitol pada sistem selterimobilisasi setengah kali lebih rendah daripada sel bebas (kontrol).

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ABSTRACTResearch on adaptation and immobilization method of Candida tropicalisInaCC Y799 in xylitol production from sugarcane (Saccharum officinarum L.)waste hemicellulosic hydrolysate has been conducted. Sugarcane waste werepretreated with 1,8% of maleic acid and microwave at 180 C for 5 minutes. Theaim of this research were to study the effects of yeast adaptation using sugarcanewaste hydrolysate and the potential of using calcium alginate as immobilizationmatrix of yeast C. tropicalis InaCC Y799 in the xylitol production duringfermentation. The results revealed that fermentation using adapted yeast in 75%concentration of hydrolysate produce higher xylitol concentration and yield thanthose with non adapted yeast. The highest xylitol concentration and yield obtainedusing adapted yeast were 11.27 1.65 g/L and 0.56 0.05 g xylitol /g initialxylose (54.98% of theoretical yield) for 24-hours fermentation. However, thehighest xylitol yield obtained by immobilization method were 5.51 0.63 g/L and0.27 0.04 g xilitol/g initial xylose (29.97% of theoretical yield) for 48-hoursfermentation, which were lower than free cells system."

"Indonesia has wide palm oil plantation which produce Empty Fruit Bunch (EFB) waste around 32 million tons per year. EFB is a potential material for bioethanol through pretreatment, saccharification, and fermentation. Fermentation has important role in bioethanol production because this process will convert glucose into ethanol. The most common microorganism used in fermentation process is Saccharomyces cerevisiae. But, the use of S. cerevisiae in bioethanol fermentation using lignocellulosic hydrolysate have a problem that microorganisms cannot grow well. This is due to the presence of inhibitor in the hydrolysate. Solution for this problem is using S. cerevisiae which cultivated on hydrolysate media that will be used in the fermentation (in this case EFB). This research will investigate cultivation of S. cerevisiae on EFB hydrolysate, to obtain the optimum operating conditions such as aeration and nutrients. Fed-batch system is used for cultivation. Optimum condition are determined after analyzing cell number and ethanol yield from dried S. cerevisiae. Optimum condition for cultivation are 1 v/v per min aeration and glucose 5 g/L which produce ethanol yield 24%. We also scale-up the dried yeast into 43.7 g and need a cost Rp 19,958/g which is more expensive than commercial yeast."

"Mikroalga Nannochloropsis sp. memiliki kandungan biomassa bervariasi, salah satunya protein. Hidrolisis protein menghasilkan peptida dan asam amino sehingga meningkatkan bioaktivitas dari protein. Penelitian ini bertujuan untuk mendapatkan protein hidrolisat dari mikroalga Nannochloropsis sp. serta mengetahui keamanan dan efektivitasnya sebagai produk kosmetik anti-aging. Protein hidrolisat mikroalga Nannochloropsis sp. (PHMN) diperoleh dari proses hidrolisis menggunakan enzim alkalase. PHMN dievaluasi perolehan rendemen, derajat hidrolisis, kandungan proksimat, asam amino serta aktivitas anti-elastasenya. PHMN diformulasikan menjadi sediaan emulgel, kemudian dievaluasi sifat fisikokimia dan stabilitasnya. Uji keamanan produk kosmetik PHMN dilakukan dan efektivitas produk kosmetik PHMN sebagai anti-aging dievaluasi dengan mengukur serat kolagen, elastisitas, dan kelembaban kulit relawan. Protein hidrolisat yang diperoleh memiliki nilai rendemen sebesar 25,77%±3,16% (b/b), derajat hidrolisis sebesar 36,73%, dan mengandung asam amino yang didominasi oleh asam glutamat, asam aspartat, dan leusin. Nilai konsentrasi penghambatan setengah maksimal (IC50) PHMN sebagai anti-elastase yaitu 244,43 mg/mL. Produk kosmetik PHMN menunjukkan stabilitas yang baik yaitu homogen dan kadar yang stabil setelah 24 minggu. Penggunaan produk kosmetik PHMN tidak menyebabkan iritasi dan meningkatkan serat kolagen, elastisitas, dan kelembaban kulit setelah 28 hari. Dari penelitian ini disimpulkan bahwa diperoleh protein hidrolisat dari mikroalga Nannochloropsis sp. serta produk kosmetik PHMN memiliki karakteristik yang baik, aman, dan efektif sebagai anti-aging

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Microalgae Nannochloropsis sp. contain various biomass composition including protein. Protein hydrolysis generate peptide and amino acids so that protein bioactivity improves. The purpose of study was to obtain protein hydrolysate from Nannochloropsis sp. microalgae, also assess its safety and efficacy as anti-aging cosmetic product. Nannochloropsis sp. protein hydrolysate (NPH) was obtained from microalgae through enzymatic hydrolysis using alcalase enzyme and characterized for yield, degree of hydrolysis (DH), proximate content, amino acids composition, and anti-elastase activity. NPH was formulated as emulgel, then evaluated for physical characteristics and stability. NPH cosmetic product was evaluated for safety and efficacy as anti-aging by measuring collagen fibers, elasticity, and moisture in volunteers. NPH was obtained with yield of 25.77±3.16% (w/w), DH value of 36.73%, and amino acids dominated by glutamic acid, aspartic acid, and leucin. Half-maximum inhibitory concentration (IC50) value as anti-elastase was 244.43 µg/mL. NPH cosmetic product showed good stability which homogenous and had stable protein content after 24 weeks storage. NPH cosmetic product usage did not cause skin irritation and increased collagen fiber, elasticity and moisture after 28 days. In conclusion, protein hydrolysate was obtained from Nannochloropsis sp. microalgae and NPH cosmetic product had good characteristic, safe, and effective as anti-aging."

