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"ABSTRAK Pendahuluan: Meningkatnya jumlah pasien dewasa dengan restorasi sewarna gigi, seperti resin komposit, menyebabkan perekatan braket pada permukaan artifisial gigi menjadi suatu tantangan tersendiri karena sering terjadi kegagalan rekat. Saat ini, belum ada riset yang dilakukan untuk melihat perbedaan nilai kekuatan rekat braket (baik nilai kuat geser maupun nilai kuat tarik braket) antara pada permukaan email gigi dan resin komposit nanohibrid. Metode: 32 gigi premolar bawah dibagi menjadi 4 kelompok: kelompok A1) spesimen email gigi (uji kuat rekat geser); kelompok A2) spesimen resin komposit nanohibrid (uji kuat rekat geser); kelompok B1) spesimen email gigi (uji kuat rekat tarik); kelompok B2) spesimen resin komposit nanohibrid (uji kuat rekat tarik). Braket direkatkan pada spesimen lalu diuji dengan menggunakan Universal Testing Machine Shimazu AG-5000 dalam waktu 24 jam setelah braket direkatkan. Hasil: Rerata nilai kuat rekat geser pada kelompok A1 sebesar 10.78 ± 0.13 MPa dan pada kelompok A2 sebesar 10.63 ± 0.18 MPa. Rerata nilai kuat rekat tarik pada kelompok B1 sebesar 10.74 ± 0.15 MPa dan pada kelompok B2 sebesar 10.65 ± 0.14 MPa. Pada uji statistik tidak terdapat perbedaan bermakna secara pada nilai kuat rekat geser maupun nilai kuat rekat tarik braket metal antara pada permukaan email gigi dan resin komposit nanohibrid. Kesimpulan: Tidak terdapat perbedaan bermakna secara statistik pada nilai kuat rekat geser maupun nilai kuat rekat tarik braket metal antara pada permukaan email gigi dan resin komposit nanohibrid. Seluruh kelompok memiliki nilai rerata kuat rekat geser dan nilai rerata kuat rekat tarik yang memadai untuk keperluan klinis perawatan ortodontik.

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ABSTRACT

Introduction: The increasing number of adult patients with tooth-colored restorations, such as composite resins, causes the attachment of brackets on artificial surfaces of teeth to be a challenge because of frequent adhesive failures. At present, no research has been carried out to see the difference in bracket adhesive strength values (both shear bond strength and tensile bond strength) between the enamel surface and nanohybrid composite resins surface. Methods: 32 lower premolar were divided into 4 groups: group A1) dental enamel specimens for shear bond strength test; group A2) nanohybrid composite resins specimens for shear bond strength test; group B1) dental enamel specimens for tensile bond strength test; group B2) nanohybrid composite resins specimens for tensile bond strength test. The bracket was bonded to the specimens and tested using Universal Testing Machine Shimazu AG-5000 within 24 hours after the bracket was bonded to the specimens. Results: The mean value of shear bond strength in group A1 was 10.78 ± 0.13 MPa and in group A2 was 10.63 ± 0.18 MPa. The mean value of tensile bond strength in group B1 was 10.74 ± 0.15 MPa and in group B2 was 10.65 ± 0.14 MPa. In the statistical test there was no significant difference in the shear bond strength value or the tensile bond strength value between the metal bracket bonded to the enamel surfaces and to the nanohybrid composite resin surfaces. Conclusion: There was no significant difference in the shear bond strength and the tensile bonding strength value of the metal bracket bonded to the enamel surface and the nanohybrid composite resin surfaces. All groups have an adequate bond strength value for the clinical needs of orthodontic treatment."

