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"Teripang telah diketahui banyak memiliki manfaat biologis, seperti antikanker, antifungal, antivirus, dan antioksidan. Penelitian dilakukan untuk mengetahui aktivitas antioksidan dan mendeteksi keberadaan senyawa saponin pada ekstrak kasar Holothuria atra (Echinodermata) dan fraksi-fraksinya. Senyawa radikal bebas DPPH digunakan untuk pengujian aktivitas antioksidan sedangkan uji busa digunakan untuk mendeteksi keberadaan senyawa saponin. Hasil penelitian menunjukkan bahwa ekstrak kasar H. atra mengandung saponin dan memiliki aktivitas antioksidan yang lebih rendah dari pembandingnya, Acanthaster sp. (Echinodermata) dengan nilai IC50 masing-masing sebesar 739,194 μg/ml dan 102,946 μg/ml. Fraksi n-heksan, fraksi etil asetat, dan fraksi air memiliki aktivitas antioksidan yang kurang kuat dengan nilai IC50 secara berurutan 511,35 μg/ml, 373,776 μg/ml, dan 491,8 μg/ml. Uji saponin terdeteksi positif pada semua fraksi kecuali fraksi etil asetat.

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Sea cucumber had been known for having many biological uses, such as anticancer, antifungal, antivirus, and antioxidant. This study was conducted to test the antioxidant activity and to detect the presence of saponin compounds in Holorhuria atra (Echinodermata) crude extract and its fractions. Free radical compound, DPPH, was used to test the antioxidant activity and foam test was used to detect the presence of saponin compounds. The result showed that crude extract of H. atra contains saponins and has weaker antioxidant activity than Acanthaster sp. (echinoderm). The IC50 values are 739,194 μg/ml and 102,946 μg/ml, respectively. N-hexane fraction, ethyl acetate fraction, and water fraction have weak antioxidant activities with IC50 values 511,35 μg/ml, 373,776 μg/ml, and 491,8 μg/ml, respectively. Saponin test showed that all of the crude extract fractions showed positive results, except in ethyl acetate fraction."

"Uji aktivitas antivirus ekstrak etanol daun angsana (Pterocarpus indicus Willd.) menyatakan bahwa daun angsana berpotensi sebagai media pengobatan demam berdarah. Penelitian bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol daun angsana dengan tingkatan dosis 0,5 , 5, 50, dan 500 mg/kg bb terhadap morfologi fetus mencit galur DDY. Bahan uji diberikan secara oral sejak hari ke-6 hingga hari ke-15 kebuntingan. Induk mencit dikorbankan dan dibedah caesar pada hari ke-18 kebuntingan. Pengamatan meliputi jumlah corpus luteum, jumlah kematian dan resorpsi, jenis kelamin fetus, penimbangan berat badan fetus, pengukuran panjang fetus, serta pengamatan visual terhadap kelengkapan morfologi fetus. Hasil uji anava (P>0,05) menyatakan bahwa pemberian ekstrak etanol daun angsana tidak memengaruhi jumlah implantasi, berat dan panjang badan fetus, serta jenis kelamin fetus. Hasil uji Kruskal-Wallis (P>0,05) menunjukkan bahwa tidak ada pengaruh nyata terhadap jumlah fetus hidup, mati, resorpsi dan kelainan morfologi, sedangkan hasil uji Mann-Whitney (P>0,05) terhadap berat plasenta menunjukkan ada pengaruh nyata akibat pemberian ekstrak etanol daun angsana.

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Antiviral activity of ethanol extract of angsana leaves (Pterocarpus indicus Willd.) showed that angsana potentially as dengue fever treatment. This study was performed to examine the influence of ethanol extract of angsana leaves at dose level 0,5 , 5, 50, and 500 mg/kg bw to mice fetus DDY strain. Substance test given by oral at 6th until 15th gestation period. At the 18th gestation, mice were euthanased by caesarian sectioned. Observation include the number of corpus luteum, number of death and resorption, weight body and crown-rump of fetus, and observation to completeness of morphological visually.

