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"Growth differentiation factor 9 (GDF9) dan bone morphogenetic protein 15 (BMP15) merupakan growth factor yang disekresikan oosit yang berperan penting dalam meningkatkan pertumbuhan awal folikel serta pematangan oosit. Keberadaan protein GDF9 dan BMP15 tidak hanya terdapat pada oosit, tetapi juga pada sel granulosa. Penelitian bertujuan untuk mengetahui ekspresi gen GDF9 dan BMP15 pada sel granulosa manusia. Sel granulosa yang diteliti berasal dari 30 pasien yang menjalani fertilisasi in vitro (FIV). Hasil isolasi RNA dari sel granulosa diubah menjadi cDNA melalui reaksi reverse transcription, lalu ekspresi gen GDF9 dan BMP15 diukur dengan QRT-PCR. Hasil penelitian menunjukkan bahwa sebagian besar sampel tidak mengekspresikan gen GDF9 dan BMP15. Beberapa sampel yang menunjukkan ekspresi gen GDF9 dan BMP15 pada sel granulosa memiliki tingkat ekspresi yang bervariasi pada tiap sampel. Hasil penelitian menunjukkan bahwa gen GDF9 lebih banyak diekspresikan pada sel granulosa manusia dibandingkan gen BMP15.

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Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are oocyte-derived growth factors that have important role to improve early follicle growth and oocyte maturity. Localization of GDF9 and BMP15 protein are not only in oocyte, but also in granulosa cells. Purpose of the research is to find out expression level of GDF9 and BMP15 genes in human granulosa cells. Granulosa cells were collected from 30 patients undergoing in vitro fertilization (IVF) treatment. Total RNA was isolated from the cells and reverse transcribed to cDNA via reverse transcription reaction, and then GDF9 and BMP15 genes expression were quantified using QRT-PCR. Result of this research is granulosa samples majority did not expressed GDF9 and BMP15 genes. Only several samples shown GDF9 and BMP15 genes expression which have vary expression level in each sample. Based on the result, GDF9 gene more expressed in human granulosa cells than BMP15 gene."

"Demam Dengue (DD) dan Demam Berdarah Dengue (DBD) adalah penyakit yang tersebar luas. Penelitian ini dilakukan untuk mengembangkan kandidat vaksin dengue nasional berbasis protein sub unit prM/E DEN-4. Protein prM/E adalah kompleks unik yang berperan penting dalam perakitan virus dan modulasi fusi. Penelitian telah dilakukan dengan metode Gateway cloning system untuk menklon gen prM/E dalam plasmid cloning pDONR221 kemudian dilakukan subkloning dan dipindahkan gen prM/E ke dalam plasmid eksperesi pET-55-DEST. Ekspresi protein prM/E dilakukan di dalam E.coli BL21 (DE3) dengan induksi Isoprophyl-β-D-thiogalactopyranoside (IPTG). Pendeteksian poliprotein Gag hasil ekspresi dilakukan dengan metode Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Setelah protein prM/E berhasil dideteksi kemudian protein prM/E dipurifikasi dengan menggunakan metode immobilized metal affinity chromatography (IMAC) di bawah kondisi denaturasi. Hasil penelitian yaitu protein prM/E dapat diekspresikan dalam E.coli BL21 (DE3) dengan berat molekul ~75 kDa.

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Dengue Fever is an infectious disease caused by one of the four serotypes of dengue virus (DENV). Until now, no licensed vaccines or antivirus is available commercially. Because of that, this research was aimed to develop candidate vaccine dengue based on protein subunit pre-membrane and Envelope (prM/E). Protein prM/E is a unique complex which has important role in virus assemblyand host cell entry. The recombinant protein development was done using Gateway cloning system. This was used to clone the prM/E gene into pDONR221 plasmid. The cloned gene was then transferred into pET-55-DEST expression plasmid. Expression of protein prM/E was perfomed in E. coli BL21 (DE3) with inducer Isoprophyl-β-D-thiogalactopyranoside (IPTG). Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE) method was used to detect the expressed prM/E protein. Upon detection of prM/E protein with SDS-PAGE, the recombinant protein was purified by using immobilized metal affinity chromatography (IMAC) method under denature condition. Using these methods, the prM/E protein was successfully expressed in E.coli BL21 (DE3) with a molecular weight ~75 kDa."

