Hasil Pencarian  ::  Simpan CSV :: Kembali

"Profil hTERT dan P73 pada kultur sel kanker mulut dibandingkan mukosa normal: studi pendahuluan. Perubahan genetik pada p53 memungkinkan terjadinya imortalisasi sel dan meningkatkan predisposisi terjadinya transformasi ke arah neoplasma. Hal ini terkait dengan aktivitas telomerase yang menjaga panjang telomer, yang aktivitasnya seharusnya dibatasi oleh p53. p73, yang merupakan homolog p53, mempunyai kemampuan supresi tumor yang mirip dengan p53. Tingkat ekspresi P73 dan human TERT kemungkinan berbeda pada berbagai jenis sel kanker dibandingkan dengan jaringan normalnya. Saat ini belum terdapat data tentang profil protein human TERT dan P73 pada sel lini karsinoma sel skuamosa oral (KSSO) dibandingkan dengan jaringan mukosa oral normal. Tujuan: Studi ini bertujuan untuk mendapatkan profil human TERT dan P73 pada sel lini KSSO dengan mutasi p53 dibandingkan dengan mukosa oral normal. Metode: Sel lini HSC-3 dan HSC-4 dengan mutasi p53 dikultur sedangkan mukosa oral normal dikumpulkan dari pasien tanpa kanker yang menjalani perawatan bedah mulut. Ekstraksi protein dan analisis profil human TERT dan P73 dilakukan dengan SDS-PAGE dan ditentukan berdasarkan perkiraan berat protein. Hasil: Pita human TERT jelas terlihat pada sel HSC-3 dan HSC-4 namun tidak pada mukosa oral normal. Densitas pita P73 pada sel HSC-3 terlihat lebih tebal dibandingkan pada sel HSC-4. Hanya 50% sampel mukosa oral normal memperlihatkan pita P73. Simpulan: Terlihat profil human TERT dan P73 yang berbeda pada sel kanker dan sel normal. Perbedaan ini kemungkinan bersifat spesifik tergantung jenis sel dan dipengaruhi oleh status p53. Perlu dilakukan penelitian lanjutan untuk menganalisis kemungkinan peran p73 dalam menggantikan fungsi p53 pada kanker mulut selanjutnya diperlukan.

---

Genetic alteration on p53 allows cellular immortalization and predisposes cells to neoplastic transformation. This immortalization is related to telomere length maintenance by telomerase. Human TERT is a key component of telomerase, which activity is suppressed by p53. The p73, the homolog of p53, has a similar ability in tumor suppression. The P73 is expressed at a different level in various cancer cells and normal tissues. Profile of human TERT and P73 in mutant p53 OSCC cell line and normal human oral mucosa have not been known.Objective: To observe human TERT and P73 profile in mutant p53 OSCC cell lines and normal human oral mucosa. Methods: The extracted protein of HSC-3 and HSC-4 cell lines and normal mucosal tissues were analyzed with SDS PAGE to detect human TERT and p73 expression based on the molecular weight. Results: The HSC-3 cell line showed thicker band density of P73 compared to its density of HSC-4. Only 50% of normal oral mucosa tissue showed thick P73 band density. The human TERT band was clearly shown in HSC-3 and HSC-4 cell lines but not in normal oral mucosa. Conclusion: Different profile of human TERT and P73 in OSCC cell lines and normal oral mucosa might be cell-type specific and influenced by the status of p53. Analysis of the role of p73 in these cancers might need further research to determine possible p73 substitution for p53 function."

