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ABSTRAK
Latar belakang: Ketahanan ikatan resin komposit-dentin merupakan salah satu penentu keberhasilan restorasi resin komposit. Tujuan penelitian ini adalah menganalisis efek klorheksidin terhadap degradasi kekuatan ikat resin kompositdentin. Metode: Dua puluh empat sampel dentin yang diambil dari mahkota gigi premolar, dibagi menjadi tiga kelompok yang diberikan perlakuan berbeda. Kelompok I diberi perlakuan bahan bonding tanpa klorheksidin, kelompok II diberi perlakuan klorheksidin dan bonding, kelompok III diberi perlakuan bonding mengandung klorheksidin, pada tiap kelompok dibagi menjadi 2 sub-kelompok yaitu kelompok tanpa direndam dan kelompok yang direndam NaOCl 10% selama satu jam, sehingga didapat enam sub-kelompok. Kemudian seluruh kelompok di ukur kekuatan ikat gesernya menggunakan Universal Testing Machine. Satu sampel dari setiap sub-kelompok dilakukan Scanning Electron Microscope (SEM). Data dianalisa statistik dengan uji hipotesis Kruskal Wallis yang dilanjutkan dengan uji Mann-Whitney. Hasil: rerata kekuatan ikat geser sebelum perendaman NaOCl 10% tertinggi kelompok I sedangkan rerata kekuatan ikat geser setelah perendaman NaOCl 10% tertinggi pada kelompok III. Terdapat perbedaan bermakna antara kelompok I terhadap kelompok II dan antara kelompok II terhadap kelompok III. Kesimpulan: Klorheksidin mempunyai efek terhadap pengurangan degradasi kekuatan ikat geser resin komposit-dentin.

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ABSTRACT
Background: Resilience of composite resin-dentin bonding known as one of success composite resin restoration determinants. The purpose of this study was to analyze the effect of chlorhexidine on reducing the degradation of composite resin-dentin shear bond strength. Methods: Twenty-four premolar crowns were divided into three groups then given different treatments. Group I was treated material bonding without chlorhexidine, group II was treated with chlorhexidine and bonding, group III was treated with chlorhexidine-contained bonding. Each group was divided into two sub-groups: the group without immersion of NaOCl 10% and the group with immersion of NaOCl 10% for one hour, then it were obtained six sub-groups. After twenty-four hours, shear bond strengths measured using Universal Testing Machine. A sample of each group was photographed with Scanning Electron Microscope (SEM). Statistical analysis was done by Kruskal Wallis test, then followed by Mann Whitney test to determine significance between groups. Results: The mean value of shear bond strength before immersion of NaOCl 10% was highest on Group I, while the mean value of shear bond strength after immersion of NaOCl 10% was highest on Group III. There are significant differences between Group I with Group II and between Group II with Group III. Conclusion: Chlorhexidine have an effect on reducing the degradation of shear bond strength of resin-dentin bonding

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Latar Belakang: Penelitian mengenai pengaruh pelebaran korona saluran akar terhadap tingkat ketepatan apex locator hanya sedikit. Tujuan: Membandingkan ketepatan lokasi foramen apikalis pada dua tipe apex locator dan menilai pengaruh pelebaran koronal saluran akar. Metode: Panjang kerja 16 gigi premolar satu mandibula diukur secara visual, menggunakan dua jenis apex locator saat sebelum dan setelah dilakukan pelebaran korona saluran akar. Hasil: Berbeda bermakna ketepatan pada tiap jenis apex locator setelah dilakukan pelebaran korona, namun tidak berbeda bermakna pada keduanya setelah pelebaran koronal saluran akar. Kesimpulan: pelebaran korona saluran akar meningkatkan ketepatan apex locator mengukur lokasi foramen apikalis.

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Background: Only a few studies on the effect of coronal preflaring to the accuracy of apex locator. Objective: To compare the accuracy of two types of apex locators and evaluate the influence of coronal preflaring in them. Method: working length of sixteen first mandibular bicuspids were measured visually, using two types apex locator at before and after coronal preflaring. Result: statistically significant of two type apex locators at before and after coronal preflaring, no statistically significant at both types after coronal preflaring. Conclusion: coronal preflaring will increase the accuracy of apex locator in locating the apical foramen.

