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"Sayur asin umumnya dikenal sebagai produk fermentasi spontan sawi pahit, Brassica juncea (L.) Czern., oleh bakteri asam laktat (BAL) epifit di Indonesia, khususnya di Jawa. Informasi keanekaragaman BAL di sayur asin lokal Indonesia masih sangat terbatas, oleh karena itu perlu dilakukan penelitian lebih lanjut. Penelitian dilakukan untuk mengetahui keanekaragaman BAL di sayur asin lokal Indonesia. Telah dilakukan isolasi dan identifikasi BAL dari sebelas sampel sayur asin diperoleh dari Tulung Agung, Kediri, Solo,Yogyakarta, Kota Semarang dan Depok. BAL diisolasi dari sayur asin dan larutan fermentasi. Identifikasi BAL dilakukan secara molekuler berdasarkan sekuen 16S rDNA. Sebanyak 631 isolat BAL telah berhasil diisolasi dan diidentifikasi. Hasil identifikasi menunjukkan 20 spesies BAL yang telah diketahui namanya yaitu Lactobacilus farciminis (90 isolat), L. fermentum (106 isolat), L. namurensis (48 isolat), L. plantarum (309 isolat), L. paralimentarius (4 isolat), L. parafarraginis (1 isolat), L. nodensis (1 isolat), L. tucceti (11 isolat), L. acidophilus (1 isolat), L. helveticus (2 isolat), L. brevis (4 isolat), L. parabrevis (3 isolat), L. futsaii (11 isolat), L. versmoldensis (4 isolat), L. coryniformis (16 isolat), L. casei (12 isolat), L. rhamnosus (2 isolat), L. fabifermentans (3 isolat), L. satsumensis (1 isolat), L. zymae (1 isolat) dan 1 kandidat spesies baru Lactobacillus sp. B4 (1 isolat) secara genetik dekat dengan L. composti. Hasil identifikasi menunjukkan keanekaragaman BAL di sayur asin asal Jawa Indonesia cukup tinggi. Analisis intraspesies pada spesies dominan L. plantarum dan L. fermentum asal sayur asin menggunakan tiga teknik molekuler restriction fragment length polymorphism (RFLP) 16S-23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) dan enterobacterial repetitive intergenic consensus (ERIC-PCR) menunjukkan terdapat tiga kelompok genotip L. plantarum dan dua kelompok genotip L. fermentum asal sayur asin.

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Sayur asin is commonly known as a spontaneous fermented mustard Brassica juncea (L.) Czern product by epiphytic lactic acid bacteria (LAB) in Indonesia, in particular Java. The information on LAB diversity in Indonesia was obviously limited. More investigation is required. Therefore, effort on inventory of LAB from sayur asin in Indonesia was carried out to determine its diversity. LAB diversity in eleven samples of sayur asin collected from Tulung Agung, Kediri, Solo, Yogyakarta, Kota Semarang and Depok was studied. LAB was isolated from both of fermented mustards and fermenting liquors. Molecular identification of the isolates was conducted based on 16S rDNA sequence data. A total of 631 isolates of LAB was successfully isolated and identified. The bacteria belong to 20 known species viz, Lactobacilus farciminis (90 isolates), L. fermentum (106 isolates), L. namurensis (48 isolates), L. plantarum (309 isolates), L. paralimentarius (4 isolates), L. parafarraginis (1 isolate), L. nodensis (1 isolate), L. tucceti (11 isolates), L. acidophilus (1 isolate), L. helveticus (2 isolates), L. brevis (4 isolates), L. parabrevis (3 isolates), L. futsaii (11 isolates), L. versmoldensis (4 isolates), L. coryniformis (16 isolates), L. casei (12 isolates), L. rhamnosus (2 isolates), L. fabifermentans (3 isolates), L. satsumensis (1 isolate), L. zymae (1 isolate) and 1 candidate of novel species Lactobacillus sp. B4 (1 isolate) phylogenetically closed to L. composti. The current study revealed high diversity of LAB present in sayur asin from Java, Indonesia. Study on intraspecies determination of predominant isolates belonging to L. plantarum and L. fermentum in sayur asin using three molecular techniques namely: restriction fragment length polymorphism (RFLP) 16S-23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and enterobacterial repetitive intergenic consensus (ERIC-PCR) revealed that strains belong to L. plantarum and L. fermentum in sayur asin could be divided into three and two genotypic groups respectively"