Anava test (P>0,05) showed that ethanol extract of angsana leaves did not influence the implantation number, fetus weight and crown-rump, and fetus sex. Kruskal-Wallis test (P>0,05) showed that there is no significant difference on alive, dead, and resorption fetus; and morphological abnormality, whereas Mann-Whitney test (P>0,05) on placenta weight showed there is significant difference due to administration of ethanol extract of angsana leaves."

"Pemanfaatan biota laut di bidang kesehatan salah satunya adalah sebagai sumber antioksidan yang dapat menangkal radikal bebas. Penelitian dilakukan untuk menguji aktivitas antioksidan dan mendeteksi keberadaan senyawa terpenoid pada ekstrak kasar Acanthaster (Echinodermata) dan fraksi-fraksinya. Metode DPPH digunakan untuk pengujian aktivitas antioksidan sedangkan pelarut Libermann Burchard digunakan untuk mendeteksi keberadaan senyawa terpenoid. Hasil penelitian menunjukkan bahwa ekstrak kasar metanol Acanthaster mengandung terpenoid dan memiliki aktivitas antioksidan yang lebih kuat dibandingkan pembandingnya, Didemnum sp. (Ascidia) dengan nilai IC50 yaitu masing-masing sebesar 102,946 μg/ml dan 118,373 μg/ml. Fraksi etil asetat, fraksi n-heksan, dan fraksi air memiliki aktivitas antioksidan yang kuat dengan nilai IC50 secara berurutan 74,481 μg/ml, 100,084 μg/ml, dan 194,652 μg/ml. Aktivitas antioksidan fraksi etil asetat dan fraksi n-heksan disebabkan oleh terpenoid, sedangkan aktivitas antioksidan fraksi air disebabkan oleh senyawa lain yang bersifat polar karena menunjukkan hasil negatif pada uji terpenoid.

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Many marine organism have been known as source of antioxidant that could counteract radical ions. The study was conducted to test the antioxidant activity and to detect the presence of terpenoid compounds in the Acanthaster (echinoderm) crude extract and its fractions. DPPH method was used to test the antioxidant activity and Liebermann Burchard reagent was used to detect the presence of terpenoid compounds. The result shown that crude extract of Acanthaster contained terpenoids and has stronger antioxidant activity than ascidian Didemnum sp. (IC50 values are 102.946 μg/ml and 118.373 μg/ml, respectively). Ethyl acetate fraction, n-hexane fraction, and water fraction have strong antioxidant activities with IC50 values 74.481 μg/ml, 100.084 μg/ml, dan 194.652 μg/ml, respectively. The antioxidant activities of ethyl acetate fraction and n-hexane fraction were caused by terpenoids, whereas the antioxidant activity of water fraction was caused by other polar antioxidant compounds because has negative result of terpenoid test.

Pemanfaatan biota laut di bidang kesehatan salah satunya adalah sebagai sumber

antioksidan yang dapat menangkal radikal bebas. Penelitian dilakukan untuk

menguji aktivitas antioksidan dan mendeteksi keberadaan senyawa terpenoid pada

ekstrak kasar Acanthaster (Echinodermata) dan fraksi-fraksinya. Metode DPPH

digunakan untuk pengujian aktivitas antioksidan sedangkan pelarut Libermann

Burchard digunakan untuk mendeteksi keberadaan senyawa terpenoid. Hasil

penelitian menunjukkan bahwa ekstrak kasar metanol Acanthaster mengandung

terpenoid dan memiliki aktivitas antioksidan yang lebih kuat dibandingkan

pembandingnya, Didemnum sp. (Ascidia) dengan nilai IC50 yaitu masing-masing

sebesar 102,946 μg/ml dan 118,373 μg/ml. Fraksi etil asetat, fraksi n-heksan, dan

fraksi air memiliki aktivitas antioksidan yang kuat dengan nilai IC50 secara

berurutan 74,481 μg/ml, 100,084 μg/ml, dan 194,652 μg/ml. Aktivitas

antioksidan fraksi etil asetat dan fraksi n-heksan disebabkan oleh terpenoid,

sedangkan aktivitas antioksidan fraksi air disebabkan oleh senyawa lain yang

bersifat polar karena menunjukkan hasil negatif pada uji terpenoid.