"Latar Belakang: Salah satu tujuan dalam keikutsertaan Indonesia pada program pembangunan global berkelanjutan (SDGS) yaitu meningkatkan status kesehatan. Upaya tersebut tidak terlepas dari berbagai faktor pendukung seperti ketersediaan sumber daya tenaga medis, kemudahan akses manusia terhadap fasilitas kesehatan, sehingga manusia dengan mudah berkunjung dan mendapatkan perawatan di fasilitas kesehatan.Tujuan : Mengetahui hubungan antara faktor sosiodemografi dan pelayanan kesehatan tooth decay dan filling di Indonesia. Metode: Penelitian cross sectional menggunakan data sekunder Riskesdas 2018 yang diklasifikasikan berdasarkan umur WHO sebanyak 14.031 mengenai variabel karakteristik sosidemografi (umur, jenis kelamin, tempat tinggal, status pekerjaan, dan tingkat pendidikan), utilisasi frekuensi kunjungan dan total decay serta filling. Pada Rifaskes 2019 menggunakan data sebanyak 17.741 fasilitas kesehatan. Jumlah dokter gigi menggunakan data Konsil Kedokteran Indonesia hingga bulan Desember 2021 sebanyak 22.926 dokter gigi. Variabel-variabel tersebut di uji secara statistik kemudian dipetakan menggunakan QGIS.Hasil: Uji Mann-Whitney menunjukkan terdapat perbedaan bermakna (p<0,05) antara kelompok jenis kelamin, lokasi tempat tinggal dan pencarian pengobatan terhadap tooth decay dan filling. Uji Kruskal-Wallis menunjukkan terdapat perbedaan bermakna (p<0,05) antara kelompok status pekerjaan, tingkat pendidikan, dan utilisasi frekuensi kunjungan. Sedangkan Uji korelasi Spearman menunjukkan terdapat korelasi antara jumlah dan rasio fasilitas kesehatan serta dokter gigi terhadap tooth decay dan filling.Kesimpulan: Pada penelitian ini terdapat hubungan antara faktor sosidemografi dan pelayanan kesehatan yang memengaruhi tooth decay dan filling. Upaya pemerataan distribusi fasilitas kesehatan dan dokter gigi, serta intervensi dalam meningkatkan utilisasi dengan melihat aspek karakteristik sosiodemografi.......Backgorund: One of the goals of Indonesia's participation on sustainable development goals is improving health state. Efforts made to achieve health improvement is increasing availability of health care facilities so people can easily access and get treatment for dental and oral health.Objective: This study aimed to determine relationship between sociodemographic and health services factors that affect tooth decay and filling in Indonesia.Methods: A cross sectional study using secondary data from Riskesdas 2018 as classified based WHO age as much 14.031 subject are sociodemography factors, utilization dental visit, total decay and filling. Rifaskes 2019 data’s using 17.741 healthcare facilities. Number of dentist as much 22.926 using data Indonesia Medical Council on December 2021. These variabels were tested statistically then mapping using QGIS.Results: Mann-Whitney test showed a significant difference (p<0,05) between groups of gender, place of residence towards tooth decay and filling. Kruskal-Wallis test showed a significant difference (p<0,05) between groups of occupational status, education level, and frequency utilization towards tooth decay and filling. Spearman test showed a correlation statistically (p<0,05) between amount and ratio of healthcare facilities and dentist towards tooth decay and filling. Conclusion: In this study there are relationship between sociodemographic factors and healthcare services that affect tooth decay and filling. Efforts to equitable distribution of healthcare facilities and dentist, as well as intervention to increase utilization by looking all aspect of sociodemographic characteristics."