"Penelitian telah dilakukan mengenai karakteristik Eichhornia crassipes (Mart.) Solms dan Pistia stratiotes L. pada air limbah domestik serta uji toksisitas hasil fitoremediasinya. Penelitian bertujuan untuk mengetahui aspek anatomi, fisiologi dan ekologi E. crassipes dan P. stratiotes serta toksisitas hasil fitoremediasinya terhadap Dahnia magna L. dan Cyprinus carpio L. Bahan ditempatkan di outlet kolam anaerob, fakultatif dan maturasi di Instalasi Pengolahan Air Limbah (IPAL) Bojongsoang Bandung selama 14 hari dan sebagai fitoremediator selama 10 hari dengan metode statik. Hasil fitoremediasi diuji toksisitas akut dan sub akut terhadap D. magna selama 2 hari dan C. carpio selama 4 hari. Hasil penelitian menunjukkan E. crassipes dan P. stratiotes sebagian besar menurun pada diameter akar 1,18-2,50 mm, stele 0,37-1,82 mm, korteks 0,30-0,65 mm, panjang akar 0,05-5,21 cm dan kadar klorofil 0,19-1250,33 mg/L, serta meningkat pada berat basah 93,39-99,49 g, panjang stolon 11,33-15,97 cm, panjang petiola 2,05-3,21 cm dan luas daun 1,12-8,56 cm2; kelimpahan bakteri nitrifikasi pengoksidasi amonia (AOB) dan bakteri pengoksidasi nitrat (NOB) meningkat pada rhizosfer E. crassipes. Efisiensi tertinggi E. crassipes 86,14% fosfat dan 98,41% nitrat dengan retensi terendah 0,3-0,4 hari, serta tertinggi P. stratiotes 96,34% TSS, 97,20% kekeruhan dan 96,70% BOD. Kadar nitrat di akar lebih tinggi dibanding pada daun. Hasil fitoremediasi menunjukkan toksik rendah, meningkatkan rata-rata telur 12,1-14,7, frekuensi bertelur 0,7 dan awal hari bertelur 3,5-3,6 hari, serta peningkatan laju konsumsi oksigen 150,8-239,1 mg/g bb/jam C. carpio pada hasil fitoremediasi E. crassipes. Hasil-hasil tertinggi sebagian besar diperoleh pada air limbah domestik dari kolam anaerob dan fakultatif. Eichhornia crassipes dan Pistia stratiotes adaptif pada air limbah domestik, berpotensi tinggi dalam menurunkan polutan air limbah domestik dengan hasil fitoremediasinya toksik rendah.

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Research has been done on the characteristics of Eichhornia crassipes (Mart.) Solms and Pistia stratiotes L. in domestic wastewater and phytoremediation results toxicity test. The study aims to determine anatomy, physiology and ecology aspect, and the toxicity test of phytoremediation results against Daphnia magna L. and Cyprinus carpio L. Materials placed on the outlet in anaerobic, facultative and maturation ponds on Wastewater Treatment Plant (WWTP) Bojongsoang Bandung for 14 days, and as phytoremediator for 10 days using static methods. Acute toxicity tests and sub acute phytoremediation results for D. magna reproduction for 2 days and the rate of oxygen consumption C. carpio for 4 days. The results showed in E. crassipes and P. stratiotes most of the decrease in root diameter 1.18-2.50 mm, stele 0.37-1.82 mm, cortex 0.30-0.65 mm, root length 0.05-5.21 cm and chlorophyll content 0.19-1250.33 mg/L, and the increase in wet weight 93.39-99.49 g, stolon length 11.33-15.97 cm, petiole length 2.05-3.21 cm and leaf area 1.12-8.56 cm2; abundance of nitrifying ammonia oxidizing bacteria (AOB) and nitrate oxidizing bacteria (NOB) is increased in the rhizosphere of E. crassipes. The highest efficiency of E. crassipes 86.14% phosphate and 98.41% nitrate, and P. stratiotes 96.34% TSS, 97.20% turbidity and 96.70% BOD, and the lowest retention of 0.3-0.4 days. Nitrate levels in roots was higher than in the leaves. Domestic wastewater toxicity test phytoremediation results to D. magna and C. carpio showed low toxic. Subacute toxicity tests on D. magna reproduction showed increase the average egg 2.1-14.7, the frequency spawn about 0.7 and earlier in the day spawn 3.5-3.6 days, and increase in the rate of oxygen consumption 150.8-239.1 mg/g w/h C. carpio on E. crassipes phytoremediation results. The results mostly takes place in the wastewater from the anaerob and facultative ponds. Eichhornia crassipes and Pistia stratiotes adaptive in domestic wastewater, high potential in reducing."