"Latar Belakang: p73, homolog p53, diketahui memiliki kemampuan serupa dalam menekan pertumbuhan tumor. Protein p73 diekspresikan dalam berbagai level pada sel kanker dan jaringan normal yang berbeda. Belum diketahui bagaimana pola ekspresi protein p73 pada KSSRM dan pada jaringan mukosa mulut normal.Tujuan: Mengetahui profil protein p73 pada KSSRM tipe mutant p53 dan jaringan mukosa mulut normal berdasarkan berat molekul protein.Metode: Ekstrak protein dari HSC-3 dan HSC-4 serta jaringan mukosa normal dianalisa dengan teknik SDS PAGE untuk mendeteksi protein p73 berdasarkanberat molekulnya.Hasil:. pita protein p73 pada HSC-3 lebih tebal daripada HSC- 4. Terdapat variasi profil protein p73 pada mukosa mulut normal dengan pita protein tebal (8/17) dan sedang (5/17).Simpulan: Terdapat perbedaan profil protein p73 antara HSC-3 dan HSC-4 berkaitan dengan tingkat protein p53 dan SNP pada kodon 72. Kebanyakan sampel jaringan mukosa memperlihatkan ketebalan pita protein p73 yang cukup tinggi.

---

Backround: p73, the homolog of p53, has a similar ability in tumor suppression. p73 protein expressed at a different level in various cancer cells and normal tissues. Profile of p73 protein in mutant p53 OSCC cell line and normal human oral mucosa have not been known.

Objectives: To observe p73 protein profile in mutant p53 OSCC cell lines and normal human oral mucosa.

Methods: The extracted protein of HSC-3 and HSC-4 cell lines and normal mucosal tissues were analyzed with SDS PAGE to detect p73 protein based on the molecular weight.

Results:. The band of p73 protein in HSC-3 shows a higher density compared to its density of HSC-4. A thick p73 protein band was shown on 8/17 of normal mucosal tissues while medium level of p73 protein band was shown on 5/17.

Conclusion: The protein profile between HSC-3 and HSC-4 were different related with p53 protein and SNP on codon 72 of each samples. Most of mucosal tissues shows a quite high density of p73 protein bands."

"Protein sel merupakan makromolekul yang terdiri dari satu atau beberapa polipeptida yang tersusun dari rangkaian asam amino yang saling berikatan. Terdapat perbedaan profil protein antara sel normal dengan sel kanker.Tujuan : Melihat ekspresi protein pada KSSRM dan mukosa mulut normal.Metode : Sel galur HSC-3 dan HSC-4 dikultur hingga confluent. Sel skuamosa mukosa normal diambil dari jaringan gingiva pasien odontektomi. Semua sampel dilakukan prosedur ekstraksi protein, Bradford protein assay, dan SDS PAGE.Hasil : KSSRM mengekspresikan protein dengan level cukup tinggi pada berat molekul 31-78 kDa. Namun, pada mukosa normal, kebanyakan mengekspresikan protein pada berat molekul antara 39 - 172 kDa.Kesimpulan : Terdapat perbedaan ekspresi protein pada sel galur KSSRM dibandingkan dengan mukosa mulut normal.

---

Cells Proteins are macro molecules consist of one or several polypeptides which formed from amine acid chain that bound one another. There is a different of protein profile between normal and cancer cells.Objective : To observe the protein expression in OSCC and normal oral mucous.Method : Cell lines HSC-3 and HSC-4 were cultured until confluent. Normal squamous mucosa was taken from gingival tissues patient who had odontectomy procedure. Protein extraction, Bradford protein assay, and SDS PAGE procedure were performed for all samples.Results : Oral squamous cells carcinoma expressed rather high level of protein which have molecular weight of 31-78 kDa compared to normal gingival which express protein molecular weight ranging between 39 - 172 kDa.Conclusion : There are different protein expression between oral squamous cells carcinoma and normal oral mucous."

