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Oentoeng Iskandar
"ABSTRAK
Sel limfosit darah perifer dan sel fibroblast kulit manusia, yang telah dibenihkan dapat dipakai untuk penelitian mutagenita in vitro. Begitu pula SCE dapat digunakan sebagai titik akhir biologik untuk mengukur mutagenitas beberapa agens. Pambenihan sel limfosit darah perifer mudah dan sederhana. Oleh karena itu sel limfosit merupakan bahan pilihan terbaik untuk menganalisa kromosom dalam pemeriksaan rutin diagnostik sitogenetik. Pada keadaan-keadaan tertentu, misalnya pada pemeriksaan ?point mutation" pembenihan sel fibroblast sangat diperlukan. Penelitian-penelitian yang dikemukakan dalam tesis ini diawali dengan usaha untuk mengembangkan suatu metoda baru yang lebih baik, untuk mendeteksi micronucleus didalam sel darah limfosit perifer, sehingga dapat digunakan untuk mengukur efek-efek mutagen. Karena micronucleus itu berasal dari fragmen kromosom yang tidak mengandung sentromir, sehingga ditinggalkan di dalam sitoplasma ?daughter cell" pada mitosis, maka adalah penting pada metoda
yang diperbaiki itu, untuk mempertahankan keutuhan sitoplasma sel limfosit. Pencampuran sel darah limfosit perifer dengan suatu larutan hipotonik lemah, merupakan suatu langkah penting untuk mempertahankan agar sitoplasma itu tetap utuh. Secara eksperimental dapat ditemukan bahwa larutan hipotonik lemah tersebut ialah suatu campuran garam faali (0,9 8 natriu klorida) dan 5,6 gr/1 kalium klorida dengan perbandingan 10 dan 1. Campuran ini ternyata mempunyai tekanan osmotik sebesar 285 milliosmol. Bila metoda ini digunakan, maka membedakan suatu
struktur yang mirip micronucleus (umpama fragmen dari sel yang sedang mengalami kemunduran dan akan mati) dan micronucleusnya sendiri tidaklah sukar lagi. Ternyata metoda yang telah diperbaiki ini dapat pula diterapkan pada sel fibroblast manusia, untuk mempertahankan agar sitoplasma itu utuh, sehingga micronuoleus yang terletak didalamnya dapat dideteksi. (lihat karangan IV dan gambar 16). Sel fibroblast itu berubah menjadi hulat, dengan sitoplasma yang utuh. Bila didalamnya terdapat micronucleus, akan tampak jelas. Frekuensi micronucleus spontan pada penderita "chromosomal breakage disease" (Fanconi'S anemia, karangan IV; Blackfan Diamond, karangan II; Down?s syndrome, karangan VI) dengan mudah dapat ditentukan. Ternyata freknensi spontan pada penderita penyakit tersebut lebih tinggi daripada pada orang normal. Karena micronucleus ltu didalam sitoplasma dapat tinggal lama, maka sel yang mengandunqnya dapat diperiksa dari pembenihan, pada saat mana saja setelah proses tersebut dimulai. (Yang optimum adalah 72 jam setelah dibenihkan). Dari panelitian dapat disimpulkan bahwa frekuensi micronucleus spontan dapat digunakan untuk mandeteksi adanya suatu "chromosome breakage syndrome". Kesederbanaan atau mudahnya tehnik yang diperbaiki ini sehagai metoda untuk pemeriksaan atau pengukuran efek-efek mutagen dapat dilihat dari makalah II dan VI untuk MMC dan sinar X, makalah III untuk Aflatoxin B1, serta karangan IV untuk acataldehida dan akhirnya karangan V untuk EMS dan MMC. Peningkatan efek-efek mutagen Aflatoxin B1 olah ?S9 mix" dapat diukur dengan frekuensi MN yang meningkat, hal mana dapat dipelajari di makalah III. Analisa kelainan atruktur kromosom dan
frékuensi MN (yang timbul karena dirangsang) pada yenderita Down's syndrome dan pada orang normal disajikan dalam tulisan VI. Didalam eksperimen ini ditunjukkan pula kepekaan sel limfosit darah penderita Down's syndrome pada sinar X. yang mennnjukkan adanya hubungan dengan umur, serta refleksinya dapat terlihat pada frekuensi disentrik dan M yang meninggi. Data yang diperoleh, memperkhat pula kesimpulan-kesimpulan Hedrile yang menyatakan bahwa meningkatnya frekuensi MN lebih merupakan refleksi kelainan jenis "exchanges" daripada frekuensi " chromosome breaks ". Suatu kesan yang kuat tentang kemungkinan adanya suatu respons in vitro yang berbeda dan bersangkut-paut dengan sex, disajikan dalam karangan VI. Tldak adanya perbedaan respons semacam itu untuk MMC, juga disajikan dalam karangan tersebut. Pada penelitian ini meningkatnya frekuensi M yang berbeda antara pria dan wanita akibat exposure pada EMS, merupakan hukti adanya perbedaan yang herkaitan dengan sex. Kesimpulan ini diperkuat dengan analisa dari frekuensi SCE yang meninggi pada wanita, akibat pemaparan sel limfosit A nya pada BMS. Frekuensi MN akibat pemaparan pada EMS, nenunjukkan perbedaan yang menyolok antara pria dan wanita, tetapi tidaklah demikian halnya dengan response terhadap MMC. Karena data SCE tidak dapat dihasilkan untuk donor VI, suatn kesimpulan yang tegas belumlah dapat dibuat. Tetapi penemuan lain, menimbulkan dugaan yang kuat tentang adanya suatu respons yang