"Latar Belakang: Setiap tahap odontogenesis yang tidak berjalan dengan baik semestinya dapat mengakibatkan terjadinya abnormalitas pada gigi, hal tersebut dipengaruhi oleh beberapa faktor atau komponen tertentu seperti gen, nutrisi, mineral, molekul, atau lainnya. Untuk memahami proses terjadinya abnormalitas serta faktor-faktor yang memengaruhinya, perlu diketahui secara spesifik setiap tahapan perkembangan gigi. Penelitian terkait perkembangan gigi pada manusia tidak memungkinkan karena diperlukan tindakan yang invasif dalam pengambilan sampel jaringan. Oleh sebab itu, digunakan hewan coba mencit C57BL/6 karena merupakan jenis inbred dan banyak digunakan dalam penelitian biomolekuler. Namun, hingga saat ini penelitian mengenai odontogenesis pada mencit C57BL/6 masih sangat terbatas. Tujuan: Menganalisis perkembangan gigi (odontogenesis) mencit strain C57BL/6 usia satu hari. Metode: Rahang mencit C57BL/6 dipotong menjadi 4 bagian. Setelah itu, dilakukan pembuatan preparat dengan potongan longitudinal (sagital) pada maksila dan mandibula kanan dan potongan koronal (frontal) pada maksila dan mandibula kiri, serta dilakukan pewarnaan hematoksilin dan eosin (H&E) pada preparat dan dilakukan pengamatan benih gigi insisif dan molar rahang atas dan rahang bawah menggunakan mikroskop. Hasil: Terlihat benih gigi insisif, molar pertama, molar kedua, dan molar ketiga pada jaringan maksila dengan potongan transversal atau axial dan terlihat adanya benih gigi molar pada preparat jaringan maksila dan mandibula dengan potongan koronal. Pada jaringan mandibula dengan potongan longitudinal atau sagital hanya terlihat adanya benih gigi insisif. Kesimpulan: Terjadi proses odontogenesis hingga tahap awal bell stage pada gigi molar maksila kiri dan mandibula kiri mencit C57BL/6 usia satu hari, sehingga mencit C57BL/6 usia satu hari dapat dijadikan alternatif objek penelitian dalam menganalisis perkembangan struktur jaringan gigi baik pada kondisi normal maupun patologis.......Background: Disruption in the stages of odontogenesis results in teeth abnormalities. These conditions can be influenced by certain factors or components such as genes, nutrients, minerals, molecules, or others. To understand the process of abnormality and the factors that influence it, it is necessary to know specifically each stage of tooth development. Research related to the development of teeth in humans is not possible because it requires an invasive procedure in tissue sampling. Therefore, C57BL/6 mice were used as experimental animals because they are an inbred species and are widely used in biomolecular research. However, until now research on odontogenesis in C57BL/6 mice is still very limited. Objective: Analyzing tooth development (odontogenesis) in one-day-old C57BL/6 mice. Methods: The jaws of C57BL/6 mice were cut into 4 parts. After that, preparations were made with longitudinal (sagittal) sections on the right maxilla and mandible and coronal (frontal) sections on the left maxilla and mandible, and stained with hematoxylin and eosin (H&E) on the preparations and observation of the maxillary and mandibular incisors and molars using a microscope. Results: Tooth germs of incisors, first molars, second molars, and third molars were observed in the maxillary tissue with transverse or axial sections and the presence of molars in the maxillary and mandibular tissue preparations with coronal sections. In mandibular tissue with longitudinal or sagittal sections, only incisor germs were seen. Conclusion: The odontogenesis of one-day-old C57BL/6 mice in this study were observed in the early bell stage both in the left maxillary and left mandibular molars. Based on this study, one-day-old C57BL/6 mice can be used as an alternative object of research in analyzing the tooth structure that have been developed in the early bell stage, both normal and pathological conditions."

"ABSTRAK Latar Belakang: Berbagai material kaping pulpa berbahan dasar kalsium silikatterus dikembangkan, diantaranya semen berbasis kalsium silikat denganpenambahan steroid. Tujuan: Menganalisis efek penambahan steroid pada semenberbasis kalsium silikat terhadap viabilitas sel fibroblas. Metode: Sel fibroblasembrio ayam direndam dalam ekstrak larutan semen berbasis kalsium silikatdengan penambahan steroid dan MTA. Viabilitas sel dihitung dengan menggunakan uji MTT. Hasil: Terdapat perbedaan bermakna (p ≤ 0,05)viabilitas sel pada kelompok semen berbasis kalsium silikat dengan penambahansteroid dibandingkan kelompok kontrol dan MTA. Kesimpulan: Penambahansteroid menurunkan viabilitas sel. Terdapat peningkatan pada 72 jam, yang menandakan terjadinya proliferasi sel.ABSTRACT Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation.;Background: Calcium silicate based materials are being developed continuously,one of them is calcium silicate based cement containing steroid. Objective: Toanalyze the effect of steroid addition in calcium silicate based cement onfibroblast cells viability. Methods: Chicken embryonic fibroblast cells wereimmersed in extract solution of calcium silicate based cement containing steroidand MTA. Viability was analyzed by MTT Assay. Results: Significant difference (p ≤ 0,05) of viability on calcium silicate based cement containing steroid groupwas found, compared to control and MTA group. Conclusion: Steroid additiondecrease viability. There was an increase in 72 hours, marking cells proliferation."

