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Nova Elvira
Analisis tipe strain dan jumlah bakteri enterococcus faecalis pada lesi endo-perio kombinasi murni = Analysis of strain type and quantitatif of enterococcus faecalis bacteria in true combined endo-perio lesions
"ABSTRAK
Latar Belakang: E.faecalis merupakan bakteri saluran akar yang dapat
bermigrasi ke jaringan periodonsium pada lesi endo-perio kombinasi murni.
Jumlahnya berperan terhadap keparahan penyakit karena sifat virulensinya.
Tujuan: Menganalisis tipe strain dan jumlah E.faecalis di saluran akar dan poket
lesi endo-perio kombinasi murni, lesi endo primer, dan lesi perio primer. Metode:
16 sampel dari 3 kelompok, analisis strain dengan sequencing dan kuantifikasi
dengan Real Time PCR. Hasil: Terdapat persamaan strain E.faecali,s jumlah
terbesar terdapat pada poket lesi endo-perio kombinasi murni. Tidak berbeda
bermakna antara saluran akar dan poket pada lesi endo-perio kombinasi murni.
Kesimpulan; terdapat strain yang sama dan jumlah E.faecalis sebanding antara
saluran akar dan poket lesi endo-perio kombinasi murni.ABSTRACT
Background: E.faecalis is a root canal pathogen that can migrate to periodontal
tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part in
the severity of the disease because of its virulence. Objective: To analyze the
strain type and quantity of E.faecalis taken from root canal and pocket of true
combined endo-perio lesion, primary endodontic lesion and primary periodontal
lesion. Methods: 16 samples were taken from 3 groups, strain type was analyzed
by sequencing and quantification by Real Time PCR. Results: There were
similarities of E. faecalis strain. The largest number were found in the pockets of
true combined endo-perio lesion. There were no significant differences between
root canal and pocket of true combined endo-perio lesions. Conclusion: There
were similarities strain and quantity of E. faecalis were equal between root canal
and pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal
tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part in
the severity of the disease because of its virulence. Objective: To analyze the
strain type and quantity of E.faecalis taken from root canal and pocket of true
combined endo-perio lesion, primary endodontic lesion and primary periodontal
lesion. Methods: 16 samples were taken from 3 groups, strain type was analyzed
by sequencing and quantification by Real Time PCR. Results: There were
similarities of E. faecalis strain. The largest number were found in the pockets of
true combined endo-perio lesion. There were no significant differences between
root canal and pocket of true combined endo-perio lesions. Conclusion: There
were similarities strain and quantity of E. faecalis were equal between root canal
and pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal
tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part in
the severity of the disease because of its virulence. Objective: To analyze the
strain type and quantity of E.faecalis taken from root canal and pocket of true
combined endo-perio lesion, primary endodontic lesion and primary periodontal
lesion. Methods: 16 samples were taken from 3 groups, strain type was analyzed
by sequencing and quantification by Real Time PCR. Results: There were
similarities of E. faecalis strain. The largest number were found in the pockets of
true combined endo-perio lesion. There were no significant differences between
root canal and pocket of true combined endo-perio lesions. Conclusion: There
were similarities strain and quantity of E. faecalis were equal between root canal
and pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal
tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part in
the severity of the disease because of its virulence. Objective: To analyze the
strain type and quantity of E.faecalis taken from root canal and pocket of true
combined endo-perio lesion, primary endodontic lesion and primary periodontal
lesion. Methods: 16 samples were taken from 3 groups, strain type was analyzed
by sequencing and quantification by Real Time PCR. Results: There were
similarities of E. faecalis strain. The largest number were found in the pockets of
true combined endo-perio lesion. There were no significant differences between
root canal and pocket of true combined endo-perio lesions. Conclusion: There
were similarities strain and quantity of E. faecalis were equal between root canal
and pocket true combined endo-perio lesions. "
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2015
SP-PDF
UI - Tugas Akhir  Universitas Indonesia Library
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Putie Ambun Suri
Analisis jumlah dan proporsi bakteri porphyromonas gingivalis pada lesi endo-perio kombinasi murni = Analysis of quantity and proportion of porphyromonas gingivalis bacteria in true combined endo-perio lesions
"ABSTRAK
Latar Belakang. P.gingivalis merupakan bakteri periodonsium yang dapat
berpindah ke saluran akar pada lesi endoperio kombinasi murni. Jumlah dan
proporsinya berperan terhadap keparahan penyakit karena sifat virulensinya.