herbeda terhadap EMS bagi pria dan wanita, tetapi tidak untuk MMC. Hasil-hasil yang didapat dari semua eksperimen menunjukkan bahwa metoda yang diperbaiki untuk mendeteksi MN dalam sel limfosit manusia dan sel fibroblast itu mudah, cepat dan dapaf disesuaikan dengan tujuan suatu penelitian. Karenanya faedah dari etoda ini dihari kamudian nampaknya besar. Jadi apa yang diramalkan dalam hipotesa itu ternyata benar.

ABSTRACT
Peripheral blood lymphocytes and cultured skin fibroblasts can be used as materials for in vitro mutagenicity studies, using chromosomal aberrations, micronuclei, as well as SCEs as biological endpoints for assessing mutagenicity of diverse agents. However, for routine diagnostic cytogenetic work, in clinics culture of peripheral blood lymphocytes is really the material of choice, in view of the ease and simplicity with which they can be cultured and processed for chromosomal analysis. In some situations, such as point mutation studies, culture of fibroblasts is essential. The studies in this thesis were initiated to develop an improved method for the detection of MN in peripheral human lymphocytes, which can be used for assaying effects of mutagens. As a micronucleus is left behind in the cytoplasm of a daughter cell following cell division, it should be important in an improved method to preserve the cytoplasm of the lymphocytes intact. Mild hypotonic treatment was found to be the important step in preserving cytoplasm. This mild hypotonic solution consists of a mixture of physiological saline (0.9% sodium chloride) and 5,6 gr/l potassium chloride with a proportion of 10 to 1, of which the osmotic pressure was found to be 285 milliosmol.
To distinguish an intracytoplasmic localized micronucleus and other structures resembling M is very easy by this method. This improved method seems also to be applicable for human fibroblasts, with regard to preserving the cytoplasm intact for detecting a micronucleus (see paper IV and figure 16), The fibroblasts are transformed to a spherical shaped cell with an intact cytoplasm and a micronucleus inside when present. The frequencies of spontaneous M in normal individuals, and in patients with chromosomal breakage disease (Fanconi's anemia, paper IV; Blackfan Diamond, paper II; and Down's syndrome paper VII), can be easily determined. The frequencies are higher in chromosomal breakage syndrome when compared to normal. Since MN persists for a long time, a larger number of cells carrying them can be scored in the culture at any time following initiation. From these studies we can conclude that the frequencies of spontaneous MN can be used easily for detection of a chromosome breakage syndrome. The ease with which the improved method can be used as a method of assay for effects of mutagens are also demonstrated in papers II, VI, VII for MMC and X-ray irradiation, paper III for Aflatoxin Bl, paper IV for acetaldehyde, paper VI for EMS and MMC.
The enhancement of effect of Aflatoxin B1 by S9 mix, assessed by the frequencies of induced MN, is.also shown in paper III.
An analysis of the induced chromosomal aberrations and micronuclei in Down's syndrome patients and in normal individuals is presented in paper VII. In this experiment is shown the age related sensitivity the patients to X-ray, which is reflected in the yield of both dicentrics and micronuclei: Our data also support Heddle's conclusion that the increase of frequencies of micronuclei is more a reflection of the exchange type of aberrations, than the frequencies of chromosome breaks.
