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Abstrak :
Telah dilakukan penelitian penetapan kadar deksametason dalam sediaan tablet campuran dengan CTM secara spektrofotometri dengan pereaksi INH dan secara spektrofotodensitometri. Pada penetapan kadar deksametason secara spektrofotometri dengan pereaksi INH, terjadi reaksi antara deksametason dengan INH menghasilkan senyawa hidrazon yang berwarna kuning senyawa inilah yang diukur secara spektrof otometri., Sedangkan padaspek. trofotodensitometri deksametason pada lempeng KLT pada daerah panjang gelombang ultra violet memberikan fluoresensi, hasil fluoresensi inilah yang diukur secara spektrofotodensitometri. Hasil penelitian menunjukkan bahwa kedua metode penetapan kadar deksametason tersebutmemberikan hasil yang cukup baik dan reprodusibel dan tidak- memberikan perbedaan yang bermakna secara statistik. ......A study on quantitative assay of dexamethasone in tablet mixed with CTM spectrbfotometrically with INK as reagent and with spectrofotodensitometry had been done. On quantitative assay of dexamethasone spectrofotometrically with INH as reagent, there occurs a reaction between dexamethasone with INH producing hydrazone, yellow in color, this compound is measured spectrofotometrically. While on spectrofotodensitometry dexamethasone on. TLC sheet at ultra violet region gives fluorescence, this fluorescence is mea sured spectrofotodensitometry.. The result of the study, showed that the two method of quantitative assay of dexamethasone, gave quite good result and reproducible and did not give difference significantlystatistically.

Abstrak :
Latar Belakang: Data GLOCOBAN tahun 2018 menunjukkan bahwa kanker lambung merupakan penyebab kematian akibat kanker nomor tiga di dunia. Hingga saat ini, belum terdapat deteksi dini untuk kanker lambung. Kanker lambung sering ditemukan dalam kondisi yang sudah berat, karena 25,8% kasus tidak terdiagnosis ketika dilakukan upper endoscopy. Sejumlah penelitian menunjukkan bahwa spektrofotometri dapat digunakan dalam mendeteksi jaringan kanker, antara lain spektroskopi Raman dan optik. Hingga saat ini belum ada penelitian yang mendeteksi jaringan kanker lambung berdasarkan spektrofotometri sederhana. Tujuan: Studi ini dilakukan untuk mengetahui ambang batas perbedaan panjang gelombang reflektansi pada jaringan kanker normal dengan jaringan pra-kanker dan jaringan kanker lambung serta menganalisis akurasi spektrofotometer dalam klasifikasi jaringan.. Metode: Reflektansi jaringan mencit Mus musculus diukur menggunakan spektrofotometer konvensional pada panjang gelombang 431.5-705.2 mm. Hasil reflektansi kemudian digunakan dalam model machine learning untuk menentukan klasifikasi berdasarkan reflektansi. Hasil: Machine learning Tree menggunakan panjang gelombang 431,5, 494,2, dan 502.5 nm. Analisis Principal Component Analysis menunjukkan adanya penumpukkan antara jaringan prekanker dengan jaringan kanker. Metode Random Forest (CA: 0.857, precision: 0.872, recall: 0.857) lebih baik dalam mengklasifikasikan jaringan kanker lambung dibandingkan metode Tree (CA;0,607, precision:0,619, dan recall:0,607) dan logistic regression (CA:0,750, precision: 0,739, dan recall:0,750). Spektrofotometri reflektans sederhana memiliki sensitivitas sebesar 68.42%-89.47% dan spesifisitas sebesar 44-88.89% dalam mendeteksi jaringan pra-kanker dan jaringan kanker. Kesimpulan: Dengan rentang panjang gelombang 431,5, 494,2, dan 502.5 nm, spektrofotometri sederhana tidak dapat membedakan jaringan pra-kanker dan kanker karena terdapat penumpukan protein seperti miglobin, dan juga tingkat sensitivitas dan spesifisitas yang baik dalam membedakan jaringan normal dan tidak normal. ......Background: GLOCOBAN in 2018 shows that gastric cancer is the third leading cause of death for cancer-related disease. Until now, there’s no early detection for gastric cancer. This causes gastric cancer to be diagnosed at a later stage, because 25,8% gastric cancer cases are undiagnosed even with upper endoscopy 3 years before diagnosis.A number of study has shows that spectrophotometry can be used for detecting gastric cancer, such as Raman spectroscopy and optical. Until now, there is no research that detect gastric cancer using conventional spectrophotometer. Objectives This study aims to understand the difference between wavelength of the reflectance from the normal gastric tissue, precancerous gastric tissue, and gastric cancer tissue and analyze the accuracy of conventional spectrophotometer to classify the tissues. Methods The reflectance of the tissue of Mus musculus is evaluated using conventional spectrophotometer at the wavelength of 431.5-705.2 mm. The resulting data will then be used in a machine learning model to help classify the tissue based on the reflectance Result: Wavelengths used by Tree is 431,5, 494,2, dan 502.5 nm. Analysis using Principal Component Analysis shows a grouping formed by the gastric precancer tissue and gastric cancer tissue. Random Forest (CA: 0.857, precision: 0.872, recall: 0.857) is proven to be better for classifying the tissue based on the reflectance compared to Tree (CA;0,607, precision:0,619, and recall:0,607) and Logistic regression (CA:0,750, precision: 0,739, and recall:0,750). Conventional reflectance spectrophotometry yielded a 68.42%-89.47% sensitivity and 44-88,89% specificity in differentiating normal gastric tissue with abnormal gastric tissue. Conclusion: Within the wavelength of 431,5, 494,2, dan 502.5 nm, conventional spectrophotometer cannot differentiate precancerous lesion with gastric cancer tissue due to the abundance of protein such as myoglobin, and having a good sensitivity and specificity in differentiating normal and abnormal tissue.

