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"Latar Belakang: Salah satu kunci keberhasilan perawatan regenerasi endodontik adalah disinfeksi dari sistem saluran akar. Bahan irigasi bersifat bakterisid dan mampu mempertahankan kelangsungan hidup sel punca.Tujuan: Membandingkan efek toksik larutan NaOCl 2.5%, EDTA 17%, dan CHX 2% terhadap viabilitas sel punca mesenkim pulpa.Metode: Kultur sel primer dari gigi molar ketiga imatur. Sel punca mesenkim pulpa dideteksi dengan marker STRO-1 menggunakan uji immunofluorescence. Sel dipaparkan dengan bahan uji dan viabilitas sel dihitung dengan uji MTT.Hasil: Terdapat perbedaan bermakna viabilitas sel punca mesenkim pulpa ketiga larutan dibandingkan kontrol (p ≤ 0.05). Tidak terdapat perbedaan bermakna viabilitas sel antar larutan (p ≥ 0.05).Kesimpulan: Ketiga larutan memiliki efek toksik terhadap viabilitas sel punca mesenkim pulpa.

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Background: One of the key to the success of regeneration endodontic treatment is the disinfection of the root canal system. Irrigation materials not only have bactericidal properties but also able to maintain the viability of stem cells.

Objective: To compare the toxic effects of NaOCl 2.5%, EDTA 17%, and CHX 2% solutions on the viability of dental pulp mesenchymal stem cells.

Methods: Primary cultures cells taken from immature third molars. Dental pulp mesenchymal stem cells was detected by STRO-1 marker using immunofluorescence assay. Cells were exposed to three solutions and cell viability was analyzed using the MTT assay.

Results: There were significant differences from the viability of dental pulp mesenchymal stem cells of three solutions when compared with controls (p ≤ 0.05). There were no significant differences from cell viability when compared between solutions (p ≥ 0.05).

Conclusion: All solutions have toxic effects on the viability of dental pulp mesenchymal stem cells."

"ABSTRAK Latar Belakang: siler polidimetilsiloksan merupakan siler baru yang berpotensi menutup saluran akar lebih baik. Tujuan: membandingkan tingkat kerapatanpengisian di sepertiga apeks antara teknik kon tunggal (KT) dan downpackbackfill(DB) menggunakan siler polidimetilsiloksan. Metode: empat puluh gigipremolar bawah dibagi menjadi dua kelompok KT dan DB. Setelah pengisiansaluran akar, sampel diinkubasi, dilapisi cat kuku, direndam dalam tinta India, laludibuat transparan dengan metode Robertson. Kebocoran di sepertiga apeksdievaluasi dengan mikroskop stereo. Hasil: skor kebocoran terendah (0-0,5 mm)dimiliki oleh kelompok KT dan skor kebocoran tertinggi (>1 mm) dimiliki olehkelompok DB. Kesimpulan: teknik kon tunggal memberikan tingkat kerapatanyang lebih baik dibandingkan downpack-backfill, walaupun secara statistik perbedaan antar keduanya tidak signifikan. ABSTRACT Background: polydimethylsiloxane sealer is a brand new sealer that potentiallyhas a better sealing ability to the root canal. Aim: to compare the apical sealingability in one third apex between single cone (SC) and downpack-backfill (DB)technique using polydimethylsiloxane sealer. Methods: fourty extracted humanmanibular premolars devided into two group, SC and DB. After obturated withpolydimethylsiloxane sealer, samples were stored in incubator, coated with nailvarnish, immersed in India ink, and then specimens were cleared usingRobertson?s technique. The apical dye penetration was evaluated using astereomicroscope. Result: the lowest leakage score (0-0,5 mm) was found in SCgroup and the highest score(>1 mm) in DB group. Summary: single conetechnique exibited better sealing ability rather than downpack-backfill technique, although no statistical significant difference between this two techniques. ;Background: polydimethylsiloxane sealer is a brand new sealer that potentiallyhas a better sealing ability to the root canal. Aim: to compare the apical sealingability in one third apex between single cone (SC) and downpack-backfill (DB)technique using polydimethylsiloxane sealer. Methods: fourty extracted humanmanibular premolars devided into two group, SC and DB. After obturated withpolydimethylsiloxane sealer, samples were stored in incubator, coated with nailvarnish, immersed in India ink, and then specimens were cleared usingRobertson?s technique. The apical dye penetration was evaluated using astereomicroscope. Result: the lowest leakage score (0-0,5 mm) was found in SCgroup and the highest score(>1 mm) in DB group. Summary: single conetechnique exibited better sealing ability rather than downpack-backfill technique, although no statistical significant difference between this two techniques. ;Background: polydimethylsiloxane sealer is a brand new sealer that potentiallyhas a better sealing ability to the root canal. Aim: to compare the apical sealingability in one third apex between single cone (SC) and downpack-backfill (DB)technique using polydimethylsiloxane sealer. Methods: fourty extracted humanmanibular premolars devided into two group, SC and DB. After obturated withpolydimethylsiloxane sealer, samples were stored in incubator, coated with nailvarnish, immersed in India ink, and then specimens were cleared usingRobertson?s technique. The apical dye penetration was evaluated using astereomicroscope. Result: the lowest leakage score (0-0,5 mm) was found in SCgroup and the highest score(>1 mm) in DB group. Summary: single conetechnique exibited better sealing ability rather than downpack-backfill technique, although no statistical significant difference between this two techniques. "