"Penelitian bertujuan untuk memperoleh dan mengidentifikasi isolat-isolat bakteri endofit yang potensial senyawa bioaktif antidiare dari tanaman N. altissima; mendeteksi, memurnikan dan mengidentifikasi senyawa bioaktif antidiare yang dihasilkan; serta menganalisis mekanisme kerjanya dalam menghambat pertumbuhan bakteri uji. Bakteri endofit diisolasi dari bagian akar, kulit batang, dan daun tanaman N. altissima. Bakteri endofit diisolasi dan dimurnikan menggunakan medium Nutrient Agar NA dan Luria Bertani LB agar. Aktinomisetes endofit diisolasi dan dimurnikan menggunakan medium Starch Casein Agar SCA dan International Streptomyces Project ISP 2 agar. Identifikasi bakteri dan aktinomisetes endofit dilakukan secara molekuler dengan melakukan analisis filogenetik sekuen nukleotida bakteri dari daerah 16S rRNA dengan metode Neighbour Joining NJ . Isolasi dan purifikasi senyawa dilakukan dengan metode maserasi menggunakan pelarut etil asetat dan kromatografi kolom. Senyawa bioaktif dideteksi dengan teknik Kromatografi Lapis Tipis KLT bioautografi. Senyawa bioaktif yang dihasilkan oleh bakteri dan aktinomisetes endofit diidentifikasi dengan menggunakan KLT, spektroskopi Resonansi Magnetik Inti NMR dan Spektroskopi Massa LC-MS . Mekanisme aksi dari senyawa bioaktif antidiare dianalisis dengan menggunakan mikroskop elektron scanning SEM . Dari 185 isolat bakteri endofit yang diperoleh, 104 isolat 56,21 dari bagian daun; 51 isolat 27,56 dari bagian kulit batang; dan 30 isolat 16,21 dari bagian akar. Sedangkan dari 33 isolat aktinomisetes endofit yang diperoleh, dua isolat 6,06 dari bagian kulit batang, 31 isolat 93,94 dari bagian akar, dan tidak diperoleh isolat aktinomisetes dari daun. Spesies bakteri endofit potensial ialah Pseudomonas aeruginosa strain UICC B-40, P. aeruginosa strain UICC B-93, dan P. azotoformans strain UICC B-91. Sedangkan aktinomisetes endofit potensial diidentifikasi sebagai Streptomyces sp. strain UICC B-92 dan Nonomuraea sp. strain UICC B-94. Hasil identifikasi senyawa menunjukkan bahwa senyawa bioaktif yang diperoleh dari P. aeruginosa strain UICC B-40 diduga merupakan senyawa metabolit baru, terdiri atas 2E,5E -phenyl tetradeca-2,5-dienoate C20H28O2 . Senyawa bioaktif aktinomisetes Streptomyces sp. strain UICC B-92 ialah 4-O-glucocyl, 1-carboxyl-phenazine C19H18N2O8 . Senyawa turunan phenazine dengan adanya gugus gula dari isolat Streptomyces sp. strain UICC B-92 diduga merupakan senyawa bioaktif baru. Hasil bioassai aktivitas antibakteri menunjukkan baik senyawa bioaktif dari P. aeruginosa strain UICC B-40 maupun Streptomyces sp. strain UICC B-92 menghambat bakteri Gram positif Bacillus cereus strain ATCC 10876 dan Staphylococcus aureus strain ATCC 25923. Mekanisme penghambatan dari kedua senyawa menunjukkan adanya aktivitas lisis terhadap membran sel bakteri uji, ditunjukkan dengan terjadinya pemanjangan ukuran sel, kerusakan dan kebocoran membran sel sehingga mengganggu permeabilitas membran sel dan akhirnya menyebabkan kematina sel. Senyawa metabolit P. aeruginosa strain UICC B-40 lebih potensial sebagai senyawa antidiare dibandingkan senyawa metabolit dari Streptomyces sp. strain UICC B-92.Kata kunci : antidiare, bakteri endofit, 16S rRNA, lisis, Neesia altissima, spektroskopi.