A strong.suggestion about the possible existence of a sex related difference of in vitro responses between male and female to aus is presented in paper VI. The absence of such difference for MMC is also shown in that paper. In this study, the differential increase of frequencies of induced M , following exposure to EMS between male and female, provides some evidence to conclude a sex related difference of responses. This conclusion is supported by the analysis of the frequencies of induced SCEs (for each treatment as well as controls) The frequencies of induced HN, show a considerable difference between male and female donors, which is not the case following exposure to MMC. As SCE data are not available for donor VI, a firm conclusion cannot be reached as yet. But the other findings, strongly suggest the existence of a sex related response to EMS, but not for MMC.
The results obtained from all experiments indicate that the improved method for the detection of MN in human lymphocytes and fibroblasts, is easy, fast amdadaptable to the aim of an investigation. Therefore the utility of this method in the future investigations appears to be high, It is clear, that what is stated in the hypothesis is true."
1981
D1102
UI - Disertasi Membership  Universitas Indonesia Library
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Much Taufik Tri Hermawan
"Research on usable of Gunung Gede Pangrango National Park and Botanical Garden to develop the conservation education has been carried out, but the available information due to the package of conservation education using both of conservation areas is stilt lacking.
The aim of this study is to measure and compare conservation education packages, which have conducted at Gunung Gede Pangrango and Bogor Botanical Garden. The compiling information from this study will be useful as a contribution to develop the program of conservation education as well as to increase the management of conservation area.
The comparative study was conducted in two conservation area, the Gunung Gede Pangrango (representative for in situ conservation), and Bogor Botanical Garden (for ex situ conservation), using a questionnaire method, document analyzing, field observation, and measure the progress of the institutional development using matrix of the Institutional Development Framework ( IDF ). Correspondence is the key-person from institutions who responsible in organizing the conservation education program.
The conclusion of this study was that the Gunung Gede Pangrango National Park is more diverse in having potential interpretation material compare to that of Bogor Botanical Garden, especially the available concepts of ecology and conservation in species and ecosystem level. In comparison, the Bogor. Botanical Garden has relatively more facilities on species interpretation. The conservation education program at both areas has limit goal, only covered the awareness and knowledge, and not completed with the education evaluation, which measured the impact of education on conservation for the participants. Covering the goal of the education, usable of flagship species and education evaluation might develop the quality of the program at both areas.
Both of the institutions have limit facilities and skill to develop the package of the conservation education program, therefore they need cooperation with other institution as a partner. The study also identified that the Partners have a good contribution on developing the package of the Conservation education program as well as provide human resources. Some partners have provided the technical and facilities for educating, however none of the four NGO's which cooperate with the two institutions (Gunung Gede Pangrango National Park and Bogor Botanical Garden) in sustainability stage at institutional; development continuum. It seem that their institutional development progress will influence the existing packages for educating conservation program, which prepared at Gunung Gede Pangrango National Park and Bogor Botanical Garden."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 1999
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UI - Tesis Membership  Universitas Indonesia Library
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Utari Budihardjo
"Hasil penelitian tentang di Madura yang dilakukan pada bulan Juli 1995 sampai dengan bulan Juli 1996 telah diketemukan empat species Baru di perairan Indonesia, yaitu Solen abbreviatus, Solen comeus, Solen malaccensis dan Solen timorensis. Solen merupakan makanan tambahan untuk lauk pauk, cemilan dan sebagai penyedap (petis). Solen regularis, Solen malaccensis dan Solen leanus mempunyai kandungan protein masing-masing 10,73%;12,34%; 11,29%. Kandungan karbohidrat 4,1%, 5,39% dan 6,66%. Sedangkan kandungan lemak 1,22% dan 1,29 % dan kandungan air sebesar 79,33%, 79,46% dan 75,68%."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 1996
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UI - Tesis Membership  Universitas Indonesia Library
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Supardiyono
"Five Dayak ethnic groups : Iban, Tamambaloh, Kantu', Bukat and Punan are residing inside the Bentuang Karimun National Park and its vicinity. Most Dayak members are earning their life as farmers, hunters, poachers and collective of forest products either for commercial or personal purposes. They live harmoniously with their surrounding.