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Latar Belakang: Ekstrak biji anggur (GSE) mengandung 74-78% proantosianidin yang berfungsi sebagai pengikat silang kolagen. Tujuan: menganalisis kemampuan GSE sebagai larutan irigasi saluran akar dalam membersihkan smear layer pada daerah sepertiga apeks. Metode: lima puluh gigi dibagi menjadi lima kelompok. Kelompok 1 meggunakan aquades, kelompok 2 menggunakan GSE 3.25%, kelompok 3 menggunakan GSE 6.5%, kelompok 4 menggunakan GSE 13% dan kelompok 5 menggunakan EDTA 17%. Kemudian dilakukan pemeriksaan tingkat kebersihan sepertiga apeks menggunakan SEM dan diberi skor. Analisis data menggunakan uji kolmogorov-smirnov. Hasil: Skor 0 terbanyak pada GSE 13% (40%), skor 1 terbanyak pada GSE 6.5% (70%) dan skor 2 terbanyak pada aquades (80%). Kesimpulan: Larutan ekstrak biji anggur mempunyai potensi dapat membersihkan smear layer pada daerah sepertiga apeks dinding saluran akar. ......Background: Grape seed extract (GSE) consisting of 74-78% proanthocyanidin, is a cross linking agent. Purpose: investigate the ability of GSE in removing smear layer on apical third of root canal wall. Materials and Method: fifty five extracted incisors were divided into 5 groups. Grup 1 used aquadest, group 2 used 3.25% GSE, group 3 used 6.5% GSE, group 4 used 13% GSE and group 5 used 17% EDTA. The cleanliness of smear layer were evaluated by SEM and scored. The data were analyzed using kolmogorov-smirnov test. Results: Score 0 maximum in group GSE 13% (40%), score 1 maximum in group GSE 6.5% (70%) and score 2 maximum in group aquadest (80%). Conclusion: Grape seed extract solution has a potential to remove smear layer on apical third of root canal wall.

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Latar Belakang: Restorasi resin komposit masih memiliki kekurangan, yaitu terjadinya kebocoran mikro akibat kontraksi saat polimerisasi sehingga dapat menyebabkan kegagalan restorasi. Penelitian ini bertujuan untuk menganalisis perbedaan tingkat kebocoran mikro dinding restorasi kelas I antara RK packable (RP) dan RK flowable dengan kandungan filer tinggi (RF). Metode: Kavitas kelas I dipreparasi pada tiga puluh dua gigi premolar kemudian dibagi menjadi dua kelompok. Kelompok pertama ditumpat dengan RP, kelompok kedua dengan RF, keduanya ditumpat secara inkremental. Selanjutnya spesimen dilakukan uji thermocycling dan diikuti perendaman dalam biru metilen 1% selama 24 jam. Gigi kemudian dibelah bukolingual dan diamati menggunakan mikroskop stereo pembesaran 14x dan dinilai dalam skala ordinal (0-4). Analisis statistik dilakukan dengan uji Kolmogorov-Smirnov. Hasil: Tidak terdapat perbedaan yang signifikan antara Kelompok RP dan RF (p=0,699). Kesimpulan: Tidak terdapat perbedaan bermakna antara kebocoran mikro menggunakan RP maupun RF yang ditumpat secara inkremental. Namun secara substansi, RF menunjukkan kebocoran mikro lebih sedikit dibandingkan dengan RP. ......Background: Composite resins undergo contraction during polymerization which may result in microleakage and leads to restoration failure. The purpose of this study is to analyze the microleakage of Class I restorations that were filled with packable composite (RP) and high filler flowable composite (RF) incrementally. Methods: Standardized Class-I cavities were prepared on 32 extracted human premolars and randomly assigned into two groups. The first group were filled with RP and the second group were filled with RF. The specimens were subjected to thermocycling, followed by immersion in 1% methylene blue dye for 24 hours. The teeth were sectioned bucco-ligually and evaluated for microleakage under 14x magnification stereomicroscope and scored in ordinal scale (0-4). Statistical analysis was performed with the Kolmogorov-Smirnov test. Results: There was no significant difference between group RP and RF (p=0.699). Conclusion: There is no significance difference between microleakage by RP and RF. But substantially, RF provided less microleakage than RP.