"ABSTRAK Latar Belakang: MTA bersifat biokompatibel dan dapat digunakan untuk perawatan kaping pulpa. Saat ini dikembangkan semen berbasis kalsium silikat sama seperti MTA dengan penambahan steroid, yaitu Odontocem. Tujuan:Membandingkan efek toksisitas odontocem dan MTA-Angelus terhadap viabilitas sel fibroblas. Metode:Sel fibroblast embrio ayam direndam dalam larutan odontocemdan dan MTA-Angeluspada 24 dan 72 jam. Viabilitas sel dihitung menggunakanMTT Assay. Hasil:Pada kelompok odontcemdan MTA-Angelus, terdapat perbedaan bermakna (p≤0,05 ) dibandingkan dengan kontrol. Pada paparan 24 jam, tidak terdapat perbedaan bermakna antara odontocem dengan MTA-Angelus. Kesimpulan:Odontocem dan dan MTA-Angelus menurunkan viabilitas sel pada 24 jam dan meningkatkan pada 72 jam.ABSTRACT Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours.;Background:MTA is proved tobe biocompatible and can be used for pulp capping treatment.Currently, calcium silicate based cement similar to MTA with steroid,called Odontocem has been developed.Objective:To compare effects of odontocem and MTA-Angelus toxicity towards fibroblast cells viability.Method:Fibroblast cells of chicken embryonic were immersed separately in odontocem and MTA-Angelus solution for 24 and 72 hours.Cells viability was analyzed with MTT Assay.Result:There was a significant difference (p>0.05) in Odontocem and MTA-Angelus group compared to control.At the24-hour immersion, there was nosignificant difference between odontocem and MTA-Angelus.Conclusion:Odontocem and MTA-Angelus decreased the viability of fibroblast at 24 hours and increased them at 72 hours."

"Latar Belakang: Penggunaan adhesif universal sebagai sementasi pasak fiber dalam restorasi pasca endodontik populer digunakan karena sifatnya yang serbaguna sehingga dapat diaplikasikan dengan metode total-etch maupun self-etch. Namun, penelitian mengenai perbedaan push-out bond strength (PBS) antara kedua metode tersebut masih terbatas. Tujuan: Mengetahui perbedaan PBS dan failure mode antara metode total-etch dan self-etch sistem adhesif universal pada sementasi pasak fiber. Metode: Tiga puluh gigi premolar rahang bawah yang baru diekstraksi disimpan di dalam air terdeionisasi yang kemudian dilakukan pemotongan mahkota sebelum dilakukan perawatan saluran akar dan preparasi ruang pasak. Pasak kemudian disementasi dengan semen resin dualcure self-adhesive dan bahan adhesif Prime&Bond Universal (PBU). Uji PBS dilakukan untuk mengukur kekuatan ikat geser masing-masing kelompok uji. Kegagalan ikatan kemudian diobservasi menggunakan Scanning Electron Microscopy (SEM). Hasil: Kelompok self-etch memiliki kekuatan ikat yang lebih tinggi dibandingkan dengan kelompok lainnya dengan perbedaan yang bermakna (p<0.05). Tidak terdapat perbedaan kegagalan adhesif yang signifikan di antara kelompok. Kesimpulan: Metode self-etch memiliki kekuatan ikat paling baik dibandingkan dengan kelompok uji lainnya, namun tidak terdapat perbedaan failure mode pada setiap kelompok.