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Abstract

Many marine organism have been known as source of antioxidant that could

counteract radical ions. The study was conducted to test the antioxidant activity

and to detect the presence of terpenoid compounds in the Acanthaster

(echinoderm) crude extract and its fractions. DPPH method was used to test the

antioxidant activity and Liebermann Burchard reagent was used to detect the

presence of terpenoid compounds. The result shown that crude extract of

Acanthaster contained terpenoids and has stronger antioxidant activity than

ascidian Didemnum sp. (IC50 values are 102.946 μg/ml and 118.373 μg/ml,

respectively). Ethyl acetate fraction, n-hexane fraction, and water fraction have

strong antioxidant activities with IC50 values 74.481 μg/ml, 100.084 μg/ml, dan

194.652 μg/ml, respectively. The antioxidant activities of ethyl acetate fraction

and n-hexane fraction were caused by terpenoids, whereas the antioxidant activity

of water fraction was caused by other polar antioxidant compounds because has

negative result of terpenoid test. "

"Penelitian limbah organik berbahan dasar kompos sebagai medium pertumbuhan Nannochloropsis sp. telah dilakukan. Penelitian ini bertujuan untuk mendapatkan variasi konsentrasi ekstrak kompos yang tepat dengan mengukur laju pertumbuhan dan kadar lipid Nannochloropsis sp. Sediaan ekstrak kompos dibuat dalam konsentrasi 5%, 10%, 25%, dan 50% (v/v) dari volume ekstraksi kompos 100 g/L. Penelitian dilakukan selama 240 jam. Hasil penelitian menunjukkan bahwa Nannochloropsis sp. yang dikultur pada medium ekstrak kompos 25% memiliki pertumbuhan paling tinggi dibandingkan dengan konsentrasi ekstrak kompos lainnya. Kerapatan sel maksimum pada perlakuan ekstrak kompos 25% mencapai 22,04±10,65 x 106 sel/mL pada jam ke-240 pada laju pertumbuhan spesifik maksimum μ= 0,075 per jam. Kadar dan produktivitas lipid yang diperoleh sebesar 18,31±11,96 % (w/w) dan 0,0172±0,0005 g/L/hari. Hasil tersebut menunjukkan bahwa ekstrak kompos 25% dapat dijadikan sebagai medium pertumbuhan Nannochloropsis sp.

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Research of the compost on the medium on the growth of Nannochloropsis sp. was performed. The research objective was to determine the influence of the medium on the growth and lipid content of Nannochloropsis sp. Compost extracts preparations were made in the concentration of 5%, 10%, 25%, and 50% (v/v) of the volume extraction of 100 g/L. Experiment was conducted for 240 hours.

The results showed that Nannochloropsis sp. were cultured in medium 25% compost extract had the highest growth compared to other compost extract concentration. The maximum cell density at 25% compost extract treatment reached 22.04±10.65 x 106 sel/mL at 240 hours with a maximum specific growth rate μ = 0.075 /hours Meanwhile, lipid content and productivity were obtained by 18.31±11.96 % (w/w) and 0.0172±0.0005 g/L/day. These results indicate the compost can be used as a medium for the growth of Nannochloropsis sp."

"Biology courses in senior high chool can play an important role in the effort to improve the student critical thinking skill. ..."

"Pola sebaran dan perilaku bintang laut intertidal di Teluk Gilimanuk dan Pantai Cekik diteliti dari September 2012 hingga Januari 2013. Pola sebaran dipetakan dengan menggunakan GPS, dan dikuantifikasikan dengan menggunakan kuadrat 1 m2, yang diletakkan pada total 12 transek untuk masing-masing lokasi. Perilaku diamati dengan menandakan 10 individu Protoreaster nodosus secara manual dan mengukur titik perpindahan selama tiga hari. Pemetaan dan analisis nilai dispersi dengan menggunakan Indeks dispersi Morisita terstandarisasi menghasilkan pola sebaran mengelompok untuk Protoreaster nodosus dan Archaster typicus, dan pola sebaran acak untuk Echinaster luzonicus, Linckia laevigata dan Linckia multifora. Sebaran mengelompok tersebut dapat disebabkan oleh batasan habitat, sebaran makanan dan atraksi intraspesifik, sedangkan sebaran acak menandakan sifat acak atau seragam pada pakan dan karakter habitat. Studi perilaku memperlihatkan bahwa P. nodosus bergerak secara direksional, dan dapat berpengaruh pada pola sebarannya di lokasi.