"Pemilihan oosit merupakan salah satu tahap penting dalam fertilisasi in vitro (FIV) sebab semakin banyak jumlah oosit yang masuk kualifikasi, maka semakin besar kesempatan oosit tersebut untuk difertilisasi. Growth Differentiation Factor 9 (GDF9) dan Bone Morphogenetic 15 (BMP15) yang merupakan anggota dari superfamili protein Transforming Growth Factor β (TGF-β), memiliki peranan penting terhadap folikulogenesis. Gen gdf9 dan bmp15 diketahui tidak hanya terekspresikan pada oosit, namun juga pada sel granulosa dan cairan folikel. Oleh karena itu, dilakukan penelitian mengenai ekspresi gen gdf9 dan bmp15 pada sel granulosa dan cairan folikel untuk mengetahui pengaruhnya terhadap kualitas oosit. Lima belas sampel sel granulosa dan cairan folikel dikumpulkan untuk mengukur ekspresi gen gdf9 dan bmp15 secara kuantitatif. Metode kuantifikasi absolut digunakan untuk mengukur tingkat ekspresi gen. Uji korelasi Pearson digunakan untuk menganalisis korelasi antara ekspresi gen gdf9 dan bmp15 terhadap parameter umum fisiologis. Ekspresi gen gdf9 pada sel granulosa memiliki korelasi lemah searah tak signifikan (P>0,05) terhadap umur, tingkat kematangan oosit, tingkat fertilisasi normal, dan tingkat pembelahan. Ekspresi gen bmp15 memiliki korelasi lemah searah tak signifikan (P>0,05) terhadap umur serta memiliki korelasi lemah tak searah tak signifikan (P>0,05) terhadap tingkat kematangan oosit, tingkat fertilisasi normal, dan tingkat pembelahan. Ekspresi gen gdf9 dan bmp15 pada cairan folikel tidak dapat dianalisis sebab kurva standar tidak terkonstruksi.

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Oocyte selection is one of important steps in in vitro fertilization (IVF) procedures, because more number of qualified oocyte will bring its chance to be fertilized bigger. Growth Differentiation Factor 9 (GDF9) dan Bone Morphogenetic 15 (BMP15) are members of protein superfamily Transforming Growth Factor β (TGF-β) which have important role in folliculogenesis. Gdf9 dan bmp15 genes are not only expressed in oocyte, but in granulosa cell and follicular fluid too. Research has been conducted about gdf9 and bmp15 gene expression in granulosa cell and follicular fluid to know their association with oocyte quality. Fifteen samples of granulosa cell and follicular fluid were collected to measure gdf9 and bmp15 gene expression quantitatively. Absolute quantification method were used to measure gene expression levels. Pearson correlation was used to analize correlation between both gene expression levels and general physiological parameters. The expression levels of gdf9 in granulosa cell had weak positive unsignificant (P>0,005) correlation with age, oocyte maturity rate, normal fertilization rate, and cleavage rate. The expression levels of bmp15 in granulosa cell had weak positive unsignificant (P>0,005) correlation with age, but had weak negative unsignificant (P>0,005) correlation with oocyte maturity rate, normal fertilization rate, and cleavage rate. Gdf9 and bmp15 gene expression in follicular fluid cannot be analized because the standard curve could not be constructed."

"Dimetil ftalat (DMP), salah satu jenis bahan aditif, umum ditambahkan untuk meningkatkan fleksibilitas, kekuatan, dan kegunaan bahan polimer. BHA merupakan senyawa sintesis yang umum ditambahkan ke dalam bahan pangan dan produk lain yang mengandung minyak atau lemak. DMP dan BHA mampu menginduksi stres oksidatif dan meningkatkan risiko munculnya berbagai penyakit. Penelitian ini bertujuan untuk menganalisis DNA adduct 8-OHdG (suatu biomarker kerusakan DNA) secara in vitro dan in vivo pada tikus. Studi in vitro dilaksanakan dengan melakukan inkubasi terhadap 2-deoksiguanosin (2-dG) dengan multikomponen DMP, BHA, dan Ni(II) dengan variasi pH (7,4 dan 8,4) menggunakan suhu 37ºC. HPLC digunakan untuk menganalisis hasil 8-OHdG yang terbentuk. Studi in vivo dilaksanakan dengan menggunakan tikus yang diberikan paparan multikomponen DMP, BHA, dan Ni(II) dengan lama periode 28 hari melalui jalur oral (ingesti). Sampel darah dikumpulkan sebanyak dua kali per satu minggu kemudian dianalisis dengan ELISA Kit untuk menguji tingkat 8-OHdG yang terbentuk. Hasil dari penelitian ini diketahui bahwa paparan multikomponen DMP, BHA, dan Ni(II) menghasilkan pembentukan 8-OHdG yang lebih tinggi dibandingkan tanpa paparan. Pada kondisi pH 7,4 dalam studi in vitro, terjadi peningkatan kadar pembentukan 8-OHdG dibandingkan pH 8,4.