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Background: Universal adhesives for fiber post cementation in endodontically treated teeth have become popular in clinical dentistry as they can be applied in either total-etch or self-etch mode, due to their reduced number of application steps and versatility. However, studies regarding comparison between the two modes are limited and insufficient. Aim/Objective: The aim of the study is to analyze and compare the pushout bond strength and failure mode of the multimode universal adhesive system. Materials and Methods: Thirty freshly extracted mandibular premolars were decoronated prior to a root canal treatment (RCT) and prepared for post placement and bonded using Prime&Bond Universal (PBU). Post was then cemented using dual-cure self-adhesive resin cement. The push-out bond strength (PBS) test was performed to measure the shear bond strength using a universal testing machine (UTM). Failure mode was then observed using scanning electron microscope (SEM). Results: One-way ANOVA followed by a post-hoc Bonferroni and Independent T-Test indicated that there is significant difference (p<0.05) between the bond strength of the total- and self-etch mode of the universal adhesive system. However, no differences in failure mode are observed between groups. Conclusion: Self-etch mode is more preferable due to its higher bond strength dominated with cohesive failure indicating its success in bonding with the root canal

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"ABSTRAKLatar Belakang Instrumen rotasi kontinyumemiliki banyak kekurangansehingga dikembangkan instrumen resiprokal Tujuan penelitian iniadalahmembandingkan kehalusan dinding sepertiga apikal saluran akarmenggunakan instrumen rotasi kontinyu dan resiprokal Metode Tiga puluh duapremolar pertama mandibula dibagi menjadi Kelompok 1 preparasi saluran akarmenggunakan instrumen rotasi kontinyu dan Kelompok 2 preparasi saluran akarmenggunakan instrumen resiprokal Kemudian kehalusan diukur dengan surfaceroughness tester Analisis data menggunakan uji T tidak berpasangan Hasil Tidak terdapat perbedaaan bermakna antara kedua kelompok p 0 739 Kesimpulan Dinding sepertiga apikal saluran akar yang dipreparasi denganinstrumen kontinyudanresiprokalmempunyai tingkat kehalusan yang sama

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ABSTRACTTherefore reciprocal instruments have been developed The aim was to comparethe root canal wall smoothness at the apical third between continuous rotation andreciprocal instruments Methods Thirty two mandibular first premolars weredivided into Group 1 continuous rotation instruments preparation Group 2 reciprocal instruments preparation The smoothness was measured using surfaceroughness tester The data was analyzed using independent T test Result Thedifference between groups were not statistically significant p 0 739 Conclusion Continuous rotation and reciprocal instruments have no difference inthe root canal wall smoothness at the apical third "

"Latar Belakang: Ekstrak biji anggur (GSE) mengandung 74-78% proantosianidin yang berfungsi sebagai pengikat silang kolagen. Tujuan: menganalisis kemampuan GSE sebagai larutan irigasi saluran akar dalam membersihkan smear layer pada daerah sepertiga apeks. Metode: lima puluh gigi dibagi menjadi lima kelompok. Kelompok 1 meggunakan aquades, kelompok 2 menggunakan GSE 3.25%, kelompok 3 menggunakan GSE 6.5%, kelompok 4 menggunakan GSE 13% dan kelompok 5 menggunakan EDTA 17%. Kemudian dilakukan pemeriksaan tingkat kebersihan sepertiga apeks menggunakan SEM dan diberi skor. Analisis data menggunakan uji kolmogorov-smirnov. Hasil: Skor 0 terbanyak pada GSE 13% (40%), skor 1 terbanyak pada GSE 6.5% (70%) dan skor 2 terbanyak pada aquades (80%). Kesimpulan: Larutan ekstrak biji anggur mempunyai potensi dapat membersihkan smear layer pada daerah sepertiga apeks dinding saluran akar.

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Background: Grape seed extract (GSE) consisting of 74-78% proanthocyanidin, is a cross linking agent.

Purpose: investigate the ability of GSE in removing smear layer on apical third of root canal wall.

Materials and Method: fifty five extracted incisors were divided into 5 groups. Grup 1 used aquadest, group 2 used 3.25% GSE, group 3 used 6.5% GSE, group 4 used 13% GSE and group 5 used 17% EDTA. The cleanliness of smear layer were evaluated by SEM and scored. The data were analyzed using kolmogorov-smirnov test.