"Indonesian Journal of Dentistry 2006; Edisi Khusus KPPIKG XIV:239-242Oral squamous cell carcinorna ( OSCC ) is the most common malignant tumor of the oral cavity, and its account for 80-90% of all malignancies in oral cavity. The aim of this study was to detemine the presenceof p53 mutations and to associate these mutations with the histopathological type of OSCC such as well differentiated and poorly differentiated. Analitycal observational comparative study by cross sectional design was used. Forty untreated well and poorly differentiated OSCC biopsy sample and normal tissue biopsy material taken from 16 normal patients were analyzed for the presence of mutation in the conserved region of the p53 gene especially in exon 5 by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The results of this study showed that p53 gene mutations were detected in exon 5; 11/40 (27,5%) with heterozygous mutation 9/11 (81,8%). The incidence in exon 5 of p53 gene mutation was significantly accociated with well differentiated 2/20 (l0%) and poorly diferentiated 9/20 (45%) OSCC(P=0,013). This study concludes that 1) mutation in exon 5 of p53 gene occured frequently in OSCC; 2) exon 5 of the p53 gene could be one of the the specific targets for histopathological grade of OSCC; 3) mutation in exon 5 of p53 gene could be important prognostic factor in OSCC."

"Latar Belakang: Kanker mulut, khususnya karsinoma sel skuamosa rongga mulut (KSSRM), sering ditemukan di Asia, termasuk Indonesia, terutama pada pria usia lanjut dengan lokasi utama di lidah. Prognosis pasien sangat bergantung pada deteksi dini. Biopsi dan pemeriksaan histopatologis menggunakan pewarnaan hematoksilin dan eosin (H&E) merupakan metode utama, salah satunya penilaian derajat diferensiasi jaringan. Namun, diperlukan tambahan parameter seperti derajat keratinisasi dan reaksi desmoplastik untuk penilaian yang lebih objektif. Selain itu juga, dapat melihat perubahan jaringan dengan melihat daerah yang bersifat displastik. Tujuan: Menentukan derajat diferensiasi jaringan KSSRM berdasarkan derajat keratinisasi dan klasifikasi reaksi desmoplastik serta pengamatan perubahan sifat jaringan. Metode: Penelitian deskriptif analitik menggunakan sampel jaringan KSSRM yang diwarnai H&E dan diamati menggunakan mikroskop cahaya. Hasil: Belum ditemukan hubungan bermakna (p>0,05) antara usia, jenis kelamin, lokasi tumor, derajat keratinisasi, dan klasifikasi reaksi desmoplastik terhadap derajat diferensiasi. Perubahan jaringan displastik menjadi cancerous dapat diamati pada beberapa sampel. Kesimpulan: Usia, jenis kelamin, lokasi tumor, derajat keratinisasi, dan klasifikasi reaksi desmoplastik belum menunjukkan hubungan bermakna dengan derajat diferensiasi KSSRM. Perubahan jaringan yang bersifat displastik menjadi cancerous dapat diamati pada beberapa jaringan KSSRM dengan masih terlihatnya daerah displastik.

---

Background: Oral cancer, particularly oral squamous cell carcinoma (OSCC), is commonly found in Asia, including Indonesia, especially in older males with the primary site being the tongue. Patient prognosis heavily depends on early detection. Biopsy and histopathological examination using hematoxylin and eosin (H&E) staining remain the main diagnostic methods, including the assessment of tissue differentiation grade. However, additional parameters, such as the degree of keratinization and desmoplastic reaction, are needed for more objective evaluation. In addition, tissue changes can be seen by looking at dysplastic areas. Objective: To determine the differentiation grade of OSCC tissue based on the degree of keratinization and desmoplastic reaction and observation of changes in tissue properties. Methods: A descriptive-analytical study using OSCC tissue samples stained with H&E and observed under a light microscope. Results: No significant relationship (p>0.05) was found between age, gender, tumor location, degree of keratinization, and desmoplastic reaction with the differentiation grade. Dysplastic-to-cancerous transformation was observed in some samples. Conclusion: Age, gender, tumor location, degree of keratinization, and desmoplastic reaction did not show a significant relationship with the differentiation grade of OSCC. Changes in tissue from dysplastic to cancerous in several OSCC tissue were still seen."