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Tesis ini membahas mengenai bagaimana tingkatan sensualitas pada suatu iklan yang dicerminkan dalam high, medium, dan low sensuality serta penggunaan model sensual tunggal (single) dan berpasangan (couple) berpengaruh pada variabel-variabel yang mengacu pada cognitive response model yang terdiri dari variabel source thought, message thought, ad execution thought, attitude toward brand (Ab), attitude toward ad (Ad), dan purchase intention. Penelitian ini merupakan penelitian eksperimen yang dilakukan dengan pemisahan kelompok melalui factorial design 3x2 (tiga tingkat sensualitas dan dua jenis penggunaan model). Dengan jumlah total responden 210 responden yang keseluruhannya adalah wanita dengan 35 reponden pada masing-masing sel, diketahui adanya perbedaan rata-rata yang signifikan terhadap Ab, Ad, dan minat beli dengan keterangan bahwa semakin rendah tingkat sensualitas maka semakin tinggi rata-rata yang diperoleh untuk ketiga variabel tersebut. Sementara itu untuk hasil perbandingan rata-rata antara model single dan couple banyak variabel yang dinyatakan tidak berbeda secara signifikan. Hal ini dikarenakan penggunaan model mengarah pada bagian dari source yang merupakan variabel kognitif. Sementara itu diketahui bahwa pengaruh variabel-variabel kognitif terhadap variabel sikap tidak mempunyai pengaruh yang cukup besar apabila dibandingkan pengaruh sikap terhadap minat beli untuk kasus iklan dengan sensual appeals menurut sudut pandang wanita. ......This research discusses how the sensuality level in an advertisement which is reflected in high, medium, and low sensuality along with the utilization of single and couple sensual model affects the variables, which refer to cognitive response model consisting of source thought, message thought, ad execution thought, attitude toward brand (Ab), attitude toward ad (Ad), and purchase intention. This is an experimental research which is applying group separation through factorial design 3x2 (three levels of sensuality and two types of model utilization). After receiving answers from 35 respondents in each cell, the research found that there is an average significant difference toward Ab, Ad, and purchase intention, with additional information that the lower the sensuality levels, the higher the average value to the three variables mentioned above. Furthermore, the comparison between the single and couple model shows that there are many variables which are not significantly different. This is caused by the model utilization which is directed to the source part which is a cognitive variable. In addition, the research also shows that there is no significant effect of the cognitive variables toward attitude variable, comparing to the effect toward purchase intention for the case of sensual appeals advertisement in woman's point of view.

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[ABSTRAK
Latar Belakang: Kebocoran mikro dinding gingiva restorasi resin komposit proksimal sering terjadi. Penelitian ini bertujuan untuk menganalisis perbedaan tingkat kebocoran mikro dinding gingiva antara RK flowable konvensional dan modifikasi dengan teknik precured dan cocured. Metode: 120 kavitas kelas II Black gigi premolar RA dibagi menjadi empat kelompok. Setelah dipreparasi berbentuk boks, kelompok 1 direstorasi dengan teknik precured (liner konvensional dan modifikasi) , kelompok 2 dengan teknik cocured (liner konvensional dan modifikasi), kelompok 3 dengan liner RK flowable konvensional (teknik precured & cocured),dan kelompok 4 dengan liner RK flowable modifikasi (teknik precured & cocured). Pengukuran penetrasi zat warna biru metilen 1% dilakukan setelah thermocycling. Gigi kemudian dibelah mesiodistal dan diamati menggunakan mikroskop stereo 2 pembesaran 25x. Analisis data dengan uji Kolmogorov-Smirnov. Hasil: Terdapat perbedaan bermakna antarkelompok teknik satu dan dua dengan tingkat kebocoran teknik cocured lebih rendah (p= 0.047) dan tidak terdapat perbedaan bermakna antara kelompok tiga dan empat (p= 0.985). Kesimpulan: Teknik cocured memiliki tingkat kebocoran lebih rendah dibandingkan dengan teknik precured. Namun tidak terdapat perbedaan bermakna antara kelompok dengan liner RK flowable konvensional dan modifikasi.