Research on "Biodiversity of plants and the utility of landscape by communities of Dayak ethnics at Bentuang Karimun National Park and its vicinity", were carried out from November 1996 to May I997. The result had been presented in a thesis, which is composing of two subjects.
First subject, concerning with "The indigenous knowledge and the landscape usage of the Dayak". This study is separated into two consecutive chapters.
Data were collected by using exploration and square methods. Diversity index are obtained by using Shanon & Wiener formula 1949 (Kreb, 1989); Equation of plant community are calculated using Jaccard (Greig-Smith, 1983) and Cluster analysis is performanced by Ludwig & Reynolds formulation (1988).
The result of the research shows that each ethnic group has particular characteristic as follows :
a. Housing
Sadap village (Dayak Iban), and Sungai Ulu' Palin ViIIage (Dayak Tamambaloh) have long and single house. The other villages such as Nanga Potan (Dayak Kantu'), Along Hovat (Dayak Bukat) and Nanga Bungan (Dayak Punan) do not have. Sadap village is the only village that has water pipe system, while other four villages do not. They get water from the river and from the rain water for drinking. At Along Hovat and Nanga Bungan village, the road has been made from concrete base, while the other three villages have not. Sungai Ulu' Palin village of the Dayak ethnic has the highest population, which. consisted of 92 families or 403 persons, while Nanga Potan village of the Dayak has the lowest, which consisted only 10 families or 45 persons.
b. Home garden
Based on the plant composition of the home garden at the five villages we visited that the Dayak practice different type of home garden . At Sadap village and Sungai Ulu' Palin village : the Iban and Tamambaloh prefer to grow industrial plants such as rubber Ffevea brasilliensis), while at Nanga Potan and along Hovat : Kantu' and Bukat prefer to grow fruits plants, such as jackfruit (Artocarpus heterophyllus) and durian (Dario zibethinus). However, the Punan at Nanga Bungan does not practice home garden , therefore, there is no any special plants in their garden.
c. Variaton of plants at the active field
Generally, at the five villages there are about 27 varieties of glutinous rice and 77 varieties of rice. Beside the main plant, they also grew the supplementary plants such as cucumber (Cucumis melo), cassava (Manihot esculenta), egg plant (Soiwnan sp.), small chili (Capsicum frutescens), "katuk" (Sauropus albicans), ginger (Zingiber officinale) and "paria" (Momordica charantia).
d. Vegetation of the abandoned field.
The composition of the plants at the abandoned garden has variation. The abandoned garden for 1 - 5 years were dominated by wild plants such as Themeda gigantea and Melastoma malabathricum. The abandoned garden for 5 - 10 years, were dominated by secondary plants such as Macaranga gigantea, A. trilaba, M lapadanta, and Threma orientalis. The abandoned field of more than 20 years are primarily dominated by Dipterocarpaceae.
e. Cluster analysis
Based on what we observed , we categorize three stages of growth such as "belta" , "trees", and "seedling" stadium on the field. There is an indication that the factor of human activities influence the plant's growth.
Second subject, "Me indigenous knowledge and the utility of plant diversity of Dayak ethnic at the Bentuang Km-isms National Park and its vicinity". The purpose of the research was to study how the communities around the National Park benefing the landscape of the park. The data obtained by interviewing members of the community.
a. The using of plants
Categorization of plant use in five villages communities :
1. Edible plants : consisted of 129 species, 94 genus, and 42 families.
2. Housing materials : consisted of 63 species. 21 genus_ and 14 families.. And tfor agricultural tool consisted of 23 species, 13 genus, and 9 families.
3. Medicinal purposes : consisted of 55 species. 51 genus. and 34 families,
4. Ceremonials and rituals : consisted of 21 species, 19 genus, and 17 families.
5. Clothing : consisted of 7 species, 6 genus, and 5 families.
6. Utensils,rope and braid consisted of 27 species. 14 genus, and 9 families.
7. Dyes : consisted of 7 species, 7 genus, and 6 families.
8. Firewood consisted of 19 species, 11 genus and 9 families
9. Ornamental or decoration : consisted of 7 species, 6 genus, and 5 families.
10. Poison and toxic ingredient : consisted of 4 species, 3 genus, and 2 families.
b. The indigenous knowledge
The indigenous knowledge of plants of the communities among the ethnics shows varies from one to another. The differences in the knowledge between those of the male and those of the female were tested using the proportional test."
Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 1999
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UI - Tesis Membership  Universitas Indonesia Library