"ABSTRAK Latar Belakang: MTA bersifat biokompatibel dan dapat digunakan untuk perawatan kaping pulpa. Saat ini dikembangkan semen berbasis kalsium silikat sama seperti MTA dengan penambahan steroid, yaitu Odontocem. Tujuan:Membandingkan efek toksisitas odontocem dan MTA-Angelus terhadap viabilitas sel fibroblas. Metode:Sel fibroblast embrio ayam direndam dalam larutan odontocemdan dan MTA-Angeluspada 24 dan 72 jam. Viabilitas sel dihitung menggunakanMTT Assay. Hasil:Pada kelompok odontcemdan MTA-Angelus, terdapat perbedaan bermakna (p≤0,05 ) dibandingkan dengan kontrol. Pada paparan 24 jam, tidak terdapat perbedaan bermakna antara odontocem dengan MTA-Angelus. Kesimpulan:Odontocem dan dan MTA-Angelus menurunkan viabilitas sel pada 24 jam dan meningkatkan pada 72 jam.ABSTRACT Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours."

"ABSTRAK Latar Belakang. P.gingivalis merupakan bakteri periodonsium yang dapatberpindah ke saluran akar pada lesi endoperio kombinasi murni. Jumlah danproporsinya berperan terhadap keparahan penyakit karena sifat virulensinya.Tujuan: Menganalisis jumlah dan proporsi P.gingivalis saluran akar dan poketlesi endoperio kombinasi murni, lesi endo primer, dan lesi perio primer. Metode:16 sampel diambil dari 3 kelompok, kuantifikasi dengan PCR real-time. Hasil:Jumlah P.gingivalis terbesar pada poket lesi perio primer, proporsi P.gingivalisterbesar pada saluran akar lesi endo primer. Jumlah dan proporsi P.gingivalistidak berbeda bermakna antara saluran akar dan poket lesi endoperio kombinasimurni. Kesimpulan. Jumlah dan proporsi P.gingivalis sebanding antara saluran akar dan poket lesi endoperio kombinasi murni.ABSTRACT Background : P.gingivalis is a periodontium pathogen that can be transferred tothe root canal of the true combined endoperio lesions. Quantity and proportiontakes part in the severity of the disease because of its virulence. Objective: toanalyze the quantity and proportion of P.gingivalis in the root canals and pocketof true combined endoperio lesions, primary endodontic lesions, and primaryperiodontal lesions. Methods 16 samples were taken from 3 groups,quantification with real-time PCR. Results : P.gingivalis largest quantity wasfound in pockets of primary periodontal lesions, P.gingivalis largest proportionwas found in root canals of primary endodontic lesions. There were no significantdifferences in quantity and proportion of P.gingivalis in root canals and pockets oftrue combined endoperio lesions. Conclusion. Quantity and proportion ofP.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.;Background : P.gingivalis is a periodontium pathogen that can be transferred tothe root canal of the true combined endoperio lesions. Quantity and proportiontakes part in the severity of the disease because of its virulence. Objective: toanalyze the quantity and proportion of P.gingivalis in the root canals and pocketof true combined endoperio lesions, primary endodontic lesions, and primaryperiodontal lesions. Methods 16 samples were taken from 3 groups,quantification with real-time PCR. Results : P.gingivalis largest quantity wasfound in pockets of primary periodontal lesions, P.gingivalis largest proportionwas found in root canals of primary endodontic lesions. There were no significantdifferences in quantity and proportion of P.gingivalis in root canals and pockets oftrue combined endoperio lesions. Conclusion. Quantity and proportion ofP.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.;Background : P.gingivalis is a periodontium pathogen that can be transferred tothe root canal of the true combined endoperio lesions. Quantity and proportiontakes part in the severity of the disease because of its virulence. Objective: toanalyze the quantity and proportion of P.gingivalis in the root canals and pocketof true combined endoperio lesions, primary endodontic lesions, and primaryperiodontal lesions. Methods 16 samples were taken from 3 groups,quantification with real-time PCR. Results : P.gingivalis largest quantity wasfound in pockets of primary periodontal lesions, P.gingivalis largest proportionwas found in root canals of primary endodontic lesions. There were no significantdifferences in quantity and proportion of P.gingivalis in root canals and pockets oftrue combined endoperio lesions. Conclusion. Quantity and proportion ofP.