"Latar Belakang: Kanker mulut, khususnya karsinoma sel skuamosa rongga mulut (KSSRM), sering ditemukan di Asia, termasuk Indonesia, terutama pada pria usia lanjut dengan lokasi utama di lidah. Prognosis pasien sangat bergantung pada deteksi dini. Biopsi dan pemeriksaan histopatologis menggunakan pewarnaan hematoksilin dan eosin (H&E) merupakan metode utama, salah satunya penilaian derajat diferensiasi jaringan. Namun, diperlukan tambahan parameter seperti derajat keratinisasi dan reaksi desmoplastik untuk penilaian yang lebih objektif. Selain itu juga, dapat melihat perubahan jaringan dengan melihat daerah yang bersifat displastik. Tujuan: Menentukan derajat diferensiasi jaringan KSSRM berdasarkan derajat keratinisasi dan klasifikasi reaksi desmoplastik serta pengamatan perubahan sifat jaringan. Metode: Penelitian deskriptif analitik menggunakan sampel jaringan KSSRM yang diwarnai H&E dan diamati menggunakan mikroskop cahaya. Hasil: Belum ditemukan hubungan bermakna (p>0,05) antara usia, jenis kelamin, lokasi tumor, derajat keratinisasi, dan klasifikasi reaksi desmoplastik terhadap derajat diferensiasi. Perubahan jaringan displastik menjadi cancerous dapat diamati pada beberapa sampel. Kesimpulan: Usia, jenis kelamin, lokasi tumor, derajat keratinisasi, dan klasifikasi reaksi desmoplastik belum menunjukkan hubungan bermakna dengan derajat diferensiasi KSSRM. Perubahan jaringan yang bersifat displastik menjadi cancerous dapat diamati pada beberapa jaringan KSSRM dengan masih terlihatnya daerah displastik.

---

Background: Oral cancer, particularly oral squamous cell carcinoma (OSCC), is commonly found in Asia, including Indonesia, especially in older males with the primary site being the tongue. Patient prognosis heavily depends on early detection. Biopsy and histopathological examination using hematoxylin and eosin (H&E) staining remain the main diagnostic methods, including the assessment of tissue differentiation grade. However, additional parameters, such as the degree of keratinization and desmoplastic reaction, are needed for more objective evaluation. In addition, tissue changes can be seen by looking at dysplastic areas. Objective: To determine the differentiation grade of OSCC tissue based on the degree of keratinization and desmoplastic reaction and observation of changes in tissue properties. Methods: A descriptive-analytical study using OSCC tissue samples stained with H&E and observed under a light microscope. Results: No significant relationship (p>0.05) was found between age, gender, tumor location, degree of keratinization, and desmoplastic reaction with the differentiation grade. Dysplastic-to-cancerous transformation was observed in some samples. Conclusion: Age, gender, tumor location, degree of keratinization, and desmoplastic reaction did not show a significant relationship with the differentiation grade of OSCC. Changes in tissue from dysplastic to cancerous in several OSCC tissue were still seen."

