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Thesis Supervisors: Dr. Suyanto Pawiroharsono; Prof. Dr. Indrawati GandjarSUMMARYFood deterioration is often due to lipid oxidation, excluding bacterial andenzymatic spoilage. The end-products of lipid oxidation, such as aldehydes,ketones, and alcohols are responsible for unacceptable off-tiavors and off-odors in food. Lipid oxidation can be inhibited by antioxidants.Soybean tempe is the most popular indigenous fermented food inIndonesia. Soybeans are known to contain isotiavones. Four major forms areknown respectively as acetylglycosides, malonylglycosides, glycosides, andaglycones.Tempe were produced from soybean fermentation by Rhizopus oryzaeUICC 524 and Rhizopus microsporus var. chinensis UICC 521. The tempesamples were extracted with methanol and the extraction defatted with hexa»ne. The isoflavone aglycones were isolated using column chromatography,and then anaiyzed using a gradient elution reverse phase of high-pressureliquid chromatography (HPLC). After HPLC analysis, isotiavone aglyconeswere evaporated to dryness and added to soybean oil at the 100, 200, 300,iii ' and 400-ppm concentration in test tubes, then heated at 170°C for 30 minu-tes. The oxidation of soybean oil was measured using the thiobarbituric acid(TBA) test. The result, called thiobarbituric acid reactive substances(TBARS) value, was expressed as pmolll and compared to the syntheticantioxidant, buthylated hidroxytoluene (BHT), at the same concentration.The profile of isoflavone aglycones isolated contains daidzein andgenistein. No factor-2 (6,7,4'-trihidroxyisoflavone) and glycitein were detec-ted. Daidzein resulted from biotransformation of daidzin was dominant inboth tempe samples. The isoflavone biotransformation was much greater byR. microsporus var. chinensis UICC 521 than by R. oryzae UICC 524, exceptfor the 24 hours incubation. After 72 hours of incubation, the total isoflavoneaglycones in tempe using R. microsporus var. chfnensis UICC 521 was721.6 pglg and when using R. oryzae UICC 524, 268.2 p.glg.The oxidized soybean oil without any antioxidants had a TBARS value of327.32 1 20.31 pmol/1. Addition of the antioxidants showed a decreasedTBARS value following increasing concentration for both. For concentrationuntil 300 ppm, the TBARS values of oxidized soybean oil added with isofla-vone aglycones were greater than when added with BHT, respectively55.40 zl: 2.77 pmol!! and 45.20 i 2.63 pmolll. However at concentration of400 ppm, the TBARS values of oxidized soybean oil added with isoftavoneaglycones and added with BHT did not show a significant difference.ix + -51 pp; 6 append; 5 plates; 3 tables.Bilb 35 (1964-1999).iv |
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