Results: Score 0 maximum in group GSE 13% (40%), score 1 maximum in group GSE 6.5% (70%) and score 2 maximum in group aquadest (80%).

Conclusion: Grape seed extract solution has a potential to remove smear layer on apical third of root canal wall."

"Tesis ini membahas mengenai bagaimana tingkatan sensualitas pada suatu iklan yang dicerminkan dalam high, medium, dan low sensuality serta penggunaan model sensual tunggal (single) dan berpasangan (couple) berpengaruh pada variabel-variabel yang mengacu pada cognitive response model yang terdiri dari variabel source thought, message thought, ad execution thought, attitude toward brand (Ab), attitude toward ad (Ad), dan purchase intention. Penelitian ini merupakan penelitian eksperimen yang dilakukan dengan pemisahan kelompok melalui factorial design 3x2 (tiga tingkat sensualitas dan dua jenis penggunaan model). Dengan jumlah total responden 210 responden yang keseluruhannya adalah wanita dengan 35 reponden pada masing-masing sel, diketahui adanya perbedaan rata-rata yang signifikan terhadap Ab, Ad, dan minat beli dengan keterangan bahwa semakin rendah tingkat sensualitas maka semakin tinggi rata-rata yang diperoleh untuk ketiga variabel tersebut. Sementara itu untuk hasil perbandingan rata-rata antara model single dan couple banyak variabel yang dinyatakan tidak berbeda secara signifikan. Hal ini dikarenakan penggunaan model mengarah pada bagian dari source yang merupakan variabel kognitif. Sementara itu diketahui bahwa pengaruh variabel-variabel kognitif terhadap variabel sikap tidak mempunyai pengaruh yang cukup besar apabila dibandingkan pengaruh sikap terhadap minat beli untuk kasus iklan dengan sensual appeals menurut sudut pandang wanita.

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This research discusses how the sensuality level in an advertisement which is reflected in high, medium, and low sensuality along with the utilization of single and couple sensual model affects the variables, which refer to cognitive response model consisting of source thought, message thought, ad execution thought, attitude toward brand (Ab), attitude toward ad (Ad), and purchase intention. This is an experimental research which is applying group separation through factorial design 3x2 (three levels of sensuality and two types of model utilization). After receiving answers from 35 respondents in each cell, the research found that there is an average significant difference toward Ab, Ad, and purchase intention, with additional information that the lower the sensuality levels, the higher the average value to the three variables mentioned above. Furthermore, the comparison between the single and couple model shows that there are many variables which are not significantly different. This is caused by the model utilization which is directed to the source part which is a cognitive variable. In addition, the research also shows that there is no significant effect of the cognitive variables toward attitude variable, comparing to the effect toward purchase intention for the case of sensual appeals advertisement in woman's point of view."

"[ABSTRAKResin komposit merupakan bahan restorasi direk dengan teknik aplikasi yang sangat sensitif. Penelitian ini bertujuan untuk menganalisis efek kontaminansi saliva dan darah setelah etsa terhadap kekuatan ikat geser antara resin komposit dan email. Dua puluh lima spesimen email (n=25) secara acak dibagi dalam lima kelompok berdasarkan bahan kontaminasi dan metode pembersihan kontaminan. Hasil penelitian menunjukkan nilai rerata kekuatan ikat geser tertinggi adalah kelompok kontrol, sedangkan yang terendah adalah kelompok kontaminasi darah dengan etsa ulang. Antara kelompok kontrol dengan kelompok kontaminasi terdapat perbedaan yang signifikan. Sebagai kesimpulan, kontaminasi saliva dan darah menyebabkan penurunan kekuatan ikat geser antara resin komposit dan email;Composite resin is a direct restorative material with highly sensitive application technique.ABSTRACTThis study aimed to analyze the effect of saliva and blood contamination after etching procedure to shear bond strength between composite resin and enamel. Twenty five samples of enamel (n=25) were randomly divided into five group based on contamination agent and decontamination procedures. The result showed the mean value of shear bond strength was highest on control group and the lowest on blood contamination group with re-etching procedure. Between control group and contamination group were significantly different. In conclusion, saliva and blood contamination can decrease shear bond strength between composite resin and enamel, Composite resin is a direct restorative material with highly sensitive application technique. This study aimed to analyze the effect of saliva and blood contamination after etching procedure to shear bond strength between composite resin and enamel. Twenty five samples of enamel (n=25) were randomly divided into five group based on contamination agent and decontamination procedures. The result showed the mean value of shear bond strength was highest on control group and the lowest on blood contamination group with re-etching procedure. Between control group and contamination group were significantly different. In conclusion, saliva and blood contamination can decrease shear bond strength between composite resin and enamel]"