"Bioetanol muncul sebagai alternatif yang menjanjikan sebagai pengganti bahan bakar fosil, karena potensi sumber daya hayati (biomassa) yang cukup besar di Indonesia. Prosesnya sederhana dengan melakukan fermentasi biomassa menggunakan mikroorganisme penghasil etanol, seperti Saccharomyces cerevisiae. Hasil kultivasi secara umum kurang memberikan hasil yang memuaskan ketika fermentasi, khususnya pada hidrolisat lignoselulosa. Hal ini dapat diatasi dengan kultivasi menggunakan hidrolisat tersebut sehingga ketahanan khamir terhadap inhibitor bertambah. Media yang digunakan adalah hidrolisat tandan kosong sawit karena merupakan biomassa lignoselulosa yang mudah ditemukan di Indonesia. Kultivasi dilakukan secara batch untuk mendapatkan kondisi aerasi dan nutrisi optimal dengan cara menganalisis konsentrasi sel yang dihasilkan serta uji coba fermentasi etanol S. cerevisiae yang telah dikeringkan. Didapatkan kondisi optimal aerasi 1 v/v per menit dengan penambahan glukosa 5 g/L dengan yield etanol sebanyak 24%. Scale-up produksi didapatkan produk sebanyak 43,7 gram dengan biaya Rp 19.958,00 per gram.

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Bioethanol emerges as a promising alternative to replace fosil-based fuel because of the biomass potency in Indonesia. The process in making it is rather simple, which is by fermenting biomass using ethanol producing microorganisms like Saccharomyces cerevisiae. A problem arises when conventional cultivated S. cerevisiae are used to ferment, especially on hydrolysate from lignocellulosic biomass, where they do not give a satisfying result. This can be solved by cultivating using the same hydrolysate used for fermentation so that the yeast can be more resistant towards inhibitors from the hydrolysate. The medium used is empty fruit bunch since it is easily found in Indonesia. Batch system is used for cultivation to get optimal aeration and nutrition condition by analyzing cell number and ethanol yield from dried S. cerevisiae. Condition of 1 v/v per minute aeration and added glucose 5 g/L with ethanol yield 24% is found to be most effective. Production scale-up resulted to 43,7 gram of dried yeast with cost Rp 19.958,00 per gram."

"Furfural merupakan komponen yang banyak digunakan pada industri farmasi, resin, hingga pengolahan minyak bumi dengan permintaan dan harga yang terus meningkat setiap tahunnya. Purifikasi furfural dengan metode ekstraksi cair-cair lebih berpotensi untuk dikembangkan dibandingakan metode distilasi karena prosesnya dipandang lebih berkelanjutan. Penelitian ini bertujuan meningkatkan perolehan furfural dengan metode ekstraksi cair-cair pada larutan model hidrolisat TKKS. Pada penelitian ini digunakan beberapa pelarut dengan afinitas tinggi terhadap furfural yaitu 1-butanol, MIBK, dan toluena, pada variasi suhu ekstraksi 65°C, 85°C, dan 105°C, serta variasi suhu ekstraksi 30, 60, dan 90 menit. Penentuan kadar furfural pada ekstrak dilakukan dengan HPLC. Optimasi kondisi operasi ekstrasi dilakukan dengan Response Surface Methodology RSM) untuk mendapatkan nilai parameter optimum tiap variabel dan mempelajari pengaruh suhu ekstraksi, waktu ekstraksi, dan pemilihan pelarut organik terhadap besar perolehan furfural. Kondisi operasi optimum ekstraksi cair-cair furfural didapatkan pada suhu 105°C selama 30 menit menggunakan pelarut MIBK, dengan perolehan furfural sebesar 49,103%. Penelitian ini berhasil mendapatkan kondisi optimum yang memberikan perolehan furfural tertinggi dengan kondisi ekstraksi cair-cair furfural dari model hidrolisat TKKS yang paling efisien

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Furfural is a compound that is widely used in the pharmaceutical, resins, and petroleum industry with continuous increase of price and demand every year. Liquid-liquid extraction is a purification method with great potential as a more sustainable option for furfural purification compared to distillation method. This research aimed to increase furfural yield from OPEFB hydrolysate model using liquid-liquid extraction method. In this research, solvent with high affinity towards furfural such as 1-butanol, MIBK, and toluene were used as solvent at extraction temperature of 65°C, 85°C, and 105°C, with extraction time of 30, 60, and 90 minutes. Determination of furfural concentration in the extract is carried out using HPLC. Optimization of the operating condition is done with Response Surface Methodology (RSM) to obtain optimum value of each parameter and investigate the effects of extraction time, temperature, and solvent selection on furfural yield. The optimum operating condition for furfural liquid-liquid extraction is obtained at 105°C for 30 minutes using MIBK as solvent, which resulted in 49.103% furfural yield. This research has provided the most efficient liquid-liquid extraction condition that produces the highest yield of furfural from OPEFB hydrolysate model."