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The aims of this study were to obtain potential endophytic bacteria and actinomycetes from N. altissima as anti diarrhea bioactive producer and to screen and identify their anti diarrhea bioactive compound and to investigate the mechanism of action of the bioactive compound in inhibiting the growth of diarrhea causing bacteria. Media for endophytic bacteria isolation and purification were NA and LB agar, while media for endophytic actinomycetes isolation and purification were SCA and ISP2 agar. Identification of endophytic bacteria and actinomycetes was carried out based on phylogenetic analysis of DNA sequence generated from 16S rRNA region. Isolation, purification, and detection of bioactive compounds were carried out using maceration process, column chromatography and Thin Layer Chromatography TLC bioautography, respectively. Identification were elucidated using Nuclear Magnetic Resonance NMR and Liquid Chromatography Mass Spectroscopy LC MS analyses. The mechanism of action of bioactive compound were morphologically observed using scanning electron microscope SEM . In this study, from a total 185 endophytic bacteria obtained, 104 isolates 56.21 obtained from leaves, 30 isolates 16.21 from roots, and 51 isolates 27.56 from stem barks. From a total 33 endophytic actinomycetes isolates obtained, 31 isolates 93.94 from roots, two isolates 6.06 from stem barks, and no isolates obtained from leaves. Based on phylogenetic analysis of nucleotide sequence generated from 16S rRNA region, two isolates of endophytic bacteria determined as P. aeruginosa strain UICC B 40 and one isolate belongs to P. azotoformans strain UICC B 91 two isolates of endophytic actinomycetes determined as Streptomyces sp. strain UICC B 92 and Nonomuraea sp. strain UICC B 94 . On the basis of 1H NMR spectral data and supported with molecular weight data from LC MS analysis, bioactive compound from P. aeruginosa strain UICC B 40 was identified as growth associated metabolite, and determined as 2E,5E phenyl tetradeca 2,5 dienoate C20H28O2 . In addition, bioactive compound from Streptomyces sp. strain UICC B 92 was identified as 4 O glucocyl, 1 carboxyl phenazine C19H18N2O8 . The bioactive compound from Streptomyces sp. strain UICC B 92 is suggested as novel type of phenazine derivative. All of bioactive compounds showed high in vitro antibacterial activity against two Gram positive bacteria, Bacillus cereus strain ATCC 10876 and Staphylococcus aureus strain ATCC 25923. The bioactive compounds from P. aeruginosa strain UICC B 40 and Streptomyces sp. strain UICC B 92 showed membrane cell walls lysis mechanism. The cell walls of S. aureus strain ATCC 25923 and B. cereus strain ATCC 10876 were apparently damaged after treated by the antibacterial compound. Occurrence of local rupture or pore formation in the cell membranes was also found and causing leakage of essential intracellular constituents from the cells. The bioactive compound from P. aeruginosa strain UICC B 40 is more potential as anti diarrhea compound than that from Streptomyces sp. strain UICC B 92.Key words antidiarrhea, endophyte bacteria, 16S rRNA, lysis, Neesia altissima, spectroscopy."