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ABSTRACT
Background: Microleakage of composite restoration in proximal often occurs on gingival wall. The purpose of this study to analyze the microleakage of gingival wall in composite restoration using technique cocured and precured with conventional and modification liner. Methods: Standardized class II were prepared on 60 extracted human upper premolar mesial and distal into 4 groups. Within a box-like cavities, the first group is restored with precured technique (conventional & modification liner), second group is restored with cocured technique (conventional & modification liner), third group is restored with conventional liner (precured & cocured technique), and fourth group is restored with modification liner (precured & cocured technique). The specimens were subjected to thermocycling, followed by immersion in 1% methylene blue dye for 24 hours. The teeth were sectioned mesio-distally and evaluated for microleakage under 25x magnification steremicroscope and score in ordinal scale (0-3). Statistical analysis was performed with the Kolmogorov-Smirnov test. Results: There was significant difference between first and second group, which is microleakage in second group lower than first (p= 0.047). There was no significant difference between third group and fourth (p= 0.985). Conclusion: Cocured technique have lower microleakage than precured technique. But there is no significance difference between conventional liner group and modification liner. ;Background: Microleakage of composite restoration in proximal often occurs on gingival wall. The purpose of this study to analyze the microleakage of gingival wall in composite restoration using technique cocured and precured with conventional and modification liner. Methods: Standardized class II were prepared on 60 extracted human upper premolar mesial and distal into 4 groups. Within a box-like cavities, the first group is restored with precured technique (conventional & modification liner), second group is restored with cocured technique (conventional & modification liner), third group is restored with conventional liner (precured & cocured technique), and fourth group is restored with modification liner (precured & cocured technique). The specimens were subjected to thermocycling, followed by immersion in 1% methylene blue dye for 24 hours. The teeth were sectioned mesio-distally and evaluated for microleakage under 25x magnification steremicroscope and score in ordinal scale (0-3). Statistical analysis was performed with the Kolmogorov-Smirnov test. Results: There was significant difference between first and second group, which is microleakage in second group lower than first (p= 0.047). There was no significant difference between third group and fourth (p= 0.985). Conclusion: Cocured technique have lower microleakage than precured technique. But there is no significance difference between conventional liner group and modification liner. , Background: Microleakage of composite restoration in proximal often occurs on gingival wall. The purpose of this study to analyze the microleakage of gingival wall in composite restoration using technique cocured and precured with conventional and modification liner. Methods: Standardized class II were prepared on 60 extracted human upper premolar mesial and distal into 4 groups. Within a box-like cavities, the first group is restored with precured technique (conventional & modification liner), second group is restored with cocured technique (conventional & modification liner), third group is restored with conventional liner (precured & cocured technique), and fourth group is restored with modification liner (precured & cocured technique). The specimens were subjected to thermocycling, followed by immersion in 1% methylene blue dye for 24 hours. The teeth were sectioned mesio-distally and evaluated for microleakage under 25x magnification steremicroscope and score in ordinal scale (0-3). Statistical analysis was performed with the Kolmogorov-Smirnov test. Results: There was significant difference between first and second group, which is microleakage in second group lower than first (p= 0.047). There was no significant difference between third group and fourth (p= 0.985). Conclusion: Cocured technique have lower microleakage than precured technique. But there is no significance difference between conventional liner group and modification liner. ]