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Distribution pattern and behavior of intertidal asteroids in Gilimanuk Bay and Cekik Beach were investigated from September 2012 to January 2013. Distribution patterns for most species were mapped by using GPS, and quantified by using 1 m2 quadrats, which were deployed on total 12 transects in the intertidal zone at each location. Behavior was observed by using manual tagging on ten individuals of Protoreaster nodosus and measuring points of diplacement throughout a period of three days. Mapping and dispersion analysis using Morisita‟s standardized index of dispersion yielded clumped dispersion for Protoreaster nodosus and Archaster typicus, and random dispersion for Echinaster luzonicus, Linckia laevigata and Linckia multifora. Clumped dispersion in some species might be influenced by habitat boundaries, food dispersion or intraspecific attraction, while random dispersion suggests randomness or uniformity in food distribution and habitat character. Behavioral studies showed that P. nodosus moves in a highly directional manner, and might carry further implications to its clumped dispersion at location. "

"ABSTRAKPreservasi koleksi kultur Nostoc dilakukan mengingat Nostoc merupakan salah satu mikroalga tanah yang penting dan berguna. Metode preservasi yang umum digunakan untuk Nostoc adalah dengan subkultur berkala dan freezing. Delapan strain Nostoc yang digunakan dalam preservasi dengan metode freezing pada suhu (-80oC) adalah CPG8, CPG24, BAD5, GIA13a, GIA12-03, TAB7d, BTM6-01 dan TAK23. Penambahan protektan intraseluler DMSO 5% dilakukan untuk mengurangi efek dari proses freezing (cold stress) pada Nostoc. Sebagai pembanding, delapan strain tersebut juga dipreservasi tanpa menggunakan protektan (kontrol negatif) dalam medium BG 11 bebas unsur nitrogen, kemudian dipreservasi dalam deep freezer (-80oC) selama 7 hari (H7) dan 90 hari (H90). Hasil menunjukkan terdapat variasi respon tumbuh strain-strain Nostoc setelah dipreservasi. Strain yang dipreservasi menggunakan protektan DMSO 5% menunjukkan selisih diameter koloni yang lebih besar, kerusakan filamen lebih sedikit dan kadar klorofil lebih tinggi dibandingkan kontrol negatif.

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ABSTRACTPreservation of culture collections Nostoc done considering that Nostoc is one of microalgae soil that important and useful. Preservation methods commonly used for Nostoc is with periodic subculture, but that methods have short comings vulnerable to loss of genetic stability. Beside that, freezing method is commonly used for Nostoc preservation. Eight Nostoc strains used in preservation by freezing method at temperature -80oC are CPG8, CPG24, BAD5, GIA13a, GIA12- 03, TAB7d, BTM6-01 and TAK23. The addition of the intracellular protectant DMSO 5 % was done to decrease the effects of freezing (cold stress) in Nostoc. For comparison, eight strains were also preserved without using protectant (negative control) in BG 11 nitrogen free medium, then preserved in a deep freezer (-80oC) for 7 days (H7) and 90 days (H90). Results showed variation in response to growing strains Nostoc after preservation. Strains were preserved using DMSO 5% protectant showed difference of colony diameter, filament has less damage and has higher clorophyl value compared with negative control."