"Latar Belakang: Ekstrak Biji Anggur EBA mengandung proanthosianidin PA yang berperan sebagai pengikat silang kolagen yang menentukan sifat mekanis dentin. Tujuan: menganalisis pengaruh EBA dengan kadar PA 2 9 terhadap kekerasan mikro dentin saluran akar. Metode: 50 gigi dibagi menjadi 3 kelompok yang direndam larutan EBA larutan NaOCl 3 dan aquabides Dilakukan pengukuran nilai kekerasan mikro metode Vickers Analisis data menggunakan uji Kruskal Wallis. Hasil: Nilai kekerasan mikro tertinggi pada kelompok EBA dan terendah kelompok NaOCl 3 Tidak terdapat perbedaan bermakna nilai kekerasan mikro kelompok EBA 2 9 dan aquabides p 0 05. Kesimpulan: Larutan EBA dapat mempertahankan kekerasan mikro dentin saluran akar.

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Background: Grape Seed Extract GSE contains proanthosianidin PA as collagen cross linking agent that determine dentin mechanical properties.

Aim: To analyze GSE with 2 9 PA effect on root canal dentin microhardness.

Method: 50 teeth divided into 3 groups which immerse in GSE NaOCl 3 and aquabides Microhardness value measured with Vickers method Data analyze with Kruskal Wallis.

Result: The highest microhardness value on GSE group and the lowest on NaOCl group No significant difference in microhardness value of GSE group compared to aquabides group p 0 05.

Conclusion: GSE solution maintain microhardness value of root canal dentin."

"Resin komposit termasuk dalam material restoratif direk yang proses pengerasannya melalui proses polimerisasi dibantu dengan bantuan cahaya. Penelitian ini bertujuan untuk mengetahui pengaruh jarak dan durasi penyinaran terhadap kekerasan permukaan resin komposit nanofilled. 60 spesimen resin komposit yang ditumpat dalam mold dibagi ke dalam 6 kelompok berdasarkan jarak dan durasi penyinaran yang berbeda yaitu 2 mm, 5 mm, dan 8 mm; serta 20 detik dan 40 detik. Rerata nilai tertinggi dimiliki oleh kelompok dengan jarak terkecil dan durasi penyinaran terlama sedangkan nilai rerata terendah terdapat pada kelompok dengan jarak terbesar dan durasi penyinaran tersingkat. Terdapat perbedaan bermakna antar tiap kelompok perlakuan dengan jarak yang berbeda, namun pada 2 kelompok yang memiliki jarak tip LED Light Curing Unit sebesar 8 mm, tidak ada perbedaan bermakna. Dapat disimpulkan, jarak tip LED Light Curing Unit dan durasi penyinaran berpengaruh terhadap kekerasan permukaan resin komposit nanofilled. Pengaruh durasi penyinaran hanya signifikan apabila jarak tip LED Light Curing Unit terhadap permukaan resin komposit kurang atau sama dengan 5 mm.

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Composite resin is a material of choice for direct restorations. Hardening process of composite resin is triggered by light to begin the polymerization process. The aim of this research was to assess the effect of LED light curing unit's tip distance and curing time to surface hardness of nanofilled composite resin. 60 specimens were prepared into a mold and they were divided into 6 groups based on the different curing distance and time which is 2 mm, 5 mm, and 8 mm along with 20 seconds and 40 seconds. The highest surface hardness was seen in group with 2 mm tip's distance and 40 seconds curing time, while the lowest was seen in group with 8 mm tip's distance and 20 seconds curing time. Significant differences were seen between different tip's distances but in 2 groups which has 8 mm tip's distances and different curing time, there is no significant differences. In coclusion, LED light curing unit's tip distance and curing time is affecting surface hardness of nanofilled composite resin. Curing time only affect the surface hardness if the tip's distance is less than 8 mm."