"Pendahuluan: Insidensi Infeksi HPV pada Karsinoma Sel Skuamosa Oral dan Hubungannya dengan Mutasi p53 dan c-myc: Penelitian Kasus Kontrol pada Rumah Sakit Muwardi. Rata-rata angka kejadian kanker rongga mulut dan pharyng di negara berkembang per tahun diperkirakan pada kisaran 25 kasus per 100.000 penduduk. Saatini patogenesis kanker telah diketahui berhubungan dengan virus Human papilloma (HPV). Catatan lain bahwa mutasi pada gena p53 dan c-myc ditemukan pada 50% dari seluruh kejadian kanker. Tujuan: Tujuan penelitian ini adalah untuk mengetahui kejadian pasien Kanker Sel Skuamous Rongga Mulut (KSSRM) dengan infeksi HPV tanpa mutasigena p53 dan c-myc. Bahan dan Cara: Jaringan biopsi frozen sections dari pasien Sel Skuamous Rongga Mulut Jinak (SSRMJ) dan Kanker Sel Skuamous Rongga Mulut (KSSRM) yang dikumpulkan dari Bagian Penyakit Gigi dan Mulut RSUD dr Muwardi Surakarta mulai Januari 2007 hingga Januari 2008. Amplifikasi gena L1-HPV untuk mengetahuikeberadaan stressor HPV. Amplifikasi gena-gena p53 dan c-myc, dilanjutkan analisis Single Strand Comformational Polymorphisme (SSCP) dan diikuti pengukuran menggunakan densitometer untuk melihat keberadaan mutasi. Data yang terkumpul dianalisa menggunakan Uji Chi Square. Hasil: Pasien SSRMJ teridentifikasi 23% terinfeksi HPV dan pasien KSSRM teridentifikasi 73% terinfeksi HPV. Seratus persen pasien SSRMJ yang terinfeksi HPV tercatat tanpa mutasi pada gena p53 dan c-myc, 81% pasien KSSRM yang terinfeksi HPV tercatat tanpa mutasi gena p53 dan 91 % pasien KSSRM yang terinfeksi HPV tercatat tanpa mutasi gena c-myc. Analisis uji Chi Square menunjukkan perbedaan yang signifikan antara pasien SSRMJ dan KSSRM pada infeksi HPV tanpa mutasi gena p53 dan c-myc. Kesimpulan: HPV merupakan faktor untuk kejadian KSSRM.

---

Introduction: Annual incidence rates for oral and pharyngeal cancer are estimated at 25 cases per 100,000 in developing countries. Human papilloma virus (HPV) was implicated in pathogenesis of Cancer. The mutations of p53 and c-myc are found 50% in cancer. Objective: Aims of this research were to know the incidence of OSSC patient which realized HPV infection without p53 and c-myc gene mutation. Materials and Methods: Tissue biopsy frozen sections were taken from BOSC (Benign Oral Squamous Cell) and OSCC (Oral Squamous Cell Carcinoma) patients collected from Oral and Dental Departement of dr Muwardi Distric Hospital in Surakarta from January 2007 to January 2008. Toamplify L1-HPV gene for fixed the HPV stressor. To amplify p53 and c-myc genes, continued with SSCP (Single Strand Conformational Polymorphisme) analysis and followed with measurement using densitometer, to see mutation existence. The collected data were analyzed with Chi Square. Results: BOSC patient identified 23% with HPV infections and OSCC patient identified 73% with HPV infections. Hundred percent BOSC patient with HPV infection without mutation in p53 gene and c-myc gene, 81% OSCC patient with HPV infection without mutation in p53 gene and 91 % OSCC patient with HPV infection without mutation in c-myc gene. Chi Square analysis showed significant difference between BOSC and OSCC patients with HPV infection without mutation in p53 and c-myc gene. Conclusion: HPV is a factor for pathogenesis of OSCC."