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Latar belakang: Penglepasan ion kalsium oleh material bioaktif dapat berperan penting dalam peningkatan pH yang diperlukan dalam aktivitas antibakteri dan remineralisasi jaringan keras gigi. Tujuan: untuk menganalisis pelepasan ion kalsium dan peningkatan pH dari MTA modifikasi dan Bioceramic pada periode waktu 1,48,168 jam. Metode: Sampel n=30 dipersiapkan dengan ukuran diameter 3 mm tinggi 3 mm, terdiri dari 15 sampel MTA modifikasi, 15 sampel Bioceramic direndam dalam air deionisasi 1,48,168 jam diukur kadar pelepasan ion kalsium menggunakan AAS dan nilai pH menggunakan pHmeter, Uji Kruskal Wallis dan Mann Whitney. Hasil: Terdapat perbedaan yang bermakna diantara semua kelompok dengan nilai signifikansi p le;0,05. Kesimpulan: Bioceramic terbukti melepaskan ion kalsium dan peningkatan pH lebih besar dibandingkan dengan MTA modifikasi pada waktu pengukuran 1,48,168 ......Background: Calcium ion release can promote alkalinizing activity and regeneration. Objective: To analyze calcium ion release and pH changes from modified MTA and Bioceramics as bioactive material. Methods: 30 samples are prepared with the size of 3 mm in diameter and 3 mm in height. The samples are consist of 15 of modified MTA and 15 of bioceramics. And then immersed in deionized water for an hour which will then be measured in 1, 48, and 168 hours period. And measured atom absorption sphectropometer and pHmeter. Result: Mann Whitney post hoc rsquo s statistic test result showed a significant discrepancy among all groups, with the significant value of p le 0,05. Conclusion: Bioceramics was proven to release more calcium ions and more pH elevation compared to modified MTA during the 1 hour, 48 hour, and 168 hours measurements.

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ABSTRAK
Latar Belakang: E. faecalis merupakan bakteri yang sulit dieliminasi sehingga dapat menyebabkan kegagalan perawatan endodontik. Klorheksidin 2 merupakan bahan irigasi yang sudah terbukti efektif dalam mengeliminasi E. faecalis, namun memiliki toksisitas terhadap sel-sel yang sehat. Ekstrak jintan putih Cuminum cyminum memiliki potensi efektivitas antibakteri. Namun, belum terdapat penelitian yang meneliti efek antibakteri ekstrak jintan putih terhadap biofilm E. faecalis dari isolat klinis. Tujuan: Mengetahui efek antibakteri ekstrak jintan putih konsentrasi 0,2 mg/ml, 0,5 mg/ml, 0,7 mg/ml, 1,0 mg/ml, dan 1,2 mg/ml dibandingkan dengan klorheksidin 2 terhadap biofilm E.faecalis dari isolat klinis. Metode: Menilai kekeruhan larutan biofilm E. faecalis pasca pemaparan bahan uji dengan ELISA reader, dengan hasil akhir berupa nilai optical density OD . Hasil: Terdapat perbedaan efek antibakteri yang bermakna antara ekstrak jintan putih dengan klorheksidin 2 terhadap biofilm E.faecalis dari isolat klinis p < 0,05 . Kesimpulan: Efek antibakteri ekstrak jintan putih konsentrasi 1,0 mg/ml lebih baik dibandingkan dengan klorheksidin 2 terhadap biofilm E.faecalis dari isolat klinis.

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ABSTRACT
Introduction E. faecalis is a bacteria that is difficult to eliminate which can lead to failure of endodontic treatment. Chlorhexidine 2 is an endodontic irrigation material that has been proven to be effective against E. faecalis, but has toxicity to healthy cells. The extract of cumin Cuminum cyminum has the potential antibacterial activity. However, there have been no research investigating the antibacterial effect of Cuminum cyminum extract on E. faecalis biofilm from clinical isolates. Aims To compare antibacterial efficacy of Cuminum cyminum extract 0,2 mg ml, 0,5 mg ml, 0,7 mg ml, 1,0 mg ml, and 1,2 mg ml and 2 chlorhexidine against E. faecalis biofilm from clinical isolates. Methods Assessing the turbidity of E. faecalis in biofilm after immersed in antibacterial agents with ELISA reader, with optical density OD as the final result. Results There were significant differences statistically between Cuminum cyminum extract and 2 chlorhexidine against E. faecalis biofilm from clinical isolates p 0.05 . Conclusion Antibacterial effect of 1,0 mg ml Cuminum cyminum extract was more effective than 2 chorhexidine against E. faecalis biofilm from clinical isolates.