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.;Background : P.gingivalis is a periodontium pathogen that can be transferred tothe root canal of the true combined endoperio lesions. Quantity and proportiontakes part in the severity of the disease because of its virulence. Objective: toanalyze the quantity and proportion of P.gingivalis in the root canals and pocketof true combined endoperio lesions, primary endodontic lesions, and primaryperiodontal lesions. Methods 16 samples were taken from 3 groups,quantification with real-time PCR. Results : P.gingivalis largest quantity wasfound in pockets of primary periodontal lesions, P.gingivalis largest proportionwas found in root canals of primary endodontic lesions. There were no significantdifferences in quantity and proportion of P.gingivalis in root canals and pockets oftrue combined endoperio lesions. Conclusion. Quantity and proportion ofP.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions.;Background : P.gingivalis is a periodontium pathogen that can be transferred tothe root canal of the true combined endoperio lesions. Quantity and proportiontakes part in the severity of the disease because of its virulence. Objective: toanalyze the quantity and proportion of P.gingivalis in the root canals and pocketof true combined endoperio lesions, primary endodontic lesions, and primaryperiodontal lesions. Methods 16 samples were taken from 3 groups,quantification with real-time PCR. Results : P.gingivalis largest quantity wasfound in pockets of primary periodontal lesions, P.gingivalis largest proportionwas found in root canals of primary endodontic lesions. There were no significantdifferences in quantity and proportion of P.gingivalis in root canals and pockets oftrue combined endoperio lesions. Conclusion. Quantity and proportion ofP.gingivalis were comparable between the root canals and pockets of true combined endoperio lesions."

"ABSTRAK Latar Belakang: E.faecalis merupakan bakteri saluran akar yang dapat bermigrasi ke jaringan periodonsium pada lesi endo-perio kombinasi murni.Jumlahnya berperan terhadap keparahan penyakit karena sifat virulensinya. Tujuan: Menganalisis tipe strain dan jumlah E.faecalis di saluran akar dan poketlesi endo-perio kombinasi murni, lesi endo primer, dan lesi perio primer. Metode:16 sampel dari 3 kelompok, analisis strain dengan sequencing dan kuantifikasidengan Real Time PCR. Hasil: Terdapat persamaan strain E.faecali,s jumlahterbesar terdapat pada poket lesi endo-perio kombinasi murni. Tidak berbedabermakna antara saluran akar dan poket pada lesi endo-perio kombinasi murni.Kesimpulan; terdapat strain yang sama dan jumlah E.faecalis sebanding antarasaluran akar dan poket lesi endo-perio kombinasi murni.ABSTRACT Background: E.faecalis is a root canal pathogen that can migrate to periodontal tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part inthe severity of the disease because of its virulence. Objective: To analyze thestrain type and quantity of E.faecalis taken from root canal and pocket of truecombined endo-perio lesion, primary endodontic lesion and primary periodontallesion. Methods: 16 samples were taken from 3 groups, strain type was analyzedby sequencing and quantification by Real Time PCR. Results: There weresimilarities of E. faecalis strain. The largest number were found in the pockets oftrue combined endo-perio lesion. There were no significant differences betweenroot canal and pocket of true combined endo-perio lesions. Conclusion: Therewere similarities strain and quantity of E. faecalis were equal between root canaland pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part inthe severity of the disease because of its virulence. Objective: To analyze thestrain type and quantity of E.faecalis taken from root canal and pocket of truecombined endo-perio lesion, primary endodontic lesion and primary periodontallesion. Methods: 16 samples were taken from 3 groups, strain type was analyzedby sequencing and quantification by Real Time PCR. Results: There weresimilarities of E. faecalis strain. The largest number were found in the pockets oftrue combined endo-perio lesion. There were no significant differences betweenroot canal and pocket of true combined endo-perio lesions. Conclusion: Therewere similarities strain and quantity of E. faecalis were equal between root canaland pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part inthe severity of the disease because of its virulence. Objective: To analyze thestrain type and quantity of