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Dimethyl phthalate (DMP), a type of additive, is commonly added to enhance the flexibility, strength, and utility of polymer materials. BHA is a synthetic compound commonly added to food products and other items containing oil or fat. DMP and BHA are capable of inducing oxidative stress and increasing the risk of various diseases. This study aims to analyze the DNA adduct 8-OHdG (a biomarker of DNA damage) both in vitro and in vivo in rats. The in vitro study was conducted by incubating 2-deoxyguanosine (2-dG) with multicomponent DMP, BHA, and Ni(II) with variations in pH (7.4 and 8.4) at 37ºC. HPLC was used to analyze the resulting 8-OHdG formation. The in vivo study was conducted using rats exposed to multicomponent DMP, BHA, and Ni(II) for a period of 28 days via oral ingestion. Blood samples were collected twice per week and then analyzed using an ELISA Kit to test the levels of 8-OHdG formed. The results of this study indicated that exposure to multicomponent DMP, BHA, and Ni(II) resulted in higher 8-OHdG formation compared to no exposure. Under pH 7.4 conditions in the in vitro study, there was an increase in 8-OHdG formation compared to pH 8.4."

"Infertilitas merupakan ketidakmampuan pasangan suami istri dalam memeroleh keturunan selama rentang waktu satu tahun tanpa adanya hal yang menghalangi fertilisasi. Infertilitas dapat diatasi dengan ART, yang salah satunya ialah prosedur FIV. Dalam penelitian ini, ingin diketahui hubungan antara ekspresi gen LHR di sel granulosa dengan rasio keberhasilan fertilisasi. Rasio ekspresi gen LHR diestimasi dengan metode qRT-PCR. Hasil analisis pada 30 sampel, hanya 20 sampel yang berhasil di ketahui rasio ekspresi LHRnya. Ditemukan korelasi negatif tak bermakna (r=-0,174, p=0,463) antara gen LHR dengan rasio keberhasilan fertilisasi. Dari analisis statistik deskriptif, didapatkan rerata kelompok rasio fertilisasi rendah 2,01±1,51(arbitary unit), kelompok rasio fertilisasi sedang 5,69±7,02 (arbitary unit), kelompok rasio fertilisasi tinggi 3,93±4,90 (arbitary unit). Perlu dilakukan analisis terhadap ekspresi reseptor lain yang berkaitan dengan perkembangan dan pematangan oosit untuk mendapatkan pengetahuan yang lebih komprehensif.

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Infertility can be defined as the inability of the couple to achive a pregnancy over within one year of regular unprotected intercourse. Infetility can be overcome by ART, which one of them is IVF procedures. In this study, we want to know the relationship between LHR gene expression in granulosa cells with fertilization rate. LHR gene expression ratios was estimated by qRT - PCR. There are only 20 of 30 samples were successful to express LHR gene. Statistical analysis shown a very weak negative correlation between LHR genes expression and fertilization rate (r = -0.174, p=0.463). From the descriptive statistical analysis, the group which obtained a lowest mean ratio is low fertilization rate group (2.01±1.51 arbitrary units), the highest expression of LHR is medium fertilization rate group (5.69±7.02 arbitary units), and the high fertilization rate group express LHR gene 3.93 ± 4.90 arbitrary units. Futher analysis on another gene which contributes in follicular development is needed to get comprehensive knowledge about oocyte maturation."