"belakang: Karsinoma sel skuamosa rongga mulut (KSSRM) adalah salah satu kanker yang paling umum dijumpai dengan angka survival 52.0% yang tidak meningkat secara bermakna walaupun tatalaksana kanker ini terus berkembang. Cornulin merupakan protein spesifik untuk sel skuamosa yang penting dalam diferensiasi epitel. Penelitian sebelumnya menunjukkan bahwa rendahnya ekspresi cornulin berhubungan dengan gambaran klinikopatologi dan survival yang lebih buruk dibandingkan dengan ekspresi tinggi. Oleh karena sifatnya yang spesifik dan belum ada penelitian mengenai ekspresi cornulin sebagai faktor prognosis di Indonesia, maka penulis tertarik untuk mengetahui hubungan antara ekspresi cornulin dan survival pada pasien dengan KSSRM.Tujuan: Mengetahui potensi cornulin sebagai penanda biologis survival pada pasien dengan KSSRM.Metode: Penelitian ini menggunakan desain studi kohort retrospektif yang dilakukan dari periode Juni 2021 sampai dengan Mei 2022. Populasi penelitian ini merupakan pasien dengan diagnosis KSSRM yang ditegakkan dengan pemeriksaan histopatologis dan menjalani terapi di Divisi Bedah Onkologi Departemen Ilmu Bedah RSCM periode Januari 2015 – Mei 2020. Pemeriksaan imunohistokimia dilakukan untuk mengetahui ekspresi cornulin dan skor imunihistokimia ditentukan menggunakan immunoreactive score (IRS). Skor IRS < 6 berarti ekspresi rendah dan ≥ 6 berarti ekspresi tinggi. Analisis statistik univariat, bivariat, dan survival dilakukan menggunakan perangkat lunak SPSS.Hasil: Cornulin tidak memiliki hubungan yang signifikan secara statistik dengan survival pada pasien dengan KSSRM. T, N, dan stadium memiliki hubungan yang signifikan secara statistik dengan survival pada pasien dengan KSSRM dengan nilai p masing-masing adalah 0.001, 0.040, dan 0.001. T dan N memiliki hubungan yang bermakna secara statistik dengan ekspresi cornulin pada pasien dengan KSSRM, dengan nilai p masing-masing adalah 0.034 dan 0.030.Kesimpulan:Cornulin sebagai protein penanda biologis KSSM tidak dapat menjadi prediktor dari survival pasien dengan KSSM.

---

Background: Oral squamous cell carcinoma (OSCC) is one of the most common cancers with a 52.0% survival rate which does not increase significantly even though the management of this cancer continues to develop. Cornulin is a specific protein for squamous cells that is important in epithelial differentiation. Previous studies have shown that low cornulin expression is associated with worse clinicopathological features and survival compared to high cornulin expression. Due to its specific nature and no research on cornulin expression as a prognostic factor has been done in Indonesia, the author is interested in knowing the relationship between cornulin expression and survival in patients with OSCC.

Objective: To determine the potential of cornulin as a biological marker for survival in patients with OSCC.

Methods: This study used a retrospective cohort study design that was conducted from June 2021 to May 2022. The population of this study were patients with OSCC diagnosis confirmed by histopathological examination and undergoing therapy at the Division of Surgical Oncology, Department of Surgery, RSCM for the period January 2015-May 2020. Immunohistochemical examination was performed to determine the expression of cornulin and the immunohistochemical score was calculated using the immunoreactive score (IRS). IRS score < 6 means low cornulin expression and ≥ 6 means high cornulin expression. Univariate, bivariate, and survival statistical analyses were performed using SPSS software.

Results: Cornulin did not have a statistically significant relationship with survival in patients with OSCC. T, N, and stage had a statistically significant relationship with survival in patients with SCC with p values ​​of 0.001, 0.040, and 0.001, respectively. T and N had a statistically significant relationship with cornulin expression in patients with OSCC, with p-values ​​of 0.034 and 0.030, respectively.

Conclusion: Cornulin as a biological marker protein of OSCC cannot be a predictor of the survival of patients with OSCC."

"Indonesian Journal of Dentistry 2006; Edisi Khusus KPPIKG XIV: 353-357Oral squamous cell carcinoma represents more than 90% of all oral cancers. Ten percent of the cases are found on the buccal mucosa and the gingiva. The World Health Organization defines a premalignant or precancerous lesion as a morphologically altered tissue in which cancer is more likely occur and includes oral leukoplakia, oral erythroplakia, and possibly oral lichen planus (OLP). The purpose of this study was to discuss the possibility of malignant transformation of OLP. The potency of OLP as a premalignant lesion is still an ongoing controversial discussion in the literature. The report a case of oral squamous cell carcinoma located on the left buccal mucosa accompanied by oral lichenoid lesions on the right buccal mucosa, lower labial mucosa, and left buccal mucosa. These findings led to a possibility of malignant transformation of the oral lichenoid lesions. Unfortunately, biopsy on the lichenoid lesions was not performed. Therefore, a definitive diagnosis of OLP could be established and the possibility of the lesions being dysplastic remained unclear. This study concluded that biopsy is mandatory to establish a definitive diagnosis of OLP and to investigate the possibility of dysplasia. It is necessary to perform examination of genetic alterations in dysplastic OLP in order to assess loss of heterozygosity (LOH), which may help to consider the risk of malignant transformation."