E.faecalis taken from root canal and pocket of truecombined endo-perio lesion, primary endodontic lesion and primary periodontallesion. Methods: 16 samples were taken from 3 groups, strain type was analyzedby sequencing and quantification by Real Time PCR. Results: There weresimilarities of E. faecalis strain. The largest number were found in the pockets oftrue combined endo-perio lesion. There were no significant differences betweenroot canal and pocket of true combined endo-perio lesions. Conclusion: Therewere similarities strain and quantity of E. faecalis were equal between root canaland pocket true combined endo-perio lesions. ;Background: E.faecalis is a root canal pathogen that can migrate to periodontal tissue in true combined endo-perio lesion. The quantity of E.faecalis takes part inthe severity of the disease because of its virulence. Objective: To analyze thestrain type and quantity of E.faecalis taken from root canal and pocket of truecombined endo-perio lesion, primary endodontic lesion and primary periodontallesion. Methods: 16 samples were taken from 3 groups, strain type was analyzedby sequencing and quantification by Real Time PCR. Results: There weresimilarities of E. faecalis strain. The largest number were found in the pockets oftrue combined endo-perio lesion. There were no significant differences betweenroot canal and pocket of true combined endo-perio lesions. Conclusion: Therewere similarities strain and quantity of E. faecalis were equal between root canaland pocket true combined endo-perio lesions. "

"ABSTRAK Latar Belakang : Enterococcus faecalis merupakan bakteri yang mampu membentuk biofilm dan banyak ditemukan pada kasus kelainan periapeks.Tujuan : Melihat daya antibakteri campuran triantibiotik dibandingkan denganklorheksidin 2% terhadap E. faecalis dalam biofilm. Metode : Menilai kekeruhanlarutan E. faecalis dalam biofilm pasca pemaparan bahan uji, dengan ELISAreader. Hasil : Terdapat daya antibakteri campuran triantibiotik terhadap biofilmbakteri E. faecalis tetapi tidak terdapat perbedaan bermakna dengan klorheksidin2% (p>0.05). Kesimpulan : Daya antibakteri campuran triantibiotik terhadapbiofilm E. faecalis sebanding dengan klorheksidin 2%. ABSTRACT Background : Enterococcus faecalis has the ability to form biofilm and is oftenfound in cases of periapical lesions. Aim: To analyze the effectivity of triantibioticmixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :Score the turbidity of E. faecalis in biofilm after immersion in antibacterialagent, with ELISA reader. Result : Triantibiotic mixture has antibacterialeffectivity against E. faecalis biofilm but has no significant difference compared to2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibioticmixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is oftenfound in cases of periapical lesions. Aim: To analyze the effectivity of triantibioticmixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :Score the turbidity of E. faecalis in biofilm after immersion in antibacterialagent, with ELISA reader. Result : Triantibiotic mixture has antibacterialeffectivity against E. faecalis biofilm but has no significant difference compared to2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibioticmixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is oftenfound in cases of periapical lesions. Aim: To analyze the effectivity of triantibioticmixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :Score the turbidity of E. faecalis in biofilm after immersion in antibacterialagent, with ELISA reader. Result : Triantibiotic mixture has antibacterialeffectivity against E. faecalis biofilm but has no significant difference compared to2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibioticmixture against E. faecalis biofilm is equal to2% chlorhexidine. ;Background : Enterococcus faecalis has the ability to form biofilm and is oftenfound in cases of periapical lesions. Aim: To analyze the effectivity of triantibioticmixture compared to 2% chlorhexidine against biofilm of E. faecalis. Method :Score the turbidity of E. faecalis in biofilm after immersion in antibacterialagent, with ELISA reader. Result : Triantibiotic mixture has antibacterialeffectivity against E. faecalis biofilm but has no significant difference compared to2% chlorhexidine (p>0.05). Conclusion : Antibacterial effectivity of triantibioticmixture against E. faecalis biofilm is equal to2% chlorhexidine. "