"Konsorsium bakteri lokal (gabungan Salipiger bermudensis DQ 178660, Alterierythrobacter evoxidivorans DQ 304436, Alteromonas macleodii Y 18228 dan Vibrio harveyi DQ 146936) pendegradasi senyawa hidrokarbon kontaminan yang diisolasi dari kawasan eksplorasi minyak Cepu Jawa Tengah diuji kemampuannya dalam merombak senyawa hidrokarbon minyak bumi yang mencemari tanah di kawasan industri Krakatau Steel Cilegon. Dalam penelitian ini, karakterisasi produksi biosurfaktan yang dihasilkan konsorsium bakteri dilakukan dengan mengevaluasi pola pertumbuhan, analisis tegangan permukaan, analisis tegangan antarmuka, analisis komposisi kimia dan uji aktivitas emulsifikasi. Pengujian selama 30 hari pengamatan meliputi pH, suhu, tekstur tanah empat fraksi (berpasir, liat kasar, liat halus, berdebu), karbon organik, nitrogen organik, rasio karbon/nitrogen organik, fosfor dan kalium serta analisis sampel tanah tercemar hidrokarbon menggunakan Gas Chromatography-Mass Sphectroscopy (GC-MS). Hasil penelitian menunjukkan bahwa biosurfaktan yang dihasilkan konsorsium bakteri memiliki kemampuan menurunkan tegangan permukaan air lebih tinggi dibanding dengan bakteri tunggal (51 dynes/cm dari 72 dyns/cm), reduksi nilai tegangan antarmuka air dengan minyak paling tinggi dihasilkan konsorsium bakteri (10 dynes/cm), nilai indeks emulsifikasi (93,75%) paling tinggi dihasilkan oleh konsorsium bakteri. Analisis komposisi kimia biosurfaktan yang dihasilkan konsorsium bakteri menunjukkan bahwa biosurfaktan merupakan senyawa kompleks terdiri dari karbohidrat, protein dan lipid. Setelah 30 hari massa inkubasi, hasil analisis GC-MS menunjukkan bahwa bakteri dan konsorsium bakteri mampu merombak senyawa hidrokarbon tersisa yang mencemari tanah di kawasan PT Krakatau Steel Cilegon Banten.;

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Local bacterial consortium (combined of Salipiger bermudensis DQ 178 660, Alterierythrobacter evoxidivorans DQ 304 436, Alteromonas macleodii Y 146 936 and Vibrio harveyi DQ 18228) hydrocarbons degrading contaminants that isolated from oil exploration areas in Cepu Central Java was analyzed for its ability to degrade petroleum hydrocarbons that polluted the soil in industrial area of PT. Krakatau Steel Cilegon.

In this study, characterization of biosurfactant produced by bacterial consortium conducted to evaluate growth patterns, analysis of surface tension, interfacial tension, chemical composition and emulsification activity assay. Analysis for 30 days of observation include pH, temperature, soil texture four fractions (sandy, dusty, rough clayey, smooth clayey), organic carbon, organic nitrogen, the ratio of carbon/nitrogen organic, phosphorus and potassium as well as analysis of hydrocarbon contaminated soil samples using Gas Chromatography -Mass Sphectroscopy (GC-MS).

The results showed that the biosurfactants produced by bacterial consortium have the ability to lower the surface tension of water is higher than with a single bacterium (51 dynes/cm from 72 dyns/cm), the reduction of the highest values ​​of water interfacial tension with oil produced by bacterial consortium (10 dynes/cm ), the highest value of emulsification index (93.75%) produced by bacterial consortium. Analysis of the chemical composition of biosurfactants produced by bacterial consortium showed that biosurfactants are complex compounds composed of carbohydrates, proteins and lipids. After 30 days of incubation time, the results of GC-MS analysis showed that bacteria and bacterial consortium are capable of overhauling the remaining hydrocarbon compounds that polluted the soil in the area of PT Krakatau Steel Cilegon Banten."