Tujuan: Menganalisis jumlah dan proporsi P.gingivalis saluran akar dan poket
lesi endoperio kombinasi murni, lesi endo primer, dan lesi perio primer. Metode:
16 sampel diambil dari 3 kelompok, kuantifikasi dengan PCR real-time. Hasil:
Jumlah P.gingivalis terbesar pada poket lesi perio primer, proporsi P.gingivalis
terbesar pada saluran akar lesi endo primer. Jumlah dan proporsi P.gingivalis
tidak berbeda bermakna antara saluran akar dan poket lesi endoperio kombinasi
murni. Kesimpulan. Jumlah dan proporsi P.gingivalis sebanding antara saluran akar dan poket lesi endoperio kombinasi murni.
ABSTRACT
Background : P.gingivalis is a periodontium pathogen that can be transferred to
the root canal of the true combined endoperio lesions. Quantity and proportion
takes part in the severity of the disease because of its virulence. Objective: to
analyze the quantity and proportion of P.gingivalis in the root canals and pocket
of true combined endoperio lesions, primary endodontic lesions, and primary
periodontal lesions. Methods 16 samples were taken from 3 groups,
quantification with real-time PCR. Results : P.gingivalis largest quantity was
found in pockets of primary periodontal lesions, P.gingivalis largest proportion
was found in root canals of primary endodontic lesions. There were no significant
differences in quantity and proportion of P.gingivalis in root canals and pockets of
true combined endoperio lesions. Conclusion. Quantity and proportion of
P.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.
;Background : P.gingivalis is a periodontium pathogen that can be transferred to
the root canal of the true combined endoperio lesions. Quantity and proportion
takes part in the severity of the disease because of its virulence. Objective: to
analyze the quantity and proportion of P.gingivalis in the root canals and pocket
of true combined endoperio lesions, primary endodontic lesions, and primary
periodontal lesions. Methods 16 samples were taken from 3 groups,
quantification with real-time PCR. Results : P.gingivalis largest quantity was
found in pockets of primary periodontal lesions, P.gingivalis largest proportion
was found in root canals of primary endodontic lesions. There were no significant
differences in quantity and proportion of P.gingivalis in root canals and pockets of
true combined endoperio lesions. Conclusion. Quantity and proportion of
P.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.
;Background : P.gingivalis is a periodontium pathogen that can be transferred to
the root canal of the true combined endoperio lesions. Quantity and proportion
takes part in the severity of the disease because of its virulence. Objective: to
analyze the quantity and proportion of P.gingivalis in the root canals and pocket
of true combined endoperio lesions, primary endodontic lesions, and primary
periodontal lesions. Methods 16 samples were taken from 3 groups,
quantification with real-time PCR. Results : P.gingivalis largest quantity was
found in pockets of primary periodontal lesions, P.gingivalis largest proportion
was found in root canals of primary endodontic lesions. There were no significant
differences in quantity and proportion of P.gingivalis in root canals and pockets of
true combined endoperio lesions. Conclusion. Quantity and proportion of
P.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.
;Background : P.gingivalis is a periodontium pathogen that can be transferred to
the root canal of the true combined endoperio lesions. Quantity and proportion
takes part in the severity of the disease because of its virulence. Objective: to
analyze the quantity and proportion of P.gingivalis in the root canals and pocket
of true combined endoperio lesions, primary endodontic lesions, and primary
periodontal lesions. Methods 16 samples were taken from 3 groups,
quantification with real-time PCR. Results : P.gingivalis largest quantity was
found in pockets of primary periodontal lesions, P.gingivalis largest proportion
was found in root canals of primary endodontic lesions. There were no significant
differences in quantity and proportion of P.gingivalis in root canals and pockets of
true combined endoperio lesions. Conclusion. Quantity and proportion of
P.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.
;Background : P.gingivalis is a periodontium pathogen that can be transferred to
the root canal of the true combined endoperio lesions. Quantity and proportion
takes part in the severity of the disease because of its virulence. Objective: to
analyze the quantity and proportion of P.gingivalis in the root canals and pocket
of true combined endoperio lesions, primary endodontic lesions, and primary
periodontal lesions. Methods 16 samples were taken from 3 groups,
quantification with real-time PCR. Results : P.gingivalis largest quantity was
found in pockets of primary periodontal lesions, P.gingivalis largest proportion
was found in root canals of primary endodontic lesions. There were no significant
differences in quantity and proportion of P.gingivalis in root canals and pockets of
true combined endoperio lesions. Conclusion. Quantity and proportion of
P.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.