"Acquired Immunodeficiency Syndrome (AIDS) merupakan penyakit akibat infeksi Human Immunodeficiency Virus (HIV) yang memiliki jumlah penderita tinggi di Indonesia. Salah satu upaya untuk mencegah bertambahnya jumlah penderita AIDS tersebut ialah dengan penggunaan vaksin. Poliprotein Gag merupakan protein penyusun struktur internal HIV yang dapat digunakan sebagai vaksin karena dapat menginduksi respon imun tubuh. Penelitian telah dilakukan untuk mengekspresikan poliprotein Gag HIV-1 subtipe CRF01_AE yang telah diinsersi ke dalam vektor ekspresi pQE-80L. Ekspresi poliprotein tersebut dilakukan di dalam bakteri Escherichia coli BL21 dan E. coli BL21-CodonPlus (CP) dengan induksi Isoprophyl-β-D-thiogalactopyranoside (IPTG). Pendeteksian poliprotein Gag hasil ekspresi dilakukan dengan metode Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Setelah poliprotein berhasil dideteksi, poliprotein Gag kemudian dipurifikasi dengan menggunakan metode kromatografi afinitas Ni2+-NTA di bawah kondisi native. Poliprotein Gag HIV-1 subtipe CRF01_AE dapat diekspresikan dalam E. coli BL21 dan E. coli BL21-CP dengan berat molekul sebesar 55,3 kDa.

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Acquired Immunodeficiency Syndrome (AIDS) is a disease caused by infection of Human Immunodeficiency Virus (HIV) which has a high number of people in Indonesia. One of the efforts to prevent the increasing number of AIDS patients is the use of vaccine. Gag polyprotein is a constituent protein of HIV internal structure that can be used as a vaccine because it can induce immune response of the body. Research has been conducted to express the Gag polyprotein HIV-1 subtype CRF01_AE that have been cloned into the pQE-80L expression vector. Polyprotein expression was carried out in Escherichia coli BL21 and E. coli BL21-CodonPlus (CP) with Isoprophyl-β-Dthiogalactopyranoside (IPTG) induction. Detection of Gag polyprotein was performed by Polyacrilamide Sodium Dodecyl Sulphate Gel Electrophoresis (SDS-PAGE) method. After successfully detected, Gag polyprotein then purified using Ni2+-NTA affinity chromatography under native condition. Gag polyprotein HIV-1 subtype CRF01_AE can be expressed in E. coli BL21 and E. coli BL21-CP with molecular weight is 55,3 kDa."

"Kanker kolorektal adalah penyakit neoplasma ganas yang tumbuh dan berkembang pada saluran usus besar dan atau rektum. Terapi anti EGFR menggunakan agen biologis antibodi monoklonal cetuximab dan panitumumab diketahui memberikan tingkat penyembuhan yang baik pada pasien kanker kolorektal. Pasien dengan mutasi pada gen NRAS dan KRAS cenderung resisten terhadap terapi anti EGFR, sehingga penting dilakukan pemeriksaan kedua gen tersebut sebelum pemberian terapi. Pemeriksaan gen NRAS belum tersedia di Indonesia karena minimnya data mengenai mutasi gen tersebut pada populasi Indonesia. Penelitian dilakukan untuk mengetahui profil mutasi pasien gen NRAS pada 58 sampel pasien kanker kolorektal di Jakarta. Pemeriksaan mutasi dilakukan pada exon 2(codon 12 & 13) dan exon 3 (codon 61) gen NRAS menggunakan metode sekuensing. Analisis elektroferogram sekuensing menunjukan mutasi gen NRAS ditemukan pada 6,9% (n=58) sampel uji. Hasil uji statistik fischer exact test dua arah menunjukan tidak adanya asosiasi mutasi gen dengan kelompok usia pasien dan jenis kelamin. Gen NRAS ditemukan termutasi pada codon 12 (1,7 %) dan codon 61 (5.2%). Tidak ditemukan adanya mutasi pada codon 13 gen NRAS.

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Colorectal cancer is a neoplasm disease that arise in inner lining of colon or rectum. Anti EGFR therapy such as cetuximab and panitumumab were widely used to suppress metastases colorectal cancer in patient and decided as gold standard therapy. Mutation either KRAS or NRAS gene will reduced effectifity of anti EGFR therapy, hence genotyping of KRAS and NRAS gene must be executed before. NRAS genotyping test not yet available in Indonesia due to lack of information about this gene. This study was subjected to understanding profile of NRAS gene mutation in Jakartans colorectal cancer patient. Mutation screening was perform by sequencing method, notably exon 2 (codon 12&13) and exon 3 (codon 61). Electropherograms analysis shows that NRAS mutation found in 6,9% samples (n=58). NRAS mutation found in codon 12 (1,7%), codon 61 (5,2%), and there was no mutation found in codon 13. Fischer exact test statistical analysis summarized that there was no significant association of NRAS mutation with both sex and age."