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Latar Belakang: Affected dentin merupakan lapisan yang masih dapat terjadi remineralisasi karena masih terdapat ikatan silang kolagen dan prosesus odontoblastik yang masih vital yang merupakan syarat terjadinya remineralisasi. Terdapat dua metode remineralisasi, yaitu metode konvensional dan guided tissue remineralization GTR. Pada metode konvensional hanya dapat terjadi remineralisasi secara ekstrafibrillar. Sedangkan pada metode GTR memiliki keunggulan yaitu dapat terjadi remineralisasi secara ekstrafibrillar dan intrafibrillar. Beberapa penelitian melaporkan bahwa mineralisasi intrafibrillar dapat meningkatkan properti mekanis dari dentin. Pada metode GTR dibutuhkan peran protein non kolagen, yaitu DMP 1. Namun karena proses karies, maka sebagian DMP 1 mengalami kerusakan sehingga dibutuhkan material analog protein non-kolagen, salah satunya adalah Carboxymethyl Chitosan/Amorphous Calcium Phosphate CMC/ACP. Tujuan: Mengevaluasi terjadinya remineralisasi intrafibrillar pada permukaan demineralized dentin setelah aplikasi material analog protein non-kolagen CMC/ACP. Metode: Empat kelompok dilakukan demineralisasi buatan, dalam satu gigi terdapat dua kavitas, salah satu kavitas diaplikasikan material CMC/ACP, sedangkan kavitas lainnya tidak diaplikasikan CMC/ACP. Sampel diperiksa pada hari ke-7 dan ke-14 dengan Transmission Electron Microscope TEM. Hasil: Terlihat peningkatan kadar kalsium dan fosfat setelah aplikasi CMC/ACP pada hari ke-7 dan ke-14. Kesimpulan: CMC/ACP memiliki potensi untuk meremineralisasi demineralized dentin. ......Affected dentin is a layer which can be remineralized due to the presence of cross linked collagen and a living odontoblastic process a key to remineralization. There are two methods of remineralization convensional and guided tissue remineralization GTR. In conventional methods, only extrafibrillar remineralization occurs. GTR resulting intrafibrillar and extrafibrillar remineralization. Intrafibrillar remineralization improves physical properties of dentin. GTR is a method of collagen dentin remineralization using non collagen protein, Dentin Matrix Protein 1 DMP 1. DMP 1 is damaged due to caries process. Carboxymethyl Chitosan Amorphous Calcium Phosphate CMC ACP has similar function with DMP 1. Aim: To evaluate intrafibrillar remineralization on demineralized dentin after application non collagen protein analog CMC ACP. Method: Four groups performed artificial demineralization two of which applied CMC ACP material. Whereas, the other group was not applied CMC ACP. Evaluation of intrafibrillar remineralization with Transmission electron Microscope TEM. Result: After 7 days and 14 days CMC ACP application, intrafibrillar remineralization was observed in the gap zone. Conclusion CMC ACP has a potential for intrafibrillar remineralization on demineralized dentin.

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Latar Belakang : Penyebab kegagalan perawatan saluran akar adalah kebocoran apeks yang ditentukan oleh teknik pengisian saluran akar. Tujuan : Untuk menganalisis kebocoran sepertiga apeks pada pengisian saluran akar menggunakan GuttaCore™. Metode : Dua puluh delapan gigi premolar bawah yang telah dilakukan pengisian saluran akar, diinkubasi (37°C, 24 jam), kemudian direndam dalam tinta india selama 7X24 jam. Sampel didekalsifikasi sampai transparan. Kedalaman penetrasi tinta dievaluasi dengan mikroskop stereo. Hasil : Seluruh pengisian dengan GuttaCore™ terlihat adanya penetrasi tinta sedalam 0-0.5 mm. Kesimpulan : Hasil pengisian saluran akar dengan teknik GuttaCore™ dapat menurunkan tingkat kebocoran di sepertiga apeks. ......Background : The cause of endodontic treatment failure is apical leakage determined by root obturation technique. Aim:To analyze leakage in apical third with GuttaCore™ obturation. Method : Twenty eight lower premolars were obturated and incubated (37*C, 24 hours) then immersed in indian ink for 7x24 hours. Samples were decalcified until transparent. Depth of ink penetration was evaluated using stereo microscope. Result: All obturation using GuttaCore™ technique resulted in 0-0.5 mm ink penetration Conclusion : Root obturation with GuttaCore technique is able to decrease leakage in apical third