"
Fakultas Kedokteran Gigi Universitas Indonesia, 2015
SP-PDF
UI - Tugas Akhir  Universitas Indonesia Library
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Hirania Soraya
Daya antibakteri campuran triantibiotik dibandingkan dengan khlorheksidin 2% terhadap biofilm bakteri enterococcus faecalis dari gigi non vital dengan kelainan periapeks = Antibacterial effectivity of triantibiotic mixture compared to 2% chlorhexidine against biofilm of enterococcus faecalis from tooth with periapical lesion
"ABSTRAK
Latar Belakang : Enterococcus faecalis merupakan bakteri yang mampu membentuk biofilm dan banyak ditemukan pada kasus kelainan periapeks.
Tujuan : Melihat daya antibakteri campuran triantibiotik dibandingkan dengan
klorheksidin 2% terhadap E. faecalis dalam biofilm. Metode : Menilai kekeruhan
larutan E. faecalis dalam biofilm pasca pemaparan bahan uji, dengan ELISA
reader. Hasil : Terdapat daya antibakteri campuran triantibiotik terhadap biofilm
bakteri E. faecalis tetapi tidak terdapat perbedaan bermakna dengan klorheksidin
2% (p>0.05). Kesimpulan : Daya antibakteri campuran triantibiotik terhadap
biofilm E. faecalis sebanding dengan klorheksidin 2%. ABSTRACT
Background : Enterococcus faecalis has the ability to form biofilm and is often
found in cases of periapical lesions. Aim: To analyze the effectivity of triantibiotic
mixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :
Score the turbidity of E. faecalis in biofilm after immersion in antibacterial
agent, with ELISA reader. Result : Triantibiotic mixture has antibacterial
effectivity against E. faecalis biofilm but has no significant difference compared to
2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibiotic
mixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is often
found in cases of periapical lesions. Aim: To analyze the effectivity of triantibiotic
mixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :
Score the turbidity of E. faecalis in biofilm after immersion in antibacterial
agent, with ELISA reader. Result : Triantibiotic mixture has antibacterial
effectivity against E. faecalis biofilm but has no significant difference compared to
2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibiotic
mixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is often
found in cases of periapical lesions. Aim: To analyze the effectivity of triantibiotic
mixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :
Score the turbidity of E. faecalis in biofilm after immersion in antibacterial
agent, with ELISA reader. Result : Triantibiotic mixture has antibacterial
effectivity against E. faecalis biofilm but has no significant difference compared to
2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibiotic
mixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is often
found in cases of periapical lesions. Aim: To analyze the effectivity of triantibiotic
mixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :
Score the turbidity of E. faecalis in biofilm after immersion in antibacterial
agent, with ELISA reader. Result : Triantibiotic mixture has antibacterial
effectivity against E. faecalis biofilm but has no significant difference compared to
2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibiotic
mixture against E. faecalis biofilm is equal to2% chlorhexidine. "
Fakultas Kedokteran Universitas Indonesia, 2015
SP-PDF
UI - Tugas Akhir  Universitas Indonesia Library
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Louis Martin
Analisis Perkembangan Gigi (Odontogenesis) pada Mencit Strain C57BL/6 Usia Satu Hari = Analysis of Teeth Development Process (Odontogenesis) in One-Day-Old C57BL/6 Mice
"Latar Belakang: Setiap tahap odontogenesis yang tidak berjalan dengan baik semestinya dapat mengakibatkan terjadinya abnormalitas pada gigi, hal tersebut dipengaruhi oleh beberapa faktor atau komponen tertentu seperti gen, nutrisi, mineral, molekul, atau lainnya. Untuk memahami proses terjadinya abnormalitas serta faktor-faktor yang memengaruhinya, perlu diketahui secara spesifik setiap tahapan perkembangan gigi. Penelitian terkait perkembangan gigi pada manusia tidak memungkinkan karena diperlukan tindakan yang invasif dalam pengambilan sampel jaringan. Oleh sebab itu, digunakan hewan coba mencit C57BL/6 karena merupakan jenis inbred dan banyak digunakan dalam penelitian biomolekuler. Namun, hingga saat ini penelitian mengenai odontogenesis pada mencit C57BL/6 masih sangat terbatas. Tujuan: Menganalisis perkembangan gigi (odontogenesis) mencit strain C57BL/6 usia satu hari. Metode: Rahang mencit C57BL/6 dipotong menjadi 4 bagian. Setelah itu, dilakukan pembuatan preparat dengan potongan longitudinal (sagital) pada maksila dan mandibula kanan dan potongan koronal (frontal) pada maksila dan mandibula kiri, serta dilakukan pewarnaan hematoksilin dan eosin (H&E) pada preparat dan dilakukan pengamatan benih gigi insisif dan molar rahang atas dan rahang bawah menggunakan mikroskop. Hasil: Terlihat benih gigi insisif, molar pertama, molar kedua, dan molar ketiga pada jaringan maksila dengan potongan transversal atau axial dan terlihat adanya benih gigi molar pada preparat jaringan maksila dan mandibula dengan potongan koronal. Pada jaringan mandibula dengan potongan longitudinal atau sagital hanya terlihat adanya benih gigi insisif. Kesimpulan: Terjadi proses odontogenesis hingga tahap awal bell stage pada gigi molar maksila kiri dan mandibula kiri mencit C57BL/6 usia satu hari, sehingga mencit C57BL/6 usia satu hari dapat dijadikan alternatif objek penelitian dalam menganalisis perkembangan struktur jaringan gigi baik pada kondisi normal maupun patologis.