Abstrak :
ABSTRAK
Asam lipoat adalah senyawa yang terkandung dalam intra dan ekstraselular yang bertindak sebagai koenzim piruvat dehidrogenase, penangkal racun, pengkat logam berat dan antioksidan. Pengukuran asam lipoat diperlukan untuk mengetahui jumlah asam lipoat yang menjalankan fungsinya baik sebagai koenzim atau antioksidan. Tetapi pengukuran ini membutuhkan alat khusus seperti HPLC dan proses yang tidak mudah. Alat yang umum dan mudah seperti spektrofotometer diharapkan bisa menjadi alat penentuan asam lipoat. Pengukuran asam lipoat menggunakan spektrofotometri dengan metanol UV dan PdCl2 terlihat telah diuji dan diperoleh hasil yang valid dan dapat diandalkan dalam sediaan obat atau farmasi tetapi belum diuji untuk plasma. Pengukuran asam lipoat dalam plasma dan leukosit menggunakan PdCl2 menghasilkan pengukuran yang valid, dengan akurasi, presisi tinggi dan tidak berbeda dengan pengukuran asam lipoat menggunakan HPLC, p= 0,99. Sedangkan UV methanol berbeda dibanding HPLC p= 0,0001 atau tidak valid.

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ABSTRACT
Lipoic acid is a compound contained in intra and extracellular that act as a coenzyme of Pyruvate Dehydrogenase, antidote, chelating agent and antioxidant. Measurement of lipoic acid is needed to determine the amount of lipoic acid that performs its functions either as a coenzyme or an antioxidant. But this measurement requires a special tool such as High Performance Liquid Chromatography HPLC and a process that is not easy. A common and easy tool such as a spectrophotometer is expected to be a tool of lipoic acid determination. Measurement of lipoic acid using spectrophotometry with UV methanol and visible PdCl2 has been tested and obtained valid and reliable results in drug or pharmaceutical preparations but not yet tested for plasma. Determination of lipoic acid in plasma and leukocytes using PdCl2 produced valid result, with high accuracy, precision and was not different from lipoic acid measurement using HPLC, p 0.99. While UV methanol was different compare to HPLC p 0.0001 or was not valid.

Abstrak :
ABSTRACT
It had been done a study on the assay and potency of three samples of Tetracycline Hydrochloride visually having different colours. The study on the assay of the three samples of Tetracycline Hydrocloride was done by using pectrophotometric method in alkaline media, while the study on their potency was done by using turbidimetric method with latin square pattern. The result obtained are as follows: - The change in colour that is significant in samples of Tetra cycline Hydrochloride examined, does not affect their contents or potency. - The spectrophotometric method has precision difference in-. significantly compared with turbidimetric one. With respect to the above results it is still necessary to look for the correlation between the amount of the content of anhydrotetracycline and its effect on spectrophotometric and turbidimetric method.