"Latar belakang Kanker rongga mulut dan mulut (termasuk karsinoma sel skuamosa rongga mulut/KSSRM) secara kolektif tetap menjadi kanker paling umum ke-16 di dunia. Karena kecenderungan stadium lanjut selama diagnosis, kelangsungan hidup pasien KSSRM sangat buruk. Tumor infiltrated lymphocytes (TILs) yang diekspresikan diperkirakan mempengaruhi kelangsungan hidup pasien KSSRM, termasuk CD8 + dan TIL lainnya. Tujuan Untuk menentukan ekspresi CD8+ dan TILs dalam sel KSSRM dan hubungannya dengan overall survival (OS) dan progression-free survival (PFS) pasien KSSRM. Metode Penelitian ini merupakan analisis kelangsungan hidup dengan menggunakan desain kohort retrospektif pada pasien KSSRM yang datang ke Rumah Sakit Umum Nasional Cipto Mangunkusumo, Indonesia, dari Januari 2017 hingga Desember 2021. Kriteria inklusi penelitian adalah pasien KSSRM dengan diagnosis histopatologi, sedangkan kriteria eksklusi adalah pasien dengan data yang tidak lengkap atau tidak tersedianya sampel. Ekspresi CD8+ dan TIL diukur melalui perhitungan manual pada program Image J® pada pewarnaan imunohistokimia. OS dan PFS dianalisis menggunakan grafik Kaplan-Meier dan analisis cox-regression. Hasil Sebanyak 42 subjek dilibatkan dalam penelitian ini. Rata-rata OS adalah 10,83+1,268 bulan, sedangkan rata-rata PFS adalah 9,74+1,229 bulan. OS 2 tahun adalah 21,4%, sedangkan PFS adalah 19%. Ekspresi CD8+ yang lebih tinggi terkait dengan OS dan PFS yang lebih baik, sedangkan ekspresi TIL yang lebih tinggi terkait dengan PFS yang lebih baik. Kesimpulan. Ekspresi CD8+ dan TIL yang lebih tinggi dalam sel kanker terkait dengan kesintasan yang lebih baik pada pasien KSSRM.

---

Background Oral cavity and mouth cancer (including oral cavity squamous cell carcinoma (OCSCC) collectively remain the 16th most prevalent cancer in the world. Due to the tendency of advanced stage during diagnosis, the survival of OCSCC patients is abysmal. The connection of OCSCC and expressed tumor infiltrated lymphocytes (TILs) is thought to affect the survivability of the OCSCC patients, including CD8+ and other TILs. Aim To determine the expression of CD8+ and TILs in OCSCC cells and their relationship with overall survival (OS) and progression-free survival (PFS) of OCSCC patients. Methods This study is a survival analysis using retrospective cohort design on OCSCC patients who came to Cipto Mangunkusumo National General Hospital, Indonesia, from January 2017 to December 2021. The inclusion criterion of the study was OCSCC patients with histopathological diagnosis, while the exclusion criteria were patients with incomplete data or unavailability of the samples. The expression of CD8+ and TILs were measured by manual counting of cells using ImageJ® on immunohistochemistry staining. The OS and PFS were analyzed using Kaplan-Meier graph and cox-regression analysis. Result A total of 42 subjects were included in this study. The average OS was 10.83+1.268 months, while the average PFS was 9.74+1.229 months. The 2-years OS was 21.4%, while PFS was 19%. Higher CD8+ expression was related to better OS and PFS, while higher expressed TILs was related to better PFS. Conclusion Higher CD8+ and TILs expressions in cancer cells are related to better survivability in OCSCC patients. "