Background: Disruption in the stages of odontogenesis results in teeth abnormalities. These conditions can be influenced by certain factors or components such as genes, nutrients, minerals, molecules, or others. To understand the process of abnormality and the factors that influence it, it is necessary to know specifically each stage of tooth development. Research related to the development of teeth in humans is not possible because it requires an invasive procedure in tissue sampling. Therefore, C57BL/6 mice were used as experimental animals because they are an inbred species and are widely used in biomolecular research. However, until now research on odontogenesis in C57BL/6 mice is still very limited. Objective: Analyzing tooth development (odontogenesis) in one-day-old C57BL/6 mice. Methods: The jaws of C57BL/6 mice were cut into 4 parts. After that, preparations were made with longitudinal (sagittal) sections on the right maxilla and mandible and coronal (frontal) sections on the left maxilla and mandible, and stained with hematoxylin and eosin (H&E) on the preparations and observation of the maxillary and mandibular incisors and molars using a microscope. Results: Tooth germs of incisors, first molars, second molars, and third molars were observed in the maxillary tissue with transverse or axial sections and the presence of molars in the maxillary and mandibular tissue preparations with coronal sections. In mandibular tissue with longitudinal or sagittal sections, only incisor germs were seen. Conclusion: The odontogenesis of one-day-old C57BL/6 mice in this study were observed in the early bell stage both in the left maxillary and left mandibular molars. Based on this study, one-day-old C57BL/6 mice can be used as an alternative object of research in analyzing the tooth structure that have been developed in the early bell stage, both normal and pathological conditions."
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2021
S-pdf
UI - Skripsi Membership  Universitas Indonesia Library
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Deryana Marshadhianti
Perbedaan nilai kuat rekat braket metal antara pada permukaan email gigi dan permukaan resin komposit nanohibrid = The Difference between metal bracket bond strength value on dental enamel and nanohybrid composite resins surface
"ABSTRAK Pendahuluan: Meningkatnya jumlah pasien dewasa dengan restorasi sewarna gigi, seperti resin komposit, menyebabkan perekatan braket pada permukaan artifisial gigi menjadi suatu tantangan tersendiri karena sering terjadi kegagalan rekat. Saat ini, belum ada riset yang dilakukan untuk melihat perbedaan nilai kekuatan rekat braket (baik nilai kuat geser maupun nilai kuat tarik braket) antara pada permukaan email gigi dan resin komposit nanohibrid. Metode: 32 gigi premolar bawah dibagi menjadi 4 kelompok: kelompok A1) spesimen email gigi (uji kuat rekat geser); kelompok A2) spesimen resin komposit nanohibrid (uji kuat rekat geser); kelompok B1) spesimen email gigi (uji kuat rekat tarik); kelompok B2) spesimen resin komposit nanohibrid (uji kuat rekat tarik). Braket direkatkan pada spesimen lalu diuji dengan menggunakan Universal Testing Machine Shimazu AG-5000 dalam waktu 24 jam setelah braket direkatkan. Hasil: Rerata nilai kuat rekat geser pada kelompok A1 sebesar 10.78 ± 0.13 MPa dan pada kelompok A2 sebesar 10.63 ± 0.18 MPa. Rerata nilai kuat rekat tarik pada kelompok B1 sebesar 10.74 ± 0.15 MPa dan pada kelompok B2 sebesar 10.65 ± 0.14 MPa. Pada uji statistik tidak terdapat perbedaan bermakna secara pada nilai kuat rekat geser maupun nilai kuat rekat tarik braket metal antara pada permukaan email gigi dan resin komposit nanohibrid. Kesimpulan: Tidak terdapat perbedaan bermakna secara statistik pada nilai kuat rekat geser maupun nilai kuat rekat tarik braket metal antara pada permukaan email gigi dan resin komposit nanohibrid. Seluruh kelompok memiliki nilai rerata kuat rekat geser dan nilai rerata kuat rekat tarik yang memadai untuk keperluan klinis perawatan ortodontik.
ABSTRACT
Introduction: The increasing number of adult patients with tooth-colored restorations, such as composite resins, causes the attachment of brackets on artificial surfaces of teeth to be a challenge because of frequent adhesive failures. At present, no research has been carried out to see the difference in bracket adhesive strength values (both shear bond strength and tensile bond strength) between the enamel surface and nanohybrid composite resins surface. Methods: 32 lower premolar were divided into 4 groups: group A1) dental enamel specimens for shear bond strength test; group A2) nanohybrid composite resins specimens for shear bond strength test; group B1) dental enamel specimens for tensile bond strength test; group B2) nanohybrid composite resins specimens for tensile bond strength test. The bracket was bonded to the specimens and tested using Universal Testing Machine Shimazu AG-5000 within 24 hours after the bracket was bonded to the specimens. Results: The mean value of shear bond strength in group A1 was 10.78 ± 0.13 MPa and in group A2 was 10.63 ± 0.18 MPa. The mean value of tensile bond strength in group B1 was 10.74 ± 0.15 MPa and in group B2 was 10.65 ± 0.14 MPa. In the statistical test there was no significant difference in the shear bond strength value or the tensile bond strength value between the metal bracket bonded to the enamel surfaces and to the nanohybrid composite resin surfaces. Conclusion: There was no significant difference in the shear bond strength and the tensile bonding strength value of the metal bracket bonded to the enamel surface and the nanohybrid composite resin surfaces. All groups have an adequate bond strength value for the clinical needs of orthodontic treatment."
Depok: Fakultas Kedokteran Gigi Universitas Indonesia, 2018
T-Pdf
UI - Tesis Membership  Universitas Indonesia Library
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Jennifer Fortiana
Efek penambahan steroid pada semen berbasis kalsium silikat terhadap viabilitas sel fibroblas = Effect of steroid addition in calcium silicate based cement on fibroblast cells viability
"ABSTRAK
Latar Belakang: Berbagai material kaping pulpa berbahan dasar kalsium silikat
terus dikembangkan, diantaranya semen berbasis kalsium silikat dengan
penambahan steroid. Tujuan: Menganalisis efek penambahan steroid pada semen
berbasis kalsium silikat terhadap viabilitas sel fibroblas. Metode: Sel fibroblas
embrio ayam direndam dalam ekstrak larutan semen berbasis kalsium silikat
dengan penambahan steroid dan MTA. Viabilitas sel dihitung dengan
menggunakan uji MTT. Hasil: Terdapat perbedaan bermakna (p ≤ 0,05)
viabilitas sel pada kelompok semen berbasis kalsium silikat dengan penambahan
steroid dibandingkan kelompok kontrol dan MTA. Kesimpulan: Penambahan
steroid menurunkan viabilitas sel. Terdapat peningkatan pada 72 jam, yang menandakan terjadinya proliferasi sel.ABSTRACT
Background: Calcium silicate based materials are being developed continuously,
one of them is calcium silicate based cement containing steroid. Objective: To
analyze the effect of steroid addition in calcium silicate based cement on
fibroblast cells viability. Methods: Chicken embryonic fibroblast cells were
immersed in extract solution of calcium silicate based cement containing steroid
and MTA. Viability was analyzed by MTT Assay. Results: Significant difference
(p ≤ 0,05) of viability on calcium silicate based cement containing steroid group
was found, compared to control and MTA group. Conclusion: Steroid addition
decrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously,
one of them is calcium silicate based cement containing steroid. Objective: To
analyze the effect of steroid addition in calcium silicate based cement on
fibroblast cells viability. Methods: Chicken embryonic fibroblast cells were
immersed in extract solution of calcium silicate based cement containing steroid
and MTA. Viability was analyzed by MTT Assay. Results: Significant difference
(p ≤ 0,05) of viability on calcium silicate based cement containing steroid group
was found, compared to control and MTA group. Conclusion: Steroid addition
decrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously,
one of them is calcium silicate based cement containing steroid. Objective: To
analyze the effect of steroid addition in calcium silicate based cement on
fibroblast cells viability. Methods: Chicken embryonic fibroblast cells were
immersed in extract solution of calcium silicate based cement containing steroid
and MTA. Viability was analyzed by MTT Assay. Results: Significant difference
(p ≤ 0,05) of viability on calcium silicate based cement containing steroid group
was found, compared to control and MTA group. Conclusion: Steroid addition
decrease viability. There was an increase in 72 hours, marking cells proliferation."
Fakultas Kedokteran Gigi Universitas Indonesia, 2015
SP-PDF
UI - Tugas Akhir  Universitas Indonesia Library
cover
Dewi Sri Kesumawati
Efek toksisitas odontocem dan MTA-Angelus terhadap viabilitas sel fibroblas = Effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability
"ABSTRAK
Latar Belakang: MTA bersifat biokompatibel dan dapat digunakan untuk perawatan kaping pulpa. Saat ini dikembangkan semen berbasis kalsium silikat sama seperti MTA dengan penambahan steroid, yaitu Odontocem. Tujuan:Membandingkan efek toksisitas odontocem dan MTA-Angelus terhadap viabilitas sel fibroblas. Metode:Sel fibroblast embrio ayam direndam dalam larutan odontocemdan dan MTA-Angeluspada 24 dan 72 jam. Viabilitas sel dihitung menggunakanMTT Assay. Hasil:Pada kelompok odontcemdan MTA-Angelus, terdapat perbedaan bermakna (p≤0,05 ) dibandingkan dengan kontrol. Pada paparan 24 jam, tidak terdapat perbedaan bermakna antara odontocem dengan MTA-Angelus. Kesimpulan:Odontocem dan dan MTA-Angelus menurunkan viabilitas sel pada 24 jam dan meningkatkan pada 72 jam.ABSTRACT
Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours."
Fakultas Kedokteran Gigi Universitas Indonesia, 2015
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Ayu Asri Lestari
Hubungan Antara Faktor Determinan Tooth Decay dan Filling di Indonesia (Pemetaan faktor determinan menggunakan Geographic Information System) = Relationship Between Determinant Factors Tooth Decay and Filling in Indonesia (Mapping Determinant Factors Using Geographic Information System)
"Latar Belakang: Salah satu tujuan dalam keikutsertaan Indonesia pada program pembangunan global berkelanjutan (SDGS) yaitu meningkatkan status kesehatan. Upaya tersebut tidak terlepas dari berbagai faktor pendukung seperti ketersediaan sumber daya tenaga medis, kemudahan akses manusia terhadap fasilitas kesehatan, sehingga manusia dengan mudah berkunjung dan mendapatkan perawatan di fasilitas kesehatan.
Tujuan : Mengetahui hubungan antara faktor sosiodemografi dan pelayanan kesehatan tooth decay dan filling di Indonesia. Metode: Penelitian cross sectional menggunakan data sekunder Riskesdas 2018 yang diklasifikasikan berdasarkan umur WHO sebanyak 14.031 mengenai variabel karakteristik sosidemografi (umur, jenis kelamin, tempat tinggal, status pekerjaan, dan tingkat pendidikan), utilisasi frekuensi kunjungan dan total decay serta filling. Pada Rifaskes 2019 menggunakan data sebanyak 17.741 fasilitas kesehatan. Jumlah dokter gigi menggunakan data Konsil Kedokteran Indonesia hingga bulan Desember 2021 sebanyak 22.926 dokter gigi. Variabel-variabel tersebut di uji secara statistik kemudian dipetakan menggunakan QGIS.
Hasil: Uji Mann-Whitney menunjukkan terdapat perbedaan bermakna (p<0,05) antara kelompok jenis kelamin, lokasi tempat tinggal dan pencarian pengobatan terhadap tooth decay dan filling. Uji Kruskal-Wallis menunjukkan terdapat perbedaan bermakna (p<0,05) antara kelompok status pekerjaan, tingkat pendidikan, dan utilisasi frekuensi kunjungan. Sedangkan Uji korelasi Spearman menunjukkan terdapat korelasi antara jumlah dan rasio fasilitas kesehatan serta dokter gigi terhadap tooth decay dan filling.
Kesimpulan: Pada penelitian ini terdapat hubungan antara faktor sosidemografi dan pelayanan kesehatan yang memengaruhi tooth decay dan filling. Upaya pemerataan distribusi fasilitas kesehatan dan dokter gigi, serta intervensi dalam meningkatkan utilisasi dengan melihat aspek karakteristik sosiodemografi.
Backgorund: One of the goals of Indonesia's participation on sustainable development goals is improving health state. Efforts made to achieve health improvement is increasing availability of health care facilities so people can easily access and get treatment for dental and oral health.
Objective: This study aimed to determine relationship between sociodemographic and health services factors that affect tooth decay and filling in Indonesia.
Methods: A cross sectional study using secondary data from Riskesdas 2018 as classified based WHO age as much 14.031 subject are sociodemography factors, utilization dental visit, total decay and filling. Rifaskes 2019 data’s using 17.741 healthcare facilities. Number of dentist as much 22.926 using data Indonesia Medical Council on December 2021. These variabels were tested statistically then mapping using QGIS.
Results: Mann-Whitney test showed a significant difference (p<0,05) between groups of gender, place of residence towards tooth decay and filling. Kruskal-Wallis test showed a significant difference (p<0,05) between groups of occupational status, education level, and frequency utilization towards tooth decay and filling. Spearman test showed a correlation statistically (p<0,05) between amount and ratio of healthcare facilities and dentist towards tooth decay and filling.
Conclusion: In this study there are relationship between sociodemographic factors and healthcare services that affect tooth decay and filling. Efforts to equitable distribution of healthcare facilities and dentist, as well as intervention to increase utilization by looking all aspect of sociodemographic characteristics."
Depok: Fakultas Kedokteran Gigi Universitas Indonesia, 2022
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Ujang Saeful Hikmat
Karakteristik Subset Monosit CD14,CD16 dan Ekspresi Mediator Inflamasi IL-1? dan IL-10 Pada Pasien DM Tipe 2 dan Hubungannya dengan Risiko Penyakit Kardiovaskular = Characteristic of CD14, CD16 monocyte subset and expression IL-1β, IL-10 inflammatory mediators in T2DM patients and its correlation with cardiovascular disease risks
"Aspek metabolik komplikasi DM tipe 2, khususnya penyakit kardiovaskular, telah banyak dibahas, namun aspek imunometabolik masih terbatas, sehingga sangat penting untuk memahami peran sistem imun dalam perkembangan komplikasi. Tujuan penelitian ini adalah untuk memahami peran subset monosit (CD14,CD16) dan mediator inflamasinya (IL-1β, IL-10) terhadap risiko penyakit kardiovaskular pada Pasien DM tipe 2. Subset monosit CD14, CD16 diperiksa menggunakan sampel kultur PBMC dan dianalisis menggunakan flow cytometry. Metode Multiplex Immunoassays digunakan untuk mengukur IL-1β, dan IL-10. Hasil penelitian ini, menunjukkan terdapat pola peningkatan subset monosit CD14+, CD16+ pada DM tipe 2, namun tidak berbeda secara signifikan. Peningkatan monosit CD14+,CD16+ lebih dari 6.8% berhubungan dengan peningkatan risiko penyakit kardiovaskular. Rasio mediator inflamasi IL-1β, sebelum dan sesudah stimulasi LPS secara signifikan lebih tinggi pada DM tipe 2 dibandingkan kontrol. Pada kondisi inflamasi, peningkatan IL-10 berespon terhadap stimulasi LPS, namun tidak mampu mengkompensasi peningkatan IL-1β, sehingga kecenderungan menjadi lebih hiperinflamasi pada DM tipe 2. Glukosa puasa merupakan penanda metabolik yang berhubungan dengan peningkatan monosit CD14+,CD16+.
The metabolic aspects of Type 2 Diabetes (T2D) complications, particularly cardiovascular disease, have been widely discussed, but the immunometabolic aspects are still limited, so it is critical to understand the role of the immune system in the development of complications. The objective of this study is to understand about the role of the monocyte subset (CD14,CD16) and its inflammatory mediators (IL-1β, IL-10) in the risk of CVD in T2D. CD14, CD16 monocyte subset was examined using PBMC culture samples and analyzed using flow cytometry. The Multiplex Immunoassays method was used to measure IL-1β and IL-10. This study shows there is an increase in the CD14+, CD16+ monocyte subset in type 2 diabetes, but it is not significantly related. An increase in CD14+,CD16+ monocytes of more than 6.8% is associated with an increased risk of CVD. The ratio of the inflammatory mediator IL-1β to basal conditions and LPS stimulation was significantly higher in T2D than in controls. In inflammatory conditions, the increase in IL-10 responds to LPS stimulation, but it is unable to compensate for the increase in IL-1β in T2D, so the tendency becomes more hyperinflammatory in type 2 DM. Fasting glucose is a metabolic marker associated with an increase in CD14+,CD16+ monocytes"
Jakarta: Fakultas Kedokteran Universitas Indonesia, 2023
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Dhani Rinaldi Ardiansyah
Analisis faktor pekerjaan, individu dan psikososial terhadap terjadinya gangguan otot tulang rangka akibat kerja pada pekerjaan perancah di PT. X = Analysis of occupational, individual and psychosocial factors on the occurrence of musculoskeletal disorders in scaffolding work at PT. X.
"Perancah merupakan bagian yang tidak dapat dipisahkan dari suatu pekerjaan konstruksi, Pekerjaan perancah berkontribusi pada munculnya faktor risiko gangguan otot tulang rangka akibat kerja (gotrak) atau musculoskeletal disorders (MSDs). Tujuan penelitian ini adalah menganalisis faktor risiko terjadinya gotrak pekerja perancah di PT X. Jenis penelitian adalah potong lintang dengan responden karyawan di PT X sebanyak 156 karyawan. Penilaian faktor risiko ergonomi di tempat kerja dilakukan dengan pendekatan penilaian tingkat risiko pekerjaan dan keluhan subjektif pekerja. Responden memberikan informasi karakteristik individu, risiko ergonomi menggunakan metode (Rapid Entire Body Assessment atau REBA) dengan hasil yang mencapai nilai tinggi dan sangat tinggi. Tingkat risiko ergonomi menunjukkan 66,23% responden termasuk kategori risiko tinggi dan 33,77 % responden termasuk kategori risiko rendah. Analisis keluhan gotrak pada pekerja menggunakan kuesioner Nordic Body Map menghasilkan 3 keluhan tertinggi yaitu leher bahu dan tangan/pergelangan. Penelitian ini menunjukkan bahwa faktor risiko terjadinya Gotrak pekerja perancah di PT X pada gotrak 12 bulan yaitu usia ≥ 30 tahun OR 1,91(95% CI 1,37-3,25), masa kerja ≥ 10 tahun OR 2,42(95% CI 1,39-4,19), Jenis Pekerjaan perancah OR 8,77() (95% CI 3,93-19,55) dan Skor REBA tinggi OR 2,81 (95% CI 1,39-5,67). Sedangkan faktor risiko gotrak yang menyebabkan absen 12 bulan terakhir adalah usia ≥ 30 tahun OR 1.32 (95% CI 1.18-1.76), masa kerja ≥ 10 tahun OR 1.65 (95% CI 1.03-2.65), jenis pekerjaan perancah OR 10,98(95% CI 4,26-28,26), skor REBA tinggi 2,53(1,78-3,00), demands at work tinggi OR 1.65 (95% CI 1.02-2.51), work organization and job contents tinggi OR1.44 (95% CI 1.28-2.93), untuk faktor risiko 7 hari terakhir yaitu jenis pekerjaan perancah OR 2,79(95% CI 1,28-6,07), health and wellbeing rendah OR 1.43 (95% CI 1.09-1.84).
Scaffolding is an inseparable part of a construction work. Scaffolding work contributes to the emergence of risk factors for skeletal muscle disorders due to work (gotrak) or musculoskeletal disorders (MSDs). The purpose of this study was to analyze the risk factors for the occurrence of scaffolding at PT X. This type of research was crosssectional with 156 employees as respondents at PT X. Ergonomics risk factor assessment in the workplace is carried out with an approach to assessing the level of occupational risk and subjective complaints of workers. Respondents provided information on individual characteristics, ergonomic risks using the method (Rapid Entire Body Assessment or REBA) with the results achieving high and very high scores. The level of ergonomics risk shows that 66.23% of respondents are in the high risk category and 33.77% of the respondents are in the low risk category. The analysis of cough complaints on workers using the Nordic Body Map questionnaire resulted in the 3 highest complaints, namely neck, shoulders and hands/wrist. This study shows that the risk factors for the occurrence of Gotrak scaffold workers at PT X at 12 months old are age 30 years OR 1.91 (95% CI 1.37-3.25), working period 10 years OR 2.42 (95 % CI 1.39-4.19), awkward posture OR 6.24 (95% CI 2.40-16.21). While the risk factors for gotrak that caused the absence of the last 12 months are age 30 years OR 1.32 (95% CI 1.18-1.76), years of service ≥ 10 years OR 1.65 (95% CI 1.03-2.65), type of work OR 10.98 (95% CI 4.26- 28.26), REBA score 2.53 (1.78-3.00), demands at work OR 1.65 (95% CI 1.02-2.51), work organization and job contents OR1.44 (95% CI 1.28-2.93, for risk factors for the last 7 days, namely type of work OR 2.79 (95% CI 1.28-6.07), health and wellbeingOR 1.43 (95% CI 1.09-1.84)."
Depok: Fakultas Kesehatan Masyarakat Universitas Indonesia, 2021
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