"Seng (Zn) diketahui dapat menginduksi apoptosis terhadap galur sel kanker prostat (PC-3). Akumulasi selular Seng (Zn) memiliki efek langsung pada mitokondria yang menghasilkan pelepasan sitokrom c yang memicu apoptosis. Tujuan dari penelitian ialah mengevaluasi pengaruh Seng (Zn) terhadap viabilitas PC-3 dengan konsentrasi 10 µM, 15 µM, 20 µM, dan staurosporin sebagai kontrol pada waktu pemajanan 0, 6, dan 24 jam. Viabilitas sel-sel tersebut diukur dengan uji MTS. Hasil uji Kruskal Wallis menunjukkan bahwa tidak terdapat perbedaan bermakna antara kelompok kontrol dan ketiga kelompok perlakuan. Namun, terdapat perbedaan bermakna antara perlakuan stauropsorin dibandingkan kontrol tanpa perlakuan. Dengan demikian, konsentrasi Zn 10 µM, 15 µM, dan 20 µM pada pemaparan 0 jam, 6 jam, dan 24 jam belum dapat menurunkan viabilitas sel kanker prostat PC-3 secara signifikan.

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Zinc (Zn), known to trigger apoptosis in prostate cancer line (PC-3), has direct effect on mitochondria and the release of cytochrome c leading to apoptosis. The aim of this study was to evaluate the role of Zinc in Prostate cancer cell line (PC-3) viability with concentrations of 10, 15, 20 µM, and staurosporine as a control, and exposure time of 0, 6, and 24 hours. The cell viability was assessed with MTS assay. There were no significant differences between control and treatment groups based on Kruskal Wallis test. However, there was significant difference between stauroporine treatment and negative control. In conclusion, Zinc (Zn) concentration of 10, 15, 20 µM and exposure time of 0, 6, and 24 hours were not sufficient to decrease significantly the viability of prostate cancer lines (PC-3)."