"Telah dilakukan penelitian mengenai struktur komunitas dan sebaran spasial Bivalvia serta hubungannya dengan karakteristik lingkungan di Teluk Katania, Seram Barat, Maluku Tengah. Ada 5 stasiun penelitian yaitu : Pelita Jaya 1, Pelita Jaya 2, Pulau Buntal, Pulau Tatumbu, dan Pulau Burung. Anadara maculafa merupakan jenis Bivalvia yang kepadatannya tertinggi (2,5 individu/m2). Nilai H' (indeks keanekaragaman) Bivalvia tertinggi ada di Pulau Burung (H' = 0,958) dan terendah di Pulau Buntal (H' = 0,624). Indeks kemerataan J' tertinggi (J' = 0,843) terdapat di Pelita Jaya 1. indeks kesamaan Morisita C tertinggi adalah antara Pelita Jaya 2 dan Pulau Buntal (C = 0,92). Kondisi substrat di Pelita Jaya 2 dan Pulau Buntal mempunyai persentase Lumpur yang tinggi. Dengan analisis cluster, 5 stasiun penelitian terbagi menjadi 2 kelompok. Kelompok I terdiri atas stasiun Pelita Jaya 2 dan Pulau Buntal kemudian kelompok II terdiri atas Pelita Jayal, Pulau Tatumbu, dan Pulau Burung. Analisis diskriminan dengan faktor lingkungan substrat pasir halus dan lumpur juga membagi kelima stasiun menjadi dua kelompok yang sama seperti pada analisis cluster. Substrat lumpur mempunyai kontribusi yang tinggi (96,3 %). Tellina sp merupakan jenis yang penyebarannya luas sebab selalu hadir di setiap stasiun penelitian. Hasil analisis faktorial koresponden membentuk enam kelompok. Penyebaran spasial jenis jenis Bivalvia di 5 stasiun dari hasil analisis tersebut berdasarkan pada kepadatan tertinggi dari jenis jenis tertentu (lima kelompok) dan berdasarkan jenis-jenis yang selalu hadir di setiap stasiun (satu kelompok).

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Bivalvia, also known as Pelecypoda, is the second largest class in phylum Mollusca. This group has 28.000 species (Barth & Broshear, 1982) and about 1000 species of Bivalvia live in Indonesian waters (Nontji, 1987). Information about Bivalvia in Kotania Bay has not been known well. Based on those fact, a research on the community structure and spatial distribution of Bivalve in the waters of Kotania Bay was conducted in February 1996. The aim of study was to find out the relationship between Bivalvia community structure with environmental factors in the waters of Kotania Bay. The spatial distribution of Bivalvia in several small islands in Kotania Bay was also studied. Hopefully, the results can be used as basic information for father research.

The research was conducted in five stations, i.e. Pelita Jaya 1, Pelita Jaya 2, Buntal Island, Tatumbu Island, and Burung Island. The sampling method used in the study was belt transact. The water conditions measured were water temperature, salinity, and pH. The substrates were characterized by the sediment fractions. Temperatures in the research stations ranged between 29.5°C and 31 °C, the range of pH is between 7 and 8.4. Water salinities in the research stations varied between 22 % and 30 %. Substrates in the research stations mostly contained sand with the very high percentage of very coarse sand. Silt was the smallest fraction found in the substrate.

In five research stations, 32 species of Bivalvia belonging to 15 families were collected. Anadara maculata had the highest density (2.5 individulm2) of all Bivalvia species found. The highest H' value (diversity index) of Bivalvia was in Burung Island (H' = 0.958) and the lowest was in Buntal Island (H' = 0.624). The highest evenness index J' = 0.843 was found in Pelita Jaya 1. The highest similarity index was shown by Pelita Jaya 2 and Buntal Island. Substrates in Pelita Jaya 2 and Buntal Island were dominated by silt.

Cluster analysis at five research stations divided the stations into two groups. Group I, defined by Pelita Jaya 2 and Buntal Island. Group ii, defined by Pelita Jaya 1, Tatumbu Island, and Burung Island. Discriminant analysis based on two environmental factors, i.e. very fine sand and silts, also divided the stations into two similar groups as cluster analysis did. The silt factor had high contribution (96.3%) in separating the stations.

Factorial correspondence analysis classified the species of Bivalvia into six groups. Based on the highest density of the certain species (five groups) and on the common species found in all stations (one group). Among the species collected Tellina sp was the common species found in the five stations. Spondylus squamosus, Trachydarium subrugosum, and Vulsella vulsella were found only in Pelita Jaya 2. Pitar subpellucidus, Septifer hi/ocular-is, Fimbria fmbriata and Chama pacifica were found only in Burung island. The species of Bivalvia only found in Pelita Jaya 1 were Atrina vexillum, Tellina staurella, Chama ruderalis, Limaria fragilis, and Clycymeris pectunculus."