"[Penicillium marneffei merupakan fungi patogen yang ditemukan di AsiaTenggara, khususnya Thailand. Penisiliosis dapat menyebabkan mikosis sistemiksehingga membahayakan nyawa penderita immunocompromised, khususnyapenderita HIV/AIDS. Antifungi seperti Fluconazole dan Ketoconazole, digunakanuntuk mengatasi infeksi P. marneffei. Akan tetapi, penggunaan antifungi secarajangka panjang dapat memicu kemungkinan munculnya mutan resisten P.marneffei. Resistensi pada fungi dapat dipengaruhi beberapa faktor, salah satunya,overekspresi transporter pengeluaran obat (drug efflux transporter). Mekanismepompa pengeluaran obat diatur oleh berbagai transporter. Transporter yang palingumum diketahui ialah transporter ABC (ATP-binding-cassette) dan MFS (MajorFacilitator Superfamily). Transporter ABC multidrug (MDR) pada P. marneffeitelah dipelajari dengan baik, sedangkan transporter MFS MDR pada fungitersebut, belum mendapatkan perhatian yang sama. Penelitian ini fokus pada satutransporter MFS MDR P. marneffei, yakni PMAA 067100, yang diekspresikanpada Saccharomyces cerevisiae ADΔ; sistem ekspresi yang sangat rentan terhadapberbagai macam antifungi. Pengamatan melalui mikroskop konfokal dan uji DiskDiffusion menunjukkan bahwa transporter PMAA 067100 terlokalisasi padamembran sel S. cerevisiae ADΔ dan resisten terhadap Fluconazole danTerbinafine.;Penicillium marneffei has been known as a pathogenic fungi which isfound in Southeast Asia, especially Thailand. The infection by this fungirecognized as Penicilliosis, that caused systemic mycosis, might be lethal inimmunocompromised patient, specifically HIV/AIDS patient. Antifungal such asFluconazole and Ketoconazole, had been used against P. marneffei infection.However, the long-term-use of antifungal might cause an emerging resistant strainof P. marneffei. The resistance phenomenon in fungi is caused by several factors,one of it is the overexpression of drug efflux transporter. Mechanism of this effluxpump is regulated by some of transporters such as ABC (ATP-binding-cassette)and MFS (Major Facilitator Superfamily) transporter. The ABC multidrug (MDR)transporter of P. marneffei has been studied well, yet the underrated MFS MDRtransporter of the same fungi has not received the same attention. This study focuson one of P. marneffei MFS MDR transporter, known as PMAA 067100, whichwas expressed in Saccharomyces cerevisiae ADΔ; an expression system which isvery susceptible to many kind of antifungal. Observation through confocalmicroscope and Disk Diffusion test showed that PMAA 067100 transporter waslocalized in S. cerevisiae ADΔ cell membrane and resistant against Fluconazoleand Terbinafine., Penicillium marneffei has been known as a pathogenic fungi which isfound in Southeast Asia, especially Thailand. The infection by this fungirecognized as Penicilliosis, that caused systemic mycosis, might be lethal inimmunocompromised patient, specifically HIV/AIDS patient. Antifungal such asFluconazole and Ketoconazole, had been used against P. marneffei infection.However, the long-term-use of antifungal might cause an emerging resistant strainof P. marneffei. The resistance phenomenon in fungi is caused by several factors,one of it is the overexpression of drug efflux transporter. Mechanism of this effluxpump is regulated by some of transporters such as ABC (ATP-binding-cassette)and MFS (Major Facilitator Superfamily) transporter. The ABC multidrug (MDR)transporter of P. marneffei has been studied well, yet the underrated MFS MDRtransporter of the same fungi has not received the same attention. This study focuson one of P. marneffei MFS MDR transporter, known as PMAA 067100, whichwas expressed in Saccharomyces cerevisiae ADΔ; an expression system which isvery susceptible to many kind of antifungal. Observation through confocalmicroscope and Disk Diffusion test showed that PMAA 067100 transporter waslocalized in S. cerevisiae ADΔ cell membrane and resistant against Fluconazoleand Terbinafine.]"

"Protein APOBEC3G merupakan salah satu protein intrinsik sel imun manusia yang memiliki kemampuan antiretroviral terhadap infeksi HIV-1. Protein Vif HIV-1 dapat merangsang degradasi APOBEC3G sehingga penghambatan interaksi antara kedua protein tersebut melalui inhibitor berpotensi digunakan dalam pengembangan antiretroviral baru. Pengembangan antiretroviral baru melalui interaksi antara protein APOBEC3G dengan protein Vif HIV-1 memerlukan protein rekombinan APOBEC3G. Protein APOBEC3G diperoleh dengan cara melakukan ekspresi gen APOBEC3G yang telah dikloning ke dalam vektor ekspresi pQE-80L. Ekspresi protein APOBEC3G dilakukan dalam sistem ekspresi prokariota yaitu bakteri Escherichia coli BL21-CodonPlus (DE3). Ekspresi protein dilakukan pada tiga kondisi optimasi yaitu suhu, konsentrasi IPTG dan waktu inkubasi setelah induksi. Berdasarkan penelitian, APOBEC3G berukuran 43,08 kDa dapat diekspresikan paling optimal pada induksi IPTG 0,5 mM pada suhu 37oC waktu inkubasi 4 jam setelah induksi.

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APOBEC3G is one of intrinsic protein in human immune system. It has an antiretroviral ability against HIV-1 infection. However, HIV-1 has Vif protein which stimulate degradation of APOBEC3G. Therefore, inhibition of interaction between both proteins by an inhibitor is potentially used in development of new antiretroviral agents. The development of new antiretroviral using interaction of APOBEC3G and Vif HIV-1 needs recombinant protein of APOBEC3G. This APOBEC3G recombinant protein can be produced by expression of APOBEC3G gene cloned into pQE-80L expression vector in prokaryotic system of Escherichia coli BL21-CodonPlus (DE3). Protein expression conducted in three optimization condition: themperature, IPTG concentration, and incubation time after induction. Based on this research, APOBEC3G which has 43,08 kDa molecular weight is expressed optimally in 0,5 mM IPTG induction at 37oC and